National Repository of Grey Literature 12,409 records found  1 - 10nextend  jump to record: Search took 1.50 seconds. 

Observation of biological material in low voltage transmission electron microscope
Vancová, Marie ; Nebesářová, Jana
Different methods of specimen preparation for low voltage transmission electron microscopy (LVEM) were evaluated. The contrast of the image in LVEM, contrary to high voltage TEM, is strongly influenced by the thickness of the specimen. The resin embedding media used and the process of sectioning employed contributed to image quality and resolution.

Comparison of Business Intelligence implementation using open source solutions for middle size companies
Schmidt, Róbert ; Maryška, Miloš (advisor) ; Sládek, Pavel (referee)
The main goal of master thesis is to analyze and propose possible low cost Business Intelligence solution with open source technologies and comparison of available tools for implementation in middle size company. We compare Pentaho and Jaspersoft tools implemented on local hardware and cloud environment with Microsoft Azure services. The theoretical part focuses mainly on understanding the business intelligence and its architecture, because architecture is an important part of the work. Actual tools are designed as stand alone modules for specific activities in the business intelligence lifecycle. Low cost tools are often connected with open source technologies and cloud computing. This part of the work contains explanation of these terms and their advantages and disadvantages for our chosen target group of companies. The analytical part includes defined parameters by which it is conducted analysis of tools and their comparison. Business Intelligence solutions are divided according to arcitectural layers. The evaluation criteria are divided into financial, technical and user category. In conclusion, chosen tools are compared and evaluated. The main contribution of this thesis is comparison of open source business intelligence tools for implementation in middle size company. According to the EU directive, middle size company does not exceed 250 employees or profit is less than 50 million euros. The reader can compare the different solutions and their pitfalls or shortcomings that could be critical for the implementation.

Proposed marketing strategy of a low-cost airline
Khomutova, Sofia ; Král, Petr (advisor) ; Tročil, Jan (referee)
My Master thesis focuses on the design of the marketing strategy of a low-cost airline SparrowJet. The first chapter of the theoretical parts contains the required basics of theoretical information necessary to develop a marketing plan. In the second chapter is an analysis of the air transportation market with an emphasis on the low-cost segment. The final chapter of the theoretical part is dedicated to the marketing mix in the transportation. The practical part of my thesis begins with the situation analysis, which includes: the PEST analysis, Porters five forces analysis, a competition analysis and a customer analysis. My work is concluded with a complete proposal of a marketing strategy for the airline, including both basic strategy of S-T-P and a marketing mix.

ELECTRON BEAM REMELTING OF PLASMA SPRAYED ALUMINA COATINGS
Matějíček, Jiří ; Veverka, J. ; Čížek, J. ; Kouřil, J.
Plasma sprayed alumina coatings find numerous applications in various fields, where they enhance the properties of the base material. Examples include thermal barriers, wear resistance, electrical insulation, and diffusion and corrosion barriers. A typical structure of plasma sprayed coatings, containing a multitude of voids and imperfectly bonded interfaces, gives them unique properties - particularly low thermal conductivity, high strain tolerance, etc. However, for certain applications such as permeation barriers or wear resistance, these voids may be detrimental.\nThis paper reports on the first experiments with remelting of plasma sprayed alumina coatings by electron beam technology, with the purpose of densifying the coatings and thereby eliminating the voids. Throughout the study, several parameters of the e-beam device were varied - beam current, traverse velocity and number of passes. The treated coatings were observed by light and electron microscopy and the thickness, structure and surface morphology of the remelted layer were determined and correlated with the process parameters. Based on the first series of experiments, the e-beam settings leading to dense and smooth remelted layer of sufficient thickness were obtained. In this layer, a change of phase composition and a marked increase in hardness were observed.\n

Diagnostic options in the kindergarten
KAŠPÍRKOVÁ, Hana
In this thesis - Possibilities of diagnosis in nursery school ? I was deal with definition of pedagogical-psychological diagnosis and related terms. Here are too each developmental periods of preschool child, I described function of nursery school and I was focused on each diagnostical methods, which can use teachers in nursery school. In practical part I did observation of children in nursery school, I was focused on preschool children. I completed my observation by diagnostical tests and projective methods, which were evaluated in final phase. Next I performed the method of interview with each child. In the last part of practical section is overview of diagnosis methods, that are most used in nursery school.

Fluorescent analysis of the differential protein expression in normozoospermic and asthenozoospermic sperm samples
Děd, Lukáš ; Čapková, Jana ; Kubátová, Alena ; Teplá, O. ; Pěknicová, Jana
Asthenozoospermia is one of the main seminal pathologies underlying male infertility. Previous proteomic studies have demonstrated the significant differences in the protein profiles between normozoospermic and asthenozoospermic sperm samples. Since these studies were primarily focused on the identification of differentially expressed proteins by mass spectrometry, we aimed to evaluate the ability of our diagnostic antibodies to detect the differential expression of selected protein markers by fluorescent microscopy and flow cytometry techniques. Therefore, we analyzed sperm samples from 30 men with normal and 30 men with astheno spermiograms, average by the panel of our diagnostic anti-human sperm (Hs) antibodies. Fluorescent microscopy and flow cytometry analysis revealed quantitative differences in the protein abundances between normo and astheno sperm samples, namely, in GAPDHs, evaluated with Hs-8 MoAb, VCP, evaluated with Hs-14 MoAb, and ATP synthase, evaluated with MoAb Hs-36. From the methodological point of view, we observed very high correlation between the data obtained by fluorescent microscopy and flow cytometry techniques and therefore both methods are useful for evaluation of protein differences associated with asthenozoospermia. From the clinical point of view, we observed the strong association of the low sperm motility in the sample with the expression of proteins, playing an important role in sperm energy metabolism (expected), but also with the expression of all tested intra-acrosomal proteins. These findings further demonstrate asthenozoospermia as a complex semen disorder frequently associated with other semen pathologies, which are not diagnosed by basic semen analysis, and the possibility to use monoclonal antibodies as a tool for diagnosis of protein associated sperm pathologies in the semen with the low sperm motility.

: Time Requirement for Basic Nursing Care Procedures
SOUMAROVÁ, Iveta
A large number of nursing procedures are carried out in each ward in every hospital every day. In its theoretical part, this Bachelor thesis presents twenty basic procedures. The goal of the thesis was to find out what the most used nursing procedures are and to map the time consumption for individual nursing procedures. The quantitative research method was applied in the Bachelor thesis. Data were collected by measurement of time consumption of ten individual nursing procedures. These ten procedures were selected from a list of most used nursing procedures. Performance of the procedures was observed at the internal and surgical wards of the Hospital in České Budějovice. Both wards were observed over a 3-day span. Ten most often used nursing procedures were selected from the total number of procedures identical for both wards. Correctness of inclusion of the procedures was evaluated by the chi quadrat test in a contingency table. In addition, each of the ten most used procedures was measured ten times at each ward, from the preparation to the clean-up of instruments. The individual time values were averaged and presented along with conclusive deviations. A conformity with or difference in the time consumed was tested statistically by using the F or T test, where the applied significance level equals 5%. The research group was composed of the nurses and ancillary nursing personnel. Results are presented in graphs and tables. The following procedures differed in the individual wards: administration of medicines per os, serving of meals, venous blood sampling and blood pressure measurement. The bachelor thesis is focused on the time consumption for individual nursing procedures. The time consumption and the frequency of the individual procedures can serve as a base of explanation of the number of employees to the nursing management.

Changes to the contents of proline and relative electrolyte leakage of the selected genotypes of poppies depending on the influence by low temperature
Kudrna, Jiří ; Hnilička, František (advisor) ; Orsák, Matyáš (referee)
The object of this thesis is to study the effect of low temperatures, proline content and relative elektrolyte leakage in a selected range of varieties of opium poppy. Experiment included 18 poppy varieties with different contents of morphine. Poppy seeds for this experiment were provided by GB Oseva Pro s r.o., o.z.VÚO Opava a Český mák s.r.o. Poppy seeds were sown in containers of a size of 7,5x7,5 cm. Used cultivation medium is mixture of garden substrate A with quartz sand in a ratio of 2: 1. Plants were grown under controlled conditions in grow chamber Conviron E8. Before starting the experiment, the temperature was at 10 ° C during day and 5 ° C over night. Light cycle was 13 hours light and 11 hours darkness. The maximum value of irradiance was 800 micromol. The experiment was initiated in phase of rosette develoment. At this developmental stage were the plants exposed to frost temperatures (-5 ° C) over night and chill temperatures (10 ° C) duringthe day. After a week the plants were regenerated for 1 week at 5 ° C over night and 10 C during the day. All tested plants were in two day intervals measured of proline concentration after exposure to low temperature stress. At the same intervals as proline, were measured the values of relative elektrolyte leakage (Rel%).The obtained results show that the influence of low temperature causes increase of proline level. Control winter-crop variety Zeno exhibited the high kontent of proline (693.33 microg.g -1) in comparison with the spring poppy varieties. After regeneration were afected varieties Lazur, Opal, Buddha, Korneuburger Weisser, Aplaus, Major, Sokol, Zeno reduced the content of proline under the influence of higher temperatures. On the other hand the varieties Ofeus, Tatranský, Opex, Orbis, Maraton, Florián, Marianne and Albin were reversed with an increase of proline level. Proline concentration levels were constant and unchanged in case of varietes Postomi and Akvarel. Values of relative elektrolyte leakage were increased proportionately with length of exposure to low temperatures on the tested plants. The lowest value of membrane damage was after exposure to low temperatures observed in case of variety Zeno (23.53%). In the case of spring varieties was lowest membráně damage estimated in a variety Korneuburger Weisser (44.88%), while the highest was in the variety Orfeus (53.15%). It demonstrated the different responses of individual poppy varieties at low temperature. Most sensitive to low temperature were varieties Orfeus, Postomi, Akvarel, Orbis, Sokol and Marianne. As less sensitive to stress caused by low temperature seems to be spring varieties Opex, Buddha, Korneuburger Weisser, Albín.

Genetic Diversity of Semi-captive Population of Western Derby Eland (Taurotragus derbianus derbianus) in Senegal and Phylogenetical Relationships between Western Derby Eland (T. d. derbianus) and Eastern Giant Eland (T. d. gigas)
Zemanová, Hana ; Lukešová, Daniela (advisor) ; Jarmila, Jarmila (referee)
Representatives of family Bovidae are subjects of many studies concerning with their phylogeny, phylogeography, time of divergence or genetic diversity. Taxonomy is solved by comparison of morphological characteristics or by genetic approaches, genetic diversity could be solved by pedigree or by genetic analyses too. Tragelaphinae number nine species of two genera, Tragelaphus sp. and Taurotragus sp. The antelopes of the genus Taurotragus (T. derbianus and T. oryx) belong to the largest antelopes of the world. Derby eland (Taurotragus derbianus) has two subspecies, Western Derby eland (T. d. derbianus) and Eastern Giant eland (T. d. gigas), which are distinguished on the basis of morphological characteristics. Western subspecies (T. d. derbianus) is classified as critically endangered. There lives the only population in Niokolo Koba National Park in Senegal, which numbers fewer than 200 individuals. For the conservation, the semi-captive breeding programme has been established in 2000. It was created by six founders (one male and five females), which are presumed to be non-related. The population within this programme had 95 living individuals in 2013, living in seven herds in Bandia and Fathala reserves in Senegal. The population is under breeding management, which efforts to minimize kinship of the individuals. Studbook was established for the Western Derby eland (T. d. derbianus) in 2008 and is published annually. It acts about small population with low number of founders and no gene flow, which is threatened by inbreeding and genetic drift. Genetic diversity of the population was evaluated by means of microsatellite markers and the results were compared with the results of pedigree analysis. Pedigree analysis showed the highest genetic diversity in the generation of founders (FOUNDERS). It decreased in the generation of founders' offspring (OFFSPRING 1; born in season 2007/2008), due to the fact, that the only male took part in the reproduction. And it increased again in the generation of offspring of founders' offspring (OFFSPRING 2; born in the season 2009/2010), because more individuals were included into the reproduction. Fifteen individuals and five polymorphic microsatellite loci (from the total number of 13 tested loci) were chosen for the genetic study. The parameters of genetic diversity (HE and HO, Ar and deviations from Hardy-Weinberg equilibrium, and FIS and FST) were evaluated. Not any deviations from Hardy-Weinberg equilibrium were found out. The results of genetic analysis confirmed the highest genetic diversity in the population of founders (Ar = 2.79; HE = 0.664; HO = 0.750; FIS = --0.154). In both generations of offspring values of allelic richness and observed and expected heterozygosity decreased (Ar = 2.15; HO = 0.580; HE = 0.586 in OFFSPRING 1 and Ar = 2.14; HO = 0.370; HE = 0.480 in OFFSPRING 2). Contrary to the results of pedigree analysis, there was not been observed any improvement in OFFSPRING 2. The resultant values of genetic diversity parameters were quite satisfactory, despite of the low number of founders and mating of related individuals.

Testing of bioeffectors on phosphorus mobility in soils
Holečková, Zlata ; Balík, Jiří (advisor)
Crave for increase in agricultural production over the past sixty years reflects in widespread use of mineral fertilizers. Due to the growth of the world population an increase of overall demand for feed and food is expected. Limited availability of cultivatable soil and increasing dependance on mineral fertilizers can be also anticipated. It is therefore important to find alternative strategies for plant nutrition. In 2012, the project of cooperation between several universities in Europe was revealed. This project examines the use of bioeffectors in crop production. Use of these substances should help to reduce the input of mineral fertilizers used in agriculture and improve land usage. Thesis will perform testing in real conditions of different geographic locations. The main objective is to develop new approaches to the use of so-called bioeffectors based on the mechanism of action of living microorganisms and active natural substances. Bioeffectors may be, depending on soil and climatic conditions, a key factor for overcoming limitations in the availability of nutrients. They can contain microorganisms (bacteria, fungi) and active natural compounds (digest from soil or compost, microbial residues, plant extracts, metabolites from biological processes, dried herb material or seaweed). These substances are developed for a wide variety of crops (e.g. corn, wheat and tomatoes). Their effectiveness consists in nutrients mobilization from less accessible forms in the soil. The thesis is mainly focused on the impact of bioeffectors on various forms of phosphorus in the soil. Phosphorus in the soil belongs to essential elements necessary for the growth and development of plants. Despite its necessity in plant metabolism is its content in the soil relatively low. A higher phosphorus content is in most samples of soil mainly in the surface area because an increased biological activity occurs there causing the accumulation of organic material. Some influence can be also observed in connection with application of organic and mineral fertilizers. Amount of phosphorus in soil may vary depending on parental rock type, texture and land management (the ratio of P supplied and type and method of land cultivation). Above mentioned influences even have an effect on relative amount of organic and inorganic forms of phosphorus. Three basic groups of phosphorus contained in the soil can be described: inorganic phosphorus compounds, organically bound phosphorus and exchangeable absorbed phosphorus. The amount of phosphorus bound in the fractions depends mainly on the time of fertilizer application, including the impact of earlier interventions. For these reasons, it is necessary to execute detailed analysis of the various forms of phosphorus in the soil after application of mineral fertilizers together with bioeffectors. In this dissertation variety of pot and field experiments will be set up, where samples from each variation of soil and plant will be separated and researched. Samples will be analyzed and amounts of phosphorus will be measured.