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Genetic factors of resistance to chemotherapy and clinical characteristics of breast tumors.
Kunická, Tereza ; Souček, Pavel (advisor) ; Kleibl, Zdeněk (referee)
Breast cancer is the most frequent malignancy in women population both in the Czech Republic and worldwide. Treatment of this disease involves surgical removal of the tumor, radiotherapy, chemotherapy and hormonal therapy. Recently, targeted biological treatment is also approached. Each patient reacts to the treatment individually and thus high variability in response is common. Multidrug resistance (MDR) presents one of the most important obstacles to successful chemotherapy. MDR is often associated with a decreased intracellular accumulation of anticancer drugs and an increased expression of ABC transporters such asABCC1 of our interest. The ABC family of membrane transport proteins includes the well-known mediators of resistance to anticancer drugs. In particular, ABCB1, ABCC1 and ABCG2 actively perform efflux of various types of drugs from cancer cells, thereby conferring resistance to those agents. The main aim of this study was to asses the genetic variability of the ABCC1 gene in 191 patients with breast cancer and to determinate the expression profile of ABCC1 in 30 patients from this cohort who were treated preoperatively. We evaluated relations between ABCC1 genotype, or phenotype and prognostic factors including the result of chemotherapy. Gene expression was measured in preamplificated...
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Unlimited Evolutionary Growth of the Computational Power of Self-Reproducing Automata and Other Results
Wiedermann, Jiří
We design two original computational models - viz. a globular universe and the autopoietic automata - capturing essential features of evolution: the construction of self-reproducing evolutionary automata via self-assembly and genetic information transfer from the parents to their offsprings. Within this framework we show the unlimited growth of the computational power of automata during their evolution and we characterize the power of lineages of such automata with the help of interactive Turing machines.
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Heuristic and metaheuristic methods for travelling salesman problem
Burdová, Jana ; Kalčevová, Jana (advisor) ; Zouhar, Jan (referee)
Minimal length of a travelling salesman's problem had been studied in this diploma these. Travelling salesman must come trough each place just once and then go back to the starting place. This problem can be illustrated as a problem of graph theory, such that places are the vertices, roads are the edges, distances of roads are the lengths of edges. The optimal travelling salesman's problem tour is the shortest Hamiltionian cycle in the graph. It is a classical NP-complete problem. There is no algorithm that solves this problem in polynomial time. This problem can be solved by using various approximation algorithms, they offer less time consumption and lowest quality than optimization. This diploma these had been dedicated to approximation algorithms, for example: nearest neighbor method, minimal spanning tree method, Christofide's method, 2-opt., genetic algorithm, etc.
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The problematics of the deception detection in the forensic psychology
Mynaříková, Lenka ; Slaměník, Ivan (referee) ; Boukalová, Hedvika (advisor)
This diploma thesis called 'The problematics of the deception detection in the forensic psychology' deals with lying and lies from the viewpoint of its types, functions and considering the development of an individual and it describes the role of lying in our lives. Then it examines the integration of the deception detection into the forensic psychology, the description of the forensic psychology itself and it tries to specify the differences between laypersons and proffesionals in their ability to detect lies. The thesis describes the particular approaches to the detection of deception from its historical beginning to contemporary methods, such as psychophysiological and verbal tools of lie detection, observing of someone's behaviour or modern alternative methods, such as lie detection via handwriting or drawing, whose research has just started. The thesis explores also some other aspects, that can influence the deception detection, namely false memories, false confessions and the suggestive style of conducting the interrogation.
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Evolutionary Design of Artificial Neural Network
Jílek, Tomáš ; Šperka, Svatopluk (referee) ; Pospíchal, Petr (advisor)
Focus of this bachelor thesis is on Neural networks, Genetic algorithms and Evolutionary Design. First part of thesis describes Neural networks, their history, training and ways of use and Genetic algorithms, their components, operators and practical application. Next part is devoted to prediction of time series, specifically prediction with use of Neural networks. This is followed by practical part of work, implementation of experiments with Evolutionary design of Neural networks for prediction in which currency exchange rates of several countries are used as a predicted time series. Results and discussion about further development of thesis are described in last chapter.
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Electrospinning of Modified Biopolymers for Medical Applications
Pavliňáková, Veronika ; Martinová,, Lenka (referee) ; Zajíčková, Lenka (referee) ; Vojtová, Lucy (advisor)
Předkládaná disertační práce se zabývá přípravou a charakterizací nových biokompatibilních nanovláken s potenciální aplikací v medicíně. V této práci byl výběr jednotlivých složek pro přípravu nanovlákenného materiálu zvolen tak, aby vyhovoval nárokům tkáňového inženýrství. Literární rešerše shrnuje poznatky o elektrostatickém zvlákňování a o jeho parametrech. Dále se věnuje možnostem elektrostatického zvlákňování proteinů kolagenu a želatiny a jejich směsmi se syntetickými polymery a biopolymery a anorganickými plnivy. Teoretická část řeší také různé postupy síťování nanovláken vedoucí ke zlepšení jejich hydrolytické stability a mechanických vlastností. Poslední část je zaměřena na anorganické nanotrubky halloysitu (HNT), které získaly svou pozornost díky svým vynikajícím fyzikálním a biologickým vlastnostem. V experimentální části byly zpracovány dvě případové studie, z nichž každá se zabývá přípravou nanovlákenných biomateriálů s potenciální aplikací v medicíně. První studie je zaměřena na přípravu a charakterizaci nových hydrolyticky stabilních antibakteriálních želatinových nanovláken modifikovaných pomocí oxidované celulózy. Unikátní inhibiční účinky nanovláken byly testovány na kmenu bakterie Escherichia coli pomocí metody chemické bioluminiscence. Kultivované buňky lidského papilárního adenokacinomu plic prokázaly dobrou adhezi a proliferaci k povrchu nanovláken. Druhá část popisuje vliv zdroje a množství anorganických halloysitových nanotrubek na strukturu a vlastnosti amfifilních nanovláken ze směsi želatiny a syntetického polykaprolaktonu. Přídavek HNT zlepšil tepelnou stabilitu, mechanické vlastnosti (jak tuhost, tak prodloužení) a snížil krystalinitu nanovláken. HNT z různých zdrojů neměl vliv na chování buněk, ale mírně ovlivnil proliferaci a životaschopnost buněk na povrchu nanovláken.
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Digital forensic anthropology and sexual dimorphism of recent population os coxae: implication for sex estimation.
Mesteková, Šárka ; Brůžek, Jaroslav (advisor) ; Dobisíková, Miluše (referee)
This thesis uses the metric evaluation of 3D models created from CT images. The study is based on an examination of 51 males and 55 females CT scans from recent European population and also based on a metric data (10 linear measurements) of the same geographical provenance of the mid-20th century (n=113). The purpose of our research project is to determine the degree of sexual dimorphism in current population and compare the degree of sexual dimorphism in this geographic area in the middle of the last century. Intraobserver variabilities of linear measurements were less than 2%. Both groups were statistically tested. An index of sexual dimorphism (ISD) was used to assess the level of sexual dimorphism within each sample (Paris ISD = 8,28, Marseille ISD = 6,50). The two-sided t-test indicates that the degree of sexual dimorphism is not significantly different between population from the mid-20th century and recent population (p = 0,680). The secular trend was expressed by the z-score. The results showed that changes in the pelvic bone between the two groups are not significant, since neither one of the measurements does not exceed ±2 SD. Finally, we verified the reliability of method DSP ("Diagnose Sexuelle Probabiliste") for measurements deducted from CT-derived models. The results showed the...
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The study of genetic changes of children patients suffering from the acute lymphoblastic leukemia (ALL) using mFISH / mBAND and micro-arrays.
Bártů, Linda ; Zemanová, Zuzana (advisor) ; Březinová, Jana (referee)
Acute lymphoblastic leukemia is the most common malignancy in children. The most important examination at the time of diagnosis includes karyotype of leukemic cells which divides patients into prognostic groups according to cytogenetic finding. In up to 90 % of patients the chromosomal aberrations with well known clinical significance are designated. One of cytogenetic type is high hyperdiploid ALL (51-68 chromosomes) associated with favorable prognosis. Nevertheless, relapses of the disease occur even in these children. One possible reason why this happens could be an increased genomic instability of leukemic cells that causes cryptic structural rearrangements. In a retrospective study, we examined a total of 232 children with newly diagnosed B-ALL using conventional cytogenetic analyses and interphase fluorescence in situ hybridization (I-FISH) with a panel of DNA probes (Abbott Vysis) in order to detect heteroploid cells. In patients with suspect cryptic structural chromosome aberrations, we analyzed the karyotypes in detail by multicolor FISH and multicolor banding (mFISH/mBAND; MetaSystems). The extent of aberrations was determined by comparative genomic hybridization on BAC arrays (array CGH; BlueGnome). Cell clones with high hyperdiploid karyotype were detected in a total of 102 children (44 %). In...
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Speciation of the genus Lethrus (Coleoptera: Geotrupidae) of the eastern mediterranean
Drožová, Dana ; Janšta, Petr (advisor) ; Bezděk, Aleš (referee)
Earth-boring dung beetles of the genus Lethrus Scopoli, 1777 belong to the family Geotrupidae, classified into three subfamilies (Geotrupinae, Lethrinae a Taurocerastinae). The description of about 120 species, divided into nine subgenera, is based on morphology and geographic distribution. Shape of mandibles, ventral mandible processes, pronotum and structure of external male genitalia are used as diagnostic morphological characters for taxa. The richest species diversity is known from Central Asia. The distribution area reaches, in the east, up to Mongolia and China, and in the west, up to Southeast Europe. All species are robust, relatively large flightless beetles with low dispersial ability. They feed on parts of fresh plants instead of feces like other dung beetles. Master thesis is focused on the species distributed in the Eastern Mediterranean and Central Europe. This area is inhabited only by nominotypical subgenus Lethrus. The main goal of thesis is to clear up phylogenetic relationships between species of subgenus and speciation events using molecular genetic methods. We have examined 91 samples of the genus Lethrus using two mitochondrial genes - cytochrome b (382 bp, 80 sequences), cytochrome oxidase I (815 bp, 87 sequences) and nuclear gene 28S rDNA (D2-D5) (1100 bp, 11 sequences)....
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Evolutionary algorithms
Haupt, Daniel ; Polách, Petr (referee) ; Honzík, Petr (advisor)
The first part of this work deals with the optimization and evolutionary algorithms which are used as a tool to solve complex optimization problems. The discussed algorithms are Differential Evolution, Genetic Algorithm, Simulated Annealing and deterministic non-evolutionary algorithm Taboo Search.. Consequently the discussion is held on the issue of testing the optimization algorithms through the use of the test function gallery and comparison solution all algorithms on Travelling salesman problem. In the second part of this work all above mentioned optimization algorithms are tested on 11 test functions and on three models of placement cities in Travelling salesman problem. Firstly, the experiments are carried out with unlimited number of accesses to the fitness function and secondly with limited number of accesses to the fitness function. All the data are processed statistically and graphically.
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