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Advanced methods for cardiac cells contour detection
Spíchalová, Barbora ; Čmiel, Vratislav (referee) ; Odstrčilík, Jan (advisor)
This thesis focuses on advanced methods of detecting contours of the cardiac cells and measuring their contraction. The theoretical section describes the types of confocal microscopes, which are used for capturing biological samples. The following chapter is devoted to the methods of cardiac cells segmentation, where we are introduced to the generally applied approaches. The most widely spread methods of segmentation are active contours and mathematical morphology, which are the crucial topics of this thesis. Thanks to the those methods we are able in the visual data to accurately detect required elements and measure their surface chnage in time. Acquired theoretical knowledge leads us to the practical realization of the methods in MATLAB.
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Modeling of action potential of heart cells
Zemánek, Ladislav ; Čmiel, Vratislav (referee) ; Rychtárik, Milan (advisor)
This bachelor’s thesis deals with studies and researches models of action potentials of cardiac cells. It offers a description of the various models including description of their current components. The own solution of this project includes reconstruction of models of cardiac cell in numerical computing environment Matlab and compared with literature.
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Modeling of action potential of heart cells
Zemánek, Ladislav ; Čmiel, Vratislav (referee) ; Rychtárik, Milan (advisor)
This bachelor’s thesis deals with studies and researches models of action potentials of cardiac cells. It offers a description of the various models including description of their current components. The own solution of this project includes reconstruction of models of cardiac cell in numerical computing environment Matlab and compared with literature.
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Advanced methods for cardiac cells contour detection
Spíchalová, Barbora ; Čmiel, Vratislav (referee) ; Odstrčilík, Jan (advisor)
This thesis focuses on advanced methods of detecting contours of the cardiac cells and measuring their contraction. The theoretical section describes the types of confocal microscopes, which are used for capturing biological samples. The following chapter is devoted to the methods of cardiac cells segmentation, where we are introduced to the generally applied approaches. The most widely spread methods of segmentation are active contours and mathematical morphology, which are the crucial topics of this thesis. Thanks to the those methods we are able in the visual data to accurately detect required elements and measure their surface chnage in time. Acquired theoretical knowledge leads us to the practical realization of the methods in MATLAB.
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Physiological consequences of ion concentration changes in transverse-axial tubular system in a model of human ventricular cardiomyocyte
Ohlidalova, D. ; Pásek, Michal ; Šimurda, J. ; Christé, G.
A mathematical model of human ventricular cell incorporating the transverse axial tubular system (TATS) was designed and used to explore the role of TATS in human ventricular cell electrophysiological activity. The model is based on the latest quantitative description of guinea pig ventricular cell electrophysiology and modified to respect the published data obtained from human cardiac preparations. Computer simulations suggest that significant transient depletions of Ca2+ ions and accumulation of K+ ions may occur in the human TATS. The preliminary analysis of the effects of tubular Ca2+depletions on the activity of the model cell indicates their role in limitation of intracellular Ca2+ overload at higher frequencies of stimulation.
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Využití matematického modelu pro odhad frakční plochy tubulární membrány u srdečních komorových buněk.
Pásek, Michal ; Šimurda, J.
The transverse (t-) tubules of cardiac ventricular myocytes create a complex network of membrane invaginations. Many of the key proteins involved in excitation-contraction coupling appear to be located predominantly at the t-tubule membrane. Despite their importance, the fraction of cell membrane within the t-tubules remains unclear: for example, measurement of cell capacitance following detubulation in rat cardiomyocytes suggests 32%, whereas optical measurements suggest up to 65%. We have therefore investigated the factors that may account for this discrepancy. Calculation of the combinations of t-tubule radius, length and density that produce t-tubular membrane fractions of 32% or 56% suggest that the true fraction is at the upper end of this range.
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Quantitative modelling of effect of transverse - axial tubular system on electrical activity of cardiac cells: Development of model II
Pásek, Michal ; Christé, G. ; Šimurda, J.
In this work, we present a new version of cardiac ventricular cell model incorporating the transverse - axial tubular system. The improvements include reformulated description of L-type Ca2+ channel, of Ca2+ induced Ca2+ release from sarcoplasmic reticulum, of intracellular Ca2+ buffering and incorporation of potassium currents Ito, IK(Na) and IK(ATP). In comparison with the previous model (Pásek et al., 2002), the steady state simulations revealed more profound changes of tubular ionic concentration (12.8 % for Ca2+ and 4.7 % for K+ at 1 Hz). The refined model will be used for more exact quantitative exploration of the effect of transverse - axial tubular system on cellular electrical activity and excitation - contraction coupling.
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