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Improvement of quality, function and transplantation outcomes of pancreatic islets using RNA interference
Kosinová, Lucie ; Kříž, Jan (advisor) ; Štechová, Kateřina (referee) ; Petrák, Ondřej (referee)
Transplantation of insulin producing tissue is the only therapeutic method so far allowing type 1 diabetic patients to achieve long-term independence of insulin administration. Transplantation of isolated pancreatic islets (PI) into liver represents a safer but less effective alternative to whole-organ transplantation. This is caused by a significant loss of transplanted islets within a short time after transplantation due to the instant blood-mediated inflammatory reaction (IBMIR). This reaction is triggered by molecules of tissue factor, abundantly expressed on the surface of PI cells. Tissue factor activates directly the coagulation pathway leading to platelet aggregation, complement activation, and infiltration of islet graft by leukocytes which results in a destruction of up to 60 % of transplanted tissue, and a formation of ischemic areas of downstream lying liver tissue. Tissue factor also stimulates angiogenesis which makes it necessary for revascularization of PI after transplantation. Inhibition of tissue factor gene in isolated PI using RNA interference provides an opportunity for short-term suppression of tissue factor expression, leading to protection of PI in the early post-transplantation period without hindering the connection of capillaries to the recipient vascular system later...
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Starting points for a new physics curriculum
Kolář, Petr ; Žák, Vojtěch (advisor) ; Ješková, Zuzana (referee) ; Kříž, Jan (referee)
This thesis presents starting and supporting points for a new secondary school physics curricu- lum and the methodology which was used. The research design was inspired mainly by the objectivist grounded theory. 29 in-depth semistructured interviews were conducted with elite Czech physicists and there were identified 79 ideas about a secondary school physics curricu- lum. After approximately two years, 56 ideas were used in the questionnaire survey where re- spondents expressed on a Likert scale their degree of agreement with the ideas. Respondents were natural scientists (non-physicists), experts on didactics of physics, secondary school phys- ics teachers and 26 of 29 physicists who were also participants of the interviews. In the ques- tionnaire survey, there were identified ideas on which all groups of respondents agreed and which are stable in time in the group of physicists.
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Effects of stable analogs of anorexigenic neuropeptides in models of metabolic syndrome
Mráziková, Lucia ; Maletínská, Lenka (advisor) ; Kříž, Jan (referee) ; Bardová, Kristina (referee)
Obesity is a worldwide health problem and an effective treatment is still scarce. Anorexigenic neuropeptides, such as prolactin-releasing peptide (PrRP), have a potential for the treatment of obesity and its complications, but in their natural form they have several limitations such as poor bioavailability, low stability and inability to cross the blood-brain barrier after peripheral administration. Recently we have designed lipidized analogs of PrRP. Lipidization makes this peptide more stable and able to act centrally after peripheral administration. The aim of this study was to investigate the chronic effect of PrRP palmitoylated at position 11 (palm11 -PrRP31) on obesity and obesity-related metabolic parameters and to clarify mechanisms of its action. We used three rodent models of obesity: Wistar Kyoto (WKY) rats with high-fat diet-induced obesity (DIO) having intact leptin and leptin receptor as well as rodents with disrupted leptin function: leptin deficient ob/ob mice and fa/fa rats with a disturbed leptin signaling. Consumption of a high-fat diet in DIO WKY rats increased their body weight, caused strong glucose intolerance and increased liver mRNA expression of enzymes of de novo lipogenesis. Palm11 -PrRP31 treatment significantly decreased cumulative food intake, body weight, plasma...
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Affecting the Status of Diabetes Mellitus Type 2 Through Diet and Lifestyle Changes
Prokešová, Petra ; Zlatohlávek, Lukáš (advisor) ; Kříž, Jan (referee)
The thesis deals with the disease diabetes mellitus type 2, especially compliance regime measures and education of patients with diabetes mellitus type 2 visiting diabetes clinics in Jeseník and in Prague. The work is devided into theoretical part and practical part. The theoretical part focuses on the characteristics of the diabetes mellitus, diagnosis and options of treatment. The practical part identifies and compares the degree of compliance with regime measures and education level in patients with diabetes mellitus type 2 in diabetes clinics in Jeseník and Prague. The thesis may outline the deficiencies in the education of patients with diabetes mellitus type 2 and their possible solution. Key words: Diabetes mellitus, education, prevention, diet, physical activity
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Intellectual property rights protection of computer programs, information systems and related services
Kalíšek, Jindřich ; Kříž, Jan (advisor) ; Frinta, Ondřej (referee) ; Pítra, Vladimír (referee)
Ochrana počítačových programů, informačních systémů Jindřich Kalíšek - Disertační práce a příbuzných služeb právem duševního vlastnictví Strana 1 z 3 Intellectual property rights protection of computer programs, information systems and related services Thesis Abstract 1) Dissertation thesis titled Intellectual property rights protection of computer programs, information systems and related services deals with the main legal approaches and their selected alternatives to the legal protection of computer programs, information systems and related software services. 2) The goal of the thesis is to analyse and provide the reader with consistent up-to-date information in particular on the two main forms of legal protection - copyright protection and industrial property protection of computer programs, to concisely describe their development, current status and key legal norms (international, EU and national) and that establish them. 3) The dissertation consists of an introduction, nine chapters and a conclusion. The thesis is divided into four units - introductory explanation, part of copyright law, part of industrial property (IP) law and final comparative part. 4) In the Introduction, the reader will get acquainted with the key concepts and issues elaborated in the thesis, as well as with the basic theoretical...
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Creation of geoinformatical system for mobile devices
Jakl, Michal ; Čábelka, Miroslav (advisor) ; Kříž, Jan (referee)
The aim of this thesis is to design and create a complex community geoinformatical system for mobile devices with Android OS. The first part presents currently available software tools, with an emphasis on user friendliness and minimal financial costs. The following sections deal with design of client-server system, selection and processing of data into databases and creating algorithms, ensuring the entire system. Emphasis is also placed on optimalization for mobile devices, bilingualism and user friendliness. The practical outcome and application of ideas from previous parts of this work is the creation of a mobile application DoPřírody!, which allows outdoor users to search the database of natural areas (parks, forests, cemeteries and others) and also is able to navigate the shortest route to the selected destination in real time. For registered users it is possible to rate, comment and edit existing areas and add new ones. The conclusion contains comparation with large map servers, the results of publishing application on the Google Play website and conclusions of testing in the community of users. Keywords: Android, network analysis, server, database, nature, mobile applications
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