|
| |
|
|
Birds in teaching at secondary schools with new knowledge in the field
Weiser, Jiří ; Řezníček, Jan (advisor) ; Kopřivová, Kateřina (referee)
In the introduction of theoretical part of diploma thesis is tabularly summed up the systém of birds classification. Other parts are focused not only on general issues and commonly used themes in school textbooks, popular literature and educational media, but also to highlight specific features, characteristic only for the birds. These themes are historical and evolutional questions, cover morphology, the effect of the senses, body structury and function of organs. In detail is analyzed the issue of body composition and skeletal system of birds. In the final sections are mentioned specialities in the behavior and communication between birds and their habits. The practical part includes a SWOT analysis of available textbooks for the four-year gymnasium with regard to the chapters and information about the animal class of birds. The data analyzed in the textbook can only be considered as basic with need to extend and complement by the teacher. Attached to the diploma thesis are two presentations in Powerpoint, containing basic information and specific features, characteristic only for birds. The presentations are created worksheets. For reference classes was developed as a lab practical examination of selected thematic blocks, memorized theoretical interpretation. Students' knowledge of the...
|
|
|
Streptomycetes surface growth and differentiation on inert microbeads- morphology and proteome study
Tesařová, Eva ; Weiser, Jaroslav (advisor) ; Lichá, Irena (referee)
Streptomyces, filamentous Gram-positive bacteria are producers of more than 70% of antibiotics used in human therapy and agriculture. They are remarkable because of their complex life cycle (morphological differentiation) which leads to a formation of dormant spores able to survive unfavorable living conditions and allowing long-term survival of the organism. Soil represents their mostly natural living environment. In laboratory conditions they are cultivated in liquid media or on agar. We have developed in our laboratory two phase cultivation system which allows quantitative and reproducible preparation of samples for proteomic, transcriptomic and metabolomic analyses of Streptomycetes differentiation. The system is composed of inert micro- beads submerged in liquid medium. We used two types of micro-beads in our studies, glass and zirconia/silica beads. We followed the surface growth and differentiation of Streptomycetes on both types of beads using optical and electron microscopy (SEM) techniques. We observed major growth and higher antibiotic production on glass beads. Another difference we observed was in size and shape of colonies. In further research, using comparative proteomics, we attempted to identify proteins which might be responsible for recognition and adhesion of Streptomycetes to...
|
|
|
Bacterial biofilm formation
Sochorová, Zuzana ; Weiser, Jaroslav (advisor) ; Zikánová, Blanka (referee)
Biofilms are formed by microorganisms living together in a hydrated extracellular matrix. Formation of such clusters of bacteria brings many benefits. The increased resistance to antibiotics is the main one. Creating a biofilm is analogous to the development of multicellular organisms. Biofilm cells communicate with each other with signaling molecules. Signaling molecules make the biofilm more compact structure. Extracellular matrix, in which bacteria live, maintains biofilm structure, affects cell adhesion and protects cells against environmental influences. Bacteria also interact with the environment through the extracellular matrix. The matrix is composed of various biopolymers and proteins. Biofilms are a common cause of infections associated with implants. There are several ways to prevent bacteria in biofilm to antibiotics. These include a slow diffusion of substances, a slow growth or an adaptation to stress. The formation of persistent cells that are tolerant to the antibiotics is the cause of their survival as well as a new population may arise from them. The surface proteins are important elements for the formation of biofilms, they facilitate adhesion and subsequent establishment of biofilm. A protein Bap was the first identified protein. Later its homologues were found, such as BapA,...
|
|
|
Analysis of intracellular pathogen Francisella tularensis expression upon cultivation under stressfull conditions and during the interaction with a host cell
Lenčo, Juraj ; Stulík, Jiří (advisor) ; Rychlík, Ivan (referee) ; Weiser, Jaroslav (referee)
Univerzita Karlova v Praze Lékařská fakulta v Hradci Králové Analýza exprese intracelulárního patogena Francisella tularensis kultivovaného za stresových podmínek a v průběhu interakce s hostitelskou buňkou Autoreferát dizertační práce Mgr. Juraj Lenčo Hradec Králové 2007 2 Dizertační práce byla vypracována v rámci doktorského studijního programu lékařská biologie na Ústavu lékařské biologie a genetiky Lékařské fakulty v Hradci Králové, Univerzity Karlovy v Praze a Ústavu molekulární patologie Fakulty vojenského zdravotnictví Hradec Králové, Univerzita obrany Brno. Uchazeč: Mgr. Juraj Lenčo Ústav molekulární patologie, Fakulta vojenského zdravotnictví Hradec Králové, Univerzita obrany Brno. Školitel: Doc. MUDr. Jiří Stulík, CSc. Ústav molekulární patologie, Fakulta vojenského zdravotnictví Hradec Králové, Univerzita obrany Brno Školitel konzultant: Prof. MUDr. RNDr. Miroslav Červinka, CSc. Ústav lékařské biologie a genetiky, Lékařská fakulta v Hradci Králové, Univerzita Karlova v Praze Oponenti: RNDr. Ivan Rychlík, Ph.D. Oddělení bakteriologie Výzkumný ústav veterinárního lékařství Brno RNDr. Jaroslav Weiser, CSc. Mikrobiologický ústav Akademie věd České republiky Stanovisko k dizertační práci vypracovala oborová rada oboru lékařská biologie akreditovaného na Ústavu lékařské biologie a genetiky Lékařské...
|
|
| |
|
|
Molecular Phylogeny, Biology and Classification of the Microsporidia (Microsporidia,Fungi) According to the Model Groups from Caddis Flies (Insecta, Trichoptera) and Butterflies (Insecta, Lepidoptera).
Hyliš, Miroslav ; Vávra, Jiří (advisor) ; Koudela, Břetislav (referee) ; Weiser, Jaroslav (referee)
RESULTS: Theresultsof thePh.D.thesiscanbesummarizedinto4 thematicareas: l ) screeningand experimentaldataconcemingthe prevalence,genusand speciesdiversíty. hostandtissuespeciťrcityand of ecological. pathofyziologicalcharactersof infectionsand of theirtransmission. 2) classificationandtaxonomystudies 3) resultsofsequencingfollowedby phylogenyanalysis 4) resultsoťcomparativestudybasedon summarizeddata Ad r) T\."enty-fourmicrosporidiafrom Trichopteraand Lepidopteraas well as 5 microsporidiafrom potentialintermediatehosts (Crustacea,Amphipoda) were isolatedand screenedusing light andelectronmicroscopyandmolecularbiologymethods.The screenedorganismsrepresented thefollolving generain theconventionalclassificationof Microsporidia (Canrringand Vávra, 2000)ů,osema(7). Endoreticulatus (3), C1lstosporogenes(2). Thelohania (4), Gurleya (1), Cougourdella(l), Pyrotheca(l), Episeptum(5), Toxoglugea(1),Larssoniella (2),unresolved isolates(2)(thenumberofspeciesexaminedin respectivegenerais givenin parentheses). The publications(l-3) and the abstract(8) reportthe ecologicaland experimentaldataon the prevalence,hostand tissue specificity and transmission of l0 examinedmicrosporidia.The :.';!:rtion(.1)and the abstract (7) describe ecological and experimentaldata on several 'f microsporidiaof theglpsy moth,Lymantria dispar...
|
|
| |
|
| |
|
|
Structure-function relationships and use of RTX proteins of Gram-negative bacteria
Sadílková, Lenka ; Šebo, Peter (advisor) ; Stulík, Jiří (referee) ; Weiser, Jaroslav (referee)
RTX (Repeat in ToXin) superfamily consists of many proteins divided into several groups according to their different functions and characteristics: toxins, metalloproteases, lipases, proteins of the S-layer, bacteriocins and proteins with unknown function. However, all of them can be characterized by the following features: i) they contain tandemly repeated (6-50) nonapeptide glycine-rich calcium-binding consensus sequences GGXGXDX[L/I/V/W/Y/F]X (where X is any amino acid residue) in the C-terminal part of the protein. The presence of these repeats is a sine qua non condition for RTX protein family membership; ii) secretion from the cell occurs without a periplasmic intermediate by a mechanism which involves recognition of a signal sequence at the C-terminus of the protein by membrane-associated proteins that export the toxin across a channel spanning the entire bacterial envelope directly to the outside of the cell (Type I Secretion System); iii) the genes for protein synthesis, activation and secretion are mostly grouped together on the chromosome and form rtx operons. RTX toxins are the largest protein group of the RTX family. To this group belong mostly the proteins with molecular weight ranging from 100 to 200 kDa, with posttranslational fatty acid acylation mediated by a specific activating...
|
guest ::
RSS feed.