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Thermodynamic Stability of Thaumasite
Pospíšilová, Klára ; Dvořák, Karel (referee) ; Fridrichová, Marcela (advisor)
This work deals with the resolution of the identification characteristics of the thaumasit, which falls into the same group of AFt phases as the mineral ettringit. Both of these stages have the same structural arrangement and the morphology. This work’s specific goal is the synthetic preparation of thaumasit in a variety of conditions and its evaluation.
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Motivation and Stabilization of Key Employees in Ceska zbrojovka a.s.
Pospíšilová, Klára ; Chovanec, Josef (referee) ; Rašticová, Martina (advisor)
This master thesis focuses on the problem of motivation, stimulation and stabilization of the key employees in the company Česká zbrojovka a.s. The main aim of this thesis is to identify insufficiencies in the current system of employee motivation and to suggest potential changes leading to its improvement and assessment from the economic impact of the proposed improvements to the company. The theoretical part of the thesis contains the fundamental knowledge and base of the human resources management and also discusses various theories of work motivation. Reviewed problems of demotivation and unwanted employee turnover follow. The practical part of the thesis shows a qualitative analysis of the current system of the staff motivation in Česká zbrojovka a.s. There is also linked and combined theoretical knowledge with the corporate practice. The final part of the thesis is based on facts found from the survey which identifies imperfections in the existing system and formulates potential recommendations for improving employee motivation and stabilization in the analysed company in order to improve work performance, enhance job satisfaction and loyalty of employees to Česká zbrojovka a.s.
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Development of the school in the Louňovice pod Blaníkem in historical and regional context
Pospíšilová, Klára ; Uhlířová, Jana (advisor) ; Stará, Jana (referee)
The thesis "Development of the School in Louňovice pod Blaníkem in Historical-Regional Context" aims to map the emergence and evolution of the school in Louňovice pod Blaníkem against the backdrop of the region, examining the development and characteristics of the municipality within its own historical context. The political and educational context in relation to the educational laws of the studied period is also considered. The methods employed in this thesis include source analysis, inductive and deductive methods, and the project method. The historical section describes the regional, historical, and economic development of the municipality, as well as the establishment and evolution of the school throughout history.The practical part focuses on a demonstration of project-based teaching in the third year. The aim is to bring students closer to the history of the school and the township, allowing them to process acquired information through experiential learning. The outcome of the thesis is a summary of the historical development of the township and school in Louňovice pod Blaníkem, along with the implementation of the project "School: Past and Present." KEYWORDS Historical development, primary education, chronicles, memorial books, school documentation, Louňovice pod Blaníkem, regional history.
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Interaction partners of protein eIF4E2 in human cells
Pospíšilová, Klára ; Pospíšek, Martin (advisor) ; Hálová, Martina (referee)
Protein eIF4E2 belongs to the family of eukaryotic translation initiation factors 4E, but it does not participate in translation initiation under normal circumstances. Its main role lies in translational repression of specific mRNAs. Nevertheless eIF4E2 takes part in translation initiation as a subunit of a specific translation initiation complex in hypoxic conditions. The exact mechanism in which eIF4E2 takes part in either of these processes is not known. One way to study the role of eIF4E2 in the cell is to find out what other proteins does eIF4E2 interact with. The goal of this work was to seek out potential eIF4E2-interacting partners in the HEK293 cell line using immunoprecipitation followed by mass spek- trometry. Apart from finding individual proteins the goal was to identify eIF4E2-containig protein com- plexes in HEK293 cells. A second line of work was preparation of a system for screening inhibitors of the interaction between eIF4E2 and eIF4G3. The main result is finding potential new eIF4E2-intera- cting partners in human cells.
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Study of some markers of human leukemia
Pospíšilová, Klára ; Jílek, Petr (advisor) ; Skálová, Lenka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study Field: zbioanalytical chemistry Candidate: Klára Pospíšilová Thesis Supervisor: PharmDr. P. Jílek, PharmDr. Consultant: RNDr. P. Řezáčová, MD., Ph.D., Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic Thesis Title: Study of some markers of human leukemia Abstract: Immunophenotyping of leukemia cells is an important part of leukemia diagnosis. Immunophenotype is determined by flow cytometry using monoclonal antibodies against antigens expressed by these cells. Antigen CD44 is one of many CD markers used in immunophenotyping of leukemias. Protein crystallography of a complex between CD44 antigen and its ligand, hyaluronate, bring more detailed information about the structure of the binding site, which may help develop strategies for influencing the binding and thus for potential therapeutic intervention.
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Use of specific inhibitors of carbonic anhydrase IX for fluorecent imaging of cancer cells
Pospíšilová, Klára
Human carbonic anhydrases are metalloenzymes that are involved in many physiological processes in the body, but also play an important role in the pathogenesis of numerous diseases. Under regular conditions, expression of carbonic anhydrase IX (CAIX) is very limited, unlike that of other 14 human carbonic anhydrase isozymes. But in hypoxic tumors this enzyme is highly overexpressed on the cell surface. For this reason, this enzyme represents a good target for therapy and diagnosis of tumors and thus various anti-CAIX monoclonal antibodies and specific inhibitors are being developed. In this work we investigated the possibility to use fluorescent polymer conjugate carrying a CAIX specific inhibitor for florescent labeling of tumor cells. Specific binding of polymer conjugate to different cell lines was investigated by flow cytometry and confocal microscopy. Binding properties of the polymer conjugate was compared to CAIX specific monoclonal antibody M75 and its single-chain fragment scFv M75. Ability of the polymer conjugate to inhibit CAIX enzyme activity was also investigated. For these experiments, recombinant protein CAII was prepared and purified, which was also used for protein crystallization. Tests of inhibitory activities allowed to identify novel inhibitors CAIX with better inhibitory...
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Interaction partners of protein eIF4E2 in human cells
Pospíšilová, Klára ; Pospíšek, Martin (advisor) ; Hálová, Martina (referee)
Protein eIF4E2 belongs to the family of eukaryotic translation initiation factors 4E, but it does not participate in translation initiation under normal circumstances. Its main role lies in translational repression of specific mRNAs. Nevertheless eIF4E2 takes part in translation initiation as a subunit of a specific translation initiation complex in hypoxic conditions. The exact mechanism in which eIF4E2 takes part in either of these processes is not known. One way to study the role of eIF4E2 in the cell is to find out what other proteins does eIF4E2 interact with. The goal of this work was to seek out potential eIF4E2-interacting partners in the HEK293 cell line using immunoprecipitation followed by mass spek- trometry. Apart from finding individual proteins the goal was to identify eIF4E2-containig protein com- plexes in HEK293 cells. A second line of work was preparation of a system for screening inhibitors of the interaction between eIF4E2 and eIF4G3. The main result is finding potential new eIF4E2-intera- cting partners in human cells.
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