Original title: Izolace aktivních látek z řas a možnosti jejich aplikace v kosmetických přípravcích
Translated title: Isolation of active compounds from algae and their application in cosmetic products
Authors: Nováková, Laura ; Byrtusová, Dana (referee) ; Skoumalová, Petra (advisor)
Document type: Bachelor's theses
Year: 2019
Language: slo
Publisher: Vysoké učení technické v Brně. Fakulta chemická
Abstract: [slo] [eng]
Hlavným cieľom bakalárskej práce bolo štúdium obsahu aktívnych látok a antioxidačných účinkov vypestovaných mikrorias. Porovnali sme výsledky s komerčnou riasou, ktorá je voľne dostupná na trhu. V teoretickej časti bola zhrnutá problematika využitia metabolitov rias v kozmetike a stručná charakteristika použitých metód pri ich testovaní. V experimentálnej časti boli charakterizované 3 typy extraktov (vodné, alkoholové a olejové) zo 4 druhov rias z hľadiska obsahu celkových polyfenolov, flavonoidov a antioxidačnej aktivity. Najviac aktívnych látok obsahovali práve olejové extrakty. Všetky extrakty boli enkapsulované do lipozómov. Priemerná veľkosť nanočastíc sa pohybovala okolo 200 až 300 nm. Častice boli dostatočné stabilné aj z hľadiska dlhodobej stability. Najväčší pokles bol zaznamenaný pri olejovom extrakte vypestovanej riasy Chlorella vulgaris. Olejové lipozómy boli podrobené MTT testu cytotoxicity na bunkovej kultúre HaCat. Na záver bolo navrhnuté možné použitie pripravených lipozómov v anti-aging kozmetickom priemysle. The bachelor thesis is dedicated to the study of active substances and antioxidant effects of cultivated microalgae. We compared the results with a commercial alga that is freely available. In the theoretical part we focused on description of algal metabolites in cosmetics and a brief description of the methods used in their testing. In the experimental part there were analyzed three types of extracts (aqueous, alcohol and oil) from 4 species of algae. It is mainly the content of polyphenols, flavonoids and antioxidant activity that is determined. According to the results, the highest content of active substances was found in oil extracts. All extracts were encapsulated into liposomes. The average size of the nanoparticles was in range of 200 nm to 300 nm. The particles were stable enough also for long-term stability. The largest decrease was observed in the oil extract of the cultivated algae Chlorella vulgaris. The cytotoxicity of oil extracts was realized by MTT test on human cell cultures HaCaT. Finally, we concluded that the prepared liposomal particles could be used for anti-aging cosmetics.
Keywords: Algae; antioxidant; biomass; cosmetics; cyanobacteria; emulsion; encapsulation; extracts; liposomes

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/173427

Permalink: http://www.nusl.cz/ntk/nusl-401128


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Universities and colleges > Public universities > Brno University of Technology
Academic theses (ETDs) > Bachelor's theses