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Možnosti volnočasových aktivit žáků střední školy
Kalvodová, Petra
The bachelor thesis deals with the leisure activities of secondary school pupils. Final thesis is divided into two parts, theoretical and practical. The objective of the theoretical part consists in defining the key theoretical concepts that relate to given topic. Written document forms part of the general characteristics of free time, features and aspects of leisure time, including barriers experiencing free time secondary school students, complemented by a brief development of leisure education. The theoretical part includes the use of appropriate methods as the study of professional literature sources, production of extracts and their analysis, synthesis and comparison. The objective of the practical part is the revelation of the current ratio of minors to the use of leisure time, implemented through a questionnaire survey of students at the third and fourth year at selected secondary schools. The observed data are evaluated by simple statistical methods, shown by graphs and tables. The results are used to capture the benefits of leisure activities conducted for students, approach range of activities, an explanation of respondent participation in specific activities.
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Communication in Organizations
Tomíčková, Marta ; Šnýdrová, Ivana (advisor) ; Kopecký, Martin (referee)
6 Abstract This thesis aims to offer a comprehensive view of corporate communication while emphasizing its importance for everyday life of corporations and achievement of their strategic targets. The thesis introduces potential interpretations of corporate communication with the main focus on key moments of corporate communication as well as on consequences non-effective communication has on employee satisfaction and their life in the corporation. In the thesis I opted for the method of compilation and comparison of professional literature along with expert interviews which reveal the quality of internal communication in two selected corporations and prove one-sided view of communication inside corporations. The first part of the thesis provides a detailed look at characteristics of individual communication forms, knowledge of these characteristics being the cornerstone of further research in corporate communication. Besides, this part also describes the principles of active listening, a skill which is frequently omitted from the communication skill list. The second part deals with potential interpretations and divisions of corporate communication in professional literature and offers a thorough account of internal communication and its individual aspects. The following part looks at communication between...
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Interaction between TiAl melt and coated oxide refractories
Dočekalová, Kateřina ; Dlouhý, Antonín ; Zemčík, L. ; Fiala, J.
The present study focuses on reactions between ceramic crucibles and molten TiAl intermetallics. Coating strategies are investigated that exhibit a potential in slowing down the decomposition of the crucible wall. Uncoated crucibles (system TiAl melt - Al2O3 crucible) and crucibles with the coat (system TiAl melt - CaO coat - Al2O3 crucible) were used in melting and casting experiments. The reference state of the coated crucible before the melting trial was also investigated. Results of the SEM, EDAX and X-ray diffraction analysis contributed to the identification of the reaction zone and its chemical composition. The melt contamination with oxide inclusions results from the erosion process in which individual components of the melt diffuse along grain boundaries of the refractory and activate chemical reactions that decompose the crucible wall. Assessment of thermodynamic parameters associated with the chemical reactions helped in identifying the nature of the erosion process.
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Drugs and activities that could cause danger to life, health or damage to property
HANDSCHUHOVÁ, Silvie
The objective of this thesis is to summarize the effects of drugs, especially of the cannabinoids and amphetamine type stimulants, and to analyse which of these substances leads to high-risk activities, that could endanger life, health or result in damage to property. One of these activities, an important one, is driving under the influence of drugs. Driving itself is a complex demand, involving perception, decision making, psychomotor skills, knowledge, as well as attitudes and personality characteristics. Substance use and abuse has an impact on the functioning of the brain and mental processes and therefore on the performance of the driving task. Driving under the influence of drugs is a high risk activity which endangers not only the driver but also their surroundings. In the last few years, the Czech Republic registered a rapid increase in the number of impaired drivers, namely at police check points and in car accidents. The young drivers are mostly under influence of marijuana, respectively tetrahydrocannabinol (THC). Its negative effect on driving results from impaired co-ordination, slowed complex reaction time and in space and time distortion. The second group of drugs which can impair drivers is amphetamine type stimulants. Amphetamines are frequently sought by truck drivers, especially long-haul drivers, in Western Europe for its stimulative effects. In the Czech Republic the methamphetamine, Pervitin, is much more common. Intoxication is characterized by increased wakefulness, increased physical activity, increased respiration, rapid heart rate and an irregular heartbeat. The risk factors for driving are a lack of concentration and an overestimation of one{\crq}s own capabilities. After the acute phase of intoxication subsides, fatigue or states of narcolepsy may occur. Another amphetamine-based drug is methylenedioxymethamphetamine, MDMA or Ecstasy. This synthetic substance, chemically related to mescaline, is dangerous due to its ability to regulate body temperature. On rare but unpredictable occasions, this can lead to sharp hyperthermia, resulting in liver, kidney and cardiovascular system failure, and death. It can also cause hallucinations. All common drugs are easily detectable with ``drug test-cards{\crqq}, which are used during police checks around the the world. The Czech Republic is currently testing their utilization and is preparing legislation accordingly. Utilization of these tests during random checks could help to decrease the number of fatal road accidents and could help to decrease the number of impaired drivers on the roads.
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Preparation issues of teen-agers between the ages of 16-18 years
Smejkal, Jaroslav ; Buzek, Mario (advisor) ; Bošanský, Jiří (referee)
TITLE Preparation issues ofteen-agers between the ages of 16 - 18 years GOAL OF THE WORK The goals of my work were to outline the training model in the Czech Republic, monitoring of players complex effort of FC Victoria Pilsen, next the detection of negatives in game activities in matches and suggestion the training steps to removing realized negatives. METHODS Research was realized by direct and indirect observation methods, mainly using DVD records of teenagers 1st match. There were used qualitative and quantitative analyses of game activities. RESULTS Analysis should reveal some new facts. Results should define requirements for improvement oftechnical and tactical maturity ofindividua! in activities. KEYWORDS Game effort, qualitative and quantitative analysis, errors solution 3
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Characterization and stabilization of pancreatin
Wurstová, Agáta ; Němcová, Andrea (referee) ; Obruča, Stanislav (advisor)
This work focuses on a study of enzyme mixture pancreatin, its characterization and subsequent encapsulation into liposomes. As a reference proteins bovine serum albumin and trypsin were used. Characterization of pancreatin consisted of two parts. The first part focuses on optimization of methods for the concentration determination by absorption spectrophotometry using basic methods for identifying proteins (Biuret method, Hartree-Lowry method and Bradford method). Moreover, UV spectrums of the protein were measured. As a method for identification of protein´s molecular weight, SDS-PAGE was used. To identify components of pancreatin, LPLC was employed in two modifications, ion-exchange chromatography and size exclusion chromatography. The second part is dedicated to the characterization of pancreatin as enzyme in terms of pH and temperature optimum for the enzyme activities of protease (pH 9, 8 and 50 °C), amylase (pH 7 and 40 °C) and lipase (pH 7 and 50 °C). The last part of this work aimed at an encapsulation of pancreatin into liposomes and DLS analysis of distribution of particles and their zeta potential. Liposomes did not spontaneously release encapsulated enzyme. To confirm that proteins were successfully entrapped into liposomes, their structure was disrupted by application of phospholipase D. In conclusion, liposomes can be utilized as delivery systems for native enzymes.
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Sports centre Polička
Svoboda, Jan ; Kalánek, Jiří (referee) ; Brzoň, Roman (advisor)
This thesis deals with the design and elaboration process of a project documentation of a sports centre. The designed sports centre building is situated in the southern part of the town of Polička, in the area determined for sports and free time activities complexes and areas. It is a two-floor, non-cellar, flat-roof building. It is based on plain concrete strip foundations and reinforced concrete foundation footing. The bearing, peripheral and partition walls are designed from POROTHERM hollow clay blocks. Reinforced concrete columns are designated in open disposition areas. The ceiling construction is made from SPIROLL pre-stressed concrete floor slabs and point-supported reinforced concrete slabs. The peripheral walls of the first aboveground floor are insulated with external thermal insulation system with a protective gabion facade. The peripheral wall thermal insulation in a bowling restaurant and gym halls extension is designed from a ventilated facade with CEMBRIT METRO facade cladding panels. The building is functionally divided into two main parts with a shared entrance. The sports centre with gyms and other sports facilities form the first part. Three squash courts, a mini-football piste, a special room for spinning and alpinning, a room for group exercising and cloakrooms with sanitary facilities are designed within one part of the sports centre. A restaurant with a bowling area and a kitchen with storerooms and other facilities for employees form the second part. The designed complex is barrier-free. There is aimed to be a car park for fifty cars, four motorbikes and a bus in front of the building. Three parking places are for disabled people.
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Structure-Antifungal Activity Relationships in Substituted Butenolides
Šenel, Petr ; Pour, Milan (advisor) ; Hampl, František (referee) ; Doležal, Martin (referee)
Within the framework of this Thesis, several series of 3-(4-bromophenyl)-2,5- -dihydrofuran-2-ones with various substituents at C(5) derived from in vitro antifungally active 3-(4-bromophenyl)-5-acetyloxymethyl-2,5-dihydrofuran-2-one were prepared with the aim of further development of potential antifungals based on this lead. Primarily, we focused on the synthesis of furanones bearing alkylidene or alkoxymethyl/aryloxymethyl moiety in position 5. We found that 5-acyloxymethyl and 5-aryloxymethyl furanone derivatives undergo elimination leading to 3-(4-bromophenyl)-5-methylene-2,5-dihydrofuran-2-one under the antifungal screening conditions. This compound initiates fungal cell membrane disruption and is responsible for the antifungal activity of the former. The 5-alkoxymethyl analogues are unable to undergo the elimination process and are therefore inactive. The antifungal activity of 5- alkylidene furanones depends on the substitution of alkylidene side chain. Some of the target compounds displayed interesting cytostatic activities against HeLa S3 and CCRF-CEM cells (IC50 < 5 µmol.L-1 ). Furthermore, syntheses of 4-substituted or 4,5-disubstituted-3-(4-bromophenyl)-2,5- -dihydrofuran-2-ones were carried out. Some of the prepared derivatives possessed notable antifungal or antimicrobial activity.
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T cells labelling by bimodal contrast agent for in vivo studies of stroke
Krijt, Matyáš ; Poljaková, Jitka (advisor) ; Kříž, Jan (referee)
Stroke is a serious brain injury, which causes sudden death or terminates in permanent neurological disability. Nowadays, tissue plasminogen activator (tPA) is used as the only effective treatment of stroke. One of the potential targets for novel therapy are T cells. Even though the explicit role of T cells in the pathogenesis of brain injury, amounts and timing of all T cell subtypes infiltrating into brain during the stroke still needs further investigation. The research in this field is complicated by the lack of efficient methods for in vivo cell tracking. Therefore the aim of this thesis was to develop a method of T cells labelling by MRI contrast agent in order to investigate T cells distribution in ischemic mice model using in vivo MR imaging. T cells were isolated from C57/BL6 mice in two step isolation protocol using gradient centrifugation and magnetic separation with the efficiency of 97 %. The isolated cells were labelled with 100 μg Fe/mL of Molday ION Rhodamine B contrast agent. The labelling efficiency after 17 hours of cells incubation was higher than 99 %. The labelled cells were cultured with CD3 and CD28 antibodies resulting into the 74 % viability of labelled T cells compared to 83 % viability of non labelled T cells. The labelled T cells were visualized by fluorescent...
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Ceramides and Dimethylamino Acid Derivatives as Skin Barrier Modulators
Novotný, Jakub ; Vávrová, Kateřina (advisor) ; Sedlák, Miloš (referee) ; Doležal, Pavel (referee)
The purpose of this work is to contribute to the understanding of the structure-activity relationships and behavior of the skin ceramides and transdermal penetration enhancers based on dimethylamino acid esters. First two chapters provide a theoretical background for understanding the main principles of transdermal drug delivery as well as composition and function of stratum corneum barrier. The experimental work is presented in the form of individual publications in chapters three to five. The first chapter describes transport routes through the human skin and gives an overview of enhancers of transdermal permeation with main accent on their chemical structure, mechanism of action, interaction with stratum corneum components and advantages and drawbacks of their use. The second chapter brings more detailed information on the specific content and composition of stratum corneum. The main attention is paid to the ceramides - a complex group of lipids that play a crucial role as cell signaling molecules and skin barrier constituents. In the skin, these sphingolipids form a major part of the stratum corneum intercellular lipid matrix, which is the barrier for penetration of most compounds. The development of such a protective layer was a critical step in the evolution of life on a dry land. Moreover,...
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