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The flow cytometric detection minorite populations CD19+ B cells specific for autoantigen in synovial tissue and peripheral blood of patients with rheumatoid arthritis
Růžičková, Š. ; Cimburek, Zdeněk ; Van Venrooij, W. J. ; Drijhout, J. W. ; Moravcová, T. ; Hůzlová, Z. ; Veselá, Z. ; Parkmanová, P. ; Kryštůfková, O. ; Vencovský, J.
The flow cytometric detection minorite populations CD19+ B cells specific for autoantigen in synovial tissue and peripheral blood of patients with rheumatoid arthritis
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Autofluorescence dřevokazné houby Fomes fomentarius
Žižka, Zdeněk ; Gabriel, Jiří
We observed the core of wood-rotting fungus Fomes fomentarius fruiting bodies in fluorescent microscopes. When excited with blue light, they featured thin-walled generative hyphae with septa and clamps, some of which emitted a strong yellow, yellow-green to yellow-red autofluorescence. In addition, we found yellow-red fluorescing thick-walled skeletal hyphae and strongly fluorescent thick-walled pith sets. On using green excitation, intensive red fluorescence was observed not in all these fruiting body components but also in the rest of the wood material
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Mikrofotografie rychle se pohybujících buněk
Žižka, Zdeněk ; Hostounský, Z.
Bacteria, algae and protozoans were observed and photographed under a standard laboratory microscope with a new LED diode illumination system. Our main work was focused on photographing flagellates and infusorians, which are much faster-moving than bacteria. The internal structures observed in flagellates including plastids, pyrenoids, vacuoles and granules, the infusorian structures were recorded, e.g. cytostomes, food and contractile vacuoles, granules, etc. A power output of LED diode is quite sufficient and makes it possible to photograph even highly motile microorganisms
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