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Cytotoxicity of selected naphthoquinones on prostatic cell cultures
Mondeková, Věra ; Gumulec, Jaromír (referee) ; Provazník, Ivo (advisor)
This master´s thesis discusses cytotoxicity of selected naphthoquinones on prostatic cell cultures. The introductory part is dedicated to general characteristic of naphthoquinones with focus on their cytotoxicity, testing of cytotoxicity and mechanisms of cytotoxicity. This part is followed by chapters about cytotoxicity, characteristics and biological activities of selected naphthoquinones; plumbagin and naphthazarin. The last part of this thesis’ theoretical section speaks about fluorescence microscopy and its use in research of naphthoquinones cytotoxicity. The practical part is dedicated to evaluation of cytotoxical tests’ results and to analysation of pictures of cells obtained by fluorescence microscope. At the end of thesis, all finding are summarized and put in the context.
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Image Segmentation Using Height Maps
Moučka, Milan ; Kršek, Přemysl (referee) ; Španěl, Michal (advisor)
This thesis deals with image segmentation of volumetric medical data. It describes a well-known watershed technique that has received much attention in the field of medical image processing. An application for a direct segmentation of 3D data is proposed and further implemented by using ITK and VTK toolkits. Several kinds of pre-processing steps used before the watershed method are presented and evaluated. The obtained results are further compared against manually annotated datasets by means of the F-Measure and discussed.
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Vegetation Detection in Images
Černá, Tereza ; Herout, Adam (referee) ; Přibyl, Bronislav (advisor)
This project focuses on detection of vegetation in digital image and describes approaches to detect vegetation. Created aplication uses grass detection method in video in real time. There is a new mean of evaluation of the method proposed in this project, using commonly used pixel by pixel detection and also a new detection approach, segment detection. Functionality of the application is checked by set of test images. The thesis is concluded by comparing results of those two approaches. Success of correctly detected vegetation ranges up to 86.32 %.
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Image segmentation based on relief
Gros, Jan ; Špiřík, Jan (referee) ; Křupka, Aleš (advisor)
The aim of this labor is to introduce the technique of image segmentation by flooding relief of landscape (watershed method). There are described some methods of image segmentation and explained the basic operations of the images, from which the morphological operations are used for watershed segmentation. Part of this labor is a short passage that deals with the use of some image filters for image preprocessing. The result of previous knowledge is presentation of segmentation algorithm designed by Lee Vincent and Pierre Soille. The next section describes the algorithm implementation in Java, contents and function of each class module and implementation using RapidMiner. The last section presents the results of the segmentation with created module using the test images without and with the use of some selected filters to reduce oversegmentation and improve the resulting segmentation.
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Image segmentation based on relief
Gros, Jan ; Špiřík, Jan (referee) ; Křupka, Aleš (advisor)
The aim of this labor is to introduce the technique of image segmentation by flooding relief of landscape (watershed method). There are described some methods of image segmentation and explained the basic operations of the images, from which the morphological operations are used for watershed segmentation. Part of this labor is a short passage that deals with the use of some image filters for image preprocessing. The result of previous knowledge is presentation of segmentation algorithm designed by Lee Vincent and Pierre Soille. The next section describes the algorithm implementation in Java, contents and function of each class module and implementation using RapidMiner. The last section presents the results of the segmentation with created module using the test images without and with the use of some selected filters to reduce oversegmentation and improve the resulting segmentation.
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|
|
Vegetation Detection in Images
Černá, Tereza ; Herout, Adam (referee) ; Přibyl, Bronislav (advisor)
This project focuses on detection of vegetation in digital image and describes approaches to detect vegetation. Created aplication uses grass detection method in video in real time. There is a new mean of evaluation of the method proposed in this project, using commonly used pixel by pixel detection and also a new detection approach, segment detection. Functionality of the application is checked by set of test images. The thesis is concluded by comparing results of those two approaches. Success of correctly detected vegetation ranges up to 86.32 %.
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|
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Image Segmentation Using Height Maps
Moučka, Milan ; Kršek, Přemysl (referee) ; Španěl, Michal (advisor)
This thesis deals with image segmentation of volumetric medical data. It describes a well-known watershed technique that has received much attention in the field of medical image processing. An application for a direct segmentation of 3D data is proposed and further implemented by using ITK and VTK toolkits. Several kinds of pre-processing steps used before the watershed method are presented and evaluated. The obtained results are further compared against manually annotated datasets by means of the F-Measure and discussed.
|
|
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Cytotoxicity of selected naphthoquinones on prostatic cell cultures
Mondeková, Věra ; Gumulec, Jaromír (referee) ; Provazník, Ivo (advisor)
This master´s thesis discusses cytotoxicity of selected naphthoquinones on prostatic cell cultures. The introductory part is dedicated to general characteristic of naphthoquinones with focus on their cytotoxicity, testing of cytotoxicity and mechanisms of cytotoxicity. This part is followed by chapters about cytotoxicity, characteristics and biological activities of selected naphthoquinones; plumbagin and naphthazarin. The last part of this thesis’ theoretical section speaks about fluorescence microscopy and its use in research of naphthoquinones cytotoxicity. The practical part is dedicated to evaluation of cytotoxical tests’ results and to analysation of pictures of cells obtained by fluorescence microscope. At the end of thesis, all finding are summarized and put in the context.
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