|
|
The repair of DNA damage induced with suphur mustard and its relationship to the cytotoxicity
Jílková, Martina ; Vopršalová, Marie (advisor) ; Herink, Josef (referee)
The aim of this study was to investigate the induction and repair mechanisms of DNA damage caused by sulphur mustard in specific cell lines and their relationship to cytotoxicity of sulphur mustard. Sulphur mustard is a chemical warfare agent of the blistering agent category, which can be misused in local conflicts, terrorist attacks and during liquidation of its storage. Sulphur mustard is an alkylating agent, which interacts with a wide range of cellular macromolecules including DNA, RNA and proteins. Sulphur mustard forms single - strand breaks, monofunctional guanine and adenine adducts, as well as interstrand cross-links involving the two guanines in interaction with DNA. This study is aimed at cross-links. We used the Single Cell Gel Electrophoresis (SCGE, comet assay) for their detection. It is a method of evaluation of single cells ambeded in agarose. It is used for detection of DNA single strand breaks in a single cell. In our modification of the method we determined cross-links, which make the alkaline DNA unwinding impossible. That is why we had to induce a standard number of single strand breaks in DNA by styreneoxide (at a certain concentration and exposure time) before the comet assay. We studied DNA repair mechanisms of specific DNA lesions using specific cell lines with clearly...
|
|
|
Long term development of life expectancy in the world
Jílková, Martina ; Novotný, Josef (advisor) ; Pospíšilová, Lucie (referee)
Life expectancy has been rising since the end of the 18th century. This thesis aims to characterize its long term development with emphasis on the period after 1800, whereas the beginning periods of sustained growth and its dynamics are represented. Gains in survival began successively in individual countries, and it was found that the later the growth began, the more rapid it was. The determinants of mortality decline are set and described, as well. The attention is paid also to the relation between life expectancy and economic level. The analysis proved strong dependence of life expectancy on income in the course of the 20th century. Finally, the exceptions to this trend are discussed.
|
|
|
Conflict in the perception of architectural heritage qualities and consequences for urban regeneration: The case of Ostrov
Jílková, Martina ; Temelová, Jana (advisor) ; Šilhánková, Vladimíra (referee)
Over the last few decades Czech cities have experienced significant improvements of physical environment, and urban regeneration is one of the processes contributing to these changes. The final appearance of renewed environment is the result of negotiations and conflicts among key actors involved in the regeneration process. The decision- making process of urban regeneration is influenced by many factors including the perception of the qualities of the buildings and of the place. The regeneration process can also change the place and its meanings. The aim of this diploma thesis is to characterize the role of perception in decision-making process of urban regeneration in the case of two parts of the city of Ostrov built in different architectural styles. First, the theoretical knowledge related to perception of the architectural heritage and its regeneration is discussed, with emphasis on the concept of place as a pervading approach. As the main research findings, the differences in the perception of given buildings were identified among the city residents and among the actors as well. It was also proven that the perception of given buildings affects the approach of the actors to regeneration to some extent, although in many cases the other factors predominate.
|
|
|
Long term development of life expectancy in the world
Jílková, Martina ; Novotný, Josef (advisor) ; Pospíšilová, Lucie (referee)
Life expectancy has been rising since the end of the 18th century. This thesis aims to characterize its long term development with emphasis on the period after 1800, whereas the beginning periods of sustained growth and its dynamics are represented. Gains in survival began successively in individual countries, and it was found that the later the growth began, the more rapid it was. The determinants of mortality decline are set and described, as well. The attention is paid also to the relation between life expectancy and economic level. The analysis proved strong dependence of life expectancy on income in the course of the 20th century. Finally, the exceptions to this trend are discussed.
|
|
|
The repair of DNA damage induced with suphur mustard and its relationship to the cytotoxicity
Jílková, Martina ; Vopršalová, Marie (advisor) ; Herink, Josef (referee)
The aim of this study was to investigate the induction and repair mechanisms of DNA damage caused by sulphur mustard in specific cell lines and their relationship to cytotoxicity of sulphur mustard. Sulphur mustard is a chemical warfare agent of the blistering agent category, which can be misused in local conflicts, terrorist attacks and during liquidation of its storage. Sulphur mustard is an alkylating agent, which interacts with a wide range of cellular macromolecules including DNA, RNA and proteins. Sulphur mustard forms single - strand breaks, monofunctional guanine and adenine adducts, as well as interstrand cross-links involving the two guanines in interaction with DNA. This study is aimed at cross-links. We used the Single Cell Gel Electrophoresis (SCGE, comet assay) for their detection. It is a method of evaluation of single cells ambeded in agarose. It is used for detection of DNA single strand breaks in a single cell. In our modification of the method we determined cross-links, which make the alkaline DNA unwinding impossible. That is why we had to induce a standard number of single strand breaks in DNA by styreneoxide (at a certain concentration and exposure time) before the comet assay. We studied DNA repair mechanisms of specific DNA lesions using specific cell lines with clearly...
|
guest ::
RSS feed.