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Encapsulation of active components and their applications in food industry
Malčíková, Hana ; Němcová, Andrea (referee) ; Skoumalová, Petra (advisor)
The Diploma thesis is focused on encapsulation of bioactive compounds which are contained in selected superfoods for the purpose of application to childrens nutrition supplements. In view of the nature of selected samples, which are seaweed, walnuts, hemp seed and flax seed, the emphasis is put on the content of omega-3 and omega-6 acids. The theoretical part introduces topic of children food, further it describes the nature of selected superfoods and last but not least it contains screening of available childrens food supplements containing omega-3 fatty acids on the market. In the experimental part, an optimization of the appropriate type of extract was made. A 24 hour aqueous macerate and 20% ethanol macerate were selected for next analyzes. Samples were characterized by content of polyphenols, flavonoids and the antioxidant activity and they were used to forming of extracts were encapsulated into liposomes for which their encapsulation efficiency, stability and long-term stability have been tested. Hexan extracts were also prepared for the same reasons. It was found that liposomes of selected superfoods should not be stored for 12 weeks. The samples were used analyzed for the carbohydrate content by the duboise method, a protein content by the Kjeldahl method and the determination of the fatty acid profile by gas chromatography. Furthermore, the content of carotenoids and chlorophylls in algae samples was determined by spectrophotometry and this analysis was verified by high-performance liquid chromatography. The technique of high-performance liquid chromatography was also used to verified a content of polyphenols in the algae samples. Finally, a sensory analysis of prepared alginate supplements containing algae and cold-pressed oils was performer in combinations to achieve a higher omega-3 and omega-6 fatty acid content. The F01-labeled sample containing wakame algae was evaluated as the best one.
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Hranice – redefinition of urban structure
Uherková, Ivona ; Ryšavá, Hana (referee) ; Ponešová, Barbora (advisor)
The diploma thesis Park and Ride deals with the design of a car park in the town of Hranice, which is known mainly for its important transport train node and Hranice Abyss. The city now suffers from the high intensity of car traffic in the city center due to insufficient street network. This network is disrupted by a four-lane road that was built in the 1980s and cut natural transport links. The abolition of the four-lane road, which is now lacking in transport services, releases a large amount of development area, which is primarily used as an agri-recreational area. Part of this green belt is the parking house, which prevents the entry of surplus cars into the city center. The parking house is a new transport hub of the city, which also includes elements of alternative transport - transfer to public transport or bicycle, which makes it possible to create a city center pleasant for pedestrians.
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Use of algae for production of biomass and bioproducts
Slonek, Jaroslav ; Máša, Vítězslav (referee) ; Brummer, Vladimír (advisor)
Submitted bachelor thesis is focused on description of use of algae for biomass and bioproducts production. The first part of the thesis is devoted to the taxonomic classification of algae and a basic summary of the characteristics of the different algal groups. The next part is aimed at bioproducts, which are obtained by algae cultivation and possibilities of further processing for energy purposes are also described. As for the cultivation, the emphasis is on modern methods in this area, i.e. on the possibility of using waste products in the culture process. In the next part, technological parameters of cultivation are discussed, for instance effects of technological parameters on growth and composition of biomass and production of desired products. The next chapter summarizes types of used culture bioreactors in which the growth of biomass occurs in a controlled environment. The information obtained and collected in the thesis is further discussed and summarized in the conclusion.
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Isolation of active compounds from algae and their application in cosmetic products
Nováková, Laura ; Byrtusová, Dana (referee) ; Skoumalová, Petra (advisor)
The bachelor thesis is dedicated to the study of active substances and antioxidant effects of cultivated microalgae. We compared the results with a commercial alga that is freely available. In the theoretical part we focused on description of algal metabolites in cosmetics and a brief description of the methods used in their testing. In the experimental part there were analyzed three types of extracts (aqueous, alcohol and oil) from 4 species of algae. It is mainly the content of polyphenols, flavonoids and antioxidant activity that is determined. According to the results, the highest content of active substances was found in oil extracts. All extracts were encapsulated into liposomes. The average size of the nanoparticles was in range of 200 nm to 300 nm. The particles were stable enough also for long-term stability. The largest decrease was observed in the oil extract of the cultivated algae Chlorella vulgaris. The cytotoxicity of oil extracts was realized by MTT test on human cell cultures HaCaT. Finally, we concluded that the prepared liposomal particles could be used for anti-aging cosmetics.
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The evolution of auxin metabolism, signalling and transport in the green lineage (Viridiplantae)
Schmidt, Vojtěch ; Petrášek, Jan (advisor) ; Fendrych, Matyáš (referee)
Phytohormone auxin is an essential coordinator of growth and development within land- plants. Mechanisms of auxin action in algae is still poorly understood, given that related research has begun recently. Current progress is derived mainly from available sequence data, which are necessary for analysis of gene homology with higher plants. In this case with the genes related to auxin action mechanisms, e.g. metabolism, signaling and transport. Auxin biosynthesis predated the split of Chlorophyta and Streptophyta, but tighter regulation of auxin levels is the matter of land-plants. First auxin responses were probably non-transcriptional. Transcription-regulating nuclear signaling pathway assembled from more ancient domains, some of them were identified in Charophyta, but whole pathway was complete after colonization of land. Auxin transport out of the cell was one of the original mechanisms of regulating its levels. More sophisticated auxin carrier system was proposed to evolve in Charophyta, where polar auxin transport was identified. Auxin as a signaling molecule was present in primitive algae groups, but its response spectrum dramatically increased after colonization of land. Future research of auxin action evolution needs more model organisms to be established, especially from Charophyta....
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Studijní materiály pro badatelsky orientované vyučování k tématu sinic a řas na středních školách
HNÁTEK, David
Introduction of the thesis deals with inquiry-based science learning - from the level of research to its evaluation. The following practical advice summarize information about the possibilities of obtaining natural material for teaching, creating permanent diatom preparations and examples of suitable determination literature for teachers. The main part of this work presents 12 elaborated study materials for research teaching method divided into five phycologically oriented topics: (1) cyanobacterial and algal physiology, (2) biodiversity of standing waters, (3) algae defense mechanisms, (4) cyanobacteria and algae like bioindicators, (5) substances in cyanobacteria and algae and their importance for humans. Individual study materials contain information on the level of research, methodological guidance for teachers with a theoretical basis and introductory questions to motivate students, as well as a recording sheet for students and, in conclusion, own proposed solution for teachers. Another part of this thesis is a comparison of inquiry-based science learning with frontal education, which is done by didactic test.
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The issue of biotic attack of ETICS
Těžká, Adéla ; Žák,, Antonín (referee) ; Žižková, Nikol (advisor)
This bachelor thesis deals with the issue of biotic attack of ETICS contact insulation systems. The theoretical part is focused on the individual components of ETICS, both from the point of view of materials and from the point of view of individual steps. In addition, there are organisms that cause biotic attack on facades. The practical part deals with the possibilities of testing the resistance of the surface treatment of ETICS against biotic attack, both in laboratory conditions and in the exterior.
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Floristicko-ekologická studie oblasti Bystřicka se zaměřením na ekologii zelených volvokálních řas
HNÁTEK, David
Since 1889 twenty-two different species of colonial algae from the order Volvocales have been found in the Czech Republic according to the available algological literature. In this work the morphology and ecology of these species is described in detail. Besides investigating the literature resources, I performed my own floristic research of twenty ponds near Bystřice u Benešova. Phytoplankton samples from these ponds were taken in spring, summer, and autumn of years 2014 and 2015 using phytoplankton net and at the same time the environmental characteristics such as pH, conductivity, water transparency, temperature, and degree of shading were measured. Cyanobacteria and algae in the samples were identified to the lowest possible taxonomic level and their relative abundance was assessed. The differences in species composition between ponds and between seasons were compared using basic statistical methods and relationship between environmental factors and species composition was studied.
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Production of lipids and lipid-soluble compounds by some yeasts and algae
Šimanský, Samuel ; Rapta, Marek (referee) ; Márová, Ivana (advisor)
The submitted bachelor thesis focuses on production of lipids and lipid-soluble compounds by selected strains of carotenogenic yeasts, autotrophic algae and cyanobacteria. Different forms of stress were applied on selected strains and its influence was subsequently compared. The theoretical part deals with the processing of knowledge about selected strains of yeast, algae and cyanobacteria. There are also mentioned the selected metabolites (lipids, carotenoids, ergosterol, ubiquinone and chlorophyll) and the possibilities of their determination. The experimental part deals primarily with the influence of nutritional stress on the production of selected metabolites and the yeast biomass, namely their growth properties on waste fat. Furthermore, there was monitored the effect of elevated and reduced temperature on the cultivation of yeasts, algae and cyanobacteria. Osmotic stress experiments were also carried out by the addition of NaCl. As the most suitable strain for decomposition of the waste fat was selected Sporidiobolus pararoseus, particularly because of its high content of carotenoids, ergosterols and ubiquinone in biomass. Changes in temperature, or increased osmotic pressure had in the most cases a negative effect on biomass production.
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