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Determination of bitrex using ultra-high performance liquid chromatography
Fuková, Kateřina ; Kubíčková, Anna (advisor) ; Křížek, Tomáš (referee)
In this work, a method for the determination of bitrex (denatonium benzoate) using ultra- high performance liquid chromatography was developed. A Kinetex C18 column (Phenomenex) with dimensions of 100×2.1 mm and a particle size of 1.7 μm was used for the determination. A mixture of acetonitrile with 0.1% formic acid (component A) and deionized water with 0.1% formic acid (component B) was used as the mobile phase. The best separation was achieved with the gradient program set: 0-2 min: 20 % → 70 % A (vol. %); 2- 3 min: 70 % A (vol. %); 3-3,5 min: 70 % → 20 % A (vol. %); 3,5-5 min: 20 % A (vol. %). The temperature of the column during the separation was 40 řC and flow rate was 0,3 ml/min. The developed method was partially validated. Repeatability was measured, the linearity of the method was verified for sample injection 1 μl and 5μl. For the same sample injection limit of detection and limit of quantification was determined. Using the developed method, seven real samples containing bitrex were measured. These samples differed in matrix. The samples were five different coolants, glass cleaner and a washing gel for black clothing. In coolant Carline Antifreeze G13 (producer OMA CZ) the concentration of bitrex was set at 23,4 ppm. In coolant CS Antifreeze G 13 (producer CLASSIC Oil) the concentration...
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Determination of antiepileptics in breast milk with chromatographic methods
Ernestová, Viktoria ; Hložek, Tomáš (advisor) ; Kubíčková, Anna (referee)
The present thesis is devoted to the development of a new method for the determination of a selected group of antiepileptic drugs (brivaracetam, eslicarbazepine, felbamate, gabapentin, lacosamide, perampanel, pregabalin, rufinamide) in human breast milk. The rationale for identifying antiepileptic drugs in this rather complex matrix is to assess and optimize the use of these drugs in breastfeeding mothers to avoid unnecessary and risky exposure of infants. The aforementioned drugs have various undesirable side effects that can have a negative impact not only on the mother but also on the breastfed infant. The protein precipitation method using acetonitrile/methanol mixture was used for sample preparation. A method based on liquid chromatography with tandem mass detection was developed and tested for the determination of the concentrations of these drugs. In the course of testing, different chromatographic columns with different stationary phases were tested to achieve optimal separation conditions and experimental set-ups for the full range of antiepileptic drugs tested.The whole methodology will be subsequently validated according to the general guidelines for clinical and toxicological method validation according to international standards and its use in routine laboratory practice will be intended.
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Performing in the band The Tap Tap and its value in life of person with physical disability
Nováková, Alžběta ; Němec, Zbyněk (advisor) ; Kubíčková, Anna (referee)
This bachelor thesis is focused on performing in a band The Tap Tap and its value in the life of a person with a physical disability. The thesis is divided into theoretical and practical parts. The first chapter of the theoretical part is devoted to the definition of physical disability. It describes basic terminology, and division of movement disorders and introduces social-psychological aspects of the physical disability. The second chapter of the theoretical part is devoted to the social inclusion of people with a physical disability. It describes models of approaches to people with disability, and the impact of physical disability on family, partnership, sexuality, and employment. Further, the chapter describes social care and services and organizations and associations for people with a physical disability. The third chapter is devoted to the introduction of the band The Tap Tap, its history, and the present. The practical part is realised as qualitative research. For data collection was chosen the method of semi-structured interviews. The interviews were conducted with members of the band The Tap Tap with a physical disability. The research aims to find out, what impact performing in this band has on the life of a person with a physical disability. The results of the research part were...
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Electrokinetic chromatography in nonaqueous and mixed solvents: Determination of critical micellar concentration
Hamráková, Katarína ; Křížek, Tomáš (advisor) ; Kubíčková, Anna (referee)
This thesis deals with the investigation of micelle formation following the addition of a surfactant to a background electrolyte in micellar electrokinetic chromatography and the transfer of these systems to nonaqueous and mixed solvents. The addition of organic solvents to the background electrolyte has a significant effect onmicelle formationandhence separation in electrokinetic chromatographyusinga micellar pseudo-stationaryphase. This effect has not yet been studied in depth. Information on the presence and properties of micelles in a given separation system is important for the understanding of separation processes but also for the development and optimization of analytical methods. In this work, the critical micellar concentration (CMC) of two surfactants, sodium dodecyl sulfate (SDS) and cetyltrimethylammoniumbromide (CTAB), was determinedby introducingthem into a mixed environment with non-aqueous solvents, acetonitrile and methanol. The critical micellar concentration was determined from the dependence of three variables on the surfactant concentration in the solution, namely, the effective mobility of the analytes and the viscosity and conductivity of the solution. Prior to the actual measurements of the effective mobility of the analytes, suitable analytes were selected,two which can...
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Study of retention and separation of peptides in HPLC
Boudová, Hana ; Kalíková, Květa (advisor) ; Kubíčková, Anna (referee)
This diploma thesis deals with the study of retention and separation of structurally different peptides and digest products of cytochrome c using reverse phase and mixed modes of liquid chromatography. As the model peptides, five unmodified dipeptides, nine dipeptides containing a protective benzoyl group on the N-terminal amino acid and eight therapeutic peptides containing five to ten amino acids were selected. The chromatographic behaviour of the model peptides was tested on three different columns: XBridge C18, XSelect CSH C18 and Atlantis Premier BEH C18 AX under different conditions. The influence of the pH of the aqueous part of the mobile phase, i.e. 2,1; 3,0; 4,7 and 6,8, and the amount of acetonitrile in the mobile phase on retention and separation of model peptides was tested. The effect of the concentration of ammonium acetate buffer, pH = 6,8 in the mobile phase on the retention and peak symmetry in the concentration range of 10-50 mmol L-1 was assessed. The chromatographic conditions for the baseline separation of 20 different model peptides were optimized. The conditions were as follows: XSelect CSH C18 column, mobile phase composed of acetonitrile/10mM ammonium acetate, pH = 6,8 (v/v) under gradient elution. The suitable chromatographic conditions for the analysis and identification...
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Enantioseparations of liquid crystalline materials
Vaňkátová, Petra ; Kubíčková, Anna (advisor) ; Čabala, Radomír (referee) ; Kohout, Michal (referee)
(EN) Liquid crystalline materials are an important domain of the material science. Chiral liquid crystals (CLCs) are researched for their unique chirality-derived properties. The development of new CLCs focuses both on appropriate molecular design to fit their intended purpose and also on ways of using widely available and less expensive sources of chirality to enable cost- effective production for a potential large-scale production. Methods capable of enantioseparation are needed mainly for chiral purity control of newly synthesized CLCs and for their potential use in scaling up for preparative purpose. Nowadays, enantioselective chromatography has evolved to be the method of choice for enantioseparations. This thesis aims to establish new approaches to chromatographic enantioseparations of CLCs by developing fast and robust enantioselective methods using two different ultra-high performance chromatographic techniques - sub/supercritical fluid chromatography (UHPSFC) and liquid chromatography (UHPLC). UHPSFC is introduced and successfully employed for the first time for this purpose. For some CLCs, high values of enantioselectivity and enantioresolution were achieved, opening the possibility of scaling up to semipreparative use. In UHPLC, two modes (reversed-phase and polar organic solvent mode)...
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