|
|
Methodology for assessing the effect of sublethal doses of pesticides on solitary bees employing the OMICs approach
Erban, Tomáš ; Shcherbachenko, Elena ; Šlachta, Martin ; Cudlín, Pavel ; Chalupníková, Julie ; Halešová, Taťána ; Tomešová, Daniela ; Václavíková, Marta ; Votavová, Alena
Pollinators are potentially exposed to a number of pesticides in the environment. The most important group of hazardous substances comes from the category of plant protection products (PPPs), which are applied not only by farmers but also by small gardeners. Each PPP including the active substance and formulation undergoes a very detailed and thorough evaluation, which is in the European Union (EU) based on Regulation (EC) No 1107/2009 of the European Parliament and of the Council. Although the negative effects of PPPs on the environment and non-target organisms are minimized with proper use, we cannot rule out possible hidden effects, especially those of a sublethal nature. Modern analytical approaches, such as the application of high-throughput methods, help to identify hidden side effects. This methodology is focused on the evaluation of the effect of sublethal doses of pesticides on solitary bees employing the OMICs approach. In the risk assessment of pesticides, the honey bee has long been preferred as a model species of pollinator, while solitary bees stay in the background. The methodology contains innovative aspects for experiments, such as a modified isolator, preparation of plants for exposure, the timing of the experiment, monitoring of active substance distribution or proteomic analysis of solitary bees to identify hidden effects of the test substance or its formulation. In this methodology, the model of the solitary bee, red mason bee (Osmia bicornis), is considered. However, the methodology is also applicable to other, especially related species of solitary bees. The methodology can be used in areas of state administration, private laboratories and research activities in the assessment of environmental risks of pesticides on solitary bees. The methodological procedure can confirm or even eliminate environmental risks when registering new products or reevaluating existing products. This methodology therefore has the potential for use in testing new substances intended for plant protection before their registration. In an exemplary embodiment, the effect of acetamiprid in a Careo sticks formulation was tested. The distribution of acetamiprid in the soil substrate and the plant was evaluated. The hazardous metabolite acetamiprid IM-2-1 was identified in plant tissues. However, proteomic analysis indicated a very low risk of acetamiprid for O. bicornis. This result is in agreement with the fact that acetamiprid has been assessed by EFSA to be a low risk for bees and is registered in the EU until 28 February 2033.
Fulltext:  PDF
|
|
|
Methodology for assessing the effect of sublethal doses of pesticides on solitary bees\nemploying the OMICs approach
Erban, T. ; Shcherbachenko, E. ; Šlachta, Martin ; Cudlín, Pavel ; Chalupníková, J. ; Halešová, T. ; Tomešová, D. ; Václavíková, M. ; Votavová, A.
Pollinators are potentially exposed to a number of pesticides in the environment. The\nmost important group of hazardous substances comes from the category of plant protection\nproducts (PPPs), which are applied not only by farmers but also by small gardeners. Each PPP\nincluding the active substance and formulation undergoes a very detailed and thorough evaluation,\nwhich is in the European Union (EU) based on Regulation (EC) No 1107/2009 of the European\nParliament and of the Council. Although the negative effects of PPPs on the environment and nontarget organisms are minimized with proper use, we cannot rule out possible hidden effects,\nespecially those of a sublethal nature. Modern analytical approaches, such as the application of\nhigh-throughput methods, help to identify hidden side effects. This methodology is focused on the\nevaluation of the effect of sublethal doses of pesticides on solitary bees employing the OMICs\napproach. In the risk assessment of pesticides, the honey bee has long been preferred as a model\nspecies of pollinator, while solitary bees stay in the background. The methodology contains\ninnovative aspects for experiments, such as a modified isolator, preparation of plants for exposure,\nthe timing of the experiment, monitoring of active substance distribution or proteomic analysis of\nsolitary bees to identify hidden effects of the test substance or its formulation. In this methodology,\nthe model of the solitary bee, red mason bee (Osmia bicornis), is considered. However, the\nmethodology is also applicable to other, especially related species of solitary bees. The\nmethodology can be used in areas of state administration, private laboratories and research\nactivities in the assessment of environmental risks of pesticides on solitary bees. The\nmethodological procedure can confirm or even eliminate environmental risks when registering new\nproducts or reevaluating existing products. This methodology therefore has the potential for use in\ntesting new substances intended for plant protection before their registration. In an exemplary\nembodiment, the effect of acetamiprid in a Careo sticks formulation was tested. The distribution of\nacetamiprid in the soil substrate and the plant was evaluated. The hazardous metabolite\nacetamiprid IM-2-1 was identified in plant tissues. However, proteomic analysis indicated a very\nlow risk of acetamiprid for O. bicornis. This result is in agreement with the fact that acetamiprid\nhas been assessed by EFSA to be a low risk for bees and is registered in the EU until 28 February\n2033.
|
|
|
Effect of metapopulation structure and environmental factors on population biology of Impatiens noli-tangere infected by powdery mildew Podosphaera balsaminae
Chalupníková, Jana ; Koubek, Tomáš (advisor) ; Čuda, Jan (referee)
Studies performed on natural pathosystems revealed that the spatial structure of the metapopulation plays an important role in the development and spread of disease; phytopathological experimental studies have also revealed a significant effect of climatic factors on the presence of the disease (incidence) and the level of infection (prevalence) in populations. The aim of this study was to explore a natural pathosystem from both sides at the same time and to see which local and spatial environmental factors influence the development of pathosystem. Study focuses on population biology of natural pathosystem Podospaera balsaminae - Impatiens noli-tangere, which has not been investigated yet. In the study area (between the city Sázava (Benešov district) and village Vlkančice) host populations occurred in 78 (64 of them were infected) in 2013 and 82 populations (61 of them were infected) in 2014. Size of host populations had the strongest effect to incidence and prevalence of disease in the pathosystem. In larger populations, the disease occurred more often, developed faster and was higher. The rate of spread of the pathogen territory and the rate of population host connectivity had also strong effect. The abundance of pathogen in the studied area and the level of connectivity of host populations also...
|
|
|
Wheat streak mosaic virus: an important disease in cereal crops
Kumar, Jiban ; Slavíková, Lucie ; Chalupníková, Jana
Wheat streak mosaic virus (WSMV) causes serious disease in cereal crops, particularly in wheat. The main symptoms caused by WSMV include chlorotic streak mosaic on leaf, which become necrotic and gradually dry form the top of the leaf. In severe infection plant stunting and spike become sterile. WSMV is transmitted by cereal curl mite Aceria tosichella, and in low level (up to 1.5%) also transmitted by seeds. WSMV was detected in first time in the former Czechoslovakia in early eighties. The incidence of the virus in recent years is growing in the Czech Republic and even severe occurrence and wide distribution in some wheat fields in different regions of the country was determinated. This methodology put together recent knowledge of WSMV and its vector, including the incidence and possibilities of control cereal crops against the virus.
Fulltext: PDF
|
guest ::
RSS feed.