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FISH method and its use in protistology
Frenclová, Martina ; Hampl, Vladimír (advisor) ; Zubáčová, Zuzana (referee)
Fluorescence in situ hybridization (FISH) is a technique that allows the localization and identification of specific sequences of nucleotides in DNA or RNA, which is subsequently visible under the microscope. FISH involves first denaturing the nucleic acids, either using high temperatures or by treatment with denaturing agents such as formamide.After subsequent induction of reasociation, the examined DNA or RNA pairs according to the complementarity rules with the short molecule called the probe, this process is called hybridization. Hybridization occurs in situ, that is within the examined specimen. Probes can be labeled either directly using fluorophores, or indirectly with a hapten, which is a substance having antigenic properties, which is subsequently detected using labeled antibodies or streptavidine. FISH has a large number of applications in molecular biology and medical science. In laboratory research in protistolgii FISH can be used for example to map the chromosomal genes to study the evolution of genome, analysis of nuclear organization or to confirmation of the origin of DNA sequence. Key words: FISH, fluorescence, probe, cell identification, environmental studies
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Industrial culture, photography and child
Blažková, Františka ; Šmíd, Jan (advisor) ; Sedlák, Michal (referee)
Thesis tracks historical, social and artistic aspects of industrial culture, with focus on two main fields: architecture and photography. Thesis also includes a brief chapter about industrial music and interview with Franz Trechler, who is a famous musician, for completion. The outset of industrial architecture, its development and revitalization, placement in contemporary culture are the stressed themes. There is also focus on the artists who engaged/are engaging in industrial painting and photography. Apart from overview of aforementioned topics thesis also includes an opinion of unbiased child observer on industrial architecture and photography. These two are combined in a work of applied art consisting of collage of photographs with added mixed media. Complementary questionnaire was also part of the research. The group of children consists of three different classes so various ages could be represented. As a conclusion, thesis compares the works with corresponding parts of the RVP and rlooks into the hypothesis, that artwork on previously unknown topic can be succesfully fulfied. Original author's artwork consists of series of surreal collages using photographs, which are completed with drawing and where the compositions were inspired by industrial music.
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Culture of industrial, phoptpgraphy and child
Blažková, Františka ; Šmíd, Jan (advisor) ; Raudenský, Martin (referee)
from Bacheor thesis Františka Blažková THE INDUSTRIAL CULTURE, PHOTOGRAPHY AND CHILD červen 2013 In this bachelor thesis is thoroughly described a phenomenon of industrial culture with focus on two main fields: architecture and music scene. These two branches of the topic have the best correlation in the sense of architecture as a prototype and music as a mirrored image of architecture. This thesis is mapping the history if industrial architecture and its connection to history of industrial music. Besides the gathering of data concerning this phenomenon and coming to conclusions based on them this thesis also provides an unbiased opinion of the child observer, which is necessary to complete the image. The age of the children was picked according to their socio-cultural knowledge and none or partial knowledge of the topic of industrial architecture. The research was carried out as a questionnaire with attached creative task, based on the reproductions of the photographs taken especially for this thesis.
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Verification technology BioSealing by EIS method
Fránková, Hana ; Novotná, Jitka (referee) ; Pařílková, Jana (advisor)
Diploma thesis is dealing with recognition of Biosealing technology using electrical impedance spektrometry method. BioSealing is an innovative method of soil body reconstruction, which uses stimulation of soil bacterial activity to seal the leakage. The method was developed in the Netherlands and it was used in conditions of the Czech Republic in years 2015 and 2016 on the Hornice water work. This earth dam was monitored by electrical impedance spectrometry by apparatus with device Z-meter IV in this period and the data are processed and assessed in this diploma thesis.
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Diagnosis of reinforced concrete buildings with the system of elevated ceilings
Čeřovský, Martin ; Láník, Jaromír (referee) ; Žítt, Petr (advisor)
This thesis is focused on the diagnostics of reinforced concrete frame structures with the used system of elevated ceilings. The first part of the thesis is devoted to the issue of the methods of design and construction of these objects. The following chapter describes selected methods leading to determination of the material characteristics and to the specification of the constructional system. In its conclusion, the thesis deals with the appropriate design of implementation of the preliminary and detailed exploration of the structures with the used system of elevated ceilings.
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Extraction of Available Information from SSH Protocol Headers
Ďurčanský, Norbert ; Bartoš, Václav (referee) ; Kořenek, Jan (advisor)
This paper analyzes issue regarding to extraction of available information from SSH protocol. To achieve this aim, knowledge about SSH protocol were used to implement plugin for FlowMon exporter. During the testing plugin was tested on real network and validated in terms of stability, efficiency and accuracy. The result plugin allows us to extract information from SSH protocol and futher analysis without decryption of traffic.
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Design and Realization of Phantom for MRI
Šablatura, Martin ; Čáp, Martin (referee) ; Nešpor, Dušan (advisor)
Paper studies the principles of MRI and composition of MR system. Main part of thesis focuses on design, mixing and measurement of solution for MR system phantom. Teoretical part of paper focuses on problematic of MRI and phantoms for MR system, including description of specific phantom, which is then realised. Paper contains methods for designing proposed kind of phantom and its followed measurement. Part of paper also focuses on mathematical models of elaboration of data aquired from measurements.
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Vocabulary and its development in children aged 8 to 10
NĚMCOVÁ, Věra
Diploma work dealed with vocabulary and its development in children aged 8 to 10. The research was doing as a language tests, language games and essays during the longer time period. The target of this work is found out if the vocabulary is more developed. We examinated also children from another ethnic groups and children with developmental disorders.
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Probe for RFID cards tests at 13.56 MHz
Zigo, Viktor ; Vychodil, Josef (referee) ; Derbek, Vojtěch (advisor)
This thesis deals with RFID technology and the tag design for RFID device testing on the frequency of 13, 56 MHz. Everything regarding testing of such devices is described in ISO 18047-3 Standard. The target of this thesis is to design and construct the impedance matching (probe) between a proof coil and SW radio USRP N200 and the principles of this probe design are explained.
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