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Ranking of Silver Mining Companies for Hedge Fund Management
Lišková, Petra ; Ptáček, Roman (referee) ; Rejnuš, Oldřich (advisor)
This bachelor thesis deals with an analysis of actual status of silver mining companies in order to rank these companies on the basis of one selected criterion. The subject of this thesis is also a sectoral analysis of silver mining industry and an analysis of individual pre-selected companies which deal with silver mining and meet the requirements set by the hedge fund management. This analysis will include an evaluation of their business indicators. An investment proposal for management of hedge fund will be made based on the evaluation of analyses and a comparison of selected companies.
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Molecular genetic analysis of rare ocular disorders in Roma population from the Czech Republic
Rysková, Natálie ; Lišková, Petra (advisor) ; Klímová, Aneta (referee)
The Roma are the largest and most widespread transnational ethnic community. The Czech Republic estimates their 2% representation in its population. Due to the high level of endogamy, the spectrum and frequency of hereditary diseases in the Roma population differ from the majority population. Hereditary eye diseases are one of the most common causes of blindness in younger adults and thus represent a real socio-economic burden. The aim of the thesis was to perform molecular genetic analysis in individuals of Roma origin suffering from hereditary diseases affecting vision, including dual impairments and syndromes, and to determinate the frequency of the detected pathogenic variants in this population. Molecular genetic analysis of 17 families was performed using direct and whole exome sequencing. Within the framework of an international collaboration, the degree of their mutual kinship was calculated using the PLINK program. The frequency of selected variants was determined in a control dataset comprising 156 Roma exomes and genomes. The spectrum of analyzed diseases included various retinal dystrophies, primary congenital glaucoma, Usher syndrome, neuronal ceroid lipofuscinosis, Noonan syndrome, nanophthalmos and congenital cataract, facial dysmorphism and neuropathy. The results of the thesis...
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Identification of type III secretion effectors in Aeromonas spp.
Jirsová, Anežka ; Kamanová, Jana (advisor) ; Lišková, Petra (referee)
Bacteria of the genus Aeromonas are Gram-negative, rod-shaped bacteria that are ubiquitous in the aquatic environment. The mesophilic species of this genus, represented by A. hydrophila, A. dhakensis, A. veronii and A. schubertii, are not only important pathogens in fish but also emerging pathogens in humans. They are associated with various gastrointestinal, systemic and wound infections. Their virulence has been described as multifactorial and associated with the presence of a type 3 secretion system. However, not much is known about the effector proteins that are introduced into the host cell by this system. The aim of this work was to characterize the two different type 3 secretion systems API-1 and API-2 of A. schubertii with respect to their cellular cytotoxicity and to identify the effector proteins that they transport into the host cell. To achieve these goals, a variety of methods were used, including microbiological techniques, molecular cloning, tissue culture techniques, SDS polyacrylamide gel electrophoresis, and mass spectrometry. Successful construction of mutant strains of A. schubertii with inactivated API-1 or API-2 and a strain with deletion of the gene encoding the negative regulator of API-1 enabled me to identify potential type 3 effector proteins of this species. Among the...
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Ranking of Silver Mining Companies for Hedge Fund Management
Lišková, Petra ; Ptáček, Roman (referee) ; Rejnuš, Oldřich (advisor)
This bachelor thesis deals with an analysis of actual status of silver mining companies in order to rank these companies on the basis of one selected criterion. The subject of this thesis is also a sectoral analysis of silver mining industry and an analysis of individual pre-selected companies which deal with silver mining and meet the requirements set by the hedge fund management. This analysis will include an evaluation of their business indicators. An investment proposal for management of hedge fund will be made based on the evaluation of analyses and a comparison of selected companies.
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Microbial communities of soils affected by long-term heavy metal contamination
Průchová, Pavla ; Kopecký, Jan (advisor) ; Matyska Lišková, Petra (referee)
This work is focused on microbial communities living in the soil affected by long-term exposure to heavy metals. The soil was sampled at two sites with different levels of contamination near Příbram. In the samples, respiration rate was measured in vitro after addition of carbon sources and at different levels of cadmium, one of the contaminating metals found in the soil. After the incubation with carbon sources, soil samples were collected for environmental DNA isolation. Gene coding for 16S rRNA in Actinobacteria was amplified from the environmental DNA samples and the amplicon composition was assessed by terminal restriction fragment length polymorphism analysis. The resulting profiles were used to compare actinobacterial communities in both groups of soil samples and in individual treatments. The analysis showed a clear distinction between the two sites differing in the contamination level and shifts in the community composition of various intesity depending on the added substrate.
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Structure and evolution of efflux pump in gramnegative bacteria.
Gálová, Diana ; Lichá, Irena (advisor) ; Matyska Lišková, Petra (referee)
Drug resistence in microorganisms is of major concern and there is an increasing number of pathogenic bacteria resistent to clinically used antibiotics. The major mechanism of resistence in Gram-negative is active efflux that prevent the intracellular accumulation of antibiotics to toxic levels. We recognise five families of efflux pumps, mainly using proton motive force to translocate the substrate, less common driven by ATP hydrolysis. The multidrug pumps with broad substrate specifity are of a key role in drug resistance of pathogens, typically represented by RND superfamily. The source of efflux pumps are probably the producers of antibiotics and organisms exposed to toxic compounds in their natural habitat. The genes for resistance can then spread out via horizontal gene transfer to other bacteria. It is presumed that eflux pumps have developed from transporters serving physiological functions like transport of endogenous substrates.
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Biofilm formation in Streptococcus pneumoniae
Jarošová, Václava ; Petráčková, Denisa (advisor) ; Matyska Lišková, Petra (referee)
Biofilm is a structured community of cells adhered to the surface or to each other and surrounded by extracellular matrix. Biofilm is fomed in several steps starting from single cells adhered to the surface up to microcolonies linked by channels. Because of a higher resistance to antibiotics the current hot topics in the biofilm research are formation of biofilms on medical materials and treatment of bacterial infections associated with biofilms. This work is focused on a biofilm forming bacteria Streptococcus pneumoniae. It is a potentially pathogenic bacterium which colonizes the upper respiratory tract and causes a number of diseases. Biofilms formed by S. pneumoniae exhibit increased resistance to antibiotics, therefore, alternative antimicrobial agents have been recently studied. For biofilm formation studies both open and closed systems are used. The flow cell and biofilm reactors represent commonly used open systems. Closed cultivation systems are for example a Calgary biofilm device and a micro titer plate-system developed by Christensen et al.
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Bacterial RTX toxins and their calcium-binding sites
Lišková, Petra ; Konopásek, Ivo (advisor) ; Holoubek, Aleš (referee) ; Hof, Martin (referee)
FrpC protein produced by Neisseria meningitidis in a human host belongs to the family of bacterial RTX toxins due to the presence of RTX domain. FrpC possesses a calcium-dependent auto-catalytic cleavage activity which is localized within its 177 amino-acids long segment Self-Processing Module (SPM). As the SPM is naturally intrinsically disordered protein without bound Ca2+, the calcium binding is crucial for SPM folding which is followed by the auto-catalytic processing. The elucidation of the SPM structure may be the key step for understanding of enzymatic and biological function. The structure of folded SPM itself can be characterized only with difficulties due to the presence of flexible loop according to preliminary NMR data. The subject of this work is the description of SPM using fluorescence methods, characterization of ions binding to SPM and structural changes occurring during Ca2+ binding. In this work, the ion binding properties of SPM segment and its ion-induced folding was characterized. It was found that the dissociation constant kD of 17 μM coincided with the folding of SPM into the native calcium-bound state which occurs in the concentration range between 1 and 20 μM Ca2+. In the attempt to characterize the structure of ion binding site, the fully active single tryptophan mutants...
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Viral and cellular RNA-dependent RNA polymerases
Krupicová, Daniela ; Španielová, Hana (advisor) ; Lišková, Petra (referee)
Viral and cellular RNA dependent RNA polymerases Krupicová Daniela Abstract RNA dependent RNA polymerases synthesize RNA strand from RNA template. They can be divided into two large groups - viral (RdRp) and cellular (RDR) polymerases. Viral RdRp play a key role in the life cycle of RNA viruses, ensuring replication and transcription of their genome. The structure, which resembles the "right hand", is similar to other types single-subunit polymerases. Large amounts of knowledge are available about the RdRp of RNA viruses with positive single strand RNA genome. This information then helps in the development of antiviral drugs targeting the RdRp. The second group consists of cellular RDR. These polymerases are found only in eukaryotes. Their active site is homologous to the active site of multisubunits DNA dependent RNA polymerases. RDR is involved in RNA silencing pathways.
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