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Biocompatible protein cages for encapsulation and internatization of small interfering RNA
Mokrý, Michal ; Balvan, Jan (referee) ; Heger, Zbyněk (advisor)
This thesis is focused on creation of apoferritin nanocarrier with encapsulated small interfering RNA marked with fluorescent dye. Main objectives are optimization of pH and amount of siRNA encapsulated into apoferritin cavity and physicochemical characteristics of created nanocarrier. First part deals with theoretical knowledge necessary for understanding concept of this thesis. Second part describes used methods and evaluated results. Created apoferritin nanocarriers were optimal in size with great hemocompatibility, but long-term stability didn’t meet our expectations.
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Preparation and characterization of magnetic nanoparticles modified by surfactants
Solnička, Ondřej ; Másilko, Jiří (referee) ; Enev, Vojtěch (advisor)
Theme of this bachelors thesis was preparation and characterization of iron(II, III) oxide nanoparticles. Those nanoparticles were modified by surfactants. The surfactants which were used for modifiation were CTAB, TTAB and Septonex. Prepared and modified nanoparticles were taken for characterization by using methods of elemental analysis, thermogravimetry, dynamic light scattering and transmission electron microscopy (TEM). Results from each measurement shows that nanoparticles modified by CTAB had on average 85.9 nm in diameter, TTAB size was on average 108.3 nm and the last nanoparticles with Septonex had 246.6 nm in diameter on average. Prepared modified nanoparticles except the Septonex ones were stable enough to not aggregate. Septonex modified nanoparticles had greater tendenty to aggregate.
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The issue of the use of carbon nanotubes in cement composites
Pacltová, Klára ; Hela, Rudolf (referee) ; Bodnárová, Lenka (advisor)
The bachelor thesis deals with the analysis of the efficiency of carbon nanoparticles in cement composites. The theoretical part of the bachelor thesis is focused on the research of information about carbon nanotubes and their influence on cement composites. The practical part follows the research of the theoretical part, where is verified the effectiveness of carbon nanotubes in cement composites at different dosages.
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Application of nanofiller in electroinsulating materials
Mohyla, Petr ; Rozsívalová, Zdenka (referee) ; Frk, Martin (advisor)
This project deals with calculation parts of complex permittivity varnish samples which is filled by nanoparticles of metal oxides. Opening of this project deals with theory of dielectric and effects in dielectric, theory of compounds and expressions in composite systems. Following topic is theory of nanoparticles and nanotechnologies and summarize adjusted results about this branch of knowledge. In practical part of work is include method which was use to make varnish samples filled by nanoparticles, prepare to measurement methods and whole results of measurement. In the end of work is analysis influence of filler particles each filler types to dielectric material and results verified mixture mathematical expressions.
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The effect of therapeutical ultrasound and metalic nanoparticles on carcinoma cells
Kalužová, Adéla ; Rozman, Jiří (referee) ; Bernard, Vladan (advisor)
This thesis focuses on the research of an effect of ultrasound and nanoparticles on cancer cells. In the literature search there the applications of ultrasound field in the interaction with aplication of metallic nanoparticles to viability of cancer cells are described, on the basis of in vitro experiments‘ performance. The purpose of metallic nanoparticles‘ application should be a possible reinforcing of its antitumor effect thanks to the presence of ultrasound field. In next part of the literature search there are included the technical performance of devices, their indications and contraindications, parameters of ultrasound fields and application of metallic nanoparticles. The metallic nanoparticles have been becoming a part of the human’s life more and more, that is why it’s necessary to deal with these miniature particles at a higher level. The aim of this thesis is to suggest the ways of resolution based on the principle of sounding of cells with the ultrasound field of suitable parameters and in combination with the application of metallic nanoparticles with a successive performance of the cell viability tests. The viability was determinated by the MTT assay test. Another part of this work is the measurement of parameters of this zone by means of a submersible needle hydrophone, for a better understanding of the ultrasonic zone. In the final part of this work there is necessary the statistical evaluation of the measured data of the experiments carried out and the discussion of the results obtained.
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Preparation of graphene quantum dots and study of their properties
Zdražil, Lukáš ; Mikula, Milan (referee) ; Zmeškal, Oldřich (advisor)
Current methods for preparing graphene quantum dots (GQDs) rely on oxidation and reducing agents or require energy-intensive and technologically demanding preconditions. Applying microwave expansion and liquid phase exfoliation (LPE) in a sample of graphite powder enabled us to prepare GQDs that exhibit strong luminescence in the blue region of the visible spectrum. The proposed technique for synthesizing GQDs is energetically undemanding and does not necessitate additional chemical components.
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Reflex Treatment of Transparent Materials
Meluzín, Ondřej ; Tulka, Jaromír (referee) ; Molliková, Eva (advisor)
This thesis deals with the possibilities and methods of coating of transparent materials in order to improve their optical-reflective properties. It describes various methods that nowadays coatings can be applied on an industrial scale, while maintaining a constant thickness and properties across the applied coating. Further provides specific examples of how the application of different optical coatings can improve the desired properties of materials used for technical applications.
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Molecular Communication in Nano-networks Utilizing Diffusion Process
Kupka, Lukáš ; Mašek, Pavel (referee) ; Hošek, Jiří (advisor)
The bachelor thesis is focused on theoretical desription of diffusion-based molecular communication in nanonetworks, description of simulation framework N3Sim and building a model of nanocommunication in this simulator. Second part of thesis is focused on molecular communication based on electromagnetic waves. In this case simulator NS-3 and Nano-Sim module has been used. Practical part contains detail analysis of simulators adjustment and results of simulations. Simulations are performed with different settings in several scenarios. Their comparison and results is summarized in conclusion.
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PEGylated micelles for diagnostic and therapy against solid tumors
Hoang, Stéphane ; Hrubý, Martin (advisor) ; Frochot, Céline (referee) ; Bianco, Alberto (referee)
Title : PEGylated micelles for diagnostic and therapy against solid tumors. Keywords : micelles, ferrocene, photolysis, vectorization, nanoparticle, singlet oxygen Abstract : Nanomedicine is an emerging field that addresses the challenges faced by conventional medicine. The use of nanometric objects makes it possible to reduce side effects through targeted, specific treatment at reduced doses. Among these nanoparticles, micelles are widely exploited thanks to their small size and high modularity. As a result, their application extends to many fields, including oncology. Indeed, their size enables them to take full advantage of the characteristics of the tumor microenvironment, resulting in increased accumulation and retention of micelles in tumor tissue. Thus, the use of micelles as nanovectors offers a theranostic approach to cancer therapy. In this context, the work presented here is divided into two distinct parts. The first presents the synthesis and formulation of photodegradable PEGylated micelles capable of both generating cytotoxicity and delivering a hydrophobic molecule intracellularly. The second part focuses on the enhancement of the EPR effect by sonoporation, a method considered to be non-invasive. Results on murine models demonstrated the positive effect of this method by greatly improving...
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Preparation and characterization of magnetic nanoparticles modified by surfactants
Solnička, Ondřej ; Másilko, Jiří (referee) ; Enev, Vojtěch (advisor)
Theme of this bachelors thesis was preparation and characterization of iron(II, III) oxide nanoparticles. Those nanoparticles were modified by surfactants. The surfactants which were used for modifiation were CTAB, TTAB and Septonex. Prepared and modified nanoparticles were taken for characterization by using methods of elemental analysis, thermogravimetry, dynamic light scattering and transmission electron microscopy (TEM). Results from each measurement shows that nanoparticles modified by CTAB had on average 85.9 nm in diameter, TTAB size was on average 108.3 nm and the last nanoparticles with Septonex had 246.6 nm in diameter on average. Prepared modified nanoparticles except the Septonex ones were stable enough to not aggregate. Septonex modified nanoparticles had greater tendenty to aggregate.
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