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Model of experimentally regulated expression of dipeptidyl peptidase IV in glioma cell lines
Němečková, Jana ; Šedo, Aleksi (advisor) ; Vaníčková, Zdislava (referee)
"Dipeptidyl peptidase IV Activity and/or Structure Homologues" (DASH) represent a newly defined group of multifunctional molecules, typically bearing dipeptidyl peptidase IV-like hydrolytic activity. Dipeptidyl peptidase IV (DPPIV, EC 3.4.14.5, identical to CD26) cleaves out X-Pro dipeptides from the N-terminus of peptides. Recent knowledge shows substantial role of DASH in cancer pathogenesis. Here we present (i) an overview of the issue of DASH molecules and their functional substrates in the neuroectodermal tumor and (ii) a preparation of stable transfected human glioblastoma cell lines with inducible gene expression of DPPIV. Vectors containing human DPPIV gene and its mutated form in the catalytic active site have been prepared to assess the importance of the enzymatic activity of the final product. This will enable us to study the biological role of DPPIV in genesis and progression of neuroectodermal tumors - cells growth, invasion and migration of transformed glial cells in vitro. Moreover, complex role of DPPIV will be studied using a model of homotopic application of transfected cells into the brain of immunodeficient mice. Prepared cell lines provide more consistent information about DPPIV from the point of view of its "autocrine" importance for the expressing cells, as well as its potential...
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Family Guidebook to The Museum of Decorative Arts in Prague: An Example of the Lifelong Learning Concept Implemented in a Museum
Němečková, Jana
The thesis deals with the possibilities o f supporting and enhancing the educational role o f museums in the lifelong learning process, particularly in the process o f family learning. The author defines the conditions o f the preparation o f a self-guided museum programme for families. The author presents an example o f a family guidebook to the exhibition hall o f glass and ceramics o f The Museum o f Decorative Arts in Prague (hereafter UPM). The family guidebook draws on the study o f primarily British and American sources dealing with museum education and related disciplines. The thesis also features an analysis o f a small visitor study o f family visitors o f the UPM and a description and analysis o f a museum programme during which the guidebook was tested. According to the author, a self-guided museum programme for families should draw on family audience segmentation and on specific needs o f this visitor group. Such a programme should motivate visitors to learn actively from the exhibits, to encourage cooperation and sharing o f knowledge and skills among individual family members. The programme should make the visitors feel that the museum is a plače not only to learn new things but also to spend enjoyable moments, and encourage them to repeat their visit.
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Family Guidebook to The Museum of Decorative Arts in Prague: An Example of the Lifelong Learning Concept Implemented in a Museum
Němečková, Jana ; Dvořáková, Michaela (advisor) ; Havlínová, Ivana (referee)
The thesis deals with the possibilities of supporting and enhancing the educational role of museums in the lifelong learning process, particularly in the process of family learning. The author defines the conditions of the preparation of a self-guided museum programme for families. The author presents an example of a family guidebook to the exhibition hall of glass and ceramics of The Museum of Decorative Arts in Prague (hereafter UPM). The family guidebook draws on the study of primarily British and American sources dealing with museum education and related disciplines. The thesis also features an analysis of a small visitor study of family visitors of the UPM and a description and analysis of a museum programme during which the guidebook was tested. According to the author, a self-guided museum programme for families should draw on family audience segmentation and on specific needs of this visitor group. Such a programme should motivate visitors to learn actively from the exhibits, to encourage cooperation and sharing of knowledge and skills among individual family members. The programme should make the visitors feel that the museum is a place not only to learn new things but also to spend enjoyable moments, and encourage them to repeat their visit.
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Goverment Information Systems
Vaculík, Lukáš ; Němečková, Jana (referee) ; Koch, Miloš (advisor)
The work focuses on review of the present state of informatization of state administration on the South Moravian Region. This work approach communication between citizens and state administration. In this work are introduced two project - CzechPOINT and Virtuos which are focused on informatization of state administration.
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