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The Influence on Production of Secondary Metabolits in Tissue Culture Hypericum perforatum (L.)
Machačková, Jana ; Martin, Jan (advisor) ; Tůmová, Lenka (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Farmacognosy Candidate Mgr. Jana Machačková Consultant PharmDr. Jan Martin, Ph.D. Title of Thesis The influence on production of secondary metabolits in tissue culture Hypericum perforatum (L.) Using of precursors is one of the biotechnological methods, where precursors are added to the culture medium. Callus and suspensions cultures of Hypericum perforatum L. were used. Fenylalanin and cinnamoyl acid in concentrations 20,0 g/l, 2,0 g/l and 0,2 g/l, resp. 25,0 g/l, 2,5 g/l and 0,25 g/l were used as precursors. The effect of precursors on the production of flavonoids was evaluated after 4, 24 and 168 hours. Cultures were cultivated on Murashige and Skoog medium with addition 2 mg/ml growthing hormon BAP. HPLC method for analysis was used. Generally, the highest increase of flavonoids was made by precursor fenylalanin in concentration B in callus and suspensions cultures. The top in production of quercitrin was detected in suspension cultures after application fenylalanin in concentration C after 168 hours cultivation (from 0,16 % to 0,22 %). Hyperosid was the most increased by fenylalanin in concentration C after 4 hours cultivation (from 0,24 % to 0,35 %). Cinnamoyl acid as precursor is not suitable for increase...
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A Floristic Study of Petřín
Macháčková, Jana ; Dostál, Petr (advisor) ; Skýbová, Jana (referee)
The first part of the thesis describes culture-historical and natural features of the gardens at Petřín. The reader learns about the protected area in the upper part of the gardens and crucial influences of human activity in this area in the past and present. The second part deals with the results of a dendrological survey carried out in the Kinský Garden and Nebozízek and floristic research of the nature monument Petřínská skalka in the period between spring 2004 and autumn 2005. The results of the mapping have been recorded in table files that relate to attached documentary maps. In the discussion, I compare the findings of the thesis to the results of inventoried materials and suggest possible causes of the differences obtaining between the two lists. In the didactic part, I suggest a way of using this area for educational purposes. It contains two tracks - one focused on timber species and the second on complex cognition of nature. Worksheets were for both the tracks. The text also includes documentary photographs of some monitored timber species and plants. Powered by TCPDF (www.tcpdf.org)
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In vitro cultures of medicinal of plants - VII
Machačková, Jana ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
In vitro cultures of medicinal of plants - VII Elicitation is one of the methods that can be used to increase production or accumulation of secondary metabolites in explants cultures. There were used callus and suspensions cultures of Silybum marianum (L.) Gaertn. Derivate N-phenylpyrazin-2-carboxamid was used as abiotic elicitor in concentrations c1=1.159x10-3 mol/l, c2=1.159x10-4 mol/l and c3=1.159x10- 5 mol/l. The effect of elicitors on the production (flavonolignans of silymarin complex and flavonoid of taxifolin) was evaluated after 6, 12, 24, 48, 72 and 168 hours. Cultures were cultivated on Murashige and Skoog medium with addition 10 mg/ml α-naphtylacetic acid (α- NAA). HPLC method for analysis was used. The maximal content of flavonolignans was reached after elicitor application in concentration c2 after 168 h in callus culture (0,08%). However the highest content of silydianin was detected after application of elicitor in concentration c1 (0.11 %) in suspensions culture. The maximal content of flavonoid taxifolin was noted in of nutritient medium of suspensions cultures.
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Rights to sign in Czech positive law
Macháčková, Jana ; Pítra, Vladimír (advisor) ; Růžička, Michal (referee)
and keywords The purpose of the thesis is to analyse the legal regulation and the system of registration of rights to signs, which are most commonly used on national as well as international markets, i.e. rights to a trademark, appellation of origin and a geographical indication. The aim of this thesis therefore includs definition of the registration system of the mentioned rights to sign with reference to their specifications, the registration ability conditions (taking into consideration the decision practise of the Industrial Property Office), as well as the different character of protection of their owners. Significant attention is also paid to the system of legal protection of the rights to sign with respect to the fact, that there exists the national, international and Communitary system of protection of these rights. The beginning of the thesis describes the structure of rights to sign, the corresponding specification of concept of intellectual and industrial property rights and it characterises the subject of industrial property rights with respect to the legal regulation, the conception of legal protection of industrial property rights and conditions of grant of protection. The introductory text deals with the general exposition to rights to sign and mainly to historical circumstances of...
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Perception and Interpretation of Fractions by Pupils in the 5th Grade of Primary School
Vašíčková, Jana ; Tichá, Marie (advisor) ; Macháčková, Jana (referee)
The essential part of this diploma thesis is a classification of works obtained from an investigation aimed on determination of conceptions of fractions by pupils in the fifth form of the primary school. The experiment consists of four tasks, which were given to children in four different classes. I have classified produced works according to bases of division, which I had established. Mainly concerned classifications are the thematic classifying, classifying in accordance with the "type of children's works" and classifying based on occurred interpretations of fractions. I was trying to find such criterions that would state in evidence of what conceptions and knowledge of fractions do the pupils in the fifth form have. According to gained results I think that I fulfilled this goal. When I was sorting the works of children, I found out that there is a huge amount of large-scaled works that can become basis for another research.
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Methods for overcoming differences in pace of students in mathematics lessons
Plívová, Anežka ; Havlíčková, Radka (advisor) ; Macháčková, Jana (referee)
- 4 - ABSTRACT This diploma thesis is focused on teacher's strategies in mathematics lessons with different work paces of pupils, specifically pupils with above-average pace. The theoretical part of the thesis summarizes and explains the basic concepts related to the identification of smart and gifted pupils. The next part is focused on models of talent, teaching pupils with above- average pace, as well as a transmissive and a constructivist approach to teaching. The used research method was observation and discussion in focus groups. The basis of the practical part of the diploma thesis is a set of collected information and its arrangement into the so- called typology of teacher strategies when working with a pupil who works at an above- average pace in mathematics lessons with the described advantages or risks of individual strategies. The main goal of this diploma thesis was to collect ways in which the teacher copes with the different paces of students in mathematics lessons, mainly focusing on pupils with above-average work rates. Thesis describes benefits as well as risks that could arise in the application of strategies for the described individual methods. KEYWORDS above-average pace, mathematics teaching, gifted pupil, bright pupil, teacher's strategy
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World of plane and world of space in pre-mathematical literacy
Lorencová, Karolina ; Kaslová, Michaela (advisor) ; Macháčková, Jana (referee)
The diploma thesis deals with the issue of distinguishing and denominating of geometric models by children aged five to six years. The aim of the work is to find out to what extent children are currently able to distinguish the world of the plane and the world of space and to what extent these two worlds merge for children. There are three hypotheses. H1: "Children are able to identify at least half of objects from a set of spatial bodies selected from a construction set.", hypothesis H2: "More than a half of children are confused naming planar and spatial objects." and hypothesis H3: "When identifying some of the objects familiar to children, they come up with their own names for them." To verify them, quantitative research was used, which also used audio recording to get data and to be able analyze the observed phenomena. The research confirmed all of those three these hypotheses. Two thousand two hundred data were analyzed. The work on a sample of hundred children shows what the level of object distinction and naming them is currently among preschool children in the Pilsen region. It opens up a number of other areas that need to be further addressed and lots of questions to be answered. These include mixing the world of space and the plane. KEYWORDS Space; plane; identification of 3D objects;...
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Activities for next expansion with mathematically gifted pupil at the primary school
Reslová, Eliška ; Kloboučková, Jaroslava (advisor) ; Macháčková, Jana (referee)
This diploma thesis is focused on issues connected to possibilities of developement of mathematically talented pupils from the first grade. In the theoretical part there basic terms related to the education of talented pupils in th Czech Republic are summarised and explained. In next part motivation, processes of motivatiom and the most frequent usage of Math competitions as a part of the education in the Czech Republic are presented. The base of the practical part of this diploma thesis is a set of non standardized tasks and problems, whose target is to activate the cognitive developement of talented pupils. Part of the practical part of this thesis is a protocol about an experiment and analysis of the particular tasks and activities for talented pupils. The main aim of this thesis is to emphasise the importance of the developement of talented pupils and compile/form enriching tasks for talented pupils during Maths lessons. KEYWORDS Talent, talent models, motivation, cognitive process, non standardized tasks, aktivity.
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Academic development of students with learning disability at the Faculty of Economics and Faculty of Fisheries and Protection of Waters
MACHÁČKOVÁ, Jana
My diploma work is divided into theoretical and practical part. In theoretical part I engage with particular defect of studying, their types and demonstration. I also deal with their diagnostic and amendment. In the connection to particular defects of studying I also describe the trouble of defect of behaviour, mostly ADHD, including the defect of studying of adults. I also focus on education of individual with the particular defect of studying at university. I describe the system of studying at university with the possibility of advantage for students with particular defect of stuyding. The end of my theoretical part I aim on University of South Bohemia itself, its history and operating of each faculty. In my practical part I deal with analyze of students´branch/programmes of daily studying in two faculties of University of South Bohemia - Faculty of Economy and the Faculty of Fishing. Each subjects which are taught in particular studying branch I analyze and I show the requirements which are needed to pass that subject. After this I try to deduce the charge on students which has been defined on the base of analyze of each students branch in different faculties. It will be confronted with the theoretical piece of knowledge, which relates to needs and rarity of the students with the particular defect of studying while being a student at university.
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