|
Practical Use of Data Mining Technologies
Uhlíř, Radek ; Pour, Jan (advisor) ; Zajíc, Ján (referee)
This bachelor's thesis maps available technologies of extracting knowledge from the raw data. These methods are globally known as Data Mining. Some of these methods are implemented in the second part - proof of concept of Data mining support in sales department. The aim of this work is to identify and implement suitable technologies for answering analytical questions and getting knowledge from data owned by business companies. It should help to improve and optimize business processes and resource utilization. Customer segmentation support and association rules identification are also expected. In the second part are identified possible weaknesses and problems during the process of implementation and deployment of these systems. The work should propose optimal methods of solving these problems or at least modifications in process of implementation to eliminate some vulnerability. The work is divided into two parts -- first is theoretical and maps available methods and second part is about implementation of project in pharmaceutical company. This solution was built using Microsoft SQL Server platform.
|
|
Analysis of pterine derivatives by capillary zone electrophoresis
Krajíček, Jan ; Bosáková, Zuzana (advisor) ; Čabala, Radomír (referee)
Pterins belong to an important group of compounds, acting as inhibitors, sensiziters, enzymes, coenzymes, pigments etc. and together with carotenoids and anthraquinones are responsible for characteristic coloration of bugs. This work was focused on the development of a capillary electrophoretic separation method for the analysis of six pterine derivatives, namely biopterine, neopterine, isoxanthopterine, leukopterine, xanthopterine and erythropterine and on their identification in the real samples. Separation was conducted in an uncoated fused-silica capillary termostated at 30 řC. Separation electrolyte contained boric acid, tris(hydroxymethyl)aminomethane and disodium salt of ethylenediaminetetraacetic acid. The effects of buffer pH, concentration of electrolyte components, separation voltage and wavelength of UV detection on electromigration behavior and detection sensitivity were studied. Under the optimized separation conditions, organic extracts of the three forms of Graphosoma semipunctatum bugs were analyzed.
|
|
Preparation of cochenial carmine
Helceletová, Zuzana ; Čapek Adamec, Martin (referee) ; Hrdličková Kučková, Štěpánka (advisor)
The aim of this study was to prepare a red organic pigment named cochineal carmine. The pigment was made out of dried insect called Coccus cacti using historical recipe. Next goal was to detect carminic acid by infrared spectroscopy with Fourier transform (FTIR) in dried bodies of the mentioned insect, then in the pigment prepared from it and in the pigment that was prepared using extract called carminum rubrum. The other results of this work were the comprehension of the origin and preparation of carmine pigment and the identification of carminic acid in both prepared pigments.
|
|
Changes of fatty acids during production of model cheese
Janoušková, Kateřina ; Babák, Libor (referee) ; Vítová, Eva (advisor)
This thesis deals with the identification and quantification of free and bound fatty acids in model cheese samples of the Moravský bochník type, which were produced at Tomas Bata University in Zlín by a standard technological procedure. In a theoretical part of the thesis, the issues of lipids, fatty acids, as well as the characteristics of high cooked cheeses including Moravský bochník, microbial cultures used in their production, and the possibilities of fatty acids determination are summarized. The ČSN EN ISO 1735:2005 method was chosen for the lipid extraction from cheese samples. Present free and bound fatty acids were identified and quantified by gas chromatography with flame-ionization detection after conversion to methylesters by acid esterification with bortrifluoride as a catalyst. In total 32 fatty acids were identified in cheese samples, the highest amount of caproic, caprylic, capric, lauric, myristic, palmitic, stearic and oleic acids.
|
|
Compounds of the Cichorium intybus extract
Málková, Marta ; Jahodář, Luděk (advisor) ; Siatka, Tomáš (referee)
Marta Málková: Compounds of the Cichorium intybus extract. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2008, 56 pp. Chloroform and ethanolic extracts, which were prepared by the addition of an original dry extract to solvents mentioned above, have been used for the identification of substances contained in Cichorium intibus L. seeds. The chloroform extract has been evaluated via the thin layer chromatography. β-sitosterol and β- amyrin have been detected by this procedure. The ethanolic extract has been separated by the column chromatography and obtained fractions have been evaluated by the thin layer chromatography to prove a presence of saccharides and phenolic acids. By this way saccharose has been detected. Then the sample has been analyzed by UPLC / MS and thus the presence of four isomers of isochlorogenic acid has been detected. .
|
|
Biological effect of plant metabolites III. Isolation of alkaloids from Eschscholzia californica Cham.
Linzerová, Petra ; Karlíčková, Jana (referee) ; Karlíčková, Jana (advisor)
Linzerová, Petra.: Biological Effect of Plant Metabolites III. Izolation of Alkaloids from Eschscholzia californica Cham. A Diploma Work, Charles University in Praque, Faculty Of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany And Ecology, Hradec Králové 2009, 54 pages Aims of this diploma thesis were to isolate alkaloids from J1 extract obtained from a herb with roots from a plant Eschscholzia californica Cham., to perform the identification of gained alkaloid and to participate in determination of activity of isolated alkaloid against the erythrocytic acetylcholinesterase. After finding suitable mix of reagents we divided the extract on a chromatografic column filled with silica gel by system of chloroform : ethanol with gradual increasing of concentration ethanol. With obtained eluents we made a orientation thin layer chromatography with developing system acetic acid : ethanol : water (60 : 30 : 10). Alkaloids were detected by UV radiation and spray with Dragendorff reagent. After decantation of fractions with similar spectrum of matters we cleared selected mixture. We obtained two alkaloids; NMR spectroscopy will be used to determine the structures of these alkaloids. Keywords: Eschscholzia californica Cham., benzo[c]phenantridin alkaloids, column chromatography, Alzheimer...
|
|
Alterations in protein expression induced by valproic acid in human T-lymphocyte cell line MOLT-4
Tichá, Zuzana ; Kvasničková, Eva (advisor) ; Řezáčová, Martina (referee)
Univerzita Karlova Farmaceutická fakulta Katedra biochemických věd EFFECT OF VALPROIC ACID (VA) ON CHANGES IN EXPRESSION OF PROTEINS OF HUMAN T-LYMPHOCYTE LEUKEMIA CELL LINE MOLT-4 Valproic acid (VA) is histondeacetylase inhibitor (HDACI) and it leads to inhibition in proliferation and apoptosis induction during incubation with leukemia cells. The aim of our article was to determine the changes in expression of proteins after incubation leukemia cells with VA. After 4-hours-long incubation of MOLT-4 cells with 2 and 4 mmol/l VA, the nuclear extracts from MOLT-4 cells were used to detection of changes in levels of proteins. The nuclear extracts were separated for first dimension-IEF in immobilized pH 4-7 gradient. Immediately after being focused, the proteins were separated for electophoretic way in the second dimension, 9-16% gradient DS was used. Proteins separated by 2D electrophoresis were visualized by silver staining , and silver-stained gels were digitized using a laser personal densitometer. Protein maps were quantified using a Melanie 3 software package. Proteins were identified by MALDI mass spectrometer. The finding protein process was finished by using ProFound and PeptIdent programs. Identification process of protein spots exhibiting significant abundance alterations induced by VA help to...
|
|
Postcranial skeleton of small mammals in biology education
Šmídová, Kateřina ; Mourek, Jan (referee) ; Řezníček, Jan (advisor)
This master thesis demonstrates feasibility of identification of small rodents in the pellets of Long-eared Owl {Asio otus) using pelvic bones and postcranial skeleton in biology classes. The first part of the text summarizes theoretical information on mammalian skeleton and owl pellets with in-class activities suggested. The methodology chapter describes methods of owl pellets collection and preparation, and subsequent processing of osteologic fragments extracted from the pellets. A list and relative distribution of small rodent species found during the analysis are included as well as a key for identification of small rodents using skeleton tragments. The outcome of the thesis are four teaching units that were successfully tested by the author on students from eight- and four-year Grammar Schools.
|
| |
|
Analysis of compounds contained in Acer platanoides L. flowers
Rajdlová, Stanislava ; Karlíčková, Jana (advisor) ; Macáková, Kateřina (referee)
SUMARY Extraction of compounds from flowers Acer platanoides was carried out in the first part of our work. Two extracts have been prepared - ether and ethanol. Identification of the compounds was done by the help of a thin layer chromatography with using the standards of phenolic compounds. According to the results TLC the presence of rutin, apigenin, hesperetine, hyperoside and chlorogenic acid were supposed. The presence of a compound of reducing character was proved in the ether extract. The HPLC analysis confirmed the presence of rutin in the ethanol extract. Further, the content of total phenolic compounds was determinated in the ethanol extract and results were expressed as gallic acid equivalent, i.e. 0,135 mg GAE/mg of extract. The second part of our work was aimed at testing antioxidant activity of ethanol extract by help of DPPH test. Very high values of antioxidant activity have been measured. Compounds of phenolic character were supposed to participate in antioxidant activity of ethanol extract.
|
guest ::
