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Comparison of methods of ECLIA and ELISA in determining the beta-CrossLaps
KOŽANTOVÁ, Jana
Osteoporosis is a very old disease affecting a large majority of population. Its occurrence rate has been growing permanently. It is a systemic disease of bones for which the decrease in bone mass, i.e. of inorganic as well as organic bone part is characteristic. Typical symptoms are osteoporotic fractures. To perform an in time diagnostics of the disease, it is necessary to execute the necessary examinations. One of them is also the laboratory determination of markers of bone resorption and new creation for finding out the state of bone metabolism. The target of my bachelor thesis was to find out the level of the bone resorption marker (beta-CrossLaps) by means of ELISA and ECLIA methods on the same serum from patients with the diagnosed osteoporosis and to process statistically the results. In the next part I dealt with the structure and functions of bones. Then I focused on markers of bone formation and resorption. Finally I mentioned the bone metabolism and its control mechanisms. I concentrated mainly on the metabolism of calcium, D vitamin, parathormone and calcitonin. I carried out the practical part of my bachelor's thesis in the accredited private laboratory STAFILA, spol. s r. o. in České Budějovice. The procedure of my work followed the standard operating procedure of the laboratory. In the research, in total 66 serums of patients with osteoporosis were analysed by me. At first, I determined the concentration by beta-CrossLaps method based on the principle of sandwich ELISA on the appliance NexGen Four from TestLine company. Then I analysed the same samples on the automatic analyser IDS-iSYS from IDS company, this time by ECLIA method. Both appliances on which the determination was executed, principles of both methods, applied analytic sets and the procedures of analysis are described in the methodical part. In the next part of my bachelor's thesis, the results of my measuring are stated. At first I entered the concentrations beta-CrossLaps in ng/ml measured by both methods into the table and for the respective samples I stated the sex and years of birth of individual patients. Moreover I prepared a graph illustrating the share of men and women of various age categories in my research. The second graph shows the differences in values of beta-CrossLaps concentrations between both methods. Resulting concentrations were subject to regression analysis. I applied linear regression and Grubbs test for finding out the outlying values excluded from the further statistical processing. I performed Passing-Bablok regression based on the remaining values. As a result of regression equation and 95% CI of the slope B and intercept A, the proportional error of measuring was rejected but on the contrary, the systematic error was confirmed. Correlation coefficient R = 0,97 shows a very good linear dependency between both methods. It turned out by practice in the laboratory that the evaluation of concentrations beta-CrossLaps from the same patients on various appliances may show partially different values. I worked at two different analysers, each of them was based on another principle, with a different sensitivity and calibration. All of this could have the influence on the resulting concentration of individual samples. The advantage of automatic analyser IDS-iSYS is a quicker determination. Moreover it is possible to perform on this apparatus all the possible examination concerning the bone metabolism from D vitamin, parathormone, osteocalcin up to bone markers. It is advantage for the patients with osteoporosis as well as with other metabolic skeleton diseases. It follows from this that all the necessary examinations may be carried out on one apparatus.
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Role of UNHCR in case of asylum seekers, refugees and stateless persons – the case study of Turkey
Unzeitigová, Klára ; Novotná, Markéta (advisor) ; Kuchařová, Alžběta (referee)
Inspired by the Kratochvíl, Cibulková and Beník´s model of actorness, the thesis explores the framing power of UNHCR. The critical discourse analysis introduced by Norman Fairclough is applied in order to analyze the textual, contextual and sociocultural dimension of the UNHCR framing power. The case of Turkey was chosen for this study due to the current situation and the fact that Turkey is one of the biggest hosting country in the refugees´ crisis. Through the critical discourse analysis, the thesis explores whether UNHCR is considered as a framing actor in the case of asylum seekers, refugees and stateless people in Turkey. The analysis shows that UNHCR is globally respected and takes important part in the issue. However, not all of the international organizations refer to UNHCR as the lawmaker and the initiator of debates in the issues mentioned above.
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International involvement in the peacebuilding process on Sri Lanka
Mojžíš, Michal ; Havlová, Radka (advisor) ; Burešová, Jana (referee)
The final months of Sri Lankan civil war, the 26 year military campaign between the Government of Sri Lanka and the insurgent separatist organization Liberation Tigers of Tamil Eelam, are marked by allegations of war crimes committed by both parties of the conflict, including attacks on civilians, summary executions of prisoners, enforced disappearances, restrictions on humanitarian assistance and recruitment of children. Since the attempts to put the Sri Lankan issue on the agenda of the United Nations Security Council failed on the unwillingness of China and Russia, the UN bodies in Geneva have taken a leading role in promoting the peacebuilding process on the island. The thesis aims to analyse the impact of the Geneva-based human rights bodies, in particular of the United Nations Human Rights Council, on the process of reconciliation in Sri Lanka, for which is crucial that the alleged violations of human rights in the last months of the civil war would be properly investigated and that the perpetrators would be held accountable. In order to put the activity of the UN Human Rights Council into broader context, the paper will examine the political development in Sri Lanka since the end of the civil war in 2009 as well as the failed efforts of Sri Lankan governments to launch the process of reconciliation.
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Policy development and implementation: case study of social housing
Hradecká, Tereza ; Vymětal, Petr (advisor) ; Kučera, František (referee)
The diploma thesis focuses on the process of creation and implementation of public policy which is examined on the social housing policy. The aim of this thesis is to explore how is a certain policy prepared and put into use, considering the social housing policy as an example. Therefore, the key research question inquires how the process of creation and implementation of public policy looks like. The first chapter of the thesis deals with theoretical background of the public policy process, stressing the role of public policy documents. The second chapter describes the area of Czech social politics and policy as a cover for social housing policy development. The third chapter examines the history of Czech housing policies. The final chapter focuses on the development of social housing policy and examines its connections with certain public policy documents.
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Výběr a implementace open source nástroje pro řízení portfolia projektů
Marek, Jan ; Chlapek, Dušan (advisor) ; Kučera, Jan (referee)
Methods and ways of implementation of changes and innovations in companies through project management are in today's society very well established. There exists methodologies, techniques and tools for the management of individual projects. However in a role of project manager, I very often faced the fact that companies are performing the project portfolio management in very intuitive way. This in itself leads to failed and prematurely terminated projects, initiation of the wrong projects or realisation of correct projects, but at the wrong time. Very often I have also recognized, that there is lack of awareness of fact, that there are also Open Source applications that can help with the organization's portfolio. This thesis deals with the definition of requirements, search and selection of proper OSS application and subsequent implementation. In the first part of this thesis I prepare a theoretical framework about PPM, and on that basis then identify and verify a set of requirements for selection of right OSS PPM application. The next section of thesis describes searching for suitable applications from a variety of sources, assessment against the requirements and the final selection. In the last part the implementation project is drafted, which aims to serve other colleagues in project management as one of the possible implementation paths. The outputs are then continuously confronted with experts in matter of projects and project management in the field of IT in order to maximize symbiosis between theory and real life experiences. Virtually every project management methodology defines the collection of already-proven techniques, best practices or lessons learned from the previous similar implementations. The main contribution of this thesis I see in fact that it contains not only the design of the project implementation, but also describes a logical path, what leads to the result. Therefore this thesis could be used as a base or discussed best practice, when a project of implementation of PPM application in place.
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The nursing process in a patient with cystic fibrosis
Václavková, Aleksandra ; Tomová, Šárka (advisor) ; Kaplická, Jitka (referee)
This bachelor ́s thesis deals with patients suffering from cystic fibrosis (CF). The thesis includes a theoretical and a practical part. In the theoretical one, underlying anatomical and physiological disturbances in key organ systems are discussed. A historical review, genetics aspects, manifestations of the disease and current treating possibilities are also concerned. Despite a considerable improvement on the field of the diagnostic process, the diagnosis of CF is still often considered to late with negative consequences on the prognosis. Although the causal therapy is still not existing, an intensive supportive care can significantly prolong and improve the quality of life of the patients. Considerable progress in the treatment research promises a total cure in the future. The practical part of the thesis contains an analysis of separates steps of the nursing process and its practical application in one concrete patient. I elaborated a nursing anamnesis according to the Gordon's functional health model. I elicited detailed information about patient's health state for establishing his complex evaluation. When the nursing problems determined and the diagnoses established, I worked on the realisation of defined nursing goals. According to the efficacy feedback, I continuously adapted the approach to the...
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Food supplements and iodine supplementation in pregnancy
VAVROUŠKOVÁ, Edita
Iodine is a trace element tremendously important for thyroid gland hormone production. It affects normal evolution of brain, especially in the time of intrauterine development and in the first year of child's life. Deficiency of iodine can lead to creation of pregnancy goiter, it also decrease the probability of carrying fetus to term and increase the probability of disorder in psycho-motoric evolution of child. Czech Republic is geographically lined up among the regions with lack of iodine in natural sources and food. However, nowadays the iodine deficiency is considered to be managed. But risk groups of population still exists, especially pregnant women can be endangered by lack of iodine, because the need of iodine intake in pregnancy increases to 200 250 ug per day and this level must be ensured. Consumption of food rich in iodine can cover the increased need of iodine. Significant source of iodine is milk and milk products. Another source is table salt enriched with iodine, but its daily intake is limited. Rich sources of iodine like sea fish, sea products and seaweed rarely occur in our diet. The goal of thesis was to evaluate intake of dietary supplements with iodine by pregnant women and evaluate women's awareness of higher iodine need and iodine food sources. Quantitative research in form of questionnaires was chosen for getting information from higher number of individuals. The questionnaire consists of 15 questions. Answers were gained from 118 respondents living in Central Bohemia, Moravia-Silesia region, Liberec region, region Highlands and South Bohemia. Questionnaire was distributed to gynecological ambulances by employees of State health institution. I visited gynecological ambulances in South Bohemia personally. By evaluation of questionnaire was found out that dietary supplement with iodine use 63 % pregnant women in researched population. All respondents always use only one type of dietary supplement. Among the most used belong "Chytré miminko", "GS mamavit" and "Femibion 800". 68 % of respondents know the importance of higher iodine intake in time of pregnancy. Question about daily recommended dosage was correctly answered only by 35 % of respondents. As a food sources of iodine the pregnant women know sea fishes, sea products, seaweed and table salt enriched with iodine. But 90 % of respondents are not familiar with milk and milk products as important source of iodine. Pregnant women should be better informed about importance of sufficient supply of iodine and about sources of iodine by gynecological ambulances. Information about mentioned issues should occur in magazines, education materials and internet articles more often. Respondents were notified of issues and important role of iodine in time of pregnancy by questionnaire. It is very probable that women found correct answers on the internet or in publication for expectant mothers after filling in the questionnaire.
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Comparison of cytokine production by leukocytes from newborns of healthy and allergic mothers
Dusilová, Adéla ; Hrdý, Jiří (advisor) ; Janatková, Ivana (referee)
The increasing incidence of children suffering from allergic diseases could be caused by sensitization of immature immune system during the intrauterine development. Several important scientific papers have demonstrated the ability of cord blood cells to respond by elevated proliferation activity after stimulation by common allergens. Following these findings, present study follows the production of cytokines which play a role in the pro- and anti-allergenic tuning of the immune system. Umbilical cord blood cells were stimulated with polyclonal activators (phytohaemagglutinin) and common allergens (ovalbumin, timothy grass, birch, mite). Subsequently, cytokine production was monitored using selected methods that reflect different stages of cell activation - at the level of mRNA by quantitative real time PCR (qRT-PCR), by flow cytometry detection of the presence of intracellular cytokines in different cell subpopulations and by ELISA measurement of cytokines in CBMC culture supernatants. The results obtained point to a very weak ability of these common allergens (timothy grass, birch, mite, ovalbumin) to stimulate CBMC to produce cytokines observed by all of these methodological procedures. Although we did not observe significant differences in CBMC cytokine production (IL-2, IL-4, IL-10, IL-12,...
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Cultural support in Prague's districts
Mokrá, Jolana ; KAŠPAR, David (advisor) ; PROKOP, Petr (referee)
Diploma thesis ”Cultural support in Prague's districts” focuses on a current situation in municipal strategical management and support of culture in all Prague districts. The introduction emphasises the importance of each level of government in the management and support of culture through comparing specific cultural policies. The research is divided into two parts. First one depicts how culture is being understood on the municipal level and presents an overview of existing instruments for support and planning. The second part is focused on a few municipal districts that handle their culture policy with a particular strategy and consider it to be an irreplaceable part of a local development. The second part also examines what tactics and tools these municipal districts use for a realization of their strategic goals. In conclusion, this thesis proves why the role of the local official government is so important in the developement and implementation of culture.
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