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Identification of microorganisms in cosmetic products with probiotics
Langová, Denisa ; Španová, Alena (referee) ; Trachtová, Štěpánka (advisor)
Probiotics products are an integral part of the current market. Products containing probiotics cultures are also cosmetic products. The first part of the study focuses on testing of bacterial survival abilities in the environment of preservatives presented in cosmetic products. Collection strains of genus Lactobacillus were used for these tests. Another part of the study focuses on isolation of bacterial DNA from probiotic cosmetic products Ryor, Yoghurt of Bulgaria, FeminaMed and Lactovit Activit in PCR-ready quality. DNA was isolated by fenol extraction and with magnetic particles. Presence of bacteria was proved by genus and species specific PCRs Lactobacillus. Species specific PCR for identification of Lactobacillus pentosus was optimalized. Species identification was in accord with data declared by producers.
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Study of the effect of cosmetics on the human skin microbiome using molecular techniques
Alexová, Adéla ; Kovalčík, Adriána (referee) ; Němcová, Andrea (advisor)
The theoretical part of the thesis is focused on the basic description of the physiology of the skin, human microbiome and a brief summary of where individual microorganisms occur. Furthermore, there is a list of analytical and microbiological methods that are used in this thesis. In the beginning, the practical part is focused on determination of antimicrobial effects of the chosen cosmetic products using microbial tests. Then, the inhibiton and microbial effect of the chosen cosmetic products on examined microorganisms has been measured using ELISA method. The second part of the thesis is focused on the isolation of bacterial DNA in quality that would be high enough to be used for amplification in PCR. There has been an optimalization of isolation of microbial DNA that was to be found on tested subjects’ skin. The presence of chosen microorganisms on skin before and after the usage of cosmetic products was measured using a PCR method. PCR products were then detected using gel electrophoresis. From the gathered data it is clear that the number of observed microorganisms has changed significantly after the application of cosmetic products.
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Development of methods for determination of authenticity of cosmetic products with a plant component
Langová, Denisa ; Brázda, Václav (referee) ; Vorlová, Lenka (referee) ; Márová, Ivana (advisor)
In the final thesis, methods were developed for determining the authenticity of cosmetic products and food products with a plant component. The presence of natural ingredients in a series of model and commercial cosmetic products was verified and their authenticity was verified, too. The focus of the work was the development of a molecular-biological method for the detection of plant components (herbs, fruits, essential oils) and probiotics present in cosmetic products. Commercially available isolation kits were used for the isolation of DNA from cosmetic products and the method was optimized for the isolation of plant DNA present in cosmetic products. The presence of a plant matrix in cosmetic products was detected by the polymerase chain reaction (PCR) method. Instrumental methods were used to detect the presence of fragrance allergens in cosmetic products. A method of quantifying these allergens using liquid chromatography was introduced and the measurement results were compared with the results of gas chromatography with mass detection. The proposed method of isolation and subsequent amplification of plant DNA is suitable for determining the authenticity of herbal ingredients in cosmetic products. The proposed HPLC method is a simple and inexpensive method suitable for determining the allergens present in the amount necessary to comply with EU analyte legislation and in the required sensitivity (except for hydroxycitronellal). Both methods are suitable to be used for the analysis of food products, too.
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Identification of probiotics in cometic product using PCR technique
Akmalova, Adelina ; Langová, Denisa (referee) ; Smetana, Jan (advisor)
This bachelor thesis deals with the identification of probiotic microorganisms in cosmetic products by polymerase chain reaction (PCR). The aim of this work was to confirm the presence of bacterial DNA in cosmetic products containing probiotic microorganisms. The theoretical part of the thesis focuses on the role of probiotic microorganisms in maintaining skin health and their effects on the human body. Different methods for the identification of probiotics are also highlighted with a focus on molecular genetic approaches. In the experimental part of the work, two cosmetic products with declared presence of probiotic microorganisms were tested. Two methods were used to extract DNA from the products: phenol extraction and commercial kit method. Furthermore, amplification of a specific region of the 16S rRNA gene was performed by PCR. The PCR products were subjected to agarose gel electrophoresis and visualized by ultraviolet light. All cosmetic products showed the presence of the Bacteria domain and the genus Lactobacillus. In addition, species-specific PCR confirmed the presence of the declared probiotic species Lactobacillus pentosus and Lactobacillus bulgaricus in the products.
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Study of the effect of cosmetics on the human skin microbiome using molecular techniques
Alexová, Adéla ; Kovalčík, Adriána (referee) ; Němcová, Andrea (advisor)
The theoretical part of the thesis is focused on the basic description of the physiology of the skin, human microbiome and a brief summary of where individual microorganisms occur. Furthermore, there is a list of analytical and microbiological methods that are used in this thesis. In the beginning, the practical part is focused on determination of antimicrobial effects of the chosen cosmetic products using microbial tests. Then, the inhibiton and microbial effect of the chosen cosmetic products on examined microorganisms has been measured using ELISA method. The second part of the thesis is focused on the isolation of bacterial DNA in quality that would be high enough to be used for amplification in PCR. There has been an optimalization of isolation of microbial DNA that was to be found on tested subjects’ skin. The presence of chosen microorganisms on skin before and after the usage of cosmetic products was measured using a PCR method. PCR products were then detected using gel electrophoresis. From the gathered data it is clear that the number of observed microorganisms has changed significantly after the application of cosmetic products.
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Influencer marketing v oblasti kosmetických výrobků
Procházková, Karla
The bachelor thesis deals with influencer marketing in the field of cosmetic products and its influence on consumers. The literary part introduces the reader to influencer marketing and its use. The practical part represents a questionnaire survey that examines whether customers are affected by this kind of marketing.
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Endocrine disruptors in consumer goods
Pařízková, Alžběta ; Šuta, Miroslav (advisor) ; Cajthaml, Tomáš (referee)
The Thesis focuses on the group of specific organic compounds with a potential risk of their endocrine disruptive effect, and appearing as UV filters in various cosmetic products. An extended list of such products was created on the basis of search in drugstores, pharmacies and perfume shops. The products were further grouped as cosmetics for adults, children, and those declared as bio products. The source data collection counted 156 individual products, with origin in 40 drugstores, 20 pharmacies, and 10 perfume shops. The data collection sites were distributed in big cities, their suburb parts, shopping parks, and small towns on many places in the Czech Republic. The products were mostly sun protection creams, oils and emulsions, body creams and emulsions, lips balsams, and hair cosmetics. In these items an occurrence of 7 organic compounds (suspected endocrine disruptors) from the SIN List, used as parts of UV filters, was followed. The compounds of interest were: ethylhexyl methoxycinnamate; benzophenone-1, benzophenone-2, benzophenone-3; 4,4-dihydroxyphenone; 4-methylbenzylidene camphor; 3-benzylidene camphor. Alternative names, product brand names, chemical structures, CAS numbers etc. were also collected from various sources. Of this group only ethylhexyl methoxycinnamate and benzophenone-3...
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Identification of microorganisms in cosmetic products with probiotics
Langová, Denisa ; Španová, Alena (referee) ; Trachtová, Štěpánka (advisor)
Probiotics products are an integral part of the current market. Products containing probiotics cultures are also cosmetic products. The first part of the study focuses on testing of bacterial survival abilities in the environment of preservatives presented in cosmetic products. Collection strains of genus Lactobacillus were used for these tests. Another part of the study focuses on isolation of bacterial DNA from probiotic cosmetic products Ryor, Yoghurt of Bulgaria, FeminaMed and Lactovit Activit in PCR-ready quality. DNA was isolated by fenol extraction and with magnetic particles. Presence of bacteria was proved by genus and species specific PCRs Lactobacillus. Species specific PCR for identification of Lactobacillus pentosus was optimalized. Species identification was in accord with data declared by producers.
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Organization of production and quality in the company
KISSOVÁ, Beáta
The aim of this thesis, which is called "Organization of production and quality in the company" was to assess how the management and organization help to abide by the production quality. There was introduced a production process of two chosen products, a process of the production flow and a background of the production in the selected company. Next part includes the description of the quality management of the chosen products including requirements and standards which are necessary for the production of the cosmetic products. Based on the findings of deficiencies, there were suggested measures to improve the quality management system.
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Loyalty system of Lancôme Beauty Institute
Nováková, Šárka ; Zamazalová, Marcela (advisor) ; Marádová, Lucie (referee)
The aim of this paper is to evaluate the loyalty system of Lancôme Beauty Institute and ascertain with the use of questionnaire investigation the satisfaction of customers with offered benefits and alternatively suggest the possibilities of improvement of the system. The theoretical part is focused on the general definition of customer satisfaction. The important part is recruitment, maintaining and motivation of current and potential customers. The practical part describes the functioning of the loyalty system of Lancôme Beauty Institute, the stages of development and implications of changes in the loyalty system. The questionnaire investigation was used to ascertain the satisfaction with loyalty system as well as to find the main imperfections -- the data were gathered, classified and analysed.
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