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Creating real-time IMS application
Novotný, Filip ; Mácha, Tomáš (referee) ; Nagy, Ľuboš (advisor)
This paper presents a description of the IP Multimedia Subsystem (IMS) architecture and IMS services. IMS is a standardized next-generation networking architectural framework providing advanced services on mobile and fixed networks. The first chapter describes four-layer IMS architecture and also mentions supported services. The second chapter deals with IMS protocols and primarily focuses on the SIP signaling protocol. UDP, RTP and TLS protocols are also included in this chapter. The third chapter is dedicated to practical part of this paper. A VoIP Java application has been created based on the findings gained throughout the thesis. The main part of the created application consists of client-side application, database MySQL and Servlet application for communication between database and client-side application. The whole system was created using SDS Sony Ericsson 4.1. FD1. An OpenIC lite client has been tested during development on our system and results were compared with created client-side application. A web application based on PHP, MySQL and ActionScript then handles administration and monitoring of customers using voice services.
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Influence of surfactants in electroless nickel bath on properties of Ni-P coatings on AZ91 magnesium alloy
Buday, Patrik ; Wasserbauer, Jaromír (referee) ; Buchtík, Martin (advisor)
This bachelor thesis deals with the effect of surfactants on mechanical properties and structure of Ni-P coatings on AZ91 magnesium alloy. The theoretical part of the thesis includes summary of basic knowledge about magnesium alloys, surfactants and electroless deposition of Ni-P coatings, including a detailed description of Ni-P reduction on substrate. The theoretical part is concluded by current research in the field of Ni-P coatings. In the experimental part, morphology of Ni-P coatings was observed by electron microscopy (SEM). Using a light microscope (LM), the effect of surfactants on the deposition rate was observed. By means of energy dispersion analysis, the average phosphorus content of Ni-P coatings was determined. The hardness as a mechanical property of these coatings was determined using a micro hardener.
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IMS based multimedia services
Novotný, Roman ; Nagy, Ľuboš (referee) ; Mácha, Tomáš (advisor)
Submitted work describes IMS (IP Multimedia Subsystem) and focuses on services offered by this technology. In the theoretical part of the thesis is a detailed description of the IMS architecture, in the terms of its components and interfaces. Also a SIP protocol is described as the main signalling protocol, which represents the basis of the IMS architecture and RTP protocol that allows the transmission of multimedia data in real-time. Further, there are provided services that the IMS system brings. The practical part of the work describes the design and implementation of services within the IMS. It is a client-server service. Server part of the system is implemented as a SIP Servlet application. It has access to the MySQL database, which stores records of multimedia files. Client registered in the IMS network can communicate with the SIP servlet sending a request for information on such files. Following this communication, the client can play the file in his online ICP application, or download it into his PC and then play. To play and view multimedia fines is used JMF. The service is implemented in a development environment SDS Ericsson 4.1, which allows simulation of the IMS network.
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Utilization of light scattering techniques in study on protein denaturation
Köbölová, Klaudia ; Kalina, Michal (referee) ; Sedláček, Petr (advisor)
This bachelor thesis is focused on verifying the use of light scattering techniques in the study of protein denaturation. The theoretical part of the thesis describes proteins, their general properties, the process of denaturation and it provides an insight into light scattering. In the experimental part of the study I investigated the denaturation effects of four selected potential denaturing agents – urea, guanidine hydrochloride, sodium dodecyl sulfate, copper (II) sulfate – on two model enzymes: lipase and lysosyme. The denaturation effects of these denaturants were studied with the help of the dynamic light scattering method (DLS). In order to describe the change in particle size during denaturation, I used Z-Average, Volume mean and intensity distribution which were determined by this method.
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Diffusion of dyes in biopolymeric hydrogels
Vyroubal, David ; Mravec, Filip (referee) ; Klučáková, Martina (advisor)
This thesis is focused on diffusion of dyes in biopolymer-based hydrogels. These hydrogels are based on interaction between biopolymer-like electrolytes with oppositely charged surfactants. When polyelectrolytes interact with oppositely charged surfactants, micelle-like nano-containers can be formed. These nano-containers are able of binding hydrophobic compounds. In this study, combination of modified dextran (diethylaminoethyl dextran) with positive charge and oppositely charged sodium dodecylsulphate as surfactant was used for preparation of hydrogels. Next type of hydrogel was based on hyaluronan and positive charged surfactant Septonex (carbethoxypendecinium bromide). As a diffusion probes in hydrogels dyes Nile red and ATTO 488 were used. The diffusion of these dyes from aqeous solutions of NaCl or surfactants into hydrogels was monitored in time. Transport of dyes into structure of hydrogels was characterized by diffusion coefficients and structural parameters of hydrogels.
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IMS aplication creation
Nagy, Ľuboš ; Novotný, Vít (referee) ; Mácha, Tomáš (advisor)
This thesis describes basic principles of IP Multimedia Subsystem, known as the IMS, and shows the possibility for design applications using IMS. Firstly, a generic architecture IMS as layer model divided into four logic layer with definition the interfaces and the main entities is described. The IMS is based on SIP and IP protocols. Then is the architecture of SIP with the main entities explained. Structure of packet, request and response method in the part of SIP is also described. After that, three network services, namely presence services, instant messaging services and push over cellular services are presented. For each services the figuration of their architecture and the basic definition of their main entities are shown. Moreover, the graphical diagrams of SIP signalization are depicted for these services. In the next chapter, the self implementation of services was designed, namely presence services and services for speech transmission with next secondary function as sending email with registration information of user. The project was designed as client - server application in the development studio SDS Ericsson 4.1. The client application was simulated as java application and also as an application for Symbian emulator. The process of client registration was performed with the help of ICP platform. Finally, the diagram of analysis communication client - server is displayed with the captured packets explanation by the help of network analyzer WireShark. The video files, which were specially created for this thesis showing presentations for presence services and sending email. The most important results are summarized in the end of this article.
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Production of polyhydroxyalkanoates from waste substrates and their isolation
Grossová, Marie ; Babák, Libor (referee) ; Márová, Ivana (advisor)
The aim of this work is to study the possibility of microbial production of polyhydroxyalkanoates (PHA). PHA can be used as biodegradable materials. Bacterial strain Cupriavidus necator was used for laboratory production of PHA. This bacterium was cultivated in medium with various precursors to produce copolymers of 3HB with 3HV or 4HB. Another part of the work was aimed at cultivation of C. necator on different waste substrates, especially oils, with the aim to achieve the highest production of polymer. Another large part of the thesis is dedicated to isolation strategies of PHA using enzymes. Commercially used proteases – alcalase and pancreatin – can be used with advantages for digestion of bacterial cells. A number of optimization experiments showed that application of proteases leads to enhancement of PHA purity to about 13%. Purity increase up to 90 % was achieved by adding a surfactant, which promotes the solubility of non-PHA forming polymer. This surfactant increases the purity of 20 % when compared to control. The last part of presented work deals with the use of enzyme solution isolated from Bacillus subtilis medium. Its application to C. necator culture led to the yield of polymer at a purity exceeding 95 %. These results could represent the basis for new isolation strategies, which can lead to more efficient yield of PHA.
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Development of IMS platform applications
Martinek, Pavel ; Nagy, Ľuboš (referee) ; Mácha, Tomáš (advisor)
This master’s thesis is focused on the network architecture of IMS (Internet Protocol Multimedia Subsystem) and development an application for IMS. IMS architecture is the convergence of fixed and mobile networks. IMS enables rapid deployment of new multimedia services. In the first chapter is showed and described architecture IMS in four layers and block model of architecture IMS. Next chapter is focused to protocols, which are used in IMS. One of the most important protocols in network IMS is SIP. This protocol is analyzed in details. For development IMS platform application was used Ericsson SDS (Service Development Studio) in version 4.1. In SDS were created applications type of client and servlet. In this master’s thesis is also used development environment NetBeans. In NetBeans was created application for management of captured images. In the same development environment was developed application that shows the contents of a database. Last chapter is devoted to analyze individual parts of communication between client and servlet, and when client uses http to download image file.
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Fluorescence probes to study the properties of hydrogel systems
Máčala, Jakub ; Venerová, Tereza (referee) ; Mravec, Filip (advisor)
This bachelors thesis is focused on possibility of selected fluorescent probes for study of properties of hydrogel systems. Chosen probes are Prodan for polarity study, HPTS for measurement of pH of the probe environment and platinum complex [2,6-Bis(2-pyridyl)fenyl-C,N,N]chloroplatinum(II) for its unique fluorescence properties and potential of energy transfer studies in hydrogel systems. Spectral properties of fluorophores were defined in selected set of solvents with different polarity with steady state fluorescence spectroscopy method. Properties of HPTS probe were also tested in buffers with different pH. Subsequently, hydrogels based on hyaluronan-septonex and dextran-SDS with incorporated fluorescence probes were prepared. Steady state fluorescence measurement of fluorophores from hydrogel were made using fluorimeter optical fibres. It was found, that probes Prodan and HPTS are suitable for study of polarity and pH of hydrogels and probably they will be able to be used for study of hydrogel with other techniques, like fluorescence microscopy, however platinum complex is, due to the low fluorescence intensity and possible quenching by polar water environment, probably not suitable for other measurements.
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Aggregation Behaviour of Polysaccharides in Aqueous Solutions
Mravec, Filip ; Valko, Marián (referee) ; Hrdlovič, Pavol (referee) ; Valko, Marián (referee) ; Pekař, Miloslav (advisor)
Tato práce je zaměřena na agregační chování nativního a hydrofobně modifikovaného hyaluronanu, v různých molekulových hmotnostech a stupních substituce, ve vodném prostředí. Pro studium bylo vybráno šest fluorescenčních sond s různými vlastnostmi (Pyren; Nilská červeň; Perylen; Akridinová oranž; 6-(p-Toluidino)-2-nafthalenesulfonová kyselina; PRODAN). a výsledky získané pomocí těchto sond byly porovnány s jednoduchým anionaktivním tenzidem (Dodecylsíran sodný). U všech použité sond byly testovány jejich spektrální vlastnosti v závislosti na polaritě okolí a/nebo na koncentraci. Pro stanovení vlastností nepolárního jádra hyaluronového agregátu byly vybrány dvě sondy (Pyren, Nilská červeň). U domén byly sledovány polarita a viskozita vnitřního prostředí a jejich závislost na iontové síle a teplotě. Pro modifikované hyaluronany bylo stanoveno, že jejich kritická agregační koncentrace klesá s rostoucí molekulovou hmotností a stupněm substituce. Pro vlastní doménu platí, že její kompaktnost roste s rostoucí iontovou silou, ale klesá s rostoucí teplotou.
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