Ústav živočišné fyziologie a genetiky

Nejnovější přírůstky:
2017-08-02
12:52
Role of planar cell polarity pathway in ameloblastoma with focus on frizzled 6
Putnová, Barbora ; Putnová, Iveta ; Hrubá, Eva ; Dosedělová, Hana ; Štembírek, Jan ; Daněk, Z. ; Buchtová, Marcela
Ameloblastoma is the most common epithelial odontogenic tumour in the oral cavity. Its progression is usually slow and the surface is covered by an oral mucosa with physiologic appearance. These tumours do not cause any pain to the patient and therefore it is difficult to diagnose them on time and the first signs are often missed by clinician leading to late diagnosis.

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2017-08-02
12:52
Mechanism of complex tooth shape development in reptiles
Landová, Marie ; Zahradníček, Oldřich ; Dosedělová, Hana ; Kavková, M. ; Zikmund, T. ; Buchtová, Marcela
Tooth shape formation in mammals is well known proces thanks to the broad studies on the mouse molars. The main role in regulation of this proces plays the enamel knot. Enamel knots are formed by cluster of cells, which serves as a source of numerous signalling molecules such as SHH or members of WNT, BMP and FGF families. Almost all our knowledge about teeth came from study on the mouse model. For better understanding of odontogenesis, it is however necessary to compare developmental processes to another species. One of the most heterogenic group of dentition type and shape are reptiles. How the shape of their teeth completed is still unknown. Aim of our study is therefore to uncover developmental processes involved in cusp formation in non-mammalian species with focus on reptiles. As model species for this study, we selected chameleons where multicuspis teeth develop in the caudal area of the jaw.

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2017-08-02
12:52
The effect of WNT5A and WNT7A on cartilage formation and anterior-posterior patterning of the limb
Killinger, Michael ; Buchtová, Marcela
Growth and patterning of the vertebrate limb is controlled by signals produced in the appical ectodermal ridge (AER) and by the zone of polarizing aktivity (ZPA). limb development requires tight interactions between these signalling centers to keep asymmetrical arrangement of all skeletal elements and their growth.

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2017-08-02
12:52
Role of FGF signaling in zeugopod development
Buchtová, Marcela ; Celá, Petra ; Horáková, D. ; Krejčí, P.
The fibroblast growth factor (FGF) signalling systém regulates many developmental processes including skeletogenesis, brain patterning, branching morphogenesis or limb development. as FGFs are key players in the variable processes of chondrogenesis, we experimentally manipulated this pathway to test its effect on zeugopode modeling.

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2017-03-10
16:12
Evaluation of efficacy and bio-distribution of AAV5-miHTT in HD minipig brain
Bohuslavová, Božena ; Juhás, Štefan ; Juhásová, Jana ; Ellederová, Zdeňka ; Motlík, Jan
Healthy minipigs (n=29) were pre-screened for serum AAV5 neutralizing antibodies (NABs) in serum by uniQure. Conclusions: Intrastriatal/intrathalamic bilateral application of AAV5-CAG-miHtt(3e13/1e13gctotal) or PBS/Sucrose (54μl/216μl) similarly to AA5-CAG-GFP didn´t cause any neurological deficit in transgenic as well as wild type animals. The body weight increased in all animals 84 days after AAV5-CAG-miHtt/PBS-Sucroseintrastriatal/intrathalamic delivery with two exceptions TGanimal L616 (AAV5-CAG-miHtt1e 13g ctotal) and WTanimal L485 (AAV5-CAG-miHtt3e13gc total). The TG boar N176 from group VII (PBS/Sucrose) died during narcosis 56 days after IP/IT application), shortly after CSF and blood collection.The boar breathed heavily and intermittently, then the heart stopped beat and resuscitation failed. We immediately flushed them by 20 L of PBS with heparin and collect all tissues. All animals had normal food consumption. Inflammatory proces in AAV5-CAG-miHtt (1e13 gc total) seems to be similar to PBS/Sucrose injected animals accompanied with no detectable histological pathologies.

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2017-03-10
16:12
Phenotypic Analyses of the HD transgenic Minipig Model
Motlík, Jan ; Ellederová, Zdeňka
The transgenic model of Huntington’s disease in minipig (TgHD) was created in 2009 and information coding the sequence of N-terminal part of human mutated huntingtin was transferred to subsequent generations from both female and male sides. In each litter, transgenic (TgHD) and wild-type (WT) piglets were born in approximately equal ratio. At present, the Laboratory of Cell Regeneration and Plasticity keeps sets of animals in F2 and F3 generations with identical genetic background and bred in identical conditions of feeding and housing. The present research project was focused on a complex of non-invasive and invasive approaches to WT and TgHD minipigs to achieve the entire phenotypic analysis of HD progression in this large animal model.

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2015-01-26
15:35

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2015-01-19
14:10
E-INFRASTRUCTURES FOR RESEARCH AND INNOVATION: LINKING INFORMATION SYSTEMS TO IMPROVE SCIENTIFIC KNOWLEDGE PRODUCTION
Ráb, Petr
The workshop gives an overview about CRIS in the Czech Republic and Slovakia. It consists of a number of short presentations that mention important aspects and current question about CRIS in the two neighbouring countries. Research evaluation is among the important topics as well.

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2015-01-19
14:10

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2015-01-05
14:24
Metodika vypracování akutního a transgenního modelu Huntingtonovy choroby u miniaturních prasat a jeho aplikace pro testování nových léčebných postupů a léčiv v oblasti neurodegenerativních chorob
Motlík, Jan ; Juhás, Štefan ; Juhásová, Jana ; Vodička, Petr ; Baxa, Monika
Cílem metodiky je vypracování biomedicínského modelu Huntingtonovy choroby u miniaturních prasat a jeho využití v preklinické praxi pro testování nových léčebných postupů jak farmakologických, tak pomocí nových molekulárně biologických metod.

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