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Streptophytic Algae with a Focus on the Class Zygnematophyceae
Špička, Jan ; Soldán, Zdeněk (advisor) ; Ráček, Jan (referee)
This bachelor's thesis deals with streptophyte algae, with special attention given to the class Zygnematophyceae. The thesis is written as a literature review, aiming to summarize existing knowledge about streptophyte algae, particularly focusing on the class Zygnematophyceae. Additionally, the thesis elucidates the phylogenetic relationships within this group, which historically have been challenging. The thesis primarily focuses on the description, ecology, and taxonomy of streptophyte lineages, emphasizing their significance in the study of terrestrialization, or the colonization of land. A significant part of this thesis is therefore to highlight the ecological and evolutionary role of streptophyte algae, especially in the context of their adaptation to terrestrial environments. An additional aim of the thesis is to address the issues associated with the group of algae, given their inconsistent definition. The thesis points out that algae do not form a monophyletic group, as they encompass various taxonomic lineages. This thesis can serve as a foundation for further research in the field of algology, thereby contributing to a better understanding and orientation in the process of terrestrialization. It also indicates the need for more phylogenetic studies to enhance understanding of the...
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Použití alternativních aditivních látek v odchovu telat
VALTOŠOVÁ, Gabriela
The diploma thesis consists of a literature research, which is then used for compari-son with the results of the experiment and a practical part investigating the effect of various prebiotics and probiotic preparations on the health and growth of calves in the period of colostrum and milk nutrition.
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Isolation of active compounds from algae and their application in cosmetic products
Nováková, Laura ; Byrtusová, Dana (referee) ; Skoumalová, Petra (advisor)
The bachelor thesis is dedicated to the study of active substances and antioxidant effects of cultivated microalgae. We compared the results with a commercial alga that is freely available. In the theoretical part we focused on description of algal metabolites in cosmetics and a brief description of the methods used in their testing. In the experimental part there were analyzed three types of extracts (aqueous, alcohol and oil) from 4 species of algae. It is mainly the content of polyphenols, flavonoids and antioxidant activity that is determined. According to the results, the highest content of active substances was found in oil extracts. All extracts were encapsulated into liposomes. The average size of the nanoparticles was in range of 200 nm to 300 nm. The particles were stable enough also for long-term stability. The largest decrease was observed in the oil extract of the cultivated algae Chlorella vulgaris. The cytotoxicity of oil extracts was realized by MTT test on human cell cultures HaCaT. Finally, we concluded that the prepared liposomal particles could be used for anti-aging cosmetics.
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Encapsulation of active components and their applications in food industry
Malčíková, Hana ; Němcová, Andrea (referee) ; Skoumalová, Petra (advisor)
The Diploma thesis is focused on encapsulation of bioactive compounds which are contained in selected superfoods for the purpose of application to childrens nutrition supplements. In view of the nature of selected samples, which are seaweed, walnuts, hemp seed and flax seed, the emphasis is put on the content of omega-3 and omega-6 acids. The theoretical part introduces topic of children food, further it describes the nature of selected superfoods and last but not least it contains screening of available childrens food supplements containing omega-3 fatty acids on the market. In the experimental part, an optimization of the appropriate type of extract was made. A 24 hour aqueous macerate and 20% ethanol macerate were selected for next analyzes. Samples were characterized by content of polyphenols, flavonoids and the antioxidant activity and they were used to forming of extracts were encapsulated into liposomes for which their encapsulation efficiency, stability and long-term stability have been tested. Hexan extracts were also prepared for the same reasons. It was found that liposomes of selected superfoods should not be stored for 12 weeks. The samples were used analyzed for the carbohydrate content by the duboise method, a protein content by the Kjeldahl method and the determination of the fatty acid profile by gas chromatography. Furthermore, the content of carotenoids and chlorophylls in algae samples was determined by spectrophotometry and this analysis was verified by high-performance liquid chromatography. The technique of high-performance liquid chromatography was also used to verified a content of polyphenols in the algae samples. Finally, a sensory analysis of prepared alginate supplements containing algae and cold-pressed oils was performer in combinations to achieve a higher omega-3 and omega-6 fatty acid content. The F01-labeled sample containing wakame algae was evaluated as the best one.
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Production of lipids and lipid-soluble compounds by some yeasts and algae
Šimanský, Samuel ; Rapta, Marek (referee) ; Márová, Ivana (advisor)
The submitted bachelor thesis focuses on production of lipids and lipid-soluble compounds by selected strains of carotenogenic yeasts, autotrophic algae and cyanobacteria. Different forms of stress were applied on selected strains and its influence was subsequently compared. The theoretical part deals with the processing of knowledge about selected strains of yeast, algae and cyanobacteria. There are also mentioned the selected metabolites (lipids, carotenoids, ergosterol, ubiquinone and chlorophyll) and the possibilities of their determination. The experimental part deals primarily with the influence of nutritional stress on the production of selected metabolites and the yeast biomass, namely their growth properties on waste fat. Furthermore, there was monitored the effect of elevated and reduced temperature on the cultivation of yeasts, algae and cyanobacteria. Osmotic stress experiments were also carried out by the addition of NaCl. As the most suitable strain for decomposition of the waste fat was selected Sporidiobolus pararoseus, particularly because of its high content of carotenoids, ergosterols and ubiquinone in biomass. Changes in temperature, or increased osmotic pressure had in the most cases a negative effect on biomass production.
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Study of the ETICS surface resistance to biotic attack
Těžká, Adéla ; Žák,, Antonín (referee) ; Žižková, Nikol (advisor)
This diploma thesis deals with the study of resistance of ETICS surface treatments to biotic attack. The theoretical part elaborates a research focused on biotic attack of facades of buildings insulated by contact insulation systems ETICS. Based on the information obtained during the elaboration of the bachelor thesis and further study, the optimization methodology of ETICS surface treatment resistance to biotic attack proposed in 2018 is elaborated. In the practical part, the proposed methodology is verified, evaluated and recommendations concerning the suitability of using the proposed methodology in construction practice are implemented.
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Optimization of extraction of pigments from yeast and algae cells
Šimanský, Samuel ; Mikulíková, Renata (referee) ; Márová, Ivana (advisor)
The presented diploma thesis deals with the extraction and storage stability of lipophilic pigments produced by selected strains of yeasts and algae. In this thesis, there was studied the influence of the selected solvents on the efficiency of extraction, as well as the effect of ambient temperature on the stability of the pigments during storage. The work is divided into two main parts, theoretical and practical part. In the theoretical part knowledge about algae, yeasts and their cultivation parameters is summarized. Furthermore, some information regarding the selected lipid metabolites, their properties and possibilities of application in various branches of industry is mentioned. The practical part deals with the preparation of extracts and stability tests. Extracts were prepared from selected biomass samples in solvents suitable for applications in food industry or cosmetics (ethanol and hexane). Subsequently, the long-term stability tests lasting 4 months and short-term stability tests lasting a total of 28 days were performed on these extracts. The pigments were determined by HPLC and spectrophotometrically, the fatty acid content was determined by GC. For the extraction of pigments from biomass, in the most cases ethanol appears to be the optimal solvent. However, for lipid extraction from biomass, hexane appears to be the optimal solvent for a significant number of samples. In most samples, storage in the freezer showed the most favourable effect on pigment stability, but some samples showed comparable stability even when stored in the refrigerator.
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Determination of phosphates in waters and characterization of their negtive effects on water ecosystem
Novotný, Radek ; Komendová, Renata (referee) ; Sommer, Lumír (advisor)
This bachelor thesis is focused on the negative effects of phosphates in natural waters and their methods of determination. The first part is characterized by a negative impact on natural water and there are characterized the problems associated with eutrophication, development of cyanobacteria and algae and the resulting potential health risks. The second section describes the methods by which phosphates can be determined. The work highlights the particular spectrophotometric methods such as molybdophosphoric acid, vanadomolybdophosphoric acid, wolframophosphoric acid, phosphomolybdenum blue and methods with some basic dyes such as rhodamine B, rhodamine 6G, malachite green and crystal violet. There are also mentioned methods, which are used less frequently, but they are relatively sensitive, such as AAS, AES, LC-ICP-MS and next such as chromatographic, electrochemical and enzymatic methods. Finally, work mentions the possibility of using automatic techniques such as FIA or CFA, which are suitable for routine and frequent determination, often using the spectrophotometric methods, but not only these.
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