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A foundation plan of karate sport club
Trnková, Lucie ; Pavelka, Radim (advisor) ; Stich, Jaroslav (referee)
Title: A FOUNDATION PLAN OF KARATE SPORT CLUB Aims: The aim is to explain how to establish a karate sport club in new terms of foundation of associations. Another goal is the application of the theoretical knowledges to a chosen karate club Red Dragon Team (RDT) and to design options how to improve its functioning. Methods: Based on expert literature and texts research, a study has been produced which describes basic terms and ways of analysis execution. The SWOT analysis of the current state of the inner and outer environment of the RDT sports clubwas used in the second section of this document. Results: The work provides comprehensive guide describing the particular steps for founding of a new association in practice. The work is proposing a solution based on the SWOT analysis to eliminate possible deficiencies and how to better use the potential of the club RDT. Keywords: association, karate, sport club, management, SWOT analysis
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Modification of glutathione S-transferase by selected glycating agents
Průchová, Zuzana ; Dršata, Jaroslav (advisor) ; Trnková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Zuzana Průchová Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Title of diploma thesis: Modification of Glutathione S-transferase by selected glycating agents Glycation is a non-enzymatic reaction, which leads to the binding of carbonyl compounds to free amino groups of amino acids, peptides, proteins or other biomolecules. This process is closely related to impaired protein function in diabetes mellitus, during aging, and in neurodegenerative diseases. The main purpose of this thesis was to monitor the impact of several glycating agents on the properties of the enzyme glutathione S-transeferase (GST, EC 2.5.1.18) which plays a key role in the detoxification of many exogenous as well as endogenous compounds. Methylglyoxal (0.5-2 mM), glyceraldehyde (0.5-10 mM), glucose (50 mM), and fructose (50 mM) were selected as glycating agents. The course of protein glycation was evaluated by following methods: GST catalytic activity assessment, determination of primary amino groups, assessment of AGEs formation using fluorescence and Western blotting, generation of high molecular cross-links and aggregates by denaturationg polyacrylamide gel electrophoresis (SDS-PAGE), changes in GST molecular...
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MacPherson Suspension Kinematics Analysis
Trnková, Lucie ; Fojtášek, Jan (referee) ; Hejtmánek, Petr (advisor)
This bachelor thesis deals with the influence of the position of significant points of MacPherson strut on kinematic characteristics. The first part contains a description of selected suspension parameters, their impact on the driving properties of the car, and a description of MacPherson strut. Furthermore, based on provided data, is prepared a detailed analysis of the influence of kinematics points to individual characteristics. In conclusion, all findings are synoptically summarized so that they can serve as a manual for change of the kinematic characteristics.
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Substitute family care in the Czech Republic
Trnková, Lucie ; Salač, Josef (advisor) ; Hendrychová, Michaela (referee) ; Radvanová, Senta (referee)
This dissertation is concerned with the issues surrounding substitute family care in the Czech Republic and presents an analysis of its legal regulation as set out in both private law and public law regulations. The term substitute family care encompasses several independent legal institutions, which together form a system serving to protect children at risk who cannot grow up in their own family. The system of substitute care is divided into family-type individual care on the one hand and collective care provided in institutional facilities on the other. This dissertation examines the historical development of forms of substitute care, analyses their current legal regulation, including procedural regulation, and evaluates the interconnectedness and mutual harmoniousness of individual legal regulations. It analyses the inner workings of the substitute care system and delineates the rights and obligations of entities which enter into it in various capacities, with an emphasis on how the fulfilment of the rights of a minor is enforced and how their best interest is considered. Given the subject matter, the topic of the dissertation is approached in an interdisciplinary fashion, for the interdisciplinary cooperation of individual persons acting on behalf of the state in the legal relations of...
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A foundation plan of karate sport club
Trnková, Lucie ; Pavelka, Radim (advisor) ; Stich, Jaroslav (referee)
Title: A FOUNDATION PLAN OF KARATE SPORT CLUB Aims: The aim is to explain how to establish a karate sport club in new terms of foundation of associations. Another goal is the application of the theoretical knowledges to a chosen karate club Red Dragon Team (RDT) and to design options how to improve its functioning. Methods: Based on expert literature and texts research, a study has been produced which describes basic terms and ways of analysis execution. The SWOT analysis of the current state of the inner and outer environment of the RDT sports clubwas used in the second section of this document. Results: The work provides comprehensive guide describing the particular steps for founding of a new association in practice. The work is proposing a solution based on the SWOT analysis to eliminate possible deficiencies and how to better use the potential of the club RDT. Keywords: association, karate, sport club, management, SWOT analysis
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The popularity of the sport among the general public in relations to its presentatiton on TV
Trnková, Lucie ; Čáslavová, Eva (advisor) ; Štědroň, Bohumír (referee)
Title: The popularity of the sport among the general public in relations to its presentation on TV Objectives: The aim of this thesis is to determine the popularity of the sport among the general public and to compare it with its presentation on TV. The popularity and viewership of the sport is determined by questioning the public. To conduct interviews with experts on sports broadcasters from television, explaining views of sport news provided on public and commercial televisions. Final results were compared with the results of observation presentation of the sport in sport news in the thesis of Trnková (2014). The results allowed to propose recommendations which will be for use in marketing, sports and media organisations. The results will only imply a certain degree of scientific contribution. Methods: The market research of this thesis has consisted of quantitative and qualitative methods. An electronic questionnaire published on the server Vyplňto.cz, helped gather results from the public. Interviews were carried out in-depth including electronic questioning via e-mail correspondence. The theoretical part is based on the analysis of documents. Results: Sport is very popular within the Czech population in its active and passive form. Based on research carried out, men are more interested in...
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Presentation of sports in television broadcasting in the Czech Republic
Trnková, Lucie ; Čáslavová, Eva (advisor) ; Šíma, Jan (referee)
Title: Presentation of sports in television broadcasting in the Czech Republic Objectives: The aim of this thesis is to measure and compare the airtime devoted to individual sports and sports representations on public and commercial television in the regular, prime-time sports news. The recommendations will be proposed to be used in sports-club marketing, based on the results of observations. Methods: This thesis uses, as a method of observation, the standard sport news shown at prime-time on the TV stations ČT1 and TV Nova. This is the type of indirect observation with the technical devices - USB TV tuner and PC. In terms of content, Berelson's quantitative content analysis will be used, which examines the content of media messages. The theoretical part of the thesis is based on an analysis of documents. Results: The results of observations illustratively show the different approaches between public and commercial television for the inclusion of sport in the broadcasting of regular sports news in prime-time. For both channels, the most popular sports in the sports news are football and ice hockey. In addition, there is a trend of popularity of summer sports, even in the winter time. Sports broadcasting has high frequency of airtime on public Czech television broadcasting. TV Nova focuses on the...
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Modification of glutathione S-transferase by selected glycating agents
Průchová, Zuzana ; Dršata, Jaroslav (advisor) ; Trnková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Zuzana Průchová Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Title of diploma thesis: Modification of Glutathione S-transferase by selected glycating agents Glycation is a non-enzymatic reaction, which leads to the binding of carbonyl compounds to free amino groups of amino acids, peptides, proteins or other biomolecules. This process is closely related to impaired protein function in diabetes mellitus, during aging, and in neurodegenerative diseases. The main purpose of this thesis was to monitor the impact of several glycating agents on the properties of the enzyme glutathione S-transeferase (GST, EC 2.5.1.18) which plays a key role in the detoxification of many exogenous as well as endogenous compounds. Methylglyoxal (0.5-2 mM), glyceraldehyde (0.5-10 mM), glucose (50 mM), and fructose (50 mM) were selected as glycating agents. The course of protein glycation was evaluated by following methods: GST catalytic activity assessment, determination of primary amino groups, assessment of AGEs formation using fluorescence and Western blotting, generation of high molecular cross-links and aggregates by denaturationg polyacrylamide gel electrophoresis (SDS-PAGE), changes in GST molecular...
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Interaction of Proteins with Low-molecular Substances in vitro. Effect of Glycation, Substances of Plant Origin, and Combination of these Factors on Function and Spectral Properties of Selected Proteins.
Trnková, Lucie ; Dršata, Jaroslav (advisor) ; Patočka, Jiří (referee) ; Mikšík, Ivan (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate Ing. Lucie Trnková Supervisor Prof. MUDr. Jaroslav Dršata, CSc. Title of Doctoral Thesis Interaction of proteins with low-molecular substances in vitro. Effect of glycation, substances of plant origin, and combination of these factors on function and spectral properties of selected proteins. Plant polyphenolic compounds naturally occurring in human diet possess a wide range of biological and pharmacological properties. They can interact with various proteins including enzymes. Their interaction with serum albumin has a great significance because of its ability to bind, transport and store many endogenous and exogenous low-molecular compounds present in blood circulation. One of the objectives of this thesis was to study chemical structure-binding affinity relationships of two important groups of polyphenolic substances, namely hydroxycinnamic acids and catechins (flavanols), with the molecule of serum albumin in the in vitro models using spectroscopic (UV-Vis absorption spectroscopy, fluorescence quenching method) and electrophoretic (native and SDS PAGE) methods. Some polyphenols caused the changes in protein conformation and the relationships between their structures and obtained...
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Modification of glutathione S-transferase by selected glycating agents
Průchová, Zuzana ; Dršata, Jaroslav (advisor) ; Trnková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Zuzana Průchová Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Title of diploma thesis: Modification of Glutathione S-transferase by selected glycating agents Glycation is a non-enzymatic reaction, which leads to the binding of carbonyl compounds to free amino groups of amino acids, peptides, proteins or other biomolecules. This process is closely related to impaired protein function in diabetes mellitus, during aging, and in neurodegenerative diseases. The main purpose of this thesis was to monitor the impact of several glycating agents on the properties of the enzyme glutathione S-transeferase (GST, EC 2.5.1.18) which plays a key role in the detoxification of many exogenous as well as endogenous compounds. Methylglyoxal (0.5-2 mM), glyceraldehyde (0.5-10 mM), glucose (50 mM), and fructose (50 mM) were selected as glycating agents. The course of protein glycation was evaluated by following methods: GST catalytic activity assessment, determination of primary amino groups, assessment of AGEs formation using fluorescence and Western blotting, generation of high molecular cross-links and aggregates by denaturationg polyacrylamide gel electrophoresis (SDS-PAGE), changes in GST molecular...
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