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CRISPR/Cas9 Genome editing in Pyrrhocoris apterus
BERTOLUTTI, Maly
The CRISPR/Cas9 technology is a cutting edge method to genetically modify specific targets in the genome, which allows to create mutants in non-model organisms. The aim of this project was to genetically modify the Pyrrhocoris apterus genomic sequence of the cryptochrome2 gene using the CRISPR/Cas9 technology. Specifically, this project aimed to create a mutant with removed or modified C-terminal part of the CRY2 protein, which should later allow for studying the role of this sequence in CRY2 function.
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Zpráva z průzkumu brouků (Coleoptera) v Ptačím parku Josefovské louky 2017
Kopecký, Tomáš
Research of the Coleoptera, was provided in year 2017 in Bird Park Josefovské meadows (Královéhradecký district, Czech Republic). This global entomology research is the first, which was in this locations make. By the research has been found 217 species of Coleoptera, therefrom 6 species are protected in Czech republic: Carabus ullrichi ullrichi Germar, 1824 , Carabus scheidleri helleri Ganglbauer, 1892, Cicindela campestris campestris Linnaeus, 1758, Brachinus explodens Duftschmid, 1812, Meloe proscarabaeus Linné, 1758 and Oxythyrea funesta (PODA, 1761). Also has been documented five species from Red list of invertebrate of Czech Republic (Farkač, Král & Škorpík 2005): Cryptocephalus pusillus Fabricius, 1777 – (EN); Ampedus nigroflavus (Goeze, 1777) – (NT); Coraebus elatus (Fabricius, 1787) – (NT); Acupalpus luteatus (Duftschmid, 1812) – (VU) and Grypus brunnirostris (Fabricius, 1792) – (VU). In the database of AOPK is also reported species Hydrophilus piceus (Linné, 1758) wich is documented in Red list (Farkač, Král & Škorpík 2005) as critically endangered.
Plný text:  PDF
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