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Yeasts and wine
Palíková, Petra ; Vadkertiová, Renata (referee) ; Vránová, Dana (advisor)
This thesis deals with isolation and identification wine yeasts from grapes and must. For analysis was used white wine Sauvignon that was grown and producing after needs ecological agriculture. Remove samples were processed in laboratory and by the help of dilution method were obtained pure culture isolated yeasts. In the following step, by the application of commercial kit UltraCleanTM Microbial DNA Isolation Kit we were able to isolated individual DNA that it was used to the next analysis. Isolated DNA was amplification by PCR method with ITS1 and ITS4 primers. PCR products were detected on agarose gel. Amplification samples were chopped five restriction endonucleases: HaeIII, HinfI, TaqaI, AluI and MseI. Chopped DNA was detected by the same way as PCR products and it was compared with restriction patterns of collection yeasts. In the next step it was compared genetic similarity of isolated yeasts by using BioNumerics software. As a criterion it was used Pearson coefficients and UPGMA clastering analysis. The result is dedrogram of genetics similarity isolated yeasts.
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The viticulture Lahofer
Čech, Richard ; ing.arch. Lukáš Ležatka, Ph.D (referee) ; Pavlovský, Tomáš (advisor) ; Kacálek, Petr (advisor)
This bachelor thesis extends the bachelor’s studio project aimed at creating a survey on a new building for Vinařství Lanhofer company. The considered area lies in the territory of Dobšice near Znojmo and with its slightly rolling landscape is used for agricultural purposes. The mentioned trapezoid building site faces southwest, at the time was covered with vineyard and was bordered by minor roads connecting the plot to Dobšice and Suchohrdly. Building a new production site that would be able to produce up to 60 thousand bottles of wine, could be also used as storage space or would incorporate related services was intended. There were premises for public use, formal events or wine-tasting planned. The layout included restaurant with outside seating, sanitary facilities both for visitors and employees. Such a large-scale production of wine could be almost considered an industrial production that set up the main idea of the design. The basic clue of this project is searching an ideal shape of the production area that – in historical context of industrial buildings – is saw-shaped rooftop silhouette. The material concept of the building is based on visual and logical separating of production premises from public spaces that had been achieved by distinguishing both areas by using different facade cover. Stainless steel representing production environment and Cor-Ten patina steel panelling describing eternal wine maturation.
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Winery
Koláčková, Jana ; Havířová, Zdeňka (referee) ; Lavický, Miloš (advisor)
The diploma thesis deals with project of a new building of winery production. Winery has one floor and a basement. We can split the winery into three parts. First part – production. This whole part is dedicaded to the production of wine. It consists of spaces where grapes are degraded, fermented, pressed, stored, bottled, labeled and subsequently exported. Production has a volume of 300 000 l of wine. Second part is for administration. There are two offices and a confrence room. Third part is for public using and it is composed of the tasting room with view at the wineyard and of the retail hall. The structural system is prefabricated skeleton of reinforced concrete. The roof is constructed as a flat green roof.
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PROKES WINERY
Kunz, Ondřej ; Myslín,, Jiří (referee) ; Dýr, Petr (advisor) ; Čupr, Karel (advisor)
Based on the architectural study from the second year of the bachelor study program, the following bachelor thesis is elaborated, which further disseminates the study to other subsequent stages of the project documentation. The theme of the thesis is the new family viticulture in the village Velké Němčice for winery Kamil Prokeš. The building will include both production facilities and housing, accommodation for guests and a wine shop with an adjacent tasting room. The building is situated on the outskirts of Velké němčice, where there are old farm buildings and gardens, a football field and a one-storey garage parking lot. The land is unconstructed and currently there are only planted trees. On the sloping land is designed one object, which is divided into several parts. Wine production is located in the basement, which is accessible from the north side and allows the grain and expedition. The above-ground floor consists of three interconnected masses. The central part, where the wine cellar and tasting room are located, is designed as a single-story, glass-covered building with a flat, green roof. On both sides of the building there are two identical, two-storey buildings with a saddle roof, where the western building houses the wineries housing and the eastern accommodation for the guests. All three masses are visually interconnected by the overlapping flat roof of the central object. The unified building, which is completed on the northern and southern side with the terrace for the guests and owners of the winery, is created. In front of the building is a model vineyard, which refines the overall view of the facade of visible concrete and also unambiguously points out the function of the object. The bottom structure is designed as a monolithic reinforced concrete block and the above-ground floor consists of lightweight concrete panels, folded ceiling structures and a roof saddle roof. The central part has a core where the adjoining facilities are located,
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Honza`s Winery
Lavička, Jan ; Konečná, Petra (referee) ; Králová, Zuzana (advisor)
The diploma thesis deals with the design of a winery with accommodation facilities. The whole area consists of the main building, a building for storage of garden equipment and wooden cabins for lodging during summer time. The diploma thesis is mostly focused on the design of the main building with the winemaking facilities, five rooms on the second floor and technical background. All buildings in the area are appropriately placed considering the cardinal points and they are logically connected with each other by means of roads. The main building is fully provided with cellars where the largest part of the production and the sampling and presentation rooms are found. The shape of the floor plan displays a shape of the letter “u” with two above-ground floors. The whole second above-ground floor is created as a relaxation zone for the guests. Each room has its private balcony with a nice view of the neighbouring vineyards. The roofing is implemented by the flat roof with extensive vegetation and wooden roof trusses, fitting into the surrounding landscape.
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WINE HOUSE NIKOLSBURG MIKULOV
Kotoulková, Leona ; Macháčková, Šárka (referee) ; Dýr, Petr (advisor)
The topic of the diploma thesis is the design of a representative building of the Nikolsburg winery, which decided to get rid of the role of mediator of the grapes supply for another winery to produce its own wine. This building is to house a four-star hotel for thirty people, a tasting room with a view of the barrique barrels, a restaurant for forty people and a small wellness area. This newly designed building is located in the outskirts of Mikulov between the nature reserves Turold and Růžový vrch. The basic concept was created depending on the slope of the terrain and the orientation to the cardinal directions. I also tried my best to take advantage of attractive views and limit the view of the technical zone, which is located southwest of the plot. The façade made of ceramic fittings from the Flexbrick company helps me with this intention. An important expressive element of the building is the division of the last floor with hotel rooms, which are oriented to the surrounding landmarks - Mikulov with the Holy Hill and Růžový vrch.
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Winery and guest house
Mrkvicová, Kateřina ; Pospíšil, Tomáš (referee) ; Pěnčík, Jan (advisor)
In my thesis I have dealt with a building plan of a winery and a boarding house located in South Moravia. It is a detached house which is embedded in a mildly steep land. The building itself is barrier free. There are two floors and a basement. There is a production of wine in the basement. It is separated into several parts such as a clean preparation room, a storeroom for wine bottles and storeroom for Nitrogen bottles, hall, emploees´area, and export. The basement and the first floor are conected with an elavator. The first floor is supposed to serve the visitors. There is a reception, a large degustation room, bar, kitchen and the sanitary facilities on the first floor. On the second floor there is acommodation for visitors. There are seven rooms adapted for two or three people. The vertical brickwork in the basement is formed by concrete blocks 50 by producer BEST. The above - grade vertical brickwork is made of ceramic blocks POROTHERM PROFI 44. The bulding is insulated with thermal insulation system Baumit open Premier. The horizontal construction is made of ceiling panels SPIROLL. A roofing above the second floor is made of nailed ties. The roof has saddle shape and is double skinned. Total height of building is thirteen metres. In front of the building there is parking lot for twelve cars and one place for disabled.
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Moravian wine centre Brno
Jedrzejková, Ivana ; Myslín,, Jiří (referee) ; Dýr, Petr (advisor)
The main subject of this diploma project was to design an architectural proposal of Moravian Wine Centre in Brno, Czech Republic. To design a polyfunctional building with a concept of new use of an existing network of underground cellars became the main goal of the proposal. The building is divided into seven functional units where services in the fields of culture, education, gastronomy, recreation and wine production will be provided. The form and shape of MVC is based on the idea of making a passable connection between two important urban spaces Exhibition Centre Brno, and the Zluty Kopec area. The next aim was to create a public space for cultural events and recreation. Part of the assignment was to deal with smaller urban planning of adjacent properties and to react to Brno's planning policy. The new park type of green line, axially connecting those two points of Exhibition Centre and Zluty Kopec, and a new access road from the street "Vinarska" were proposed. Parking spaces are divided into above-ground and underground in order to restrict parking on street. An automatic parking system is part of the object MVC Brno.
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WINERY MARCINČÁK NOVOSEDLY
Zálešák, Tomáš ; Hudeček, Vladimír (referee) ; Dýr, Petr (advisor) ; Kacálek, Petr (advisor)
The theme of the bachelor's thesis was to design a new building project of the Marcinčák winery. The project was based on an architectural study which was made within the subject AG032 – Studio of Architectural Creation II and the main subject of the project was a new building construction in Novosedly, Moravia, region Břeclav. The land intended for this project is in the middle of the vineyard tracks in the area called Stará hora, near to the educational trail, where is currently the viewing area of Novosedly. The demands of Mr. Ing. Marcinčák for the construction included a space for processing the wine from the surrounding vineyards, its sale and a space for social purposes and accommodation. The construction is therefore a single unit divided into two floors. The main entrance is on the first floor, where is the access to the accommodation part with ten double rooms and one double room accessible for people with disability. In the other part of the building there is a social hall with sanitary facilities, catering kitchen and a tasting cellar. The entrance to the basement, where the wine factory is situated, was made to keep visitors in enjoyable and quiet resort and not bothered. The whole building is design in the shape U, which creates internal private space. At the same time the building shape protects the wine from the winds. In the middle of the building there is an observation accessible to the public, which maintains the view for the village and attracts more visitors or tourists on the natural trail.
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WINE HOUSE NIKOLSBURG MIKULOV
Matějík, Valeriia ; Vojtěšek, Jiří (referee) ; Dýr, Petr (advisor)
The goal of the diploma thesis is to design a wine hotel for 30 people with other public spaces with quality accommodation corresponding to the level of four stars in Mikulov. The new building of the Wine House is a three-storey building located in a sloping terrain, which consists of three floors above ground and one underground floor. The wine house will provide Nikolsburg Winery as a representative building. Visitors and guests of the hotel can enjoy a glass of wine on the terrace of the proposed building and at the same time wonderful views of the vineyards and the silhouette of Mikulov, relax in the surrounding picturesque landscape, or visit the nearby center of Mikulov, Pálava hills or take a boat ride on the surface of reservoirs called Novomlýnské nádrže. Accommodated guests will have the opportunity to use the wellness, massage and Finnish sauna. Tourists passing by will have the opportunity to taste good food and visit the wine lounge, where they can taste and buy locally produced wine at the same time.
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