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The function and role of profit in the company
VRZÁKOVÁ, Tereza
To evaluate the role and importance of its profits in the corporate practice of measuring, mapping the factors affecting its size. On the basis of the financial statements of the chosen company to analyze the time evolution of the profit resp. development of it designed ratios for 3-5 years.
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Aspects of the Pastoral Care in the Pauline Congregations
Ravasz, Hajnalka ; Pokorný, Petr (advisor) ; Theißen, Gerd (referee) ; Luz, Ulrich (referee)
The thesis "Aspects of the Pastoral Care in the Pauline Congregations" presents some aspects of the praxis of pastoral care in the graeco-roman Antiquity and in the New Testament how it is principally revealed in the 1st Thessalonians. The main chapters of the thesis expose the chosen aspects of pastoral care like the function of imitation and examples, the role of the pastor in the pastoral act, the process of comforting or the caring congregations. But the research does not end with the comparison of the antic psychologic tradition with the Pauline practise; at least one modern social-psychological method is introduced in every chapter and they are used as a help for a different and sometimes maybe astonishing re-reading of the well-known Bible texts. The social connections analyzing social psychology helps the New Testament finding new methods and language to express the caring interactions between Paul and his congregations. This interdisciplinary thesis aims after binding the biblical texts with their contemporary context and the modern social psychology to reach a helping hand for the practical theology, mainly for pastoral care drawing the conclusion from the exegesis for our eneryday practise. Powered by TCPDF (www.tcpdf.org)
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Internal Migration in PRC: function, role and impact on Chinese economy
Čermáková, Zuzana ; Stuchlíková, Zuzana (advisor) ; Procházková Ilinitchi, Cristina (referee)
The purpose of this thesis is to analyze the process of internal migration in Peoples' Republic of China. It focuses on low-skilled rural migrant workers, their overall characteristics, role in the economy and their status within Chinese society. This analysis aims to prove that despite the fact that rural migrants represent an essential element of Chinese economy, and have contributed by a great deal to China's economic take-off, they are still a very discriminated group of Chinese society and are constantly facing violations of their rights.
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Post-penitentiary treatment and prevention of recidivism
Hrnčíř, Luděk ; Vokoun, Rudolf (advisor) ; Vanduchová, Marie (referee)
RÉSUMÉ The work presented is a Master thesis completed at the Criminal law department under theLaw faculty of the Charles' University. It deals with recidivism, its causes and prerequisites of its occurrence. Preventive activities in the Czech republic are also a part of this project. Emphasis is put especially ontertiary preventive activities, namely working with people who have commited crime before. Postpenitential care plays an important role here, especially the problems of returning the convicted person into normal life. For synoptic reasons, the text also contains the overview of the Czech legal regulations, especially the standards of the criminal law. The standards of the social security law, which determine the system made by the state for people exposed to social exclusion, are also dealt with here. Apart from the national legislative, the international regulations are also mentioned here, although they are rather informal recommendations of the EU. A special chapter is devoted to Czech prison system. Conditions in Czech jails are described as well as programme set up for dealing with prisoners and also the difficulties of so called exit departments. The most important part of the thesis contains information about the state and opportunities of the postpenitential care. These problems occur in...
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The role of ATM in breast cancer
Soukupová, Jana ; Pohlreich, Petr (advisor) ; Souček, Pavel (referee) ; Foretová, Lenka (referee)
Incidence of breast cancer is continuously increasing in the Czech Republic. Tumor development is a result of gene alterations'accumulation, particulary associated with genes involved in regulation of cell growth and division. Hereditary carcinomas account for approximately 5-10% of all breast tumors and in 60-80% cases are caused by a germline mutation in the major predisposition genes BRCA1 and BRCA2. Nevertheless, other genes, mostly of lower penetrance, may play a role in breast pathogenesis such as the ATM tumor-suppressor gene. ATM is the apex of the repair pathway of DSB. This protein kinase activates through phosphorylation of its substrates cell cycle checkpoints, which leads either to the delay of the cell cycle progression until DSB are repaired or to the promotion of apoptosis. To sum up, the ATM gene seems to have a role in breast cancer development in a minority of the high-risk families in our population which is significantly lower compared to BRCA1/2 and it also seems to be involved in pathogenesis of sporadic breast cancer. Despite the ATM gene's length, we do not perform the preventive screening of this gene in breast cancer high-risk families. Nevertheless, we offer the molecular diagnostics of ATM to ataxia telangiectasia patients.
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Importance of Transport Proteins and Biotransformation Enzymes for Defending Role of the Placenta
Vacková, Zuzana ; Štaud, František (advisor) ; Trejtnar, František (referee) ; Mičuda, Stanislav (referee)
Placenta is a unique organ which ensures a number of vital functions necessary for normal course of pregnancy and development of a new individual. In addition to its main function of oxygen supply and nutrient and waste product exchange, placenta also serves as an endocrine, metabolic and protective organ. Placenta is considered to be one of the physiological barriers of the organism which regulates transport of both endogenous and exogenous compounds between two compartments - maternal and fetal blood circulations. Up to recently, the placental barrier was supposed to be formed only by cellular layers which separate maternal and fetal blood - syncytiotrophoblast and fetal capillary endothelium. However, it has been demonstrated that the activity of placental efflux transport proteins and metabolic enzymes contributes considerably to the protective function of placental barrier. Efflux transporters are membrane proteins which actively (along with consumption of ATP) "pump" a diversity of substrates out of the cell. It has been shown that the kinetics of transport of various substances across the placenta is affected predominantly by two transporters: P-glycoprotein (P- gp) and breast cancer resistance protein (BCRP). Compared to these transporters, placental biotransformation enzymes are considered...
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Antibodies against sand flies saliva in domestic animals from endemic areas of visceral leishmaniasis
Košťálová, Tatiana ; Volf, Petr (advisor) ; Kopecký, Jan (referee)
The main aim of this thesis was to test sera of domestic animals from endemic areas of visceral leishmaniasis in north and northwest Ethiopia for antibodies against presumed vector P. orientalis salivary glands and to clarify behavior of the sand fly and the role of domestic animals in transmission of visceral leishmaniasis. Specific IgG antibodies against P. orientalis saliva were tested in dogs, cattle, goats, sheep and donkeys. Above the cut-off value there was 76 % analyzed dogs, 15 % cattle, 26 % goats, 60 % sheep and 45 % donkeys. Dogs, goats and donkeys revealed significantly higher anti-P. orientalis antibodies compared to control animals. In the case of cattle, goats and donkeys cut-off levels were very high and is questionable if observed results are relevant. Canine sera were screened also for anti-P. orientalis IgG2 and 88% of Ethiopean sera revealed significantly higher IgG2 than control sera. The next aim was to detect if canine sera from Ethiopia with anti-P. orientalis antibodies react with other bloodsucking insect saliva by using immunoblotting method. In saliva of sand fly Sergentomyia schwetzi, which is abundant in Ethiopia, canine sera reacted mostly with 36, 37 and 51 kDa proteins. Mosquito Culex quinquefasciatus is cosmopolitan species and canine sera most intensely...
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The Role of Gut Microbiota and Lipopolysachaide Content of the Diet in the Development, Maturation and Function of the Immune System
Hrnčíř, Tomáš ; Tlaskalová - Hogenová, Helena (advisor) ; Prokešová, Ludmila (referee) ; Macela, Aleš (referee)
Mammals are essentially born germ-free but the epithelial surfaces are promptly colonized by astounding numbers of bacteria soon after birth. The most extensive microbial community is harboured by the distal intestine. The gut microbiota outnumbers ~10 times the total number of our somatic and germ cells. The hostmicrobiota relationship has evolved to become mutually beneficial. Studies in germfree mice have shown that gut microbiota is essential for the proper development of the immune system. The pivotal role of the innate immune system in the complex and dynamic host-microbiota interactions has become increasingly evident. The principal aims of the present study were: firstly, to determine whether LPS-rich sterile diet can promote maturation of the immune system in germ-free mice, secondly, to elucidate whether gut microbiota and LPS-rich sterile diet influence the LPS susceptibility, and finally, to investigate a role of the adaptive immunity in endotoxin shock. Our data clearly show that both live gut microbiota and LPS-rich sterile diet increase susceptibility to endotoxin shock. Further, we demonstrate that immunodeficient SCID mice, which lack mature B and T cells, are more sensitive to endotoxin shock than immunocompetent Balb/c mice. In addition, we show that not only live gut microbiota but also...
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Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Ledvina, Vojtěch ; Klepárník, Karel
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.
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Study of the composition and organization of cytochrome P450 system by covalent crosslinking
Koberová, Monika ; Hodek, Petr (advisor) ; Hansíková, Hana (referee)
The system of mixed function oxygenase (MFO system) participates in significant roles in the metabolism of endogenous compounds and xenobiotics. This system contains cytochrome P450, NADPH:cytochrome P450 reductase, and also there are assigned NADH: cytochrome b5 reductase and cytochrome b5. It was proved that cytochrome b5 can stimulate or inhibit cytochrome P450 (CYP)-dependent reactions and even change the ratio of resulting metabolites. The mechanism of cytochrome b5 action has not been fully elucidated yet. Elucidation of protein-protein interactions in MFO system and determination of topology of this system could explain the mechanism of cyt b5 action. The covalent cross- linking technique is suitable method for identifying protein-protein interactions within the membrane. Cytochrome b5 contains 3 methionines and in 2 cases the methionines are localized in a short hydrophobic C-terminal membrane anchor. Interactions with cytochrome P450 in the membrane environment can be identified by substitution of two methionine for photoactivatable analogue of methionine (photo-methionine) and subsequent photoactivation. This work is focused on expression and isolation of photo-cytochrome b5 (photo- cyt b5), cytochrome b5 analogue with incorporated photo-methionine. Conditions for photo-methionine...
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