Original title: Oligonucleotides modified with acyclic nucleoside phosphonate (HPEP) units
Authors: Kaiser, Martin Maxmilian ; Novák, Pavel ; Rosenbergová, Šárka ; Poštová Slavětínská, Lenka ; Rosenberg, Ivan ; Janeba, Zlatko
Document type: Papers
Conference/Event: Symposium on Chemistry of Nucleic Acid Components /16./, Český Krumlov (CZ), 2014-06-08 / 2014-06-13
Year: 2014
Language: eng
Abstract: Two acyclic nucleoside phosphonates bearing 9-[3-hydroxy-2-(phosphonoethoxy)propyl] (HPEP) moiety and either adenine and thymine as nucleobase were synthetically converted into a suitable building blocks for the subsequent automated solid phase synthesis of modified oligonucleotides. Phosphoramidite chemistry was used for the synthesis of a series of complementary nonamers where the modified acyclic monomers were incorporated using the phosphotriester method. Determination of thermal characteristics of the complexes of the modified nonamers with the complementary strands revealed a destabilizing effect of the introduced acyclic modifications.
Keywords: acyclic nucleoside phosphonate; HPEP; oligonucleotides
Project no.: TA03010598 (CEP)
Funding provider: GA TA ČR
Host item entry: Chemistry of Nucleic Acid Components. 16th Symposium, ISBN 978-80-86241-50-0

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0234287

Permalink: http://www.nusl.cz/ntk/nusl-174431


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers