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Functional characterisation of new components of mitochondrial proteome.
Kovalčíková, Jana ; Vrbacký, Marek (advisor) ; Červinková, Zuzana (referee) ; Ješina, Pavel (referee)
1 Abstract It has been estimated that the mammalian mitochondrial proteome consists of ~1500 distinct proteins and approximately one quarter of them is still not fully characterized. One of these proteins is TMEM70, protein involved in the biogenesis of the eukaryotic F1Fo-ATP synthase. TMEM70 mutations cause isolated deficiency of ATP synthase often resulting in a fatal neonatal mitochondrial encephalocardiomyopathies in patients. To understand the molecular mechanism of TMEM70 action, we generated constitutive Tmem70 knockout mice, which led to embryonic lethal phenotype with disturbed ATP synthase biogenesis. Subsequently generated inducible Tmem70 mouse knockout was lethal by the week 8 post induction. It exhibited primarily impaired liver function, which contrasts with the predominantly cardiologic phenotype at disease onset in humans. Liver mitochondria revealed formation of labile ATP synthase subcomplexes lacking subunit c. Thus, in case of TMEM70 deficiency c-oligomer was not incorporated into ATP synthase, which led to critical impairment of mitochondrial energy provision, analogous to TMEM70 dysfunction in humans. In TMEM70 deficient models, the ATP synthase deficiency reached the 'threshold' for its pathologic presentation, which we quantified at 30 %. We observed compensatory increases in the...
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Nursing care for the patient after alcohol septal ablation for hypertrophic obstructive cardiomyopathy
Kovalčíková, Jana ; Zvoníčková, Marie (advisor) ; Zemánek, David (referee)
Cílem mé práce je zpracování případové studie ošetřovatelské péče o pacientku G. E. (56 let), která byla přijata s diagnózou hypertrofické obstrukční kardiomyopatie k provedení alkoholové septální myokardiální ablace. V klinické části popisuji onemocnění - jeho příznaky, diagnostiku, terapii, včetně léčebného výkonu a rehabilitace. Dále se zabývám průběhem hospitalizace (příjem, vyšetření, invazivní výkon, farmakoterapie) paní G. E. V ošetřovatelské části se zabývám ošetřovatelským procesem podle M. Gordonové (anamnéza, aktuální a potenciální diagnózy, plán a realizace ošetřovatelské péče, hodnocení, dlouhodobý plán). K této části jsou připojeny informace o edukaci, psychologická a sociální oblast.
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Functional characterisation of new components of mitochondrial proteome.
Kovalčíková, Jana ; Vrbacký, Marek (advisor) ; Červinková, Zuzana (referee) ; Ješina, Pavel (referee)
1 Abstract It has been estimated that the mammalian mitochondrial proteome consists of ~1500 distinct proteins and approximately one quarter of them is still not fully characterized. One of these proteins is TMEM70, protein involved in the biogenesis of the eukaryotic F1Fo-ATP synthase. TMEM70 mutations cause isolated deficiency of ATP synthase often resulting in a fatal neonatal mitochondrial encephalocardiomyopathies in patients. To understand the molecular mechanism of TMEM70 action, we generated constitutive Tmem70 knockout mice, which led to embryonic lethal phenotype with disturbed ATP synthase biogenesis. Subsequently generated inducible Tmem70 mouse knockout was lethal by the week 8 post induction. It exhibited primarily impaired liver function, which contrasts with the predominantly cardiologic phenotype at disease onset in humans. Liver mitochondria revealed formation of labile ATP synthase subcomplexes lacking subunit c. Thus, in case of TMEM70 deficiency c-oligomer was not incorporated into ATP synthase, which led to critical impairment of mitochondrial energy provision, analogous to TMEM70 dysfunction in humans. In TMEM70 deficient models, the ATP synthase deficiency reached the 'threshold' for its pathologic presentation, which we quantified at 30 %. We observed compensatory increases in the...
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Export of biomolecules from mitochondria
Pelc, Josef ; Doležal, Pavel (advisor) ; Kovalčíková, Jana (referee)
Mitochondrion is cellular organelle evolved from the endosymbiotic bacteria. Because of its bacterial origin mitochondrion has a number of unique properties and metabolic pathways. Mitochondria or its reduced forms are present in all eukaryotic organisms except a single exception. During the course of evolution most of mitochondrial DNA was transferred to the nucleus. Despite this, mitochondria still encode several proteins that are part of the electron transport chain and the ATP synthase. These proteins are transported from the mitochondrial matrix into the inner mitochondrial membrane. Misfolded proteins of the inner membrane are degraded by mitochondrial proteases, which leads to the release of generated peptides. Fe-S clusters are assembled in the mitochondrial matrix. Still unknown molecule, which is product of ISC pathway is transported into cytosol and participates in the assembly of cytosolic and nuclear iron-sulfur proteins. Other transport pathway ensures the exchange of lipids between the endoplasmic reticulum and mitochondria. Both mitochondrial membranes have different lipid composition. To maintain their properties specific lipid transport is required between the two mitochondrial membranes. This transport pathway is also used for the lipid synthesis in the inner mitochondrial membrane.
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Mitochondrial subpopulations in rat myocardium - effect of chronic hypoxia
Kovalčíková, Jana ; Nováková, Olga (advisor) ; Žurmanová, Jitka (referee)
Adaptation to chronic hypoxia induces endogenous cardioprotection and increases the heart resistance to ischemia/reperfusion injury. The heart mitochondria, which produce reactive oxygen species (ROS) in addition to ATP, play an important role in these processes. During ischemia/reperfusion, ROS are produced in excessive amounts and damage the cells. However, in lower concentrations, ROS are involved in the signalling pathway of cardioprotection induced by adaptation to chronic hypoxia. In the heart, two mitochondrial subpopulations have been observed, subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria (IMFM), which differ in cell localization as well as in morphological and biochemical properties. The aim of this work was to introduce the method of SSM and IMFM isolation in our laboratory and to analyse their antioxidative capacity after adaptation to chronic hypoxia. Adult male Wistar rats were kept either under normoxic conditions or exposed to intermittent high-altitude hypoxia (IHA; 7000 m, 5 days a week/8 hours a day, totally 25 exposures). Mitochondrial subpopulations were isolated from heart left ventricle and their functionality was verified by measuring oxygen consumption and enzyme activities. The IMFM had higher oxygen consumption in comparison with SSM and activities...
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Nursing care for the patient after alcohol septal ablation for hypertrophic obstructive cardiomyopathy
Kovalčíková, Jana ; Zvoníčková, Marie (advisor) ; Zemánek, David (referee)
Cílem mé práce je zpracování případové studie ošetřovatelské péče o pacientku G. E. (56 let), která byla přijata s diagnózou hypertrofické obstrukční kardiomyopatie k provedení alkoholové septální myokardiální ablace. V klinické části popisuji onemocnění - jeho příznaky, diagnostiku, terapii, včetně léčebného výkonu a rehabilitace. Dále se zabývám průběhem hospitalizace (příjem, vyšetření, invazivní výkon, farmakoterapie) paní G. E. V ošetřovatelské části se zabývám ošetřovatelským procesem podle M. Gordonové (anamnéza, aktuální a potenciální diagnózy, plán a realizace ošetřovatelské péče, hodnocení, dlouhodobý plán). K této části jsou připojeny informace o edukaci, psychologická a sociální oblast.
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The role of FAT/CD36 trasporter in the pathophysiology of heart
Kovalčíková, Jana ; Nováková, Olga (advisor) ; Wasková, Petra (referee)
1. Abstract FAT/CD36 is an 88 kDa glycoprotein that plays a key role in the transport of long- chain fatty acids (LCFA) through the plasma membrane in heart, skeletal muscle and adipose tissue. It participates in the fatty acids (FA) transport together with other membrane proteins, which are fatty acid transport proteins (FATP1-6), the plasma membrane fatty acid binding protein (FABPpm) and cytosolic fatty acid binding protein (FABPc). In cardiac tissue are, except FAT/CD36, only represented FABPpm, FATP1 and 6 and heart type of FABPc, referred to as H-FABPc. In addition to this protein mediated FA transport, FA are already known to be transported by passive diffusion. The cell expression of FAT/CD36 is regulated by nuclear peroxisome proliferators- activated receptor (PPAR), in the heart primarily by PPAR- . Inactive FAT/CD36 is found in intracellular depots, while active FAT/CD36 is present on the plasma membrane in lipid rafts. The two most known pathways regulating the FAT/CD36 translocation from the depots to the membrane are the insulin signalling pathway, which involves the activation of the enzyme phosphatidyl-inositol-3-kinase (PI3K) and cardiac contraction activated cascade, which activates adenosinmonophosphate kinase (AMP kinase). Furthermore, FAT/CD36 can as well be regulated by ubiquitination...
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One and one is two... or one?
Kovalčíková, Jana ; Šiktancová, Jaroslava (advisor) ; Vinař, Josef (referee)
To seek or to find?
To ask or to answer questions?
To rehearse or to perform a play?
To be an actress or to want to be an actress?
Which is the more important level of being, of approach?
One should be inseparably linked with the other. One little particle formed by dozens others.
Individuality of an individual illustrated in an image reflected in the mirror. In the first part it is a broken mirror, in the second part a fragment of mirror picked up from the ground, in the third part the broken mirrror put back together, which is neither better or worse - it is simply different.
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