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Methodological study of changes in mechanical properties of collagen tissues from pes equinovarus congenitus
Červený, Gustav ; Lopot, František (advisor) ; Eckhardt, Adam (referee)
The goal of this thesis was to invent and perform a testing protocol, witch can detect connective tissue mechanical and structural properties simultaneously in patients with clubfoot. Based on literature findings, it was presumed that connective tissue differ between medial and lateral side of foot. Because of low availability of specimen, it was important to draw and mention crucial alterations of testing protokol, for minimization of failed measurments. For reasons above, two specimens diveded into two samples was used for experiment. Described and discused methodics may enable of reader to insigt to drawbacks of this examination. For usability of this protocol, a setting and testing of few hypotheses was performed. One axis tensial testing with SHG microscopy examination was used in combination for experiment. One of the main result was finding, that structural differencies witch were expected, were not distinctive in samples in untensioned state. But, distingtive differencies may be drawed in tensioned samples. This differences for low number of specimen, cannot show any tendence. Results of tensial testing showed, that samples from medial side of foot can have higher toughness and higher fragility. For future acquisition of tendencies in specimen differencies, it is important to set particular...
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Deconvolution fluorescence microscopy of yeast cells
Štec, Tomáš ; Plášek, Jaromír (advisor) ; Heřman, Petr (referee)
Title: Deconvolution fluorescence microscopy of yeast cells Author: Tomáš Štec Department: Institute of Physics of Charles University Supervisor: prof. RNDr. Jarmoír Plášek, CSc., Institute of Physics of Charles Uni- versity Abstract: Fluorescence microscopy presents an fast and cheap alternative to more advanced imaging methods like confocal and electron microscopy, even though it is subject to heavy image distortion. It is possible to recover most of the original distortion-free image using deconvolution in computer image processing. This al- lows reconstruction of 3D structure of studied objects. Deconvolution procedure of NIS Elements AR program undergoes an thorough inspection in this diploma the- sis. It is then applied on restoration of 3D structure of calcofluor stained cell wall of budding yeast Saccharomyces cerevisiae. Changes of the structure of the cell wall during cell ageing are being examined. Cell wall of aged cells shows increased surface roughness and even ruptures at the end of cell life. Keywords: fluorescence, microscopy, deconvolution, NIS Elements AR, calcofluor, yeast, cell wall, ageing
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ASR potential of quartz in experimental mortar bar specimens
Kuchyňová, Markéta ; Kuchařová, Aneta (advisor) ; Vavro, Martin (referee)
The alkali-silica reaction is one of the most damaging chemical reactions taking place in concrete, which can cause fatal damage. ASR originates under following conditions: high moisture (> 80 %), sufficient amount of alkaline ions (Ca2+ , Na+ , K+ ) and use of reactive aggregates (low crystaline or deformed quartz, amorphous SiO2). Reactive aggretates react with high alkaline pore solution and produce hydrofile gels. These gels absorb water and swell. Dilatometric test methods are commonly used to evaluate the reactivity of aggregates. The principle of dilatometric test methods is simple. Mortar or concrete prisms are created in a laboratory, then they are stored in the special environment, which accelerates the inception of ASR. The creation and expansion of alkali-silica gels cause prism's length changes. The major goal of this diploma thesis was to evaluate the alkali-silica reactivity potential of quartz-rich rocks using microscopic (polarizing microscopy, scanning electron microscopy combined with SEM/BSE image analysis) and dilatometric (ASTM C1260, RILEM AAR-4.1) methods. Rocks were assessed as reactive, potentially reactive and non-reactive by the ASTM C1260 method. The reactivity of aggregates was connected with the amount of cryptocrystaline matrix, grain size, shape of grain boundaries,...
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Microscopy of leaves from different Bergenia species.
Rulfová, Kateřina ; Spilková, Jiřina (advisor) ; Kašparová, Marie (referee)
Plants of the genus Bergenia are part of remedies used in Ayurveda medicine. They also play an important part in traditional healing practice in China, India, Mongolia and Russia. Theoretical part of this thesis sums the newest findings and research results concerning three Bergenia species: Bergenia ciliata (Haw.) Sternb., Bergenia crassifolia (L.) Fritsch a Bergenia ornata Stein. The thesis mainly focuses on their current and potential use in medicine and pharmacy. Bergenia extract is traditionally used for dissolving kidney stones, treating respiratory tract illnesses and to stop bleeding. The most important active substances of these plants, their characteristics and main effects are also noted. Bergenia is an important source of arbutin and bergenin. Bergenin has antitussive, antiflogistic and gastroprotective effects. Arbutin is used to treat urinary tract diseases and in cosmetology to lighten the skin. The experimental part of this thesis includes methods of preparation of permanent microscope slides from leaves of chosen Bergenia species. Photographs have been taken from both permanent and native slides. Anatomy of the leaf and leaf epidermis is described including stomatal index. Presence of calcium oxalate crystals in form of druses is also documented. Basic anatomical features were...
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Microscopic characterization of flowers from culivars of Sambucus nigra L.
Andělová, Veronika ; Spilková, Jiřina (advisor) ; Kašparová, Marie (referee)
Sambucus Nigra belongs to the most favourite plants of our national folk medicine. It has important place in pharmacy and food industry. It is know in our environment mainly in wild form however increasing interest and demand for qualitative balanced drug contributed to cultivation of cultural varieties in plantations. Flower of Sambucus Nigra contains mostly flavonoids. For receiving of the most quality drug is necessary to follow the rules of harvesting and drying. Currently is drug obtained only just from the plants in wild nature. The goal of thesis was valorising of all characteristic features of flowers Sambacus Nigra and differentiation between cultural varieties and wild forms with using microscopic test of identity stated in Czech Pharmacopoeia 2009. Characteristic flower features of Albida, Alleső, Aurea, Bohatka, Dana, Haschberg, Juicy, Kőrsőr, Madona, Pregarten, Riese aus Vobloch, Tulbing and wild form of Sambuca Nigra were examined. All samples were brightened in solution of chloral hydrate and afterwards microscopic preparations were created. Results were photographically documented. Among the observed features belongs: cells containing sand of calcium oxalate, stomata and epidermal cells of crown leaf. Besides were observed pollen grains, flowers venation or cuticle corrugation of...
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Laboratory diagnosis of dermatophytosis
Drnková, Nela ; Vejsová, Marcela (advisor) ; Křivčíková, Lucie (referee)
Author: Nela Drnková Title of the bachelor thesis: Laboratory diagnosis of dermatophytosis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Degree course: Medical laboratory technician My bachelor thesis is focused on laboratory diagnosis of dermatophytes. The purpose of this work is created summary of laboratory methods, which are used for diagnosis of dermatophytosis. Beginning of thesis is given to common characteristics of dermatophytes, taxonomony, nomenclature and morphology of individual genera. The big part of thesis creates summary of laboratory methods. There are described individual types of microscopy, cultural techniques, molecular methods and immunological methods. The end of the thesis is dealted with infections, which are caused by dermatophytes and their treatment. Light microscopy and culture are used most frequently. Key words: dermatophytes, Trichophyton, Microsporum, Epidermophyton, laboratory diagnosis, tinea
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Lithic function and its application in archeology
Krásná, Soňa ; Popelka, Miroslav (advisor) ; Květina, Petr (referee) ; Hroníková, Linda (referee)
The goal of the thesis is to find the way how to apply use-wear analysis as well as functional analysis to archaeological assemblages of selected artefacts from Central European archaeological contexts, namely lithics (chipped stone artefacts) and obtain the greatest potential from the analysis. Thesis consists of: current state in the field of functional studies research worldwide, method of use-wear application in connection with material science knowledge, especially tribology. Use-wear analysis is applied to the selected lithic artefacts from Paleolithic to Eneolithic Periods. The results of this work are based on the following microscopic approaches: low power approach (LPA), high power approach (HPA), scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). There are described and stated differences in potential of above mentioned approaches in connection with specific archaeological artefacts (assemblages of artefacts). The question answered in the conclusion is how to apply the above mentioned methodological approaches in application to various archaeological materials (period, number, context etc.) to obtain the greatest informational potential from the material analysed. Work is concluded with specific terminology from the field of tribology and use-wear analysis...
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Microscopy of Sambuci fructus from cultivars of Sambucus nigra.
Applová, Lenka ; Spilková, Jiřina (advisor) ; Kašparová, Marie (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Lenka Applová Consultant: doc. Dr. Jiřina Spilková, CSc. Microscopy of Sambuci fructus from cultivars of Sambucus nigra Sambucus nigra L. is the important plant of the traditional folk medicine, its knowledge is accepted in the pharmaceutical and food industry. The elderberry fruits are used as diaphoretic, diuretic, during the migraine and neurological difficulties. On the grounds of the rising demand the collection of the wild-growing berries do not suffice and that is the reason for growing the elderberry and its cultivated varieties in plantations. The cultivated varieties of elderberry, which are grown for the use of the fruits in the food industry, could be exactly provided the quality drug for pharmaceutical use. The thesis is focused on the research of the microscopic characteristics of the elderberries fruits wild-growing elderberry and its cultivated varieties Allesö, Bohatka, Haschberg, Mamut, Sambo, Sambu, Samdal, Sampo, Samyl and Weihenstephan, which originated from the Research and Breeding Institute of Pomology Holovousy Ltd. The berries of the wild- growing elderberry were obtained in the Botanical Garden of Medicinal Herbs of Faculty of Pharmacy in Hradec Králové. The...
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Imaging of fluorescently labelled mitochondria using Biplane_FPALM microscopy
Dostál, Marek ; Plášek, Jaromír (advisor) ; Valenta, Jan (referee)
Title: Imaging of fluorescently labelled mitochondria using Biplane FPALM microscopy Author: Bc. Marek Dostál Department: Fyzikální ústav UK Supervisor: prof. RNDr. Jaromír Plášek, CSc. Consultants: RNDr. Petr Ježek, DrSc. Mgr. Hana Engstová, Ph.D. Abstract: In this thesis the results are presented of a search for suitable PCFP markers for a visualization inner and outer mitochondrial membranes by unique biplane FPALM microscope. We participated in debugging the measuring software for the purpose of 3D vizualization mitochondrial nets and determine their parameters. For this purpose were developed two methods. The first one can be applied to determine the parametrs of outer membranes and the second one is applicable to determine parametrs of inner membranes. On smaller statistical file we managed to confirm dependence quality of mitochondrial net on the cell culture conditions. Under stress conditions e.g. hypoxia the quality of mitochondrial net is changed. Keywords: mitochondria, Biplane FPALM, GFP, 3D visualization.
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