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The effect of alpha-humulene and carophyllene oxide in ovarian cancer cells
Žáková, Kateřina ; Matoušková, Petra (advisor) ; Svobodová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Kateřina Žáková Supervisor: Ing. Petra Matoušková, Ph.D. Title of diploma thesis: The effect of alpha-humulene and caryophyllene oxide in ovarian cancer cells. Sesquiterpenes α-humulene and β-caryophyllene oxide are substances with antiproliferative activity against number of tumor cell lines. In this study, the antiproliferative efect of α-humulene and β-caryophyllene oxide was tested alone and in combination with doxorubicin, using neutral red uptake assay. Doxorubicin- sensitive cell line A2780 was more sensitive to the action of doxorubicin and sesquiterpenes than SKOV3 cell line, which is naturally doxorubicin-resistant. To determine the interactions between sesquiterpenes and doxorubicin, software program CalcuSyn which is based on Chou-Talay method was used. The combination indexes ofsesquiterpenes with doxorubicin were determined. Both sesquiterpenes had antagonistic effect on doxorubicin in A2780 cell line and synergistic effect on SKOV3 cell line. The mechanism of the antiproliferative effect of all three tested substances is explained by the production of ROS. For the determination of pro-oxidant effects of the tested substances the DCF assay was used. Our results showed good...
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Impact of selected sesquiterpenes on biotransformation and antioxidant enzymes in hepatocytes and cancer cell line MCF-7
Auterská, Lenka ; Svobodová, Hana (advisor) ; Štambergová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Lenka Auterská Supervisor: PharmDr. Hana Bártíková, Ph.D Title of diploma thesis: Impact of selected sesquiterpenes on biotransformation and antioxidant enzymes in hepatocytes and cancer cell line MCF-7 A breast cancer belongs among the most common cancer diseases of women and its incidence keeps increasing. The highest percentage is represented by a ductal carcinoma, which originates in the cells of the milk ducts and from there it can spread to other tissues. Nowadays, there are several cell lines, which serve for research purposes in the field of cancer therapy as a model for in vivo and in vitro studies. Particularly the MCF-7 cell line has been proved as a significant source of information about the response of cancer tissues to drug administration. It has been proved that content components of essential oils are able to inhibit cancer cell proliferation, induce apoptosis, suppress abiogenesis and metastases, and enhance the effect of chemotherapy. Besides other things, numerous studies have confirmed their effect on the activity of enzymes involved in a body protection against free radicals and oxidative stress. The aim of this thesis was to determine the cytotoxic effect of...
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Influence of TNF-α on hENaC subunits expression
Martan, David ; Svobodová, Hana (advisor) ; Matoušková, Petra (referee)
University of Vienna Faculty of Life Sciences Department of Pharmacology and Toxicology Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: David Martan Supervisors: Prof. Dr. Rosa Lemmens-Gruber, PharmDr. Hana Bártíková, Ph.D. Title of the diploma thesis: Influence of TNF-α on hENaC subunits expression The human amiloride-sensitive epithelial sodium channel (hENaC) is a type of ion channel which has the ability to control salt and water homeostasis. Therefore it is one of the main driving forces for the reabsorption of water through the alveolar epithelium. A dysfunction of this channel, respectively of this control mechanism, leads to a very severe disease - pulmonary edema and several other pathological conditions. Previous studies tested a drug called AP301, recently named solnatide. AP301 is a cyclic protein comprising the human tumour necrosis factor lectin-like domain sequence. This drug was recently developed as a potential treatment of pulmonary edema. The principle is that it activates hENaC by increasing the open probability. It was also shown that AP301 transiently increases the expression of hENaC subunits in mammalian cells. In this study we used the Western blot method to test the influence of tumour necrosis factor α...
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The impact of selected sesquiterpenes on the effectiveness of anticancer therapy in MDA-MB-231 cell line
Tůmová, Veronika ; Svobodová, Hana (advisor) ; Malátková, Petra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Title, Name, Surname of candidate: Veronika Tůmová Title, Name, Surname of tutor: PharmDr. Hana Bártíková, Ph.D Title of a diploma work: The influence of selected sesquiterpenes on the effect of cytostatics for the cell line MDA-MB-231 Breast cancer is one of the most common forms of cancer. It makes up approximately one quarter of cancer cases amongst women. The rise in the incidence of this disease in western countries has, over recent years, been linked to a lifestyle that characteristically involves a sedentary way of life and a diet rich in animal fats. There are currently many therapeutic regimes available for the treatment of breast cancer which are increasingly adapted to the individual needs of the patient and their specific tumor subtype. In spite of the wide range of treatments with a variety of mechanisms of effects on cancer tissues, current treatments are limited by inadequate effectiveness, serious undesirable side effects or the occurrence of resistance. Scientists have therefore been attempting to uncover ways to prevent these undesired effects. Sesquiterpenes α humulene and trans-nerolidol are naturally-occuring substances that, through their biochemical effects, act cytotoxically...
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Changes of gene and protein expression of selected enzymes in mice due to cranberry antioxidants and obesity
Vášková, Lucie ; Svobodová, Hana (advisor) ; Skálová, Lenka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Lucie Vášková Supervisor: PharmDr. Hana Bártíková, PhD. Title of thesis: Changes of gene and protein expression of selected enzymes in mice due to cranberry antioxidants and obesity Obesity is a chronic disease coming up to endemic size in contemporary population. It is often associated with an adverse pathophysiological effects in the organism. This condition affects many processes occurring in the body and probably also activity and expression of antioxidant and biotransformation enzymes. Expression of antioxidant enzymes is influenced by dietary intake of antioxidants, which can be definitely beneficial to health. This thesis concerns about changes in protein and gene expressions of biotransformation and antioxidant enzymes by testing the four groups of experimental mice. This determination was carried out in normal and obese mice in which obesity was induced artificially by administration of monosodium glutamate. These individuals served as control group to the other two groups, which were used for testing the effect of cranberry extract on enzyme expression. The expressions were determined in subcellular fractions of livers and small intestine. The methods to determination of...
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The impact of selected sesquiterpenes on the effectiveness of anticancer therapy in MCF-7 cell line
Vogričová, Lenka ; Svobodová, Hana (advisor) ; Hanušová, Veronika (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Title, Name, Surname of candidate: Lenka Vogričová Title, Name, Surname of tutor: PharmDr. Hana Bártíková, Ph.D. Title of a diploma work: The influence of selected sesquiterpenes on the effect of cytostatics in the MCF-7 cell line Sesquiterpenes are natural substances with numerous biological effects. Anti-tumour effect of certain sesquiterpenes has been determined. Doxorubicin is an antracycline- based medicine used for cancer chemotherapy, among other things, for the treatment of breast cancer. The doxorubicin therapy is limited by a number of adverse effects, particularly its cardiotoxicity. A combination of doxorubicin with suitable substances might result in lower therapeutic dosages of doxorubicin. One of the alternatives is the utilization of sesquiterpenes combined with doxorubicin. The purpose of this thesis is the determination of the effect of two sesquiterpenes, α-humulene and trans-nerolidol on the MCF-7 breast cancer cell line and the determination of the effect of α-humulene and trans-nerolidol on the effect of the doxorubicin cytostatic. The effect of α-humulene, trans-nerolidol, doxorubicin and a combination thereof on cell proliferation has been evaluated by the neutral red uptake...
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Differences between susceptible and resistant strains of Barber's pole worm (Haemonchus contortus)
Bartásková, Lenka ; Svobodová, Hana (advisor) ; Lecová, Lenka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Departement of Biochemical Sciences Candidate: Lenka Bartásková Supervisor: Mgr. Hana Bártíková, Ph.D. Title of the thesis: Differences between susceptible and resistant strains of Barber's pole worm (Haemonchus contortus) Haemonchus contortus is very common parasite. It can infect a large number of ruminant species and is a very dangerous parasite of sheep and goats worldwide. The infection caused by these worms is called haemonchosis. The symptoms are anaemia, loss of weight and death of the infected sheep and goats. Nowadays we can prevent this disease. The prevention of haemonchosis is available in the form of anthelmintic drugs. The most commonly administered drugs from this category are represented by benzimidazoles. However, the frequent use of anthelmintic leads to development of drug resistance in parasites. The resistance to anthelminthic drugs is caused by mutation at codon 200 of β-tubulin. The worms Haemonchus contortus can be divided into sub-groups according to their anthelmintic resistance. This thesis compares the number of alleles responsible for resistance in sensitive and resistant strains of Haemonchus contortus. A low percentage of the resistant allele is found in the sensitive strain ISE. In the resistant...
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Art spaces in geographical contexts: the case of Karlovy Vary
Adamec, Vítězslav ; Matoušek, Roman (advisor) ; Svobodová, Hana (referee)
This bachelor thesis is devoted to art spaces in Karlovy Vary and geographical contexts related to them. By using interviews with representatives of these spaces is obtained the image of culture of Karlovy Vary from a spaces players' perspective. Geographical context was understood by various levels of the scale, from the close environs to the influence of international events. Next research question was devoted to political and economic influences on the cultural life of the city and eventually it was turned attention to the net of mutual relations between culture players in Karlovy Vary. The thesis pointed to the contradictoriness of contemporary cultural situation of the city, which is the expression of the imbalance between the free activity of art spaces and the pressure to the economic profit. Keywords: cultural geography, urban geography, art spaces, art, cultural policy, geographical context
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