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The Proposal for Development of Family Wine Shop in Prostějov
Obořilová, Tereza ; Procházková, Lenka (referee) ; Bumberová, Veronika (advisor)
The diploma thesis deals with evaluation of the current state of the Vinotéka Rek family business and results in a strategy of its further development. The first theoretical part is based on the theoretical backgrounds focusing on the development and growth of entrepreneurship in particular small and family businesses, the context of strategic management within the development of companies and also on selected analytical and research methods that examine the internal, external and market environment of the company. The second, analytical part of the thesis contains the practical application of the methods to the selected company, the identification of key factors and impacts on the company and the evaluation of the overall results for the subsequent selection of the appropriate development variant. Third, the part of proposals builds on the results of the analyzes and processes the selected variant of the development strategy, including the implementation steps and the benefits of the proposals.
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True random number generator
Procházková, Lenka ; Burda, Karel (referee) ; Člupek, Vlastimil (advisor)
The goal of this bachelor thesis is to apprise with true random number generators and their realisation. At first, random numbers and true random number generators are described and principles, details and properties are explained. The thesis also describes the use of generators in computers and in IoT devices and random sequences testing. The part of this thesis is description and realisation of computer generator and description of smartphone generator. The source of entropy used in this work is cursor move. Generated sequences were tested by the NIST STS and compared with sequences from other generators.
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Extremophilic microorganisms
Procházková, Lenka ; Vítová, Eva (referee) ; Babák, Libor (advisor)
This bachelor’s thesis deals with the extremophilic microorganisms. There is described extreme environment in which these microorganisms occur, as well as several structural and chemical adaptations to the environment. In most cases, adaptation to extreme environment requires changes in the composition of membrane lipids, nucleic acids and proteins. Enzymes with stability in extreme environment are used in chemical, food, pharmaceutical and textile industry. There is also mentioned the application of extremophiles in the biodegradation of organic materials. This bachelor’s thesis is mainly focused on hyperthermophilic microorganisms, which have been most researched.
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Verification of fermentative process of grape juice
Procházková, Lenka ; Nováková, Olga (referee) ; Vránová, Dana (advisor)
This thesis deals with identification of yeasts isolated during spontaneous fermentation of grape juice. For analysis the Pinot Noir grape variety grown in the integrated vineyard was chosen. In the theoretical part of this thesis basic information about yeasts are described. Genera of yeasts that occurs during fermentation process and methods based on PCR are also described. In this thesis PCR-RFLP method was used for identification of yeasts. The amplification of the 5,8S-ITS rDNA sequence was performed by the polymerase chain reaction with use of the primers ITS1 and ITS4. The restriction analysis was performed by applying five restriction endonucleases: HaeIII, HinfI, Taq?, AluI, MseI. The amplicons were split into fragments which length and number are typical for the particular species. These fragments were identified by agarose gel electrophoresis and electrophoreograms were evaluated by BioNumerics software. Dendrograms representing genetic similarity of isolated wine yeasts were created by using UPGMA cluster analysis.
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The use of dynamic non-destructive methods for determining the strength of cement from cement plant Hranice
Procházková, Lenka ; Novosad, Petr (referee) ; Brožovský, Jiří (advisor)
Nondestructive testing methods are employed as a tool for evaluation of parameters of building materials, especially to ascertain strength properties and to determine alteration occured during durability tests. An ultrasonic pulse velocity method is one of the most used nondestructive methods. A resonance method is employed among the nondestructive methods less. The aim of this work is to establish the calibration relationships between parameters of nondestructive testing and compressive strength value and flexural tensile strength value, respectively. Moreover, applicability of the calibration relationships is also discussed.
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Exploring the diversity of snow algae using polyphasic approach
Procházková, Lenka ; Nedbalová, Linda (advisor) ; Karsten, Ulf (referee) ; Hoham, Ronald (referee)
Snow algae cause blooms in slowly melting snowfields in mountain and polar regions. Although they are excellent models for the study of life in extremely cold environments, their taxonomical diversity, geographical distribution and variety of physiological strategies used to cope with their harsh environment are only partially understood. This work was focused on green algae from the order Chlamydomonadales and in one case on golden algae from the order Hibberdiales. An integrative approach was applied to characterise species, including the sequencing of several molecular markers (18S rDNA, ITS2 rDNA, rbcL) to reveal genotypes and infer phylogenetic positions. Light and electron microscopy were conducted to describe the detailed structure of cell wall surfaces and intracellular compartments. Moreover, fatty acid and pigment profiling were carried out to provide new insights into the adjustments of metabolic pathways in these algae. Rapid light curve measurements were used as a proxy of light preferences of photosystem II. Firstly, one of the main algae responsible for causing the phenomenon of red snow was shown to represent a single, monophyletic lineage, independent from other algae within the Chlamydomonadales (paper I). Therefore, the new genus Sanguina (S.) was described, with two...
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