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Influence of water deficit on physiological characteristics of selected species of green leafy vegetables
Kraus, Kamil ; Hnilička, František (advisor) ; Dana, Dana (referee)
The harmful influences on plants are called stress factors. The stress itself does not slow down only vital functions of plants but influences directly the size and quality of final product as well.
Worldwide, we count the so called abiotic stresses as the most significant crop production stressors. These include in recent years extremely high temperatures and unequal rainfall distribution both areal and during vegetation period.
The water deficit (drought) belongs to the most important stress factors in agriculture. It influences crop production all over the world. Also vegetables are responsive to water deficit, especially the species with huge amount of water in their tissue. Therefore, the goal of this thesis is to assess the impact of water stress on gas exchange and photosynthetic activity of mangold "Beta vulgaris", salad rocket "Eruca sativa" and lettuce "Lactuca sativa", which were placed at water deficit.
The plants - mangold, salad rocket and lettuce - were grown in a partly controlled greenhouse conditions under the Department of Botany and Plant Physiology FAPPZ ČZU in Prague. The plants were grown at a temperature of 25°C in daytime and 18°C at night under natural light conditions. They were grown in containers of size of 11x11 cm in a mixture of garden substrate A and silica sand in the ration 2:1. The experimental scheme included two variants - control and stressed. The control group of plants was watered, and the second half of plants was left under stress for 22 days, when the water deficit was induced by gradual drying for one month, the experiment took place from 1.6 2015 to 22.6 2015. The speed of gas exchange by plants was measured by apparatus LCpro+. From the values of photosynthesis and transpiration water use efficiency (WUE) and chlorophyll fluorescence were calculated.
The gained results proved difference at reaction to water stress between observed kinds of leaf vegetables.
The speed of photosynthesis and transpiration of observed plant species was measured, which decreased due to impact of water deficit. The average speed of photosynthesis of stressed plants was the lowest by lettuce (11,16 "mikro"mol CO2.m-2.s-1), contrarily the highest by mangold (13,2"mikro"mol CO2.m-2.s-1). In case of control plants was the lowest speed of photosynthesis noticed by lettuce (12,03"mikro"mol CO2.m-2.s-1) and the highest by mangold (14,00"mikro"mol CO2.m-2.s-1). In comparison with control group, the speed of photosynthesis of lettuce decreased significantly by impact of water deficit (difference make 7,23 %). On the other hand the lowest decrease of photosynthesis from stressed plants 5,93% was noticed for mangold.
In case of speed of transpiration, it is possible to note, that the lowest transpiration of control group shows mangold with the average speed of transpiration 1,82 mmol H2O.m-2.s-1, by contrast the highest transpiration was measured by lettuce 3,20 mmol H2O. m-2.s-1. In the case of stressed plants moved the average speed of transpiration between 1,54 mmol H2O.m-2.s-1 for lettuce and 1,79 (mmol H2O.m-2.s-1) for mangold. Also, between control and stressed plants of the same species were found differences, and salad rocket reacted to stress of drought more sensitively and mangold less sensitively. For these two plant species was noted decrease of transpiration in the amount of 23,80% and 1,76%.
From the measured value of photosynthesis and transpiration was calculated water use efficiency (WUE). The calculated values show that stressed plants have higher value of WUE in comparison to control plants. The lowest water efficiency from control plants produced salad rocket
(6.51 x 10-3), while the highest mangold (10.31 x 10-3). In the case of stressed plants was the lowest value of WUE noted for salad rocket (8.96 x 10-3), while for mangold was the highest (10.71 x 10-3).
Chlorophyll fluorescence of the experimental plants was also measured. This characteristic was not influenced by plant species, because in case of control plants, there were no differences found between observed plant species. The lowest difference in value of fluorescence had plants of lettuce (0,69) while the highest mangold and salad rocket (0,7). In conclusion it is possible to note, that more sensitive to water deficit are plants of lettuce. As more tolerant to water deficit seems mangold and salad rocket.
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Influence of growth stimulators on physiological characteristics by hops plants (""Humulus lupulus"" L.)
Bazika, Václav ; Hnilička, František (advisor) ; Josef, Josef (referee)
At growing Saaz variety is the target to reach the highest yield of hop´s cones and high content of alpha acids. The average content of this acids in Saaz variety is in the range from 2,5 to 4,5 %. The growers effort is to reach the higher number of this range because the final selling price is given by content of alpha acids. During the active growth of hops, grower can targeted stimulate the hops to positively affect the physiological processes. Then he can reach better yield of valuable alpha acids and that´s why the target of this diploma thesis was to fix the influence of growth stimulators on physiological processes on the yield of hop´s cones and the yield of alpha acids. And based on the results to identify the suitability of using growth stimulators during the intense hops growing. Following scientific hypothesis were designed: determine the plants reaction diversity on growth stimulator. For reaching the targets and confirm or reject the hypothesis was observed in natural conditions nearby the Chrastany region the plants of Saaz (clone 72) in interaction with the usage of growth stimulators. These stimulators were used: Humastar, Fertileader Vital, Lignoaktivátor. There were four variants: controled with no aplication and three other variants with usage of growth stimulators (for each variant were apllied only one stimulator for three times).
The influence of growth stimulators on hops plants (phase BBCH from 37 to 81) was observed on the basis of the rate of gas exchanges, chlorophyl fluorescence and the leaves chlorophyl content, which were measured by infrared gas analyzer LCpro+ (ADC BioScientific Ltd. Hoddesdon, Great Britain), OS 1-FL (OPTI SCIENCES), Chlorofyl CCM 200 ADC BioScientific Ltd. Hoddesdon, Great Britain) After the crop was evaluated the yield of hop´s cones and the content of alpha acids, beta acids and essentials oils.
The results of measurment indicates that the highest average transpiration rate was observed after Humastar application (+18,3 % against control variant). In the control variant was found the lowest average transpiration rate from all variants. The highest photosynthesis rate was found after Fertileader Vital application (+7 % against control variant). Decrease of photosynthesis rate about 17 % against the control variant was observed after Humastar application. The differences was also observed in chlorophyll fluorescence. The highest Fv/Fm value reached the hop´s plants after Fertileader Vital application, concretely 0,792. On the other hand the lowest Fv/Fm value was observed after Lignoaktivátor application (0,780). After the application of all tested growth stimulators were reached increase of a pigment´s content in hop´s leaves against the control variant. The highest content of pigments were observed after Fertileader Vital application (+19,2 % against control variant). Control variant reached the average content of pigments in hop´s leaves 25,5 chlorophyll units. Differences after the growth stimulation was also observed in yield and quality of dry hop´s cones. The highest yield reached the Lignoaktivátor variant (0,73 t/ha). This is an increase of 17,7 % against the control variant (0,62 t/ha). The yield of hop´s cones was decreased of 8,1 % against the control variant after Humastar application. The similiar trends were also observed in hop´s cones quality. The highest content of alpha acids (3,06 %) was reached after Lignoaktivátor application and at the same time Lignoaktivátor variant also achieved the highest content of essentials oils per 100 g of a sample (0,22 ml). Humastar variant had the lowest content of alpha acids (2,18 %) and also the lowest content of essential oils (0,14 ml).
Based on the results it can be stated that the used growth stimulators had the influence on the physiological characteristics of hop´s plants and also affected the different yield and quality of hop´s cones. Proposed hypothesis were confirmed. Based on the one-year results, application of Fertileader Vital or Lignoaktivátor seems to bee more profitable than Humastar application.
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Assessment of influence of milking with milking robot on selected parameters of dairy welfare
FIALA, Otakar
The goal of this graduation thesis was to find out the influence of milking by automatic milking system (AMS) to the physiological needs of dairy cows within 30 minutes after milking. Ethological study covered the needs for feed intake, water intake and need for lying down. In the next part were obtained data on performance of dairy cows and time data of milking (Preparation time for milking, milking time, and time in the milking box). For determination of an impact of various technologies on dairy cows, the results were compared with the data obtained on the farm with conventional milking technology (auto-tandem parlor). The need of feed intake in 30 minutes after milking was found in the barn with AMS at 75.51 % of dairy cows, in the barn containing auto-tandem parlor it was 84.37 % of dairy cows. Highest rate of silage intake after milking had dairy cows milked by conventional way. The need of water intake was found out in the barn with AMS at 41.79 % of dairy cows and in the barn containing auto-tandem parlor it was 33.45 % of dairy cows. Higher rate of water after milking had dairy cows on the farm using the milking robot. In terms of the needs of feed intake and water within 30 minutes after milking were found in both compared technology almost identical values, 28.23 % on the farm with AMS and 27.27 % on the farm with auto-tandem parlor. From this common intake was observed earlier water intake of animals on both farms of more than 80% percent. The percentage of the cows, which needed to lie down after milking, was found in both technologies very low. On the farm with AMS it was 3.7 % and on the farm with auto-tandem parlor 5.8 %. On the farm, where AMS is used, we could evaluate the physiological needs within 30 minutes after milking in the various parts of a day. The need to feed intake was the highest between 6pm and 12 am) and the lowest in the morning. The need of water intake after milking was highest in the morning between 6am and 12 pm and the lowest between midnight 12am and 6 am. The need of lie down after milking was highest between midnight and 6am and the lowest between noon and 6 pm. Milk yield per milking was 12.89 kg on the farm with AMS and 13.35 kg on the farm with auto-tandem parlor. The average daily milk yield was on the farm with AMS 36.47 kg and 26.7 kg on the farm with auto-tandem parlor. Lower milk yield per milking and higher average milk yield per day on the other hand on the farm with AMS is caused by a higher frequency of milking, among other factors. Preparation time for milking was 2:24 min with AMS and 2:02 with conventional type of milking. The time itself was detected in both methods over 5 minutes. With conventional type of milking the times were longer by almost 30 s (5:41min). The time in milking box was 7:07 min in the technology AMS and 8:16 min in the conventional system of milking. Frequency of using of milking robot during the day reached the lowest rate between 4 pm and 6 pm and between 4 am and 8 am. The increase in the number of milking after 6 pm relates with silage intake at this time. During comparison of analyzed technologies was found insignificant difference in the physiological needs within 30 minutes after milking. For verification of these conclusions it is important to increase amount of farms with conventional milking system.
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Physiological Characteristics of Upper Body Speed-Power Skills in Cross Country Skiing
Matuszná, Lenka ; Heller, Jan (advisor) ; Melichna, Jan (referee)
Title of thesis: Physiological Characteristics of Upper Body Speed-Power Skills in Cross Country Skiing. Aim of the project: Aim of my study was to prepare, develop and evaluate obtained information of anaerobic capacities diagnostic in cross country skiing in different drill periods of the year, and to extract conclusions for training practice of elite trainers and of other coaches. Method: Measurements passed at the biomedical laboratory of Faculty of Physical Education and Sport in the spring, where monitored cross-country skiers passed the maximal test on a running treadmill and Wingate test on a specialized upper-body ergometer. Results will be recorded and subsequently evaluated according to defined methodology. Next measurement was realized on roller skis in terrain. There were evaluated double poling ski techniques. These fitness tests occurred in spring and in autumn. Results: The study documented meaning relevance of physiological diagnostic for cross- country skiers at laboratory and terrain. Physiological capacity of double polling in roller skiing and double polling imitation on ergometer, assessed distance and anaerobic capacity overcome, was statistically significant. The higher cross-country skier had effort, the more he achieved to have distance moved. The relation expressed by the...
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In-home visiting service as part of responsibilities of nurse working in general practitioners office
ŠUPLEROVÁ, Michaela
A significant benefit of the nurse?s visits in patient?s home is mainly the regard for patient?s private space. The patients recover better in home conditions than in hospitals or other social facilities. They are provided with individual care according to their specific needs. The economic factor is also important. The bachelor?s thesis focuses on the extent and benefits of nurse?s visits in a patient?s home and on findings expressing how the patients are satisfied with the services. The questions included in the survey are as follows: What is the extent of the service? Does the attendance in patients? homes mean any contribution for nurses working in the GP?s surgery and for the patients using these services? The research conducted for this thesis shows that the nurses employed in the GP?s surgery for adults who regularly visit their patients are familiar with the entire health documentation of the patients and their families, as well as their social conditions. This is why the care is more effective. Another important factor is a faster sequence of nursing and the following medical care. The patients know the nurse well, they trust her and thus they are usually more open towards her. The research also proves that the patients would prefer more visits of the nurses from their GP?s surgery even during the weekend. They find it discouraging that they are attended by the well-known nurse on weekdays, whereas at the weekend the care is provided by unknown nurses from home care agencies. Another important issue encompasses the demographic differences within the district of the GP for adults, the number of registered patients and the number of nurses employed in the GP?s surgery. The nurses are thus much busier and satisfy mainly the physiological needs of the patients whereas the spiritual needs are neglected. The practical output of the thesis is an information leaflet providing the patients with important facts on the visiting nurse service and its availability.
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Mitochondrial production of reactive oxygen species and its role in physiological regulations
Holzerová, Eliška ; Mráček, Tomáš (advisor) ; Kalous, Martin (referee)
The production of mitochondrial reactive oxygen species and the resulting oxidative stress is an important phenomenon driving long-lasting research and intense discussions. Knowledge of exact mechanisms of reactive oxygen species production and pathways leading to their formation could help us to directly affect their production, a task with potential terapeutic implications. The molecular nature of the production of reactive oxygen species by some enzymes has already been well documented, but others still remain controversial and current theories are obviously far from the truth. Much more interesting is the question of physiological importace of this production. The reactive oxygen species were considered harmful factors clearly distorting the integrity of the organism for a long time. However, recent research suggest that their existence can also be beneficial and effective. Evidently they can serve as a signaling molecules in several metabolic and regulatory pathways occurring in the organism. This bachelor thesis offers insight into the current state of knowledge. It focuses on the most detailed description of the reactive oxygen species production by mitochondrial respiratory chain enzymes. Furthermore, it deals with some signaling cascades, where involvement of mitochondrially generated...
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Endogenous opioids and their physiological significance
Jandová, Gabriela ; Novotný, Jiří (advisor) ; Rudajev, Vladimír (referee)
This study examines the influence of endogenous opioid system on physiological processes. Opioid substances and their receptors are located not only in central but also in peripheral nervous systems, as well as in other tissues. Endogenous opioids are produced in the body and influence a wide-range of physiological functions. They are regulators of pain, emotions, breathing, memory, homeostatic functions, etc. Due to their diversity and variety of effects, they may have many possible medical uses, whether in the field of cardiology, anesthesiology or psychiatry. Since the opioid system influences many physiological processes, the understanding of its properties and functioning is prerequisite for deeper study of those processes. Therefore, the study of opioid system and new discoveries in this field receive a great deal of attention nowadays. Key words: Opioid system, endogenous opioids, receptors, nervous system, physiological functions, pain, emotions, breathing, memory, homeostasis
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Regular Physical Activity in Relation to Physiological Parameters and Weight Reduction in Women
Koubová, Hana ; Cinglová, Lenka (advisor) ; Vařeková, Jitka (referee)
1. Abstrakt Název práce: Pravidelná pohybová činnost ve vztahu k fyziologickým ukazatelům a snižování nadváhy žen. Regular physical aktivity relating to physiological indices and reduction of womens overweight. Cíl práce: V této práci chci porovnat fyziologické ukazatele, hmotnost a dopad na psychiku žen, pravidelně cvičících podle zásad sportovní přípra vy, v rozmezí 6-ti měsíců. Metoda: V rozmezí 6-ti měsíců bylo provedeno laboratorní měření fyzio- logických ukazatelů žen. Průběžně byla sledována hmotnost vybraných žen. Strukturovaným rozhovorem byly zjištěny další informace o znalos- tech a podmínkách pro pohybovou činnost u dalších 20 žen. Výsledky: Sledování potvrdilo hypotézy o špatně prováděné pohybové činnosti žen a jejich přístupu k pohybovým aktivitám. Klíčová slova: obezita, nadváha, tepová frekvence, hmotnost - 2-
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Příjem potravy a metabolická aktivita .i.Galumna elimata./i. (C.L. Koch, 1841) (Acari: Oribatida) při různých teplotách
Šustr, Vladimír ; Hubert, J. ; Pekár, S.
The ability of the physiological compensation of respiration rate and feeding activity for temperature was tested in oribatid mite .i.Galumna elimata./i. to evaluate significance of the increase of amylolytic activity in the whole body homogenates of mite exposed to cold acclimation. Mites were reared on green bark algae (.i.Protococcus viridis./i.) at different constant temperatures (5-30°C) for 21 days. The temperature relationships of respiration rate, presence of animals on food and defecation were compared before acclimation (quick response to temperature change) and after acclimation to constant temperature (effects of adaptation). The relationship of oxygen consumption on temperature was similar in control and acclimated mites. Presence of animals on food increased exponentially with temperature before acclimation. It was higher at 15°C after acclimation at constant temperature.
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