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Fabrication of 3D diamond membranes for microfluidic systems
Varga, Marián ; Babchenko, Oleg ; Bauerová, Pavla ; Hruška, Karel ; Jurka, Vlastimil ; Kromka, Alexander ; Rezek, Bohuslav
Perfusion of cell medium, especially in microfluidic devices, can provide in-vivo-like conditions for cell cultures. The most recent demand on such systems is to include electronically active artificial cell support for in-situ monitoring. Diamond thin films exhibit advantageous combination of physical, mechanical, chemical, biocompatible and electronic properties for this purpose. In this work we explore two strategies for fabrication of self-standing three-dimensional nanocrystalline diamond membrane for implementation in microfluidic invivo like experiments: i) nucleation and chemical vapour deposition (CVD) growth of diamond on porous 3D carbon foam (with 80 pores per inch) and ii) selective diamond growth predefined by photolithographic processing using copper grid mask. The morphology and material quality of the fabricated membranes are characterized by scanning electron microscopy and Raman spectroscopy.
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Computational study of cellular assembly on hydrophobic/hydrophilic micro-patterns
Ukraintsev, Egor ; Brož, A. ; Kalbáčová, M.H. ; Kromka, Alexander ; Rezek, Bohuslav
We develop simple onedimensional stochastic model of cell behavior on chemically patterned surfaces that is based on three key parameters: speed of cell movement (motility) across substrate, probability of cell adhesion to substrate, and probability of cell division on substrate when adhered on substrate. Amount of adhered cells on hydrophobic and hydrophilic regions is calculated as function of time (number of cycles up to 2000). The model is correlated with in-vitro data obtained within 48 h in real time. We show that this simple stochastic model with the three parameters (where cell motility is the most important one) can describe with high accuracy the experimental data and thereby explain the observed preferential cell assembly on hydrophilic/hydrophobic micro-patterns (up to 200 um width).
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Synthesis, structure, and opto-electronic properties of organic dies on diamond
Rezek, Bohuslav ; Čermák, Jan ; Ukraintsev, Egor ; Hubík, Pavel ; Mareš, Jiří J. ; Ledinský, Martin ; Fejfar, Antonín ; Kočka, Jan ; Kromka, Alexander
We prepare a thin-film heterojunction of polypyrrole (Ppy) on hydrogen-terminated diamond by electro-polymerization from solution. We combine advanced scanning techniques (AFM, KFM, micro-Raman) to characterize microscopic structural, chemical, and opto-electronic properties of such system.
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