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Valuation of the biologically active substances by liquid chromatography IX.
Sommernitzová, Nora ; Kastner, Petr (referee) ; Pilařová, Pavla (advisor)
Valuation of the biologically active substances by high performance liquid chromatography II. (Validation of determination of metformin-hydrichloride by high performance liquid chromatography) Thesis Sommernitzová Nora Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control The method for determination of the metformin-hydrochloride by high performance liquid chromatography in pharmaceutical preparations Diaphage 500 and Diaphage 850 was optimalised. Using the principle of ion exchange chromatography, the metod was carried out on the μBondapack C18 (10μm) column. A mobile phase composed of sodium pentanesulfonate and sodium chloride at a flow rate 1,5 ml/min was used for the separation. pH was adjusted to 3,5 using phosphoric acid. The temperature was set at 25,0 řC. Detection was carried out at 218 nm. Following these conditions the metod was validated. The accuracy, precision, linearity and specifity were also verified. All tested parameters satisfied the criterions.
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Determination of Camphor Content in the Ointment Using Monolithic Column
Voříšková, Michaela ; Kastner, Petr (advisor) ; Mokrý, Milan (referee)
Determination of Camphor content in the ointment using monolithic column Thesis Mgr. Michaela Voříšková Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control The HPLC method for determination of camphor content in pharmaceutical preparation Unguentum camphoratum vaselinatum 10% using monolithic column was developed. The time of analysis was shorted on 1,3 min. The chromatographic column Chromolith Speed rod, RP-18e 50-4,6 mm and chromatographic precolumn Chromolith, RP-18e 10-4,6 mm with mobile phase water : methanol 35 : 65 were used. UV detector for the determination with the setting wave lenght 289 nm was used. The metod was validated for accuracy (recovery = 99,03 %), precision (RSD = 0,83 %), linearity (R = 0,9995) and for specificity. The metod was also tested in other pharmaceutical preparations containing camphor.
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HPLC evaluation of selected drugs II
Vyroubal, Petr ; Mokrý, Milan (advisor) ; Sochor, Jaroslav (referee)
HPLC evaluation of selected drugs II. THESIS Petr Vyroubal Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control In this thesis was validated method for simultaneous HPLC analysis of paracetamol and tramadol in combined tablet. As stationary phase the chromatographic column Discovery HS F5, 5 µm, 150×3 mm I.D. made by Supelco was used. Mobile phase was formed by mixture of acetonitrile : ammonium acetate solution 0.005 mol/l, acidified with acetic acid at pH 3.2 in the ratio 20:80 (v/v). A flow rate was 1.0 ml/min. Acetylsalicylic acid was used as an internal standard. The detection was performed at λ = 270 nm using an UV detector. The substances were eluted in following order: paracetamol, acetylsalicylic acid and tramadol. The method was validated for linearity (paracetamol R = 0.9996; tramadol R = 0.9998), precision (paracetamol RSD = 0.31 %; tramadol RSD = 0.70 %), accuracy (paracetamol recovery = 99.49 %; tramadol recovery = 98.90 %) and robustness.
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"The optimalization and validation of LC/MS method for the determination of selected diureticsin human blood serum"Optimization and validation of LC/MS method for quantitative determination of diuretics in human blood serum
Světlíková, Zuzana ; Nobilis, Milan (advisor) ; Kovaříková, Petra (referee)
A new method was developed and fully validated for the quantification of hydrochlorothoazide and chlorthalidone, both diuretic and anti-hypertensive agents, in human serum. This liquid chromatography-tandem mass spectrometry method (LC/MS/MS) is rapid, simple, accurate and selective. The analytes and the internal standard were extracted by liquid-liquid extraction (LLE) with ethylacetate-dichlormethane (80:20, v/v). The chromatographic separation was performed on a reversed-phase column C8 with a mobile phase acetonitrile-formic acid (0,2 %), the elution was gradient. The analytes were quantitated by linear ion trap mass spectrometry with an electrospray ionization interface (ESI). The diuretics were analysed in negative ion monitoring mode, the internal standard in positive ion monitoring mode. The assay exhibited a linear dynamic range of 0,5-200 ng/mL for hydrochlorothiazide and 0,5-500 ng/mL for chlorthalidone in human serum. Acceptable precision and accuracy were obtained for QC samples with concentration over the standard curve ranges. This developed and validated method has been used in the toxicological laboratory in the Institution of Clinical Biochemistry and Diagnosis in Hradec Králové.
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Land cover changes in District Nachod using remote sensing data
Červená, Lucie ; Kupková, Lucie (referee) ; Štych, Přemysl (advisor)
Land cover changes in District Nachod using remote sensing data Abstract The purpose of this project was to create a classification of the land cover of Náchod district for years 1979, 1991 and 2001 based on multi-spectral images gained from publicly available archive images database provided by Landsat satellites. Data used in this paper are described in details. The created classification system is based on CORINE Land Cover and adjusted to a measured area and data available. The method used for images classification was method of supervised classification in PCI Geomatics program and classification algorithm of Maximum Likelihood Classification. The result was smoothed by majority filter and converted to the vector form. Accuracy of the classification was evaluated in details, based on the check points. Overall accuracy was quite low (2001 - 82 %, 1991 - 74 %, 1979 - 67 %), depending on the quality (mainly spectral and spatial resolution) of the images and also availability of other reference data. Land cover changes for the whole time period were therefore evaluated using just the balance method (i.e. overall classes distributions in district were compared between separate years). For years 1991 and 2001 it was also tried to overlap their final vector land cover layouts, however target Change areas in...
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High temporal satellite data assimilation for vegetation spectral characteristic assignment
Malíková, Lucie ; Langhammer, Jakub (referee) ; Štych, Přemysl (advisor)
The application of high temporal satellite image data for designation of the spectral characteristic of vegetation Abstract The objektive of this paper is to evaluate possibilities of high temporal satellite data assimilation for continuous monitoring of the spectral characteristic of vegetation. There is also given the suggestion of metodology for processing MERIS data and for continuous monitoring of spectral characteristic of landscape objects. Finally, vegetation cover database for the Czech Republic in the year 2009 is created from sectorial analysis. In the paper there is used the LSU classification and thresholding of vegetation indicies histograms. The universal decision algorithm for classification of vegetation landscape component are described and particular thresholding values for the year 2009 given. The finally product of this paper is Czech vegetation cover database for the year 2009 with overall accuracy of 63,35 %. Accuracy for forest is even over 80 %. Keywords: remote sensing, MERIS, vegetation, spectral reflectance, LSU, BEAM
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Recommender System for a Dating Service
Brožovský, Lukáš ; Skopal, Tomáš (referee) ; Petříček, Václav (advisor)
The aim of the thesis is to research the utility of collaborative filtering based recommender systems in the area of dating services. The practical part of the thesis describesthe actual implementation of several standard collaborative filtering algorithms and system, which recommends potential personal matches to users based on their preferences (e.g. ratings of other user profiles). The collaborative filtering is built upon the assumption, that users with similar rating patterns will also rate alike in the future. Second part of the work focuses on several benchmarks of the implemented system's accuracy and performance on publicly available data sets (MovieLens and Jester) and also on data sets originating from real online dating services (ChceteMě and L'b'mSeTi). All benchmark results proved that collaborative filtering technique could be successfully used in the area of online dating services.
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Automatické třídění pošty pro IMAP servery
Kulič, Luboš ; Obdržálek, David (referee) ; Bednárek, David (advisor)
Number of received e-mail messages is growing explosively which forces users to classify them into several categories. In the presented work we introduce a tool called Apofis, which helps users to automate their routine every-day work with IMAP mailbox by sorting new messages for them and also by step-by-step creating of new rules based on their behaviour, while it still lets the user create, edit or delete the rules and thus affect the process of sorting and learning effectively. Two-level system of sorting rules is introduced - final rules, which the application uses to sort, and potential rules, which have been created according to user's behaviour and are waiting for more successes to prove their usability. Two potential rule learning algorithms were designed - the basic algorithm, fast but not accurate for some cases, and the advanced algorithm, which improves the accuracy by creating a new rule based on condition relevances determined from the organization of the whole mailbox. This thesis contains the most important facts and decisions about the design and implementation of the application and its algorithms.
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AI techniques in algorhitmic trading
Šmejkal, Oldřich ; Pavlíčková, Jarmila (advisor) ; Berka, Petr (referee)
Diploma thesis is focused on research and description of current state of machine learning field, focusing on methods that can be used for prediction and classification of time series, which could be then applied in the algorithmic trading field. Reading of theoretical section should explain basic principles of financial markets, algorithmic trading and machine learning also to reader, which was previously familiar with the subject only very thoroughly. Main objective of application part is to choose appropriate methods and procedures, which match current state of art techniques in machine learning field. Next step is to apply it to historical price data. Result of application of selected methods is determination of their success at out of sample data that was not used during model calibration. Success of prediction was evaluated by accuracy metric along with Sharpe ratio of basic trading strategy that is based on model predictions. Secondary outcome of this work is to explore possibilities and test usability of technologies used in application part. Specifically is tested and used SciPy environment, that combines Python with packages and tools designed for data analysis, statistics and machine learning.
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Study of Electrophysiological Function of the Heart in Experimental Cardiology
Ronzhina, Marina ; Tyšler, Milan (referee) ; Halámek, Josef (referee) ; Kolářová, Jana (advisor)
Srdeční poruchy, jejichž příkladem je ischemie myokardu, infarkt, hypertrofie levé komory a myokarditida, jsou v experimentální kardiologii obvykle studovány na modelu izolovaného srdce. Kritéria pro detekci srdečních poruch však nejsou pro zvířecí modely standardizována, což komplikuje srovnání a interpretaci výsledků různých experimentálních studií. Obzvlášť složitá situace nastává při současném výskytu několika patologických jevů, jejichž vzájemná součinnost komplikuje rozpoznání jejich individuálních účinků. Korektní posouzení stavu srdce vyžaduje také zohlednění mnoha faktorů spojených s akvizicí dat. Tato práce je věnována kvantitativnímu hodnocení elektrofyziologických změn způsobených globální ischemií myokardu. Vliv ischemie byl hodnocen pro fyziologická srdce a srdce se zvětšenou levou komorou a dále pro srdce nabarvená napěťově-citlivým barvivem di-4-ANEPPS. Přestože jsou oba fenomény často zastoupeny v animálních studiích, nebyl dosud popsán jejich vliv na manifestaci ischemie v elektrogramech (EG), ani nebyl kvantifikován jejich vliv na přesnost detekčních algoritmů pro identifikaci ischemie. Práce shrnuje kvantitativní změny srdeční funkce vyvolané ischemií (v normálních podmínkách, při hypertrofii levé komory, a při administraci barviva) založené na hodnocení EG a VKG parametrů. Dále práce obsahuje rozbor důležitých aspektů akvizice záznamů, jako je umístění snímacích elektrod, způsob výpočtu deskriptorů z EG a VKG (s použitím výsledků manuálního rozměření záznamů, nebo bez něj) a identifikace okamžiku vývoje ischemie v preparátu. Nedílnou součást práce tvoří návrh, realizace a ověření metod pro automatickou detekci ischemie v experimentálních záznamech. Výsledky práce dokazují, že dosažení opakovatelných a věrohodných výsledků je podmíněno zohledněním všech výše uvedených faktorů souvisejících jak se stavem srdce, tak s metodikou záznamu a analýzy dat.
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