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Toxicity of microplastics and nanoplastics
Nechvátal, Marek ; Klouda, Karel
The article summarizes the knowledge about the influence of micro and nanoplastics on environmental components. Attention is paid to a complex set of the main toxic effects of plastic particles on selected groups of environmental organisms, especially new information and interactions with living organisms that have the potential to cause ecotoxicological damage.
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Toxicity of nanotextiles as respirators waste to Daphnia Magna
Slaný, Jan ; Roupcová, Petra ; Pavlovský, Jiří ; Klouda, Karel
In recent years, the prevalence of nanotubes has increased due to the covid-19 disease situation worldwide. Nanotextiles are used in the form of face masks and respirators, which are a source of environmental pollution by nanoparticles. The aim of this paper is to perform ecotoxicological tests according to European standards and Czech legislation. First, non-woven fabrics are briefly described and a small planktonic crustacean (Daphnia magna S.) is introduced. The second part focuses on the experiments themselves and their results. In all cases, the nanotextiles tested did not have a toxic effect on the organisms, but a reaction could be observed. Further tests will be carried out to explain and clarify the effects.
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Study of main mycoremediation aspects - effect of bioavailability, biodegradation and toxicity of organic pollutants
Čvančarová, Monika ; Cajthaml, Tomáš (advisor) ; Kočí, Vladimír (referee) ; Bosáková, Zuzana (referee)
Many organic compounds are released to the environment and can be harmful to living organisms. These compounds are often persistent and toxic. Some are mutagens, carcinogens, endocrine disruptors or they can cause an increase in bacterial resistance. They tend to accumulate in nature and their transformation is a long-term process. Therefore, various remediation techniques are needed for decontamination. Remediation and bioremediation processes depend on many factors which should be critically evaluated. This dissertation thesis studies the relationship between bioavailability, biodegradation and toxicity of polychlorinated biphenyls, polycyclic aromatic hydrocarbons and fluoroquinolone antibiotics. These compounds of different origin, character and properties were degradated by ligninolytic fungi. Desorption behaviour of pollutants from historically contaminated sites, degradation potential of ligninolytic fungi, ongoing degradation mechanisms, transformation products and their toxicity were studied as important factors for evaluation of mycoremediation and its environmental impact. The results show that determination of bioaccessible fraction by sequential supercritical fluid extraction is very useful for precise prediction of biodegradability of pollutants. The evidence that ecotoxicity and...
Possibilities of using the multigenerational test on the organism Daphnia magna
Měšťánková, Anna ; Maršálková, Eliška (referee) ; Zlámalová Gargošová, Helena (advisor)
This bachelor thesis focuses on the study of the effects of pharmaceutical substances ibuprofen, ketoprofen, and acetylsalicylic acid, as well as their combinations, on the freshwater organism Daphnia magna through multigenerational ecotoxicological tests. The presence of pharmaceutical residues in ecosystems is a significant environmental problem, which necessitates a deeper understanding of their long-term effects on organisms, including aquatic ones. The work monitored the influence of the aforementioned pharmaceuticals through acute tests, as well as chronic ones. While the acute toxicity of these substances is not significant, their long-term impact was demonstrated through chronic tests. A significant decrease in the reproductive capacity of D. magna was observed, manifesting not only in the delay of the first brood but primarily in the overall decrease in offspring numbers across generations. The findings indicate that the combination of drugs can have synergistic toxic effects, which in some cases may lead to a complete cessation of reproduction. Additionally, growth inhibitions, behavioral changes, and body deformations in exposed organisms were observed. These multigenerational tests provide a comprehensive and realistic view of the potential ecotoxicological risk of pharmaceuticals in freshwater ecosystems and support the need for in-depth research on the impact of pharmaceuticals on aquatic organisms.
Evaluation of ecotoxicity of selected chemicals with using algal test.
Kosárová, Hedvika ; Pavlovský, Jiří (referee) ; Doležalová Weissmannová, Helena (advisor)
This bachelor thesis deals with the ecotoxicity of 2 - [(2,6-dichlorophenyl)aniline] benzene acetic acid (diclofenac), ?-methyl-4-(2-methylpropyl) benzene acetic acid (ibuprofen) and mixture of cadmium and diclofenac and studies the above using algal toxicity test in accordance with ISO 8692. The theoretical part describes the issue of pharmaceuticals in the environment, and characteristics of standards algae tests and alternative tests. Freshwater green algae Desmodesmus subspicatus were used as testing organisms in all the implemented algal toxicity tests. By the means of these tests, the IrC50 values for the standard substance potassium dichromate, the drugs diclofenac and ibuprofen and cadmium were determined. The toxicity of cadmium in mixture with diclofenac was also evaluated.
Ecotoxicity of selected musk compounds
Cvikýřová, Zuzana ; Vávrová, Milada (referee) ; Zlámalová Gargošová, Helena (advisor)
Products of daily use, which are mainly cosmetics and personal care products could significantly affect the environment. Recently, in terms of environmental protection, organic compounds responsible for the pleasant smell of these products - synthetic fragrances or musk compounds - are often mentioned. This diploma thesis aims to assess the ecotoxicity of several new substances from the group linear (alicyclic) musk compounds. From this group following fragrance ingrediens were tested: lilial, linalool, arocete and aroflorone. Since the majority of their residues enter the aquatic ecosystem, aquatic freshwater crustacean Daphnia magna and Thamnocephalus platyurus and aquatic plant Lemna minor were selected as test organisms. Individual ecotoxicity of above mentioned substances was evaluated as well as toxicity of binary mixture of arofloron and linalool on organisms Daphnia magna and Thamnocephalus platyurus. Based on the test results the ecotoxicological values LC50, EC50 and IC50 for the tested substances were evaluated and their ecotoxicity was compared. Lilial showed the highest ecotoxicity. Value of 24h EC50 for organism Daphnia magna was 2.13 mg/l and value of 24h LC50 for the organism Thamnocephalus platyurus was 10.86 mg/l.
Ecotoxicological evaluation of the sludges from water treatment plants
Hellingerová, Lucie ; Dvořáková,, Dagmar (referee) ; Zlámalová Gargošová, Helena (advisor)
Evaluation of the sewage sludges from different sewage water treatments will be performed by means of available ecotoxicity tests. The evaluation will be done in accordance with the valid legislation. On the basis of the obtained values the sludges will be classified into the respective grades of leachability and the dangerous property of the sewage sludges ecotoxicity will be evaluated.
Ecotoxicological evalution of agents based surfactants.
Doležalová, Šárka ; Vávrová, Milada (referee) ; Zlámalová Gargošová, Helena (advisor)
The surfactants have become a common part of everyday human life. For their properties they are used as aditive components of foam extinguishers used in large quantities for area fires fighting. This thesis deals with the ecotoxicological evaluation of commercially available fire-fighting agents Sthamex F 15, Moussol APS F 15, Finiflam F 15, Expyrol F 15 and Pyrocool B, which are made on the basis of surfactants. In the framework of this thesis the ecotoxicological effects of selected extinquishing agents were assessed through aquatic ecotoxicity tests with organisms Thamnocephalus platyurus, Daphnia magna, dicotyledonous plants Lemna minor and white mustard seeds Sinapis alba. Via tests of contaminated soils leachate the effect of soil sorption complex on the ecotoxicological effects was evaluated. Extinguishing agents were further considered in terms of acute ecotoxicity for soil organism via ecotoxicological tests in the contact arrangement using earthworm Eisenia fetida and lettuce seeds Lactuca sativa. To assess the influence of biodegradation under different conditions on ecotoxicological effects the seed germination tests of L. sativa were conducted with hindsight. According to the results of the individual tests this work discuss the need for further evaluation of the negative influence of selected agents, while paying special attention to legislative measures.
The problems of interaction testing compounds in ecotoxicology.
Pavlíčková, Ivana ; Pavlovský, Jiří (referee) ; Doležalová Weissmannová, Helena (advisor)
Bachelor thesis summarizes current knowledges of the problems of interaction of tested compounds and the determination of ecotoxicity. The work deals with the evaluation of ecotoxicity of binary mixtures of substances, toxic metals, or biologically active substances. The work is a brief summary of ecotoxicity tests used.
Influence of advanced oxidative processess on ecotoxicity of pesticides
Goma, Bernadette ; Procházková, Petra (referee) ; Zlámalová Gargošová, Helena (advisor)
The topic of this bachelor thesis is a study of the influence of advanced oxidation processes on the ecotoxicity of pesticides Metazachlor and Chloridazon. Both herbicides are commonly used to protect crops such as oilseed rape, turnips, chard or fodder beet against whole range of undesirable monocotyledonous and dicotyledonous plants. Application is most often on large areas and therefore pose a high risk to the quality of the environment. The possible influence on the representatives of producers and consumers of the aquatic ecosystem was monitored in the work. Solutions of pesticides, which were subjected to selected methods of treatment using advanced oxidation processes, were tested on aquatic organisms, specifically on crustaceans Daphnia magna and aquatic plant Lemna minor. From the results of individual tests, the ecotoxicological values of EC50 and IC50 were calculated and their ecotoxicity and thus the efficiency of oxidation processes in the removal of pollutants from wastewater were compared.

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