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Range load in the casualty department
SEMECKÁ, Barbora
This bachelor´s thesis looks at the range load in the casualty department. The nursing care is focused on working with people, especially with the sick and is highly demanding, both physically and mentally. When working as a nurse, it is required to be professional and also have the ability of dealing with the unexpected and difficult situations. The knowledge of nursing care and the specific care in the injury area are also very important aspects. Nurses hold a position of responsibility and other people rely on them, they have to be responsible and accountable for their own decisions and actions as well as the results of their work have to be flawless. Another very important point is the mutual collaboration between nurses and local doctors, because just with the mutual help effective and quality care can be achieved.Two aims have been established in this thesis. The first aim: To map out the range of the nursing load in the casualty department. The second aim: To find out if nurses feel more physical or mental stress. Two research questions have been posed. The first research question: What is the nursing load in the casualty department? The second research question: Do nurses in the casualty department perceive more the physical or mental load? The practical part of this bachelor thesis involves the result of the qualitative research investigation. The results have been obtained through a semi-structured interview, which had been realized with the help of nurses who work in the casualty department in the South Bohemian region. In total twelve nurses have been spoken to. All the informations was completely anonymous and all of the respondents had the chance to reject being part of this interview. This interview, which has been made in advance, contained 21 questions in general. The basic identification date have been abducted at the beginning.
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The analysis of price elasticity of demand for beer
Hromadníková, Kateřina ; Mirvald, Michal (advisor) ; Babin, Jan (referee)
The thesis analyses price elasticity of beer demand. Hypothesis about inelastic demand is tested first for nationwide level and then on data of specific brewery. Elasticity was determined by regression analysis, specifically by ordinary least squares with all variables expressed in logarithmic form. Consumption of beer is the endogenous variable and price of bottled beer (price of one hectoliter of beer in case of brewery), average gross income and price level are in the role of the exogenous variables. The hypothesis about inelastic demand was successfully proved. Price elasticity estimates range from -0,66 to -0,2. In case of specific brewery price was not significant. On the other hand, average gross income seems to be the significant determinant. According to income elasticity beer seems to be luxury good in case of specific brewery and necessity in case of nationwide level.
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The course of microsporidiosis caused by Encephalitozoon cuniculi in experimental model
VOTOČKOVÁ, Tereza
Microsporidia are obligate intracellular parasites causing disease called microsporidiosis. The infectious strategy is a spore - a highly organized cell which gives a rise to the infection of the host. This bachelor thesis records the progress of microsporidiosis provoked by the infection of the experimental model of the species of microsporidia Encephalitozoon cuniculi, namely of the genotype ECIII. The theoretical part includes the familiarization with microsporidia. In this part, there are first records leading to paying more attention to them and initiating their closer examination. This section describes both the biological characteristics and the systematic categorization of microsporidia and the description of a spore as the infectious stage of life cycle, which enables get into the host body where microsporidia reproduce and cause diseases. The sources of microsporidia are mentioned as well as the ways of transmission and microsporidia species most commonly detected in human whose attention is devoted mainly to Encephalitozoon cuniculi - its history and development, the infectious progress in hosts, the detection, and the treatment. The practical part describes the methodical process of the way of monitoring of microsporidiosis on experimental models in a form of laboratory mice of inbred immunocompetent BALB/c and the mice with severe combined immunodeficiency SCID. The mice were orally infected with spores of Encephalitozoon cuniculi genotype ECIII, which was isolated from steppe lemming (Lagurus lagurus). The progress of infection was monitored both on the basis of coprological examination of mouse feces and on the basis of the examination of tissue and body fluid samples. The processing of individual samples was carried out with the aid of molecular diagnostics in the parasitological laboratory AV ČR in České Budějovice. The DNA from individual tissues and body fluids was isolated by using a commercially supplied kit, QIAamp DNeasy Blood & Tissue Kit ( QIAGEN), the feces were treated with a commercially supplied QIAamp DNA Stool Mini Kit (QIAGEN). The principle was to destroy the individual spores and to obtain the pure DNA which was suitable for further processing. Subsequently, the two-step polymerase chain reaction was carried out. providing rapid multiplication of DNA. The next step was a gel electrophoresis which arouses the division of the DNA into individual fragments on the basis of different molecular weights due to the effect of unidirectional electrical current. The fragments were visualized by UV transilluminator at a wavelength of 312 nm linked to a computer. The part of the research was the albendazole treatment of BALB/c mice. The drug was orally administered in the range from 28th to 42nd day after the infection. The results on progress of microsporidiosis through various organs and tissues, including the involved treatment, were recorded in well arranged tables. At the end of this bachelor thesis, there is a summary of the research and its comparison with previous studies. The results showed that microsporidia may be a threat not only for people suffering from immune deficiency, but thanks to their successful survival in the organs of immunocompetent hosts and their ability of activation from the undetectable level, they emphasize the danger of latent microsporidiosis as a risk and life-threatening factor for groups of people undergoing chemotherapy or organ transplantation where the patients can receive inflected graft from a donor.
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Analysing company's behaviour and communication on social networks
Němec, Milan ; Molnár, Zdeněk (advisor) ; Jelínek, Ivan (referee)
This thesis focuses on measuring company´s communication and behaviour on social networks. To capture the interaction between the company and its users the thesis uses web analytics tools. Therefore, the main objective of the thesis is analysis of these tools. To achieve this goal, the thesis characterizes social networks and their marketing opportunities for business promotion. Thanks to this, needs of using these tools is identified. The needs are: competitive analysis, optimizing communication, measuring achievement of objectives or detection competitive viral content. Research questions were determined and then analysis tools found answers to research questions. It was found that the tools provide various views of data and create a complete system for measuring and analysing behaviour and communication companies on social networks. The results were validated in a case study focused on the Facebook profile of the University of Economics in Prague. The study analysed the interaction profile of the school and its users and compared with others schools. In the results the analysis tools are validated and it is designed recommendation for improve engagement. This thesis revealed strengths and weaknesses of tools for measuring company´s communication and behaviour on social networks. It was found that these tools have wide range of use. They can be used for understanding the company's profile, its users, for purposes of competitive analysis, monitor corporate goals and optimize communications on social networks.
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Capillary electrophoresis with contactless conductivity detection for rapid determination of meldonium in urine
Šlampová, Andrea ; Kubáň, Pavel
A simple, rapid and cheap capillary electrophoresis-capacitively coupled contactless\nconductivity detection (CE-C4D) method for determination of meldonium (MEL) in\nurine samples was developed. Sample pretreatment was minimized to dilution of urine\nsamples with DI water and MEL was determined by CE-C4D in background electrolyte\nconsisting of 2 M acetic acid (pH 2.3) in ng mL−1 to mg mL−1 concentration range.\nSample-to-sample analysis time was less than 4 min, limit of detection and\nquantification was 0.015 and 0.05 μg mL−1, respectively, and the method showed\nexcellent linearity (r2 ≥ 0.9998), recovery (97.6–99.9%) and intraday as well as interday\nrepeatability (RSD ≤ 4.7%).
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Effect of low doses of herbicide paraquat on antioxidant defense in Drosophila
SÁBOVÁ, Michala
Disruption of cell equilibrium between production of free radicals and antioxidant defence is named oxidative stress. The main component of antioxidant mechanism is activity of antioxidant enzymes, which include superoxide dismutase (SOD) and catalase. In this study we evaluated the effect of herbicide paraquat on response of SOD and catalase, their transcription level and locomotion activity in Drosophila melanogaster. We exposed the flies to a wide range of paraquat concentration. Our results revealed that transcript and enzymatic levels of both SOD and catalase have a similar biphasic dose response with the peaks at 2,5 ?M paraquat concentration, resembling hormetic effect. Then, males were more sensitive than females. However, females had an increased locomotion activity. We found that paraquat susceptibility is increased in males and mating flies. Therefore, this study supports hypothesis of stress sensitivity elevation as a physiological cost of reproduction.
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Sorption and Stabilization of Metals/Mettalloids by Innovative Synthesized Sorbent Amochar.
Ouředníček, P. ; Trakal, L. ; Komárek, M. ; Pohořelý, Michael
Remediation of contaminated soil which is based on stabilization and immobilization of potential\nhazardous substance by sorption materials has been studied intensively nowadays. Biochar – activated organic carbon belongs to this group of stabilizing agents which can adsorb wide range of contaminants, including metals/metalloids. Surface area of the biochars is quite large in general and functional groups (e.g. COO–) can form chelates or alkaline elements on the surface, which is represented by cation exchange capacity. Altogether with the high pH values (7.00 – 10.00), biochars are quite effective sorbents and can adsorb metals/metalloids from the solution (ground water), especially in acidic soils (in the environments affected by intensive mining activities). Sorption\neffectiveness can be increased (especially for As (V) or Cr (VI) sorption) by modification of biochar by various types of secondary oxides. Innovative sorbent AMOchar (AMO + biochar) has been synthesized currently. The product was prepared by adding of biochar to the reaction solution during amorphous manganese oxide (AMO) synthesis. The AMOchar was formed mainly by Mn-oxalates which had coated surface of the pristine biochar. AMOchar composite was able to remove significantly higher amounts of various metal(loid)s from the solution despite the rather high pH of the material. Sorption effectiveness was high not only in case of Pb (II) sorption (almost 99%), and Cd(II) (51.2%), but also a very high amount of As(V), 91.4%. Additionally, both AMOchar composite was able to reduce Mn leaching. This can avoid potential post-contamination caused by the dissolution of less stable Mn-oxalates as observed in the pure AMO.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_22316111113040 - PDF
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PM2.5 Chemical Composition at the Rural Background Site Košetice.
Schwarz, Jaroslav ; Cusack, Michael ; Karban, Jindřich ; Chalupníčková, E. ; Havránek, Vladimír ; Smolík, Jiří ; Ždímal, Vladimír
Measurements of atmospheric aerosols at rural background areas are important because they are not influenced by the urban emissions, and are therefore better suited to investigate the influence of long-range transport and trends in concentrations of individual pollutants. Summary of the measurements of total concentration and the concentration of PM10 and PM2.5 components was carried out in the works Putaud et al. (2004, 2010). Although the later work included the measurement of more than 60 stations across Europe, the post-communist countries contained data on PM2.5 from only four locations (Prague, Debrecen, Hungary, and two rural locations). Although Košetice place where this study was made, is also discussed there, it was for only PM10 and total carbon (TC). Therefore, the aim of this study is to fill this information gap, and provide additional information regarding the PM2.5 fraction and composition at the Central European background station Košetice.
Fulltext: content.csg - PDF
Plný tet: SKMBT_C22016110313110 - PDF
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The occurrence of domestic and wild forms of the camels in the old world and their economic use
Burgerová, Michaela ; Gardiánová, Ivana (advisor) ; Jan, Jan (referee)
Genus camel (Camelus) includes two species, the Dromedary camel (Camelus dromedarius) and Bactrian camel (Camelus bactrianus). Camels have a wide range of utilization. People in Arabia countries but also in India and Egypt are using camels as a milk and meat animal, or even as a transport and companion animals and riding animals too. The aim of this thesis was to find sources about camel keeping and using and his role of farm animal in different cultures and countries. And about conditions and requirements in captivity. The first part of thesis contains information about phylogeny and origin of the camel family, taxonomy of camels, short information about the history of domestication and using of camels. There are also described the two species in terms of species biology etc. plus a brief mention of camel hybrids and camel breeds. The second part focuses on the use of camels. The preffered and most common use is in the production of milk, other dairy products from camel milk, fermentation of milk. Another use is for meat performance, meat composition, describes products. His utilization i also useful for other parts such as hair wool and mane, they come mainly from bactrian camel (Camelus bactrianus). The last is working utilization of camels, carrying baggage. Then hobby use. The thesis describes the conditions of keeping camels in captivity, keeping camels in Somalia, requirements for the supply of water and food, housing conditions in the Czech Republic, it is particularly widespread keeping camels in zoos. In Czech Republic they are small private camel breeders. For comparison they are conditions for keeping camels in captivity in Australia.
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