|
|
Current methods in protein-protein interactions research
Křivková, Jana ; Hrdý, Ivan (advisor) ; Kučerová, Jitka (referee)
Protein-protein interactions (PPI) have a crucial role in all processes in living cells. Understanding the interactions between proteins allows us to describe cell processes in more detail and their study opens new possibilities for drug design. The importance of the question of studying PPI is shown in the recent development of various methods for their identification and description. The aim of this thesis is to give an overview of new and improved experimental methods of identification and characterization of protein-protein interactions. Methods described in this thesis are divided in four chapters - proximity-dependent labelling methods (BioID, BioID2, APEX, TurboID, MiniTurbo, PUP-IT, AirID, SPPLAT, EMARS), cross-linking methods (XS-MS), fluorescence methods for identification and visualization (BiFC, FRET, BRET) and biophysical methods for description of kinetics and thermodynamics parameters of interaction (SPR, ITC, MT).
|
|
|
Quality of patients life with celiac disease
Bičíková, Michaela ; Křivková, Jana (advisor) ; Vlachová, Marie (referee)
This thesis is dealing with the quality of life of patients with celiac disease. In the theoretical part, I am presenting the characteristics of celiac disease and I am describing its history briefly. For better orientation in the issue, I also joined a description of the anatomy of the small intestine and pathophysiology of celiac disease. In this thesis I am also dealing with incidence of disease, etiology and risk factors participating in the development of celiac disease, symptoms and the different forms, which are related to celiac disease, as well as potential complications and prognosis of celiac disease. An irreplaceable is the role of nurses in diagnosis and targeted screening for celiac disease, therapy and prevention, dispensarization and education of patients with celiac disease about gluten-free diet. I am also describing the problems that patients with celiac disease may encounter in everyday life. Last but not least I am focusing on the general concept of quality of life and also on the quality of life related to health and celiac disease. In the practical part, I am presenting aims and operational hypothesis, methodology of thesis and the characteristics of surveyed population sample. Chapter called the analysis of results includes pie charts showing the questionnaire's items,...
|
|
|
Impact of intestinal stoma of daily activities
Kougl, Jiří ; Nikodemová, Hana (advisor) ; Křivková, Jana (referee)
In the theoretical part I am devoted to the history, anatomy, types of stoma, concept of stoma, indications for an establishment of stoma, pre and postoperative care, treatment of stoma, instruments and the influence on daily activities of a client while having a stoma. In the practical part I am following my own research of the impact of stoma on daily life's activities of klients with a stoma.
|
|
|
Effect Multiple Sclerosis on activities of daily living
Němečková, Jana ; Nikodemová, Hana (advisor) ; Křivková, Jana (referee)
Bibliografický záznam NĚMEČKOVÁ, Jana. Vliv roztroušené sklerózy na aktivity denního života. Praha: Univerzita Karlova, 2. Lékařská fakulta, Ústav ošetřovatelství, 2016. 87 s., přílohy. Vedoucí bakalářské práce PhDr. Hana Nikodemová Abstract In my Bachelor thesis I examine the impact of Multiple sclerosis on a daily life activities. The intention is to present an overview of knowledge of the disease itself and map its impact on the activities of a normal day. The work is divided into theoretical and practical part. The introduction of the theoretical part is devoted to reasons of my decision to describe this topic, I define the aim of the work and the working hypotheses, subsequently there is described the disease itself in tabular form by each areas, such as the characteristics of the disease, duration of disease, diagnosis, treatment etc. Then I pay attention to daily life activities and needs of the patient. In the practical part is performed quantitative research through a questionnaire survey of patients from a specialized center for the treatment of demyelinating diseases in the Department of Neurology at the Motol University Hospital. The obtained data are presented in tabular and graphical reports. Keywords multiple sclerosis , activities of daily living, the impact of disease, attack, human needs
|
|
| |
|
|
Attitude of nurses to euthanasia
Konečná, Petra ; Křivková, Jana (advisor) ; Jirkovský, Daniel (referee)
The bachelor thesis "Attitude of nurses to euthanasia" focuses on an understanding of dying, euthanasia and palliative care and their attitude to euthanasia. The work is devided into two parts - a theoretical one and an empirical one. The theoretical part describes the methodology and results of quantitative research by the use of questionaire comparing two specified groups of nurses - hospic care nurses and intensive care nurses. There are summarised findings from the investigation in the conclusion which showes the differences between intensive care nurses and hospic care nurses in attitude to euthanasia and its legislative security, but not in the knowledge of palliative care.
|
|
|
The role of nurses in the application of low molecular weight heparin
Kubátová, Lenka ; Křivková, Jana (advisor) ; Tomová, Šárka (referee)
The bachelor thesis "Role of a nurse in application of a low-molecular-weight heparin" focuses on the topic of application of a low-molecular-weight heparin. The aim of the research was to explore the correct technique of a subcutaneous application of a low- molecular-weight heparin and assess the role of nurses in educating patients about the application. The theoretical section provides a basic overview of the low-molecular-weight heparin and explains the principles of antithrombotic therapy. The research section was implemented using a quantitative research technique, interviews using a structured questionnaire. The survey included 130 respondents working at standard surgical, orthopaedic, internal medicine wards and intensive care unit in the University Hospital Motol. The results showed nursing problems in the technique of application of low-molecular-weight heparin. The same number of respondents report a correct application angle (62 %) and do not massage the injection site (63 %). One third of respondents remove air bubbles from a syringe, only 58 % of respondents keep the skin pinch throughout the application. The survey proved that nurses from standard wards and nurses with a University degree apply the correct technique of a low-molecular-weight heparin application better than nurses...
|
|
|
Impact of Pain in Patients with Hemophilia on their Emotional Experience and Quality of Life
Půčková, Romana ; Raudenská, Jaroslava (advisor) ; Křivková, Jana (referee)
The Bachelor's thesis deals with the issue of the impact of pain on emotional experience and quality of life in haemophiliac patients and the significance of pain management for nursing practice. The aim of the thesis was to tackle problems concerning the disease in adult haemophiliacs in connection with pain, emotional experience and quality of life, describe relations between these variables and determine the main factors influencing pain and quality of life in haemophiliacs. For that purpose, nine basic hypotheses were set and verified by the means of statistical processing of data obtained from a questionnaire survey of 105 adult haemophiliacs of age among 18 to 79 addressed across the whole Czech Republic. The respondents represented patients of both, A and B haemophilia and of all kinds of haemophilia severity. Four instruments were used to carry out the survey - a standardized questionnaire A36 Hemofilia-QoL for determining quality of life, a standardized questionnaire BDI - II for determining depressive symptoms, a standardized questionnaire GAD 7 for determining the level of anxiety and a visual-analogical scale, so-called Thermometer Scale of Emotions. The survey included a semi- structured questionnaire for demographic-anamnesis data. By the means of the instruments mentioned above,...
|
|
|
Nurses' knowledge of electrocardiography
Fialová, Iveta ; Křivková, Jana (advisor) ; Nikodemová, Hana (referee)
The bachelor thesis deals with the general knowledge of nurses in the area of electrocardiography, hence also its name Knowledge of nurses about electrocardiography. In the theoretical part, electrography, its history, recordings, pathological rhythms and nursing care associated with rhythm detection are described. In the empirical part, the results of quantitative research carried out by the questionnaire are interpreted over the methodology. At the end of the thesis are summarized the results of the questionnaire survey, which shows shortcomings in education during preparation for the general nurse's profession. These deficiencies are then manifested by insufficient knowledge of the recognition of basic pathological ECG rhythms, eventual defibrillation of some pathological rhythms. The lowest knowledge in this area has nurses with secondary education only. From the questionnaire survey, it is clear that nurses would appreciate not only more tuition during their general nurse training, but would also take part in electrocardiography workshops.
|
|
|
Meeting the needs of patients with chronic obstructive pulmonary disease
Čejková, Denisa ; Tomová, Šárka (advisor) ; Křivková, Jana (referee)
The intention of the thesis was based on an exploratory investigation to determine whether the needs of patients with COPD varies depending on the stage of the disease. The work is divided into theoretical and empirical part. The theoretical part is divided into three chapters. The first slew characteristic of COPD, its diagnosis and subsequent treatment. The second deals with the classification of needs, motives and consequences that arise for patients insufficient meet the needs. The third part focuses on the specifics of treating patients with COPD. In the empirical part presents the results of quantitative research, which was conducted via questionnaire. Questionnaires were distributed to hospitalized patients with chronic obstructive pulmonary disease in stage II, III and IV on the second clinic of Pneumology, University Hospital Motol. Exploratory survey was conducted anonymously, from July to December 2017. The main criterion for selection of respondents were diagnosed with COPD, good physical condition, mental well-being and willingness of patients to share their feelings. A total of 76 patients were approached, of which three savings would not participate. From the exploratory investigation showed that patients II. stages are distinctly identified needs, than patients in stage III and IV....
|
guest ::
RSS feed.