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Use of antimicrobial products of animal origin in cosmetics
Puškárová, Radka ; Bokrová, Jitka (referee) ; Márová, Ivana (advisor)
The Bachelor thesis deals with characterization a whey in free and encapsulated form and its application to cosmetic industry. The theoretical part is focused on characterization of whey, especially on its antimicrobial properties, and use of this effect in selected cosmetics preparatives. Further, methods of encapsulation and particles characterization were described. In the experimental part, antimicrobial properties of whey were tested by two different methods. Whey was encapsulated into three different types of particles: liposomes, alginate and chitosan. Long-term colloid stability of particles during one and three weeks of storage in model conditions was tested. Further, particles were added into model skin creams. Long-term sedimentation stability of these emulsions was analyzed by analytical centrifuge.
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Sales Promotion and Advertising of Company
Liška, Marek ; Bednářová, Markéta (referee) ; Mráček, Pavel (advisor)
The main goal of my work is based on the analysis to propose appropriate changes / additions to marketing communication tools of a particular company, focusing mainly on sales promotion tools and advertising. The implementation of the proposals should then increase the awareness of the company, gain new customers or increase the satisfaction (and thus re-visit) of existing customers. I also want to focus on the start-up and subsequent operation of the company in connection with the pandemic situation and the resulting crisis. The first theoretical part is focused on defining individual economic concepts, methods and tools of communication. The second practical part is mainly based on the method of analysis of the current state of the company and the competition. In this part, I also focus mainly on the communication tools used by the salon, on the target group of customers, and the overall evaluation.
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Assessment of chosen aromatic compounds in cosmetics
Novotná, Petra ; Souralová Popelková, Miriam (referee) ; Vítová, Eva (advisor)
This master thesis deals with the assessment of selected fragrant substances in cosmetic products. At the beginning the occurrence, methods of acquiring and application of these substances is described. They are essential components primarily in cosmetic products, however they can cause an allergic reaction in sensitive individuals. The theoretical part of this thesis also describes the cause and the progress of these undesirable effects. There are several hundreds, up to thousands fragrant substances used in the perfume industry, from which 26 was designated by the European regulations as potential allergenic fragrant substances and their content is regulated. Legislative standards as well as the overview of the methods used to determine these fragrances are given here. The experimental part is focused on the development and validation of the separation methods for determining of the selected and potentially allergenic fragrant substances. The method used was a solid phase microextraction using Carboxen/Polydimethylsiloxane fiber combined with a gas chromatography coupled to a flame ionization detector (SPME-GC-FID). Amylcinnamyl alcohol, benzyl alcohol, cinnamyl alcohol, coumarin, lyral, ?-isomethyl ionone were among the determined allergenic substance. Consequently, this method was also applied on 10 selected cosmetic products specimens, in which the linalool and geraniol were identified as the most common allergenic substances.
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Humic acids in medicine and cosmetics
Šmídová, Helena ; Sýkorová,, Ivana (referee) ; Pekař, Miloslav (advisor)
The main concern of this study was to form a web and literature search focused on the known theoretical knowledge, patent application and the actual realization, for the use of humic acids or material containing humic acids in human and veterinary medicine and cosmetics. The output is also a proposal to be promising directions in exploring these areas. The introduction deals with humic substances in general with emphasis on humic acids, history of humic chemistry and structure of humic substances. The second part deals with medical aspects, pharmacological effects and the use of humic substances in human medicine, veterinary medicine and cosmetics, also takes account of humic substances from the perspective of the environment. The task of third experimental part was to learn the basics of isolation and characterization of humic acids (measurement of IR and UV / VIS spectra and determination of moisture and ash in samples of humic acids).
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Less conventional botanical sources of cosmetic raw materials
Novotná, Petra ; Vespalcová, Milena (referee) ; Zemanová, Jana (advisor)
The aim of this bachelor’s thesis is an investigation of use of less traditional plant sources in cosmetic industry. This review is focused on black elder (Sambucus nigra), sea buckthorn (Hippophae rhamnoides), quince (Cydonia oblonga), dog rose (Rosa canina), and stinging nettle (Urtica dioica). The substances Isolated from these plants can be used in various cosmetic products. The first section of this search deals with history and botanical characterization of these plants. The next section deals with significant substances contained in these plants and their use not only in cosmetic industry.
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Preparation of encapsulated enzymes for cosmetics application
Bokrová, Jitka ; Čarnecká, Martina (referee) ; Márová, Ivana (advisor)
Presented diploma thesis is focused on testing of an appropriate form of encapsulated enzymes intended for application in cosmetic and pharmaceutical industry. For encapsulation, proteolytic enzymes bromelain, papain and collagenase were used. These enzymes were encapsulated into alginate and chitosan microparticles prepared by an encapsulator and packed into liposomes. Encapsulation effectiveness was evaluated by analysis of total proteins. Particles stability was evaluated in model and real conditions by photometrical analysis of released proteins. Proteolytic activity of released enzymes in model and real conditions were observed too. Alginate and chitosan microparticles prepared by the encapsulator were found as an appropriate form of encapsulated enzymes designed to wound healing. Encapsulation effectiveness of these particles and stability in model conditions were good in comparison with liposomes. Hydrogel and water-oil emulsion were used for analysis of particles stability at real conditions. Hydrogel was found as a good option for preservation of particles as well as proteolytic enzyme activity. Emulsion made particles less stable and proteolytic activity of enzymes decreased rapidly. Encapsulation enables long-term stabilization of biologically active compounds as well as possibility of targeted transport and controlled releasing. Presented diploma thesis suggests possibilities of application encapsulated enzymes in designing more effective formulations for wound healing.
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Preparation of organic fibers with the addition of algae extracts
Tuhrinská, Terézia ; Skoumalová, Petra (referee) ; Němcová, Andrea (advisor)
The presented bachelor thesis is focused on the preparation and characterization of organic fibers with an admixture of pre-prepared extracts of microalgae and cyanobacteria containing valuable active compounds. The theoretical part describes selected metabolites of microalgae and cyanobacteria, methods of their analysis, the issue of encapsulation and incorporation of active substances into nanofibers and presents some methods of their preparation. In the experimental part of this thesis, selected strains from the CCALA collection were first cultured. Subsequently, aqueous, ethanol and hexane extracts of biomass from cultured and commercial microalgae and cyanobacteria were prepared. The prepared extracts were spectrophotometrically evaluated in terms of the concentration of chlorophylls, carotenoids, antioxidants, polyphenols, and total proteins present. In addition, 96 % ethanol extracts were subjected to more accurate analysis of chlorophylls and carotenoids by HPLC. The highest antioxidant activity was observed for aqueous extracts. Gelatin nanofibers containing selected extracts were formed by electrospinning method. The formed fibers were examined afterwards to identify a mass fraction of incorporated microalgal and cyanobacterial substances. The fibers were further tested for antioxidant activity with the TEAC assay. The most pronounced antioxidant effect was detected for the fiber with incorporated aqueous extract of the cyanobacterium Arthrospira maxima. Finally, the fibers were tested for their safety in contact with human cells. The amount of fibers used did not show any cytotoxic effect on human keratinocytes and the tested materials can thus be considered safe for application in cosmetics.
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Selected alcohol extracts of Sambucus nigra for use in cosmetology
Balonková, Daniela ; Doležalová Weissmannová, Helena (referee) ; Zemanová, Jana (advisor)
This thesis focuses on the preparation of selected alcohol extracts of elderberry and their use in cosmetology. In the theoretical part are described the botanical properties of the elderberry (Sambucus nigra). There are introduced and characterized bioactive compounds and toxic compounds contained in all vegetative parts of the plant. Attention is mostly focused on compounds with antioxidant properties. The theoretical part also contains a description of basic analytical methods, which are used in the practical part to characterize compounds contained in elder flowers. The practical part focuses on the optimization of the preparation of alcohol extracts with the aim to prepare the extract with the greatest possible content of polyphenols with respect to economy and production costs. Factors such as selection of the appropriate solvent, solvent to plant material ratio, solvent temperature, and extraction time are examined. Based on the total polyphenol concentration measured spectrophotometrically by the Folin-Ciocalteu method, the most suitable solvent is 60% ethanol, the ratio of plant material to solvent is 1:8, the laboratory temperature of solvent and the extraction time of 24 hours.Under these conditions, an ethanol extract is prepared, analyzed and determined selected properties, such as refractive index and density. Rutin, chlorogenic, caffeic and ferulic acids are identified by HPLC with a DAD detector. The pH value of alcohol extract is 5.743 ± 0.006, the refractive index is 1.365 ± 0.02 at 22.7 ° C and the density is 0.9288 gcm-3. By liquid chromatography the concentration of chlorogenic acids is quantified as 0.104 gl-1, caffeic acid as 0.086 gl-1 and ferulic acid as 0.060 gl-1. The extract is incorporated into cosmetic products, that are used in balneology. Prepared products are toilet soap and bath salt.
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Restoration of the chateau and manor house in Vážany nad Litavou
Machová, Kamila ; Marek, Jiří (referee) ; Šindlar, Jiljí (advisor) ; Struhala, Karel (advisor)
This work aims to restore the castle and the manor house in Vážany nad Litavou. This building is located in the centre of the southern part of the village of Vážany nad Litavou, on the main street. The building is situated roughly in the middle of a series of estates on the west side of this street. The building is a rugged corner building. The building is not a cultural monument, but as part of this work, we try to treat the building as if it were a cultural monument. The history of the building is not well documented and the first written references date back to 1643. In the 16th century, the Jesuits built not only an economic building in Vážany nad Litavou but also a chateau in the corner position, which is partly palatial and partly ground floor. In the 19th century, the castle is transformed into a classical castle. After 1945, the entire facility was confiscated and forfeited to the state. Five years later, the newly established single agricultural cooperative was put into administration. Cooperative offices were created in the building and two housing units were created on the floor, which later replaced only storage space. Today, the castle building serves the needs of various private institutions and has remained as it was acquired during the early 19th century. My prosal aims to restore the structure to serve the public again. Interference with the exterior appearance from the aisle section will be demonstrated by replacing and increasing the rafters in the right and left wings of the building, replacing roofing, adding roof windows and replacing existing windows with six-table windows. The building gets a new facade. The appearance of the building from the side of the yard will be complemented by a rectangular extension of steel with a glass facade and decorated art stained glass. This addition will include a new staircase.
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Antimicrobial substances from plants and their application in acne preparations
Alexová, Adéla ; Byrtusová, Dana (referee) ; Němcová, Andrea (advisor)
The aim of the bachelor thesis is a determination of selected active substances of various plants and their possible application in cosmetic products for acne. In the theoretical part, an inventory of active substances including their effects in cosmetic products has been developed. Furthermore, the work is focused on characteristics of certain types of plants, mechanisms of antimicrobial effects and the emergence and treatment of acne. In the experimental part of this work the contents of biologically active substances, antioxidant and antimicrobial activity in extracts from selected plant species are characterized, namely from flowers of Levandula angustifolia, Matricaria chamomilla, Calendula officinalis, Syzygium aromaticum, leaves of Salvia officinalis and leaves of Rosmarinus officinalis. The plant extracts are also tested in different mixtures. Plant extracts and mixtures are incorporated into a recipe of acne cosmetic products. Their inhibitory effect against Propionibacterium acnes which causes acne and against representatives G+, G- and yeast Micrococcus luteus, Escherichia coli and Candida glabrata is observed. From the results it is clear that the best products against acne are micellar water and possibly skin lotion. The biggest amount of active substances is in cosmetic products which contain Matricaria, Syzygium and Rosmarinus. Overall effect of active substances is lower in cosmetic products than in 100% extracts.
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