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Multiphoton and non-linear Raman microscopy through a multimode fibre
Pikálek, Tomáš ; Marte, Monika Ritsch- (referee) ; Zemánek, Pavel (referee) ; Čižmár, Tomáš (advisor)
Multimodová vlákna je možné využít jako miniaturní endoskopy pro zobrazování hluboko uvnitř mozku myších zvířecích modelů s minimálním poškozením okolní tkáně. S využitím metod tvarování vlnoplochy je možné svazek vystupující z vlákna fokusovat do jediného bodu, a vytvořit tak miniaturní skenovací mikroskop. Různé mikroskopické zobrazovací metody již byly tímto způsobem implementovány, a to včetně několika in-vivo demonstrací. Tato práce se zabývá implementací zobrazování pomocí koherentního anti-Stokesova Ramanova rozptylu (CARS), nelineární metody umožňující zobrazování s chemickým kontrastem bez značení vzorku. Tato metoda má potenciální využití pro klinické aplikace například při diagnóze nádorů. Práce je zaměřena zejména na optimalizaci endoskopu a vláknových sond pro fokusaci světla z pulzních laserů včetně kontroly disperze s cílem umožnit nelineární zobrazování. Takto optimalizovaný systém je následně využit pro demonstraci zobrazování metodou CARS uvnitř tkáně (například lipidové dvojvrstvy buněčných membrán kolem axonů) a chemického kontrastu na polymerových kuličkách. Systém je též použit k demonstraci multimodálního zobrazování fixované myší tkáně kombinací CARS a dvoufotonové mikroskopie.
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Strategies for providing care within a selected endoscopic procedure
ŠAVRDOVÁ, Soňa
The diploma thesis deals with a strategy of providing care within the framework of a colonoscopy and gastroscopy procedure. The aim of this thesis is to find out what information is provided by the nurses to the patients during a colonoscopy and gastroscopy procedure, what communication techniques are used by the nurses within the framework of reducing the fear at the patients and what expectations the patients have from the nurses before the colonoscopy and gastroscopy procedure. Six research questions were established, which surveyed what information is provided by the nurses to the patients during the procedure, what communication techniques are used by the nurses within the framework of reducing the fear at the patients and what expectation the patients have from the nurses. Furthermore, they surveyed what kinds of problem have both the nurses and patients to solve and how the nurses secure the patients´ intimacy within the framework of the colonoscopy and gastroscopy procedure. In the theoretical part, the thesis is focused on the anatomy and physiology of the digestive system, progress of endoscopic devices, on endoscopy procedures and nursing process within the framework of the colonoscopy and gastroscopy procedure. And at last but not least, the security of the patient´s intimacy and a role of a nurse - communicator within the framework of the colonoscopy and gastroscopy procedure. To achieve the aim, the qualitative method of research construction was used with a method of an interview, face-to-face technique at the patients undergoing the colonoscopy and gastroscopy procedure and at the nurses providing these patients with nursing care. The research construction was supplemented with the nurses´ observation. The results from the obtained data led to the conclusion that the nurses ensure the patients´ intimacy properly, use the communication techniques for reducing the fear efficiently, provide the patients with information concerning the endoscopy procedure and that they rarely experienced any problems. Furthermore, the research construction revealed that a varied part of the patients never experienced any problems and their positive expectation from the nurses was fulfilled. The results of the research construction will be offered to the employees of the endoscopy department where the research was performed as a feedback for the approach to the patients within the framework of the colonoscopy and gastroscopy procedure.
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Nursing care of the patient with pancreatic carcinoma
BELLA, Inna
This bachelor thesis deals with the topic 'Nursing Care for Patients with an Oncological Disease of the Pancreas'. It maps the work of a nurse taking care of a patient, with regard to the investigative methods in proving the disease of carcinoma of the pancreas and with regard to an inpatient part of the gastroenterological department. Goals of this thesis are: 1. To find out specifics about the nursing care with respect to patients with an oncological disease of the pancreas, 2. To find out the role of a general nurse during the investigative methods regarding the oncological disease of the pancreas, 3. To find out possibilities of further education of nurses who provide nursing care for patients with an oncological disease of the pancreas. Three research questions were set beside these goals: 1. What are the specifics of a nurse caring for patients with an oncological disease of the pancreas? 2. What is the role of a general nurse during the investigative methods of an oncological disease of the pancreas?, 3. What are the possibilities of further education of nurses who provide nursing care for patients with an oncological disease of the pancreas? Part of this bachelor thesis research consists of the qualitative research investigation with the usage of a semi-structured interview. Research investigation took part in the gastroenterological department where there were situated both inpatient and endoscopic parts of the department. Endoscopic nurses were asked questions that focused on the care of a patient before, during, and after the examination. Nurses who work in the inpatient part of the gastroenterology spoke about the specifics of the nursing care of a patient with carcinoma of the pancreas. Endoscopic nurses agreed that the most important part in their work is the usage of endoscopic devices and instrumentarium, their disinfection and sterilization. Also, they agreed that equally important are preparation and care of a patient during and after an examination and an assistance to a doctor during the examination. Nurses in the inpatient department who take care of a patient with carcinoma of the pancreas state that their nursing care focuses on the preparation of a patient for examination, on dealing with pain and nutrition with respect to the patient, and also on a quality of nursing care in a terminal stage. Results of this bachelor thesis could be used as a study material for students who deal with this topic and especially for new nurses in the gastroenterological department.
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Nurse's work at gastroenterological out-patient's ward
KOTKOVÁ, Stanislava
This bachelor work "Nurse's work at gastroenterological out-patien's ward" maps the work of nurse at gastroenterological out-patient's ward, the nurse needs to have a technical qualification, needs to know how to organize her work and how to take care of a patient and how to communicate. The work of gastroenterological nurse is really challenging according to constantly developing technologies. The theoretical part describes nursing methods and specifics of nurse's work. The research part was realized through qualitative survey via semi-structured discussion. The aims of the work were: 1. To find specialties of the nurse's work at gastroenterological out-patient's ward and 2. Figure out choices in further education of nurses working at gastroenterological out-patient's ward. There were stated three research questions: 1) What is the content of the nurse's work at gastroenterological out-patient's ward? 2) What is specific about the communication between the nurse and the patient? 3. What possibilities do nurse working at the gastroenterological out-patient's ward have in further education? Although the research was made in two different hospitals, nurses almost agreed on the content of their work. The survey showed that nurses do a lot of work: they guarantee the functioning of out-patient's ward, they communicate with the doctor and the patient, they watch out for endoscopes, they take biological material etc. We found out that nurses have a lot of opportunities for further education which hospital offers such as courses, seminars and workshops. The results of this work could be used as a source of information for nurses working at gastroenterological out-patient's ward or as a studying material for new coming nurses. These results could also be presented in professional magazines and in professional conferences.
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