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The differences in the virome of different populations of honey bee (Apis mellifera)
Kadlečková, Dominika ; Tachezy, Ruth (advisor) ; Votýpka, Jan (referee)
European honey bee (Apis mellifera) is major pollinator for agriculture and vital for food production. Large number of viruses infecting A. mellifera have been discovered over the years, but it isn't yet known if they are pathogenic for their host. However, presence of non-viral pathogens like Varroa destructor can greatly increase their virulence and have fatal consequences for the colony. The aim of this study was to test and verify robustness of the method for virome detection on healthy honey bees from the Czech Republic. Last but not least we aimed to detect non-viral parasites and correlate their presence with detected viruses. We have successfully identified large number of viral sequences from different viral families. Viral composition was found to be influenced mainly by colony from where the honey bees were collected. That was mainly given by a large amount of bacteriophages in the samples. However, analysis of individual viruses, known to infect honey bee, indicated that viral prevalence and viral loads of specific viruses is quite different among individual honey bees from the same colony. Interestingly we were able to find highly diverse Lake Sinai viruses. We were able to observe correlations either between individual viruses or viral other non-viral pathogens. Further analysis is...
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Beekeeping Exposition of the Prague Botanical Garden and its Application in the Process of Education
Fialová, Alžběta ; Skýbová, Jana (advisor) ; Hanel, Lubomír (referee)
Beekeeping Exposition of the Prague Botanical Garden and its Application in the Process of Education This bachelor thesis deals with the topic of a beekeeping exposition of the Prague Botanical Garden currently in a phase of planning. It is divided in two parts. In the first part, the honey bee (Apis mellifera) is described - its anatomy, ethology, diseases and bee products. In the last chapter of the theoretical part an example of a possible use of a traditional artificial hive in primary school education is introduced. The second part is dedicated to the beekeeping exposition itself and includes a questionnaire survey, the aim of which is to find out to what extent the topic of the honey bee is dealt with in the curriculum; whether schools organize any events related to the topic such as discussion forums or educational excursions to eco-centres; or whether schools would be interested in the planned exposition as well as available materials such as worksheets. Furthermore, the planned exposition is described and its possible application in the process of education is suggested. Later, significant plants suitable for beekeeping, which had been mentioned in the description of the exposition proposal, are thoroughly described in a well-arranged chart. At the end of the thesis, a worksheet for lower...
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The differences in the virome of different populations of honey bee (Apis mellifera)
Kadlečková, Dominika ; Tachezy, Ruth (advisor) ; Votýpka, Jan (referee)
European honey bee (Apis mellifera) is major pollinator for agriculture and vital for food production. Large number of viruses infecting A. mellifera have been discovered over the years, but it isn't yet known if they are pathogenic for their host. However, presence of non-viral pathogens like Varroa destructor can greatly increase their virulence and have fatal consequences for the colony. The aim of this study was to test and verify robustness of the method for virome detection on healthy honey bees from the Czech Republic. Last but not least we aimed to detect non-viral parasites and correlate their presence with detected viruses. We have successfully identified large number of viral sequences from different viral families. Viral composition was found to be influenced mainly by colony from where the honey bees were collected. That was mainly given by a large amount of bacteriophages in the samples. However, analysis of individual viruses, known to infect honey bee, indicated that viral prevalence and viral loads of specific viruses is quite different among individual honey bees from the same colony. Interestingly we were able to find highly diverse Lake Sinai viruses. We were able to observe correlations either between individual viruses or viral other non-viral pathogens. Further analysis is...
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Device for Honey Bee Activity Measurement
Fučík, Jan ; Šimíček, Tomáš (referee) ; Novák, Marek (advisor)
This thesis engages in characteristic honey bee's behaviour first. Subsequently it engages in project of device for honey bee activity measurement. It reasons about non-electric quantities which can honey bee's behaviour represent. Description of components which can be use for measure these quantities follows. Description is intent on possible accuracy and energy consumption of each component. Appropriate method is selected subsequnetly. In realitionship with method are selected sensors and projected controlling circuit. There is a summary of parameters of device in resume. There is also design of the parts which do not work as good as is ideal in resume.
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Bee nuc creation and proliferation of bee colonies
NOVOTNÝ, Josef
The aim of this thesis is to thoroughly summarize the ways of honey bees' reproduction, especially the means of splits creation. Additionally, the thesis provides a description of the care necessary to develop the splits into a sufficient number of bee colonies prepared for hibernation.
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The most common diseases of bees keeped in Czech Republic
DURČANSKÝ, Pavel
This thesis deals with the most common disease occurring in bees kept in the Czech Republic. It focuses mainly on the Varroa destructor mite. Within individual diseases and foetus as well as adult-bee infections, I have observed the causes of occurrence of non-infectious diseases, their progress and possible ways of treatment. Furthermore, I describe infections that can be of viral, bacterial, fungal, and parasitic origin. Here I deal with the description of the originator, clinical symptoms, progress of the disease, spreading, ways of treatment and possible preventative measures.
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Honey bee (Apis mellifera) virome
Kadlečková, Dominika ; Tachezy, Ruth (advisor) ; Rothová, Olga (referee)
Microbiome is composed of all microorganism (fungi, bacteria, archea, protozoa, virus) sharing the same space at the same time. Virome which consists of viruses either of the host or viruses of other microorganisms and of genetic elements derived from viruses, is part of the microbiome. Microbiome plays a role in a number of processes which are vital for development and survival of the host. Virome can affect host either directly or indirectly by interaction with other parts of microbiome. This thesis is focused on virome of Apis mellifera, globally managed pollinator. Viral diseases are one of the factors responsible for high colony losses of this pollinator and therefore this work is mostly dedicated to those viruses which were confirmed as pathogens of Apis mellifera. Also, their interaction with other pathogens of Apis mellifera, which leads to great losses of these pollinators will be discussed. Key words: virome, honey bee, Apis mellifera, honey bee viruses, colony losses
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Device for Honey Bee Activity Measurement
Fučík, Jan ; Šimíček, Tomáš (referee) ; Novák, Marek (advisor)
This thesis engages in characteristic honey bee's behaviour first. Subsequently it engages in project of device for honey bee activity measurement. It reasons about non-electric quantities which can honey bee's behaviour represent. Description of components which can be use for measure these quantities follows. Description is intent on possible accuracy and energy consumption of each component. Appropriate method is selected subsequnetly. In realitionship with method are selected sensors and projected controlling circuit. There is a summary of parameters of device in resume. There is also design of the parts which do not work as good as is ideal in resume.
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Telomere length compensation mechanisms as players in longevity and stress adaptation of insects
SÁBOVÁ, Michala
Telomeres are nucleoprotein structures at the ends of eukaryotic chromosomes that are important for genome stability and integrity. They are shortened with each cell cycle and during organismal aging. Although the most common telomere length compensation mechanism is the activity of a special reverse transcriptase, telomerase, in Drosophila telomeres are maintained by the retrotransposition of telomeric elements. In mammals, telomere length and telomerase activity can be influenced by lifestyle and the environmental conditions. This thesis is focused on activity of telomere length maintenance mechanism in insects in relation to aging and stress response.
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