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Study of organic materials thin film homogenity
Lacinová, Eva ; Vala, Martin (referee) ; Zmeškal, Oldřich (advisor)
This thesis deals with study homogeneity of thin organic layers using image analysis. The theoretical parts deal with preparation thin layers and some methods examining their surface, especially optical microscopy and profilometry. Optical microscope NIKON ECLIPSE E200, digital camera NIKON 5400 and computer was used for study homogeneity of organic layers by image analysis. Images of the organic layer and single electrodes, which were steamed on organic layer, were surveyed. Homogeneity of surfaces layers was assessed by errors related with common moments (roughness average, root mean square roughness, skewness, and kurtosis). Differences between single samples in connection with size their common moments and homogeneity are discussed at the close of this work.
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Photobioreactor aeration optimization using image analysis
Hruška, Kryštof ; Létal, Tomáš (referee) ; Naď, Martin (advisor)
This diploma thesis summarizes the knowledge about microalgae, their use, cultivation methods and obstacles that prevent their wider use. In the practical part of the work, a device was designed, constructed, and programmed. This device can analyze the bubbles of the tubular photobioreactor and, based on the obtained data, control its aeration. The Python programming language was used to create the program and the OpenCV library was used to analyze the photographs. The bubble detection is based on the edge detection and the subsequent refinement. The data obtained from the analysis are displayed on the device screen and the data are also stored in a csv file. The discussion lists possible improvements and lessons learned during the creation of this device.
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Microscope and it´s use in engineering practice
Kopecký, Jiří ; Dostál, Lukáš (referee) ; Veselka, František (advisor)
This work deals with the use of the light microscope in industry and its use within the automatization of the diagnostic method specialized on electrical machines with a sliding contact. The method is called the dust particles method and it is used to determine the loss of the brushes used in the machines. The current method involves a manual evaluation of an acquired data, which demands additional tests and the evaluation itself is very difficult. The objective of this work is to simplify and accelerate the method using the computer analysis. First, the usage of the light microscope, through which the samples are obtained, is showed. The method uses these samples as the input. Then, the principles of the dust particles method are demonstrated and the image analysis using the MATLAB program follows. The guide to the image analysis with the elimination of rising defects using the MATLAB program is available. Then, the volume of the intercepted dust particles is calculated using the MATLAB program. The dust particles were obtained from the LFC 55h brush using the Arsenal LPT 1513 – T light microscope.
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Study of Biological Material Attributes by using Image Analysis Methods
Jeřábková, Petra ; Mikula, Milan (referee) ; Zmeškal, Oldřich (advisor)
Within the dissertation thesis “Study of Biological Material Attributes by Using Image Analysis Methods”, attention is focused on monitoring of the application of image analysis methods, mostly a fractal analysis, in studying the properties of various yeast species. The thesis includes determining the number of yeast cells and vegetative propagation of yeast using fractal parameters – fractal measure D and fractal dimension K. Attention is also paid not only to the application of the existing image analysis methods, but also to their renovation. The obtained images were evaluated using the box counting method specified by implementation of wavelet transformation. To monitor yeast cells for a longer time, it was first necessary to prepare a suitable microscopic preparation. To distinguish live and dead cells, the following fluorescent dyes were used: acridine orange, fluorescein diacetate, FUN-1, and Calcofluor White M2R. The images of yeast cells were recorded using a still camera or a CCD camera and microscope. Clips of the same size were obtained from the acquired digital photographs and processed by the HarFA program developed at the Faculty of Chemistry, Brno University of Technology. On the results it is possible to see a change in the fractal dimension depending on time, i.e. on the change of a budding cell structure, or to determine the number and radius of yeast cells upon predefined conditions.
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Using image analysis to determine the number of colonies of microorganisms
Kováčiková, Eva ; Obruča, Stanislav (referee) ; Zmeškal, Oldřich (advisor)
This bachelor thesis focuses on the usage of image analysis in the determination of the quantity and of the size of microbial colonies. Microorganism used in the experiment was Saccharomyces cerevisiae, which forms regular shapes of colonies on the solid medium. The applied methods of image analysis were based on wavelet transform. Fractal analysis of digital images was performed using a computer program HarFA and fractal parameters were defined. The obtained data was used for graph representation of distribution and frequency of colonies.
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Study of thin transparent films properties
Schmiedová, Veronika ; Veselý, Michal (referee) ; Zmeškal, Oldřich (advisor)
The main aim of this bachelor´s thesis is a study of thin transparent layers of organic materials (PPV, P3HT, TiO2, DPP). The theoretical part describe the methods of quality examinations of their surface, mostly the use of optical and interference microscopy. For elaboration of data was used the image analysis via HarFA program. Thickness of layer and the index of refraction was provided by interference microscope. From the interference images was identified the amplitude of groove for various values of treshold, but mainly the interface inhomogenity of groove, which proofs the surface inhomogenity of samples. For the images taken by the optical microscope were allocated the average errors (but also errors of superior sorts) defining the surface inhomogenities of samples. The conlusion of this study compare the results of both methods.
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Analysis of mechanical properties of polymers and polymer compsites from video record of tensile test
Ščudlová, Jolana ; Nezbedová, Eva (referee) ; Žídek, Jan (advisor)
This thesis described mechanical tests with monitoring of camera. The aim of this work is to obtain additional information about material behavior during the test. The mechanical tests were monitored high-definition (HD) digital video-camera and camera with high resolution. The method was applied to the tensile test and Pennsylvania notch test (PENT test). The videorecordings were processed with the assistance image analysis method. The marks were placed on both of these specimens, mutual movement these marks were used to obtain following characteristics. It was Poisson’s ratio and true stress by tensile test. By PENT test it was rate growth crack. The investigated material was elastomeric polyurethane filled by biodegradable polyhydroxybutyrate for tensile test. Second material was polypropylene for PENT test. The camera is adding to the test gives extended information about the material and better interpretation results.
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Evaluation of porosity in Al-alloy die-castings
Straka, Jakub ; Nová, Iva (referee) ; Čech, Jaroslav (advisor)
The purpose of this diploma thesis is an evaluation of die-castings porosity, eventually the evaluation of seats with local squeeze in connection with their mechanical and structural properties. The swatches of alloy AISi9Cu3 were taken from the engine block made by Škoda Auto Company, Mladá Boleslav. To the evaluation and the comparison of the results there were used value of porosity with own measure and other student´s thesis of Brno University of technology. Sets of mechanical and structural properties were selected, evaluated and tested by statistical programs.
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Numerical model of hollow fiber arrangement in heat exchanger
Cabalová, Klára ; Martišek, Dalibor (referee) ; Štarha, Pavel (advisor)
This paper deals with the topic of numerical arrangement of fibers in a heat exchanger. The heat exchanger is scanned in an industrial tomograph and the acquired data are represented by the field of voxels. The method used in this paper is based on tracing the fiber fragments through the use of image analysis and the subsequent numerical connection of the fragments. The result is a set of fibers that are represented by points in the field through which they are passing.
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Study population of bacteria by image analysis methods
Ševčík, Jan ; Veselá, Mária (referee) ; Zmeškal, Oldřich (advisor)
This bachelor thesis is focused on monitoring and application of image analysis methods on Bacillus megaterium and Cupriavidus necator bacteria. The introduction describes a general knowledge about bacteria, microscopic and image analysis methods. At first it was necessary to prepare suitable microscopic specimens using Gram staining for the observation of a bacteria cell. Every picture of bacteria was taken using a CCD camera and a microscope. Box–Counting method was used to identify the optimal threshold value, corresponding to their fractal measure (or surface areas covered with bacteria) and fractal dimension (or entropy of their arrangement on the surface area). After thresholding, the images were further processed using the Mass–Radius method, which defines a distribution of bacteria relative to the centroid of a cluster.
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