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GUI for REPOMO
Šimek, Petr ; Růžička, Richard (referee) ; Sekanina, Lukáš (advisor)
Today, reconfigurable hardware is happend tendency when we can configure static circuit structure in the same way as software. REPOMO is a reconfigurable chip which contains 32 polymorphic gates. Polymorphism means that a gate can change its logic function as a response to the power supply voltage. The purpose of this thesis is to introduce the area of polymorphic circuits and construction and function of REPOMO chip. The main goal of the thesis is proposed and implemeted graphical user interface which make easy progress applications for chip REPOMO.
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Prediction of the Effect of Nucleotide Substitution Using Machine Learning
Šalanda, Ondřej ; Martínek, Tomáš (referee) ; Bendl, Jaroslav (advisor)
This thesis brings a new approach to the prediction of the effect of nucleotide polymorphism on human genome. The main goal is to create a new meta-classifier, which combines predictions of several already implemented software classifiers. The novelty of developed tool lies in using machine learning methods to find consensus over those tools, that would enhance accuracy and versatility of prediction. Final experiments show, that compared to the best integrated tool, the meta-classifier increases the area under ROC curve by 3,4 in average and normalized accuracy is improved by up to 7\,\%. The new classifying service is available at http://ll06.sci.muni.cz:6232/snpeffect/.
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The study of polymorphism and optimization of active pharmaceutical ingredients crystallisation
Novák, David ; Ing.Ladislav Cvak, Ph.D. (referee) ; Ing.Roman Gabriel (advisor)
Active pharmaceutical ingredients (APIs) are frequently delivered to the patient in the solid-state as part of an approved dosage form (tablets, capsules, etc.). Understanding and controlling the solid-state chemistry of APIs is therefore an important aspect of the drug development process. APIs can exist in a variety of distinct solid forms, including polymorphs, solvates, hydrates, co-crystals and amorphous solids. Each form displays unique physicochemical properties that can profoundly influence the bioavailability, manufacturability, stability and other performance characteristics of the drug. Most APIs are purified and isolated by crystallisation from an appropriate solvent during the final step in synthetic process. The main objective of a crystallisation process is to produce crystals with desired properties such as particle size distribution (PSD), shape and purity. All pharmaceutical dosage forms must be produced in uniform units, and good content of uniformity is only possible when the size of the active component is carefully controlled. For on-line control of crystallisations of Quetiapine Fumarate to achieve desired PSD and no changed physicochemical purity was used the Lasentec Focus Beam Reflectance Measurement (FBRM) system.
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Methodical aspects of genetic diversity of cattle
MIJAILOVIĆ, Angelina
The aim of this thesis is to describe methods for assessing genetic diversity. At the beginning is described biological diversity and its distribution with focus on genetic diversity. Furthermore, mutations, segregation, recombination, migration, selection, genetic drift, and inbreeding are described. The work also deals with the polymorphisms including SNP, VNTR and microsatellites. They are described as genetic markers, marker assisted selection and genomic selection. For within-population genetic diversity the text deals with biometric methods, the part describing interpopulation genetic diversity is concerned to distances and software packages. Finally, the phylogenetic trees and thein types are described with examples of use in practice.
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Association Between Polymorphism of FGF2 and Milk Yield in Cattle
BRZÁKOVÁ, Michaela
The objective of this study is to investigate the effect of polymorphism of the FGF2 gene locus at the milk yield and fertility of Holstein cows. Review contains information about milk yield and reproductive performance of Holstein cows and point out the problem with decreasing fertility of high-producing dairy cows. The second part of review contains information about FGF family, its characterization and its effect of production traits and reproductive traits in Holstein cows. FGF2 was chosen for this study because it is a member of the placental lactogen pathway and interferon- and which means that, FGF2 is included in initiation and maintaining of pregnancy in ruminants and therefore is possible to expect an effect on FGF2 on the milk traits and reproductive traits of cattle. The experimental part of the work deals with the genotyping of 150 bulls of Holstein breed. Genotyping was performed by PCR-RFLP method. Data was obtained and statistically evaluated. No significant effect of SNP11464 FGF2 polymorphism was found with association to milk production of Holstein breed. However, a significant effect of SNP11464 was found in regards to fertility with association to fertility of cows and breeding cattle of the Holstein breed line NXA. This effect was not significant in heifers of both lines and fertility of cows and breading cows in line NEA. SNP11646 FGF2 gene might be useful as a criterion in gene-assisted selection to increase the fertility of Holstein dairy cows but prior to its introduction as a selection criteria in the breeding programme a further investigation of possible effect on fertility is necessary.
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Genetic polymorphism of selected loci in relationship to technological traits of meat
VERNEROVÁ, Kateřina
Within the framework of this diploma, a genotypization of 162 beef cattle of the ČESTR commercial breed from was performed in order to analyze the potential influence of gene coding calpain I (CAPNI) on the resulting beef tenderness. CAPN1 present on the BTA29 chromosome, was selected in previous studies as a candidate gen for QTL. Polymorphism of CAPNI gene was studied using PCR/RFLP method and CAPN530 marker. Genotype identification resulted from this procedure. A 341 bp long fragment was present in homozygotes AA, two fragments of 195 and 146 bp were present in homozygotes GG, and three fragments of 341, 195 and 146 bp were detected in heterozygotes AG. The genotypization output was subsequently statistically evaluated. 11 homozygotes AA, 62 homozygotes GG and 89 heterozygotes AG were detected in the analyzed beef cattle population. More frequent G allele occurred in the set with the frequency of 0,657 and A allele with the frequency of 0,343. Warner-Bratzler shear test was employed to determine beef tenderness based on the shear force attribute. Samples of raw and grilled beef aging 1, 14 and 28 days after the slaughter were analyzed. Statistical methods were used to evaluate relationship between genotype and detected amount of shear force. In case of raw beef, only the samples aging 1 day after the slaughter showed significant difference in the shear force. The most positive results for the given parameter were achieved in case of AG genotype. Samples aging 14 and 28 days after the slaughter showed no difference in shear force and related beef tenderness, indicating no genotype influence. In case of grilled beef, no significant difference in sheer force indicating possible genotype influence was detected at any day of analysis. Genotype influence on grilled beef tenderness within the test animal population is minimal and statistically inconclusive.
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The analyse of polymorphisms of chicken
TYLLEROVÁ, Helena
Chicken growth hormone gene was examined in chosen strains from DOMINANT CZ breeding program - Rhode Island Red (RIR), New Hampshire (NH) and the final hybrids RIRxNH and NHxRIR. Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analyses reveal three MspI restriction sites of intron 1 region. These restriction sites give rise to six RFLP fragments {--} 540 bp, 390 bp, 270 bp, 240 bp, 150 bp, 120 bp. Based on restriction patterns, three alleles of growth hormone were determined. From a possible six genotypes, only four were found in all populations. The allele A1 is the most abundant in RIR strain (0.925), allele A3 is the most abundant in NH strain (0.550). The allele A1 was present in the most of the hybrids.
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