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Map of the Vypustek Cave Surroundings - southern part
Stískalová, Alena ; Fišer, Zdeněk (referee) ; Weigel, Josef (advisor)
This thesis is about mapping of the southern surroundings of the area caves Výpustek in the ratio scale 1:500 with the schematic mapping of the subterranean part of the cave. The theoretical part is devoted to the history of this cave and creating maps. The practical part contains calculations, creation of maps and representation of vertical cuts. The outcome is the sketch of the position and diagrammatical sketch of the existed locality and vertical cuts.
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Detection of microorganisms in the soil using CARD-FISH (Catalyzed Reporter Deposition - Fluorescence in situ hybridization)
Krištůfek, Václav ; Kasalický, Vojtěch ; Chroňáková, Alica ; Elhottová, Dana ; Němec, Jan
CARD-FISH protocol (Catalyzed Reporter Deposition - Fluorescence in situ Hybridization) for detection of soil microbial community composition was successfully established in the Institute of Soil Biology. CARD-FISH is the relatively new molecular biology tool permitting to quantify distinct groups of microorganisms by hybridization of specific oligonucleotide probes. Compared to classic FISH-protocol the probe-attached enzyme amplifies the fluorescent signal and enables to detect nearly all microorganisms in soil or in organic compounds. As the first, we used this method to determine the bacterial community composition of a dated layer of guano-heap from Domica Cave (Slovak Carst National Park, Slovakia). We quantified the percentuel composition of Archaea, Eubacteria (.i.Planktomycetales, Verrucomicrobiales./i. included) and major bacterial groups (Actinobacteria, proteobacteria, .i.Cytophaga-Flavobacter./i.-Bacteroidetes, Sphingobacteria).
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Microscopic fungi as food of cave invertebrate animals - a laboratory food preference experiment
Nováková, Alena ; Luptáčik, P. ; Kováč, L. ; Lukešová, Alena ; Šustr, Vladimír
The aim was to improve the methodology of the multi-choice food preference tests as a tool for investigation of feeding preference of cave invertebrates. Fungi and algae isolated from cave sediments of the Domica Cave system (NP Slovak Karst) were offered in cafeteria tests as a food to springtail .i.Folsomia candida./i., mites .i.Pantelozetes cavatica, Scheloribates pallidulus./i., and isopod .i.Mesoniscus graniger. M. graniger./i. preferred some species of algae, all offered fungi were rejected. Mainly algae and .i.Mucor./i. spp. from microscopic fungi are preferred by .i.S. pallidulus./i.. Preference of .i.Mucor./i. spp. was observed by cave mite .i.P. cavatica./i. and cave population of .i.F. candida./i. too. All cave animals showed low preference for offered food and have tendency to stay rather on clay cave sediment then on the food.
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Study of stagnant water occurrences and dripping waters in caves: Chemical composition and remarks to corrosion of dripstones
Zeman, Ondřej ; Bruthans, J. ; Filippi, Michal ; Vysoká, H.
Slight stagnant water occurrences (drops) were studied in four caves in the Czech Republic and Slovakia. Major components were analyzed (Na+, K+, Ca2+, Mg2+, SO42-, Cl-, NO3-), pH, conductivity and temperature were measured in the field. Results are shown in table 1. Waters coming from condensation differs by higher amount of sulphate and some other components if compared with dripping waters.
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Environmental tracers (18O, 3H, CFCs, SF6) exploitation for study of mean residence time and flow pattern of dripping water and karst springs (Moravian Karst)
Bruthans, J. ; Zeman, Ondřej ; Jež, M. ; Himmel, J. ; Buzek, F. ; Melková, J. ; Oster, H. ; Geršl, M.
Mean residence time and flow pattern was studied in unsaturated zone of Ochoz Cave in southern part of Moravian Karst by means of 18O in the years 2001 to 2003. The results of study showed relatively long residence time in the unsaturated zone (about 50% komponent with residence time exceeding one year). The proportion components coming from different reservoirs were computed from two components (soil/epikarst; meltwater). The results clearly indicate, that more than 50% of water (nearly 100% in 2003) in dripping waters was derived from soil/epikarst water, which originate from (late) summer precipitations (heavy 18O content)! It means that infiltrating snowmelt water was expelling (by piston flow effect) the summer water from soil and/or epikarst. Water from snowmelt was then lost by evapotranspiration in summer time. If this effect is common in Langer period, it can change composition of d18O in dripping waters and hence also in dripstones precipitating from these waters.
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Analysis of visit rate caves
Drbalová, Pavla ; Musil, Martin (advisor) ; Přibyl, Lubomír (referee)
The study aims to provide the visit rate analysis of the accessible caves in the Czech Republic. The study evaluates and parses the visit rate from time and other indicators point of view. One of the targets is also to judge, whether the reconstruction of the caves influences the visit rate.
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