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Isolation and characterisation of light harvesting complexes from bacteria of the genus Gemmatimonas
DACHEV, Marko
Photosynthetic bacteria are highly abundant. The isolation and characterization of the light-harvesting complex from the Gemmatimonas phototrophica AP64T which belongs to the seventh phylum of the evolutionary important (bacterio)chlorophyll-containing bacteria are reported here. The purification of the complex was achieved by gel-filtration and electrophoresis. HPLC was used for identification of the pigments. The protein subunits were further identified by MS analysis.
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On-chip and capillary electrophoresis for cancer research
Hájková, Tereza ; Babula, Petr (referee) ; Provazník, Ivo (advisor)
The thesis deals with cancer disease and its genesis. The prostate cancer is the second most dangerous disease in male population. It is very important to detect it in early stage. This thesis deals with the proteins which are or could be used as potential cancer markers. For example in current commonly used the prostate-specific antigen and newly the metallothionein. Metallothionein is a protein occuring in fauna and flora in four basic isoforms. There is a hypothesis that the isoform content or/and their ratio may have a diagnostic implication and could be used in clinical practise. For this reason they are studied and developed methods for specific and sensitive analysis of individual isoforms. In this work, capillary electrophoresis is used for the separation of the isoforms. The influence of the parameters such as type of background electrolyte, its pH and separation voltage etc. on the final separation are discussed.
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Využití plemene bílá krátkosrstá koza v ČR
Minaříková, Marcela
In my bachelor thesis, entitled "Usage of breed white short-haired goat in Czech Republic", which is figure on origin of this breed and it's breed characteristic. In next part I will write about milk production of white short-haired goat and about factors, which influence this production. The main influences, which affect milk production, include breed, physical size, weight of the individual, age, the size and shape of the udder, lactation, date of birth, frequency of litter, nutrition and environmental temperature. In my thesis I also mention about population of goats in Czech republic and in this charter I describe which farms deal with breeding of short-haired goats and manufacturing milk products. Further in my thesis I look into chemical compounds of milk and description of its individual parts like proteins, carbohydrates, fats, minerals, vitamins and dry matter. Next part of my thesis is concentrated on methods of rating sof lactation and quality of milk which part is also a description of laboratory analysis for designation in compounds like dry matter, fats, lactose, minerals, proteins and somatic cells in milk.
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Selected fabric metabolism parameters of dairy cows
KAŇKA, Vladimír
The aim of this study was to evaluate antiketogenic prophylaxis of the veterinary medicine Kexxtone, in breeding of Holstein cows, in the company Agrodam Hořepník, s.r.o. There were then evaluated the parameters of a metabolic state in the herd monitored. Furthermore I evaluated the milk yield, amount of fats, proteins, lactose and somatic cells in the milk during 100 days of lactation and subsequently, the level of pregnancy after the 1st and the 2nd insemination was evaluated. The observation took place from July 2014 to December 2014 in a way that every two months was selected 8 cows and 8 heifers with a similar calving date. After that they were split into two groups i.e. into the experimental group (the kexxtone medicine was used) and into the control one. These cows were bled and milked. Subsequently, those biological samples were sent to a laboratory to be diagnosed. The higher milk yield was found within the experimental group and so as the content of milk constituents, i.e. fats and proteins. A beneficial effect of this antiketogenic medicine on the amount of ketones in blood and milk of animals was also confirmed within the experimental group. There were not found any significant statistical differences in other monitored parameters.
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INDICATORS OF MILK PRODUCTION AND FERTILITY BY GRAZING REARED HEIFERS
PALČISKOVÁ, Barbara
The aim of this thesis was to evaluate the parameters of the milk performance, reproduction indicators and health of Holstein cows that were raised on pasture at ŠZP (School agriculture company) Haklovy Dvory. Monitoring is carried out in the years 2011 - 2013. The work involved two groups bred cows grazed in total 56. In 2012 it was 27 cows and in 2013 29 cows. The control group which was represented by 20 heifer which had been reared in stable in years 2010-2011 the number 20 Holstein cows. The evaluation of parameters was set on the first standardized lactation in the range of 240 to 305 days. Into observation of reproductive indicators was included the age of the first calving (days), insemination interval (days) and servis period (days). From the productive indicators there was the quantity of milk (kg), the content of fat in the milk (%), quantity of fat in the milk (kg), and the content of proteins.
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Vliv plemene na množství a složení mléka dojnic chovaných v identických podmínkách na vybrané farmě
Müller, Luboš
The aim of this study was to determine the effect of breed on the amount and composition of milk of dairy cows kept in identical conditions on the selected farm. There were monitored cows of the Fleckvieh breed, the Holstein breed and the Ayrshire breed. The ration was given to all three breeds of dairy cows in the same quantity and quality. The results of milk yield were determined from the results of performance tests. There was observed the total milk yield in kilograms per 305 days, the average fat content and average protein content. The collected data were compared within breeds between them. Furthermore, the results were compared with the results of performance tests across the country. Most are yielding dairy cows of the Holstein breed, then they were spotted Fleckvieh least taken from their goats and cows ayrshirské. The highest content of components were Fleckvieh cows, Holstein cows followed by a minimum of fat and protein should Ayrshire cows. Compared with the control performance over the Czech Republic the best performing Fleckvieh cows, then Ayrshire and less than the national average taken from their goats Holstein cows.
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Vliv konzumace vybraných potravin sacharidové povahy na hladinu krevní glukózy
Langhammerová, Lucie
This thesis deals with the measurement of blood glucose levels after consumption of selected foods (glucose solution, milk chocolate, rice sandwiches with corn, fruit snacks with apricots and broken buckwheat multigrain bread) at regular intervals. The first part of this thesis is pursued to the literature review, which provides a comprehensive overview of the metabolism of carbohydrates, fats and proteins. It also discusses the differences in the carbohydrate metabolism of healthy people with diabetes type 1 and 2 It acquaints the reader with the concepts of blood glucose, glycemic index and glucose-tolerance test. The practical part includes the measurement of glucose in capillary blood and questionnaire survey concerning state of health, eating habits and the disease incidence in a selected group of volunteers. The experiment involved 12 students of Mendel University in Brno. Their blood glucose levels were measured at regular intervals (0, 30, 60, 90 and 120 minutes) after ingestion of the food. The glucose was chosen as a standard. From the measured values were calculated glycemic indices, which represented 100 for glucose, milk chocolate 40+-17,7, sandwiches, rice with corn 76+-25,8, fruit snacks with apricots 49+-17,9 and multigrain bread buckwheat 68+-21,3. The results were statistically processed and the effect of individual foods tested on blood glucose was evaluated.
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Validace nutričních parametrů uvedených na obalech vybraných potravin
Pláteníková, Markéta
The main purpose of this diploma thesis was to study legislative requirements about marking food products with nutrition labels and summarize the activities and procedures of supervising organizations connected to problematic of goods marking. One of the goals was to briefly describe technological and producing methods of selected food - ham, cheese, kephir milk, baby food with fruit, wafer, bread, wholemeal puffed bread and chocolate. Next goal was to evaluate the risks of food consumption with deceptive nutrition labels. In the practical part of this thesis I have determined quantity of saccharides, lipids, proteins and salt in selected food products by standard methods. Simple saccharides were determined by Schoorl's method and starch volume by Ewers's method. Lipids were determined by Soxhlet's extraction and after put through derivatization. In gas chromatograph (GC) were detected fatty acids. Apart from the Soxhlet's extraction was made also extraction according to Hara and Radin (1987) for comparison. Proteins were determined by method using nitrogen according to Kjehdal with adjustments according to Winkler. The amount of salt was detected analytically by argetometric precipitating titration. Determination the volume of nutritive parameters was made in three ranks of selected products. From obtained results were evaluated possible risks, which might be caused by consumption of food products with deceptively stated nutritive labels.
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