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Synura echinulata (Synurales) - silica scale plasticity in natural populations influenced by environmental factors; accompanying scaled chrysophytes
Kreidlová, Jana ; Němcová, Yvonne (advisor) ; Hodač, Ladislav (referee)
The silica scale shape variation in Synura echinulata has been investigated only in laboratory conditions, so far. The aim of this thesis was to study scale shape variation in natural populations and to determine which environmental parameters influence the scale shape. The scale shape variation was investigated using landmark-based methods of geometric morphometrics. Scale shape changes related to environmental factors were analyzed using the two-block method PLS (Partial Least-Squares analysis) and adonis (R). The scale shape was mostly influenced by locality, pH and altitude. Shape distinguished populations of S. echinulata probably exist in different localities. The more distant localities are, the less similar are scales of S. echinulata in their shape. This scale shape variation is probably genetically determined, even thought all so far sequenced populations belong to the same species. High morphological disparity which was probably caused by anthropogenic pollution, was recorded in the locality Brdy. During sampling of the natural populations of Synura echinulata in the Czech Republic, several localities representing a significant reservoirs of species diversity, were found. Therefore, the thesis was extended and biodiversity research in the Czech Republic was added. A few samples were...
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Analysis of Financing Research and Development in the Czech Republic
Vacek, Jan ; Hnilica, Jiří (advisor) ; Sieber, Patrik (referee)
The topic of this bachelor Thesis is an Analysis of Financing Research and Development in the Czech Republic. Research and Development (R&D) plays an important role in improving the competitive ability of the Czech Republic, for it constitutes a source for increasing productivity, economic growth and employment. The results of R&D lead to creation of new products and services, which in return increases the quality of life of the Czech citizens. This bachelor thesis focuses on analysis of financing R&D in the Czech Republic. The objective is to find out who finances R&D in the Czech Republic, where do these funds go to, and what they are used on. This thesis also focuses on R&D employees and R&D departments in which they work. The bachelor thesis is concluded by comparison between the R&D data for Czech Republic and other countries form the Europian Union.
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Influence of meadow management on the migration behavior of selected species of butterflies
Bubová, Terezie ; Langrová, Iva (advisor) ; Farkač, Jan (referee)
Migrations are the key process and one of the basic biological features, which allow survival of endangered species in fragmented landscape. In the last decades, agriculture intensification, industry and urbanization caused that number of natural butterfly habitats has significantly decreased. The localities originally suitable for butterflies are currnetly converted to farmland or building plots. This PhD thesis aimed to find a compromise solution of meadow management, which should allow a commercial use and concurrently do not jeopardize the continued existence of endangered or threatened species. The main idea of this study was in the intentional emigration causing in the middle of flight period, which should lead into resettlement of the population to another (potentially more suitable) locality. Possibility of aimed manipulation with butterfly emigrations would be considered as a major breakthrough in their protection.
The investigated species Phengaris nausithous (Bergsträsser, 1779) and Phengaris teleius (Bergsträsser, 1779) are both considered to be flagship species for protection of European butterflies at open meadows. This study is based on research, which was carried out in 2013-2015 at 17 patch in localities Dolní Labe, Czech Republic. In above mentioned years, one selected meadow was mowed in the middle of flight season and the emigrations were then monitored using mark-release-recapture method. Results of population and migration characteristics were calculated in MARK 8.1 software. Subsequently, the effect of mowing on emigration (comparison of emigrations from the meadow before and after mowing) was evaluated using Statistica 13.
The hypotheses of this study were based on the assumption, that the loss of resources after well-timed mowing should increase the activity of adult butterflies. During the monitoring, number of emigrations was increased only in flight season 2015. Unfortunately, the statistical evaluation did not show significant effect of mowing on migrations. On the other hand, the hypothesis dealing with colonization of nearest meadows was confirmed.
Effect of intentional emigration caused by management interventions was unfortunately not proved. However, we found out, that even though the population at one mowed meadow decreased clearly, the metapopulation size at the locality remained more or less stable.
At investigated (mowed) meadow, there was observed a sharp decline of P. nausithous abundance. On the other hand, P. teleius population was only slightly reduced. It is therefore conceivable, that P. teleius are not as susceptible to innapropriate term of mowing. The results obtained in our research conclusively displayed no possible positive effect of mowing in the middle of flight season and a necessity of suitable management maintenance at localities inhabited by Phengaris butterflies.
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Nutritional care of children at pediatric clinics and hospitals
METELCOVÁ, Jana
The main goal of this work called Nutritional Care in Children's Hospital and Hospital facilities is to verify whether the nutritional standards created for older child patients meet the needs and are checked and if their physiological needs are fulfilled in chosen hospitals. The next objective is to evaluate the satisfaction of the hospitalised patients with the meals given to them by hospitals. The theoretical part of this work deals with nutritional care, therapeutic care and nutritional needs of adolescents. It further deals with nutritional risks and marginally mentions technological processes regarding meal preparation. The method of qualitative research was used by using questionnaires filled in by chosen children patients who were then interviewed. Children aged 10 to 14 were supposed to be on diet number 13, half of them were boys and the other half were girls. The research was conducted in the children's ward in the hospital in Jindřichův Hradec in December 2015 and in the same type of ward in the hospital in České Budějovice in February 2016. The initial number of respondents was five patients in each ward, then using snow ball sampling method their number increased into ten in each ward. The weekly menu according to which the meals were distributed was obtained from the catering facilities of the hospitals. By using the program "Nutriservis Profi" the contents of nutrients in the meals were obtained. The results gathered from the program "Nutriservis Profi" were compared with recommended values for particular age group defined by The Czech Society for Nutrition, so meeting the physiological needs of the patients could be evaluated. From the results it was found that both hospitals have problems with supplying enough fibre content, phosphorus and calcium in meals for children's patients. There is also sodium, potassium, cholesterol and vitamin C surplus in the menus. Thanks to the internship in both hospitals in Jindřichův Hradec and České Budějovice meeting the physiological needs of the children could be evaluated. Considering the fact that the previous evaluation revealed some imperfections, therapists from both hospitals should concentrate more on checking nutritional values in meals, consequently it was recommended to focus more on the variety of the menus and their improvement. There were also inadequacies in attendance at the children's ward. There was a comparison of the results from both hospitals, based on questionnaires and interviews from the children's point of view. Based on the data, children hospitalized in Jindřichův Hradec were more satisfied with the meals. In the conclusion there is a summary of the research and suggested suitable improvements for hospitals. There is a model menu for diet number 13 in the attachment which could be used by hospitals.
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Protection of personality in the legal system of the Czech republic
Houdek, Miroslav ; Uhlík, Milan (advisor) ; Pavla, Pavla (referee)
The thesis deals with the protection of personality in the legal system of the Czech Republic. Its aim is to specify the possible ways protection of personality in the legal system of the Czech Republic with using of appropriate methods; and also to determine the number of reported and the Czech Police investigated cases including their treatment and in case of deficiencies in the legislation, organization and management are found, to propose measures to eliminate them. The theoretical part contains international and national legislation regulating protection of personality in the legal system of the Czech Republic. In the practical part there is an evaluation of legislation at first, followed by proposed measures to eliminate deficiencies in the field protection of personality. This is followed by analysis of wiretapping and recording of telecommunication operations and analysis of statistical data of selected cases undertaken by the CZ Police. At the end of the empirical part there is performed a quantitative research by using a questionnaire that is intended to members of police and citizens. The resulting values are analyzed, evaluated and translated into charts and graphs. In conclusion there is a proposed change in the law in the sense of de lege ferenda.
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Operations research methods in companies
Lisný, Jozef ; Kvasnička, Roman (advisor) ; Hlavatý, Robert (referee)
This thesis is focused on the application of operations research methods for selecting right accounting software for City Med Ltd., based on relevant criteria, which were determined by an expert of a company.
City Med Company Ltd. It is a private medical facility that provides outpatient and rehabilitation protection in some medical fields.
Bachelor thesis is divided into theoretical and practical part.
The theoretical part explains basic concepts, such as operational research, multi-criteria decision making s or multi-criteria variant analysis.
Methods of operational research are economic-mathematical methods that facilitate and accelerate work of individuals or businesses. Their continual development is proved by continuous invention of new methods of calculations.
Multi-criteria variant analysis is then used to select the best option from the final list of criteria.
In the practical part consist of calculations using operational research methods, scoring method and TOPSIS method, by criteria that were set by companys expert and final list of options of accounting programs.
In the end, it is decided which accounting software is the most convenient according to established criteria, and that one is recommended for implementation to City Med Ltd.
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The role of volunteering in non-governmental organizations working in the social sector
Papoušková, Daniela ; Varvažovská, Pavla (advisor) ; Pavel, Pavel (referee)
This diploma thesis was elaborated on the theme The role of volunteering in non-governmental organizations active in the social sphere.
As it is obvious from the title, the main theme was volunteering, volunteers and their active involvement in the non-governmental organizations in Hradec Kralove region. The aim of this work was to clarify and also realistically approach the real activity we generally call volunteering. The detailed view was focused on the target area of seniors.
The aim was to evaluate whether is this effort of volunteers in their final outcome for the social sector really benefit or whether it would be for nonprofit organizations expandable.
To fulfill the goal of this work have been chosen the method of quantitative and qualitative survey. For the quantitative research was chosen the form of the questionnaire, which was distributed through the web portal. For qualitative research was used the method of the semi structured interview. The qualitative survey was conducted by the research interview with the local nonprofit organizations.
The dissertation is divided into three parts. The first part deals with the concepts related to the specific topic. In the following part are processed the conducted surveys. The work is completed with the conclusion, which in conjunction with the obtained results of the investigation is commenting whole processed topic. These observed results help generally specify the situation of volunteering and nonprofit organizations in the Czech Republic.
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Electric Drive Diagnostics and Utilization in Agricultural Machinery
Čedík, Jakub ; Pexa, Martin (advisor) ; Kumhála, František (referee)
This doctoral dissertation thesis deals with rotary mowers, specifically with the issue of mulching.
In the theoretical part the reader is being familiar with the technology of mulching and its importance. Further, the individual types of mulchers and its advantages and disadvantages are described. The literary analysis of energy demands of rotary mowers is stated. Further, the parameters influencing energy demands of the mulcher are also analyzed.
The results of measurement of the energy demands of the mulcher with vertical axis of rotation are presented. The dependency of input power, specific energy consumption and fuel consumption on mass performance of the machine are created. Furthermore, the energy demands was determined, while mulcher was working continuously at the permanent grassland. The energy losses of the mulcher were also analyzed.
The effect of modification of operational and constructional parameters on the energy demands and quality of work of the mulcher wit vertical axis of rotation is studied. From the constructional parameters the shape of working tool, especially cloth angle and rake angle of the tool, and shape of the cover of the mulcher workspace is modified. From the operational parameters the cutting speed and mass performance according to conditions is modified.
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Mophological and molecular diversity of a tropical tree species Guazuma crinita in the Peruvian Amazon
Tuisima Coral, Lady Laura ; Lojka, Bohdan (advisor) ; Helena, Helena (referee)
Fast growing tree species Guazuma crinita (Malvaceae) was selected as a priority species for domestication in the Peruvian Amazon due its important contribution to the livelihood of local farmers. Its domestication process is still in an early age as for many tropical tree species little is known about its genetic variability and we dont know anything about the impact of domestication on its genetic resources. The main objective of this research was to assess the genetic variability of G. crinita within and among populations in the Peruvian Amazon by the use of morphological (wood physical traits) and molecular (ISSR and AFLP) markers.
Wood physical properties among six G. crinita provenances were evaluated. Wood samples were drilled from the base middle and top of the stem of 12 randomly selected eight-years-old trees for determination of wood measurement. Pearson correlations between physical properties were also determined. All wood physical properties except green density differed significantly among provenances. We also found statistically significant variation due to stem level position. The moderately dense wood and the coefficient of anisotropy (1.6) suggested that G. crinita has stable wood; they represent important advantages in terms of costs for transport and transformation process. The results suggested potential to select provenances with desirable wood properties for further breeding and domestication. Due to the variation found even in limited tree samples it is recommended further analysis with more extensive number of samples from different provenances and planting zones.
This research presents the first assessment of genetic variability based on inter-simple sequence repeat (ISSR) markers for 44 G. crinita genotypes from a clonal garden multiplication established in the Peruvian Amazon Research Institute (IIAP) in Ucayali region. Ten ISSR primers amplified a total of 65 bands of which 61 were polymorphic (93.8%). The range of DNA amplification varied from 260 to 2200 bp. Among the provenances overall genetic differentiation (Gst) was 0.03 indicating 97% of genetic diversity within provenances. Gene flow (Nm) was 12.9 alleles per generation. Cluster analysis was not related with geographic origin suggesting a common gene pool which was supported by calculation of weak positive correlation was found between genetic and geographic distance.
With the use of AFLP markers an insight on how domestication process does impact G. crinita genetic resources is also reported on this research work. I was able to generate fingerprint for 58 leaf samples representing eight provenances and three population types, 19 from a natural regenerated population 15 cultivated in home garden nursery and 24 from a collection of genotypes considered as semi-domesticated population. Seven selective AFLP primer combinations were used. A total of 171 fragments were amplified with 99.42% of polymorphism at species level. Each type of population generated fragments with 72.51% 49.12% and 54.39% of polymorphic fragments respectively. Neis genetic diversity and Shannon index information were found to be higher in the population of natural regeneration compared to overall semi-domesticated population (He = 0.10 and 0.9; I = 0.19 and 0.16 respectively). The analysis of molecular variation (AMOVA) showed higher variation within provenances rather than among (84% and 4% respectively). UPGMA clusters analysis and PCoA did not showed correspondence between genetic and geographic distance in addition their correlation was not significant. There was a significant genetic differentiation among types of population suggesting slight genetic bottleneck in semi-domesticated populations yet with relatively high levels of genetic variation.
In situ conservation for populations with high levels of genetic diversity was recommended. In addition proper management of natural regeneration and ex situ genotype collections might be a good conservation strategy to maintain G. crinita genetic resources.
The use of morphological (wood physical traits) and molecular markers were successful to reveal genetic variability of G. crinita and they could be used for other tropical tree species. For further researches it is emphasis to extent the number of samples and geographic scale.
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