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Spatial Diversity and Time-Frequency Orthogonality for Robust Wireless Communications
Zedka, Radim ; Armada, Ana Garcia (referee) ; Rakús,, Martin (referee) ; Götthans, Tomáš (advisor)
Předložená disertační práce přispívá do oblasti robustních bezdrátových komunikací dvěma zásadními nově navrženými technikami. První část práce je zaměřena na vývoj a analýzu nové techniky prostorové diverzity založené na nedávno objeveném konceptu časoprostorových kódů STLC. Na rozdíl od již známého ortogonálního STLC je tento návrh založen na kvazi-ortogonální kódové struktuře, která umožňuje použití čtyř antén přijímače namísto dvou, jak je tomu u ortogonálních STLC s plnou rychlostí. Práce obsahuje rigorózní teoretickou analýzu diverzitního zisku a asymptotického zisku poměru signál-šum (SNR) u nového STLC a porovnává je s dosud známými přístupy publikovanými v oblasti STLC. Druhá část se zabývá OTFS, v současnosti prudce se rozvíjející technikou přenosu dat s více nosnými, která vykazuje vynikající výsledky zejména v bezdrátové komunikaci uživatelů s vysokou mobilitou. Zde je prezentován nový systém založený na principu OTFS, který – na rozdíl od naprosté většiny OTFS publikací – jako jeden z klíčových návrhových parametrů využívá výkonovou spektrální hustotu (PSD) vysílaného signálu. Tento systém byl pojmenován UW-OTFS, protože jeho vnější stupeň je převzat z aktuálně rozšířeného systému UW-OFDM. UW-OTFS přebírá všechny výhody UW-OFDM, včetně vylepšené bitové chybovosti (BER) a definované spektrální výkonové hustoty zaručující výrazné omezení vyzařování do sousedních kanálů. S využitím konceptu OTFS navíc zaniká hlavní nevýhoda UW-OFDM, kterou je nemožnost použít ekvalizaci typu one-tap, tedy ekvalizace s jedním koeficientem. Systém UW-OTFS je navržen tak, aby pracoval v časově variantních kanálech s neceločíselným Dopplerovským posuvem, a to především díky využití modifikace známé metody BEM. Díky této metodě jsou jak odhad kanálu, tak ekvalizace prováděny technikou LMMSE. Navržený systém je následně analyzován jak teoreticky, tak numericky, a to pomocí metody Monte Carlo.
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Posouzení subchronické toxicity směsí pesticidů na raky
HOVORKOVÁ, Anna
Prometryn, belonging to the group of s-triazines, has been extensively used in the case of protection of maize production against broadleaf weeds and grasses. Its use was banned by the EU in 2004. Chloridazone is a pyridazone herbicide used to protect beet production and to control broadleaf weeds. Its properties increase the potential for the transfer of this pesticide to the aquatic environment. Together with its metabolites, it has been detected in groundwater in the Czech Republic. Its use in the Czech Republic has been banned since 2020. The adventitious properties and long-term use of these substances may increase the risk of contamination of non-target ecosystems by the pesticides used and their metabolites, which may form mixtures in this environment and thus have an even riskier impact on the environment. The aim of this work was to assess the subchronic toxicity of the pesticide mixture formed of the concentrations of these pesticides on the signal crayfish (Pacifastacus leniusculus) in comparison with the control and pesticides tested alone. A subchronic toxicity test of 20 days duration followed by 10 days depuration was used to obtain the results. The criteria evaluating the toxicity of the mixture were the biochemical profile of haemolymph, oxidative stress level and antioxidant enzyme activity from hepatopancreas, muscle and gill samples. The biochemical profile of haemolymph was significantly affected in individuals tested in the pesticide mixture. For biomarkers altered due to the mixture, suppression of the effect of prometryn in the mixture was observed in some cases. The analysis of antioxidant biomarkers did not show a higher toxicity of the mixture over the pesticides tested alone. In the case of antioxidants, a greater effect was observed for prometryn tested alone, where in some cases its presence in the mixture suppressed the effects of chloridazon in the mixture.
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Aktivita raka mramorovaného v závislosti na reprodukčním cyklu
MARKOVÁ, Kateřina
The aim of this thesis was to elaborate a literature review on the topic of crayfish activity, in particular their feeding activity and the use of shelters, including an emphasis on the role of sex and the reproductive cycle. I also focused on the characteristics of the marbled crayfish, Procambarus virginalis Lyko, 2017 and its usability as a model organism. The experimental part of the thesis focused on comparing three groups of female marbled crayfish (with ovulum, with glair glands and without ovulum). Experiments have been carried out using a method for ecological assessment of feeding behavior and predator-prey relationship in various prey densities in the form of larvae of buzzer midge Chironomus plumosus (Linnaeus, 1758) by functional response analysis and subsequently tested for the effect of shelter presence at uniform prey densities levels. In our experiment, all three groups of tested females showed a type II functional response typical of predators. The results indicate that females without ovulum have been the most active in terms of food collection in all cases, with a generally positive effect of the presence of shelter. Females with employed ovulum are generally less active in terms of consumption, processing and overall aktivity due to hormonal, physiological and consequently behavioural changes. Females with glair glands behaved similarly to females with ovulum in our experiments, being more cautious and less active than females without ovulum. This was probably due to their increased gonadosomatic index (GSI) and, conversely, their decreased hepatopankreas to total body weight ratio, which renders these females unable to physiologically ingest large amounts of submitted food. Female GSI decreases again after ovulation, nevertheless, even females with already employed ovulum did not consume more food, as females prefer safety at this stage of the reproductive cycle. They are less active, move more slowly, spend more time in a shelter, tend to their eggs, and thus cannot devote themselves fully to hunting and prey processing. Regarding its life strategy, the marbled crayfish is a generally suitable model organism for use in various areas of research, however, it is important to take into account its rapid life and reproductive cycle and adapt the conditions of the given experiments to this fact, as the parallel use of female marbled crayfish at various stages of the reproductive cycle can modulate results in terms of their activity. Certain differences in the presented results may also be due to the origin of the experimental animals, as with very few exceptions all performed experiments with marbled crayfish have been carried out using laboratory individuals. A number of conditions that change seasonally in nature have been kept constant in the laboratory. Therefore, it is important to keep in mind that in wild, crayfish are also exposed to both biotic (predation pressure of various species of fish, birds or mammals) and abiotic (changes in temperature and water chemistry) factors that can influence food activity. Nevertheless, we assume that the patterns observed here will also be valid for female crayfish living in the wild nature. However, the lack of similar experiments leading to the elucidation of female activity depending on the stage of the reproductive cycle provides a room to investigate other, not just crayfish species.
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Regenerace smyslových orgánů u raků
KOR, Golara
The antennae are a crucial sensory organ in crayfish that plays vital roles in their survival, communication, and orientation within their environment. They are equipped with sensory hairs that enable crayfish to detect various stimuli. Crayfish possess the unique ability to regenerate their antennae. Hemocytes are specialized cells within crayfish and play a crucial role in their immune system and contribute to the process of regeneration. We applied cryo-scanning and Transmission electron microscopies to study sensory organ morphology and regeneration in crayfish. In Chapter 2, cryo-scanning electron microscopy was used to identify potential differences in antennal morphology in six different crayfish species including marbled crayfish Procambarus virginalis, Mexican dwarf crayfish Cambarellus patzcuarensis, red swamp crayfish Procambarus clarkii, signal crayfish Pacifastacus leniusculus, common yabby Cherax destructor, and spiny-cheek crayfish Faxonius limosus. We observed significant differences among the six crayfish species in the ratios of antenna length, segment length and width to carapace length, and the number of segments. Examination of ultrastructural features unveiled differences in the distribution patterns of sensory hairs along the antenna and the morphology of the antennal surface. The varying morphology of antennae among studied species possibly indicates an adaptation to the specific conditions of their respective habitats. Furthermore, the outcomes demonstrated that a combination of variations in both antennal morphological characteristics and biometric measurements could effectively help us to distinguish the different studied crayfish species. In Chapter 3, the ultrastructural behaviour of hemocytes during coagulation and phagocytosis in the initial phases of injury in marbled crayfish was explored using transmission electron microscopy. During the coagulation process, hemocytes experienced marked transformations in morphology. The cytoplasmic granules exhibited a change from electron-dense to electron-lucent forms with the progress of coagulation. The transformed granules containing amorphous, electron-lucent material were observed to combine and release their contents into the extracellular space as part of the coagulation process. Additionally, it was observed that the nucleus contents also play a role in the coagulation process. Furthermore, the amputation of the leg led to substantial muscle degeneration, and phagocytic hemocytes start to take up the necrotic tissues. Besides, we observed the digested remains from phagocytized necrotic tissues merged into granules and other cellular components, thereby enhancing the granularity of the hemocytes and altering their cellular morphology. However, it is essential to note that hemocyte degranulation during coagulation could potentially reduce their granularity. Since morphological features are critical for classifying hemocytes, these morphological changes during coagulation and phagocytosis must be considered significant factors. The degenerated material that stored inside phagocytic hemocytes may have application in immunological and regeneration processes. In Chapter 4, we employed transmission electron microscopy to explore the ultrastructural aspects of potential immune cell involvement in nerve regeneration within crayfish antennae post-amputation. The findings indicated that, during nerve regeneration, all three forms of hemocytes were present. However, the granules within semi-granulocytes and granulocytes mainly contributed to the generation of new organelles such as mitochondria, the Golgi apparatus, and nerve fibers in the regenerated nerves of crayfish antennae. We revealed the transformation of granules of hemocytes into diverse organelles during the process of nerve regeneration at the ultrastructural level. In conclusion, these granules function as compact repositories of adaptable materials carried by immune cells, capable of transformatio
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Doom-Style Game
Rakus, Roman ; Vyskočil, Michal (referee) ; Pečiva, Jan (advisor)
Usage of effects in computer games is huge. Emphasis is put on the nicest pictures for the lowest price. The newest graphical adapters and technologies are used. In this thesis is described and implemented procedure how to create some effects such as particle systems, per-pixel lighting, normal bump mapping and fake volumetric lines with usage of OpenSceneGraph, shaders and others.
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High-Level Object Oriented Genetic Programming in Logistic Warehouse Optimization
Karásek, Jan ; Rakús,, Martin (referee) ; Cvrk, Lubomír (referee) ; Burget, Radim (advisor)
Disertační práce je zaměřena na optimalizaci průběhu pracovních operací v logistických skladech a distribučních centrech. Hlavním cílem je optimalizovat procesy plánování, rozvrhování a odbavování. Jelikož jde o problém patřící do třídy složitosti NP-težký, je výpočetně velmi náročné nalézt optimální řešení. Motivací pro řešení této práce je vyplnění pomyslné mezery mezi metodami zkoumanými na vědecké a akademické půdě a metodami používanými v produkčních komerčních prostředích. Jádro optimalizačního algoritmu je založeno na základě genetického programování řízeného bezkontextovou gramatikou. Hlavním přínosem této práce je a) navrhnout nový optimalizační algoritmus, který respektuje následující optimalizační podmínky: celkový čas zpracování, využití zdrojů, a zahlcení skladových uliček, které může nastat během zpracování úkolů, b) analyzovat historická data z provozu skladu a vyvinout sadu testovacích příkladů, které mohou sloužit jako referenční výsledky pro další výzkum, a dále c) pokusit se předčit stanovené referenční výsledky dosažené kvalifikovaným a trénovaným operačním manažerem jednoho z největších skladů ve střední Evropě.
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Localization and Rendering of Sound Sources in Acoustic Fields
Khaddour, Hasan ; Rakús, Martin (referee) ; Husník, Libor (referee) ; Schimmel, Jiří (advisor)
Disertační práce se zabývá lokalizací zdrojů zvuku a akustickým zoomem. Hlavním cílem této práce je navrhnout systém s akustickým zoomem, který přiblíží zvuk jednoho mluvčího mezi skupinou mluvčích, a to i když mluví současně. Tento systém je kompatibilní s technikou prostorového zvuku. Hlavní přínosy disertační práce jsou následující: 1. Návrh metody pro odhad více směrů přicházejícího zvuku. 2. Návrh metody pro akustické zoomování pomocí DirAC. 3. Návrh kombinovaného systému pomocí předchozích kroků, který může být použit v telekonferencích.
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Modern Methods of Time-Frequency Warping of Sound Signals
Trzos, Michal ; Rakús, Martin (referee) ; Průša, Zdeněk (referee) ; Schimmel, Jiří (advisor)
Tato práce se zabývá reprezentací nestacionárních harmonických signálů s časově proměnnými komponentami. Primárně je zaměřena na Harmonickou transformaci a jeji variantu se subkvadratickou výpočetní složitostí, Rychlou harmonickou transformaci. V této práci jsou prezentovány dva algoritmy využívající Rychlou harmonickou transformaci. Prvni používá jako metodu odhadu změny základního kmitočtu sbírané logaritmické spektrum a druhá používá metodu analýzy syntézou. Oba algoritmy jsou použity k analýze řečového segmentu pro porovnání vystupů. Nakonec je algoritmus využívající metody analýzy syntézou použit na reálné zvukové signály, aby bylo možné změřit zlepšení reprezentace kmitočtově modulovaných signálů za použití Harmonické transformace.
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Problematika invazních druhů raků na území České republiky
Čuprová, Veronika
The diploma thesis following the bachelor thesis "Invasive species in the Czech Republic" is focused on the issue of invasive species of crayfish. The aim of the thesis is to describe the legislation, the current method of monitoring these species and the possibilities of their regulation. The thesis deals with the monitoring of invasive crayfish species at two selected sites in the Czech Republic and the application of appropriate regulatory interventions. Along with the monitoring, water samples were taken and analyses for the presence of crayfish plague were carried out. The results show that at the Prostřední rybník site, where the marbled crayfish occurs and where eradication measures have been underway since 2020, it has still not been completely successfully eradicated. From the interview conducted with local respondents, it was found that about half of the respondents are aware of this issue, thanks to the actions carried out to eliminate this species and the information boards posted in the area. In addition to the signal crayfish, a small population of the native endangered river crayfish is also present at Besének. Regulatory measures have also been implemented at this site in order to eliminate this invasive species as much as possible and to prevent potential threats to the native crayfish. Based on the results obtained, further possible control measures were proposed at both sites. In the future, more focus should be placed on education of this highly topical issue, as public awareness and understanding is considered one of the most effective preventive measures.
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Buněčné a molekulární charakteristiky hemolymfy u raků
KIFAYATULLAH, NA
The cellular and molecular components of the hemolymph are the major arm of the innate immune system in decapod crustaceans. In-depth knowledge of the hemolymph components, including hemocytes and hemolymph proteins, can enhance our understanding of innate immunity in crustaceans. We utilized transmission electron microscopy and quantitative proteomics to study the cellular and molecular aspects of coagulation and phagocytosis in the hemolymph. Chapter 2 reviews the cellular and molecular parameters of the innate immune system and the effects of environmental stressors and their abiotic and biotic stress mechanisms in decapod crustaceans. The innate immune system of decapod crustaceans heavily relies on hemocytes in the circulating hemolymph. Generally, three types of hemocytes are accepted based on their morphology, however, there is still a lack of consensus among researchers regarding the classification of hemocyte types. The key innate immune functions such as coagulation and phagocytosis are still poorly understood and require further investigation especially at a molecular level. Environmental stressors can adversely affect the immune responses of decapod crustaceans, increasing their susceptibility to diseases. However, the abiotic stress mechanism is poorly understood due to the lack of available literature and needs further investigation. In Chapter 3, transmission electron microscopy was used to investigate the ultrastructural behavior of hemocytes during coagulation and phagocytosis in the early stages of leg amputation injury in marbled crayfish Procambarus virginalis. The granular hemocytes were the first to be activated, and the morphology of cytoplasmic granules changed from electron-dense to electron-lucent forms in an expanding manner. The transformed granules containing amorphous electron-lucent materials merged and discharged their contents into the extracellular space for coagulation. We observed that the leftover nucleus from degranulated hemocytes participates in the process of coagulation, which could be confused with hyalinocytes in some previous studies. In addition, leg amputation caused massive muscle degeneration, followed by a significant influx of phagocytic hemocytes that removed a substantial amount of muscle fibers and organelles, such as mitochondria, generated from disintegrating and decaying muscle. Furthermore, we found that phagocytic hemocytes contained varying numbers of granules in their cytoplasm and, for the first time, discovered that these cells incorporate necrotic bodies resulting from degenerated muscles into their organelles, such as cytoplasmic granules and nucleus. The granular hemocytes were found to be the main cells that carry out phagocytic activity in the injury site. This study provides a comprehensive description of all the stages of morphological changes in hemocytes during coagulation and phagocytosis after injury in crayfish for the first time. In Chapter 4, proteomic analysis of non-clotted and clotted samples indicated that quantities of most proteins remained unchanged during the coagulation process, suggesting that necessary proteins for coagulation are pre-synthesized and stored before clot formation. Due to their open circulatory system, decapod crustaceans possess robust clotting mechanisms. Upon injury, pre-synthesized clotting factors are released, resulting in clot formation. Therefore, only a few proteins, such as C-type lectin domain-containing proteins, Laminin A chain, and Tropomyosin, were down-regulated during clotting, suggesting their possible roles in the structural integrity of cells. Their downregulation could facilitate degranulation, a crucial step for clot formation.
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