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Effect of ubiquitin-proteasome system on spermiogenesis in mammals
Kroumanová, Kristýna ; Petelák, Aleš (advisor) ; Krejčová, Tereza (referee)
Spermiogenesis is a complicated process in which the haploid spermatids differentiate into morphologically mature sperm. Morphological changes include condensation of chromatin and histone-to-protamine replacement, fusion of Golgi-derived vesicles to form acrosomal cap, flagellum formation, reduction of cytoplasmic volume and organelles rearrangement. Ubiquitin-proteasome system plays a key role in these processes. Ubiquitination is a posttranslational modification, leading to the labelling of intracellular proteins targeted for degradation by 26S proteasome. Importance of sperm ubiquitination, is supported by the fact, that deficiency of ubiquitin-proteasome system can lead to infertility at various degrees. During the subsequent sperm maturation in the epididymis extracellular ubiquitination of abnormal sperm and sperm quality control take place. Ubiquitin-proteasome system plays a key role during fertilization, when the sperm 26S proteasome is co-responsible in zona pellucida penetration. The purpose of this assay is to describe the effect of ubiquitin- proteasome system during different stages of spermiogenesis in mammals.
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Sperm morphology and molecular mechanism associated with sperm fertilizing ability in mice.
Šebková, Nataša ; Hortová, Kateřina (advisor) ; Jonáková, Věra (referee) ; Linhart, Otomar (referee)
The process of sperm capacitation and acrosome reaction (AR) are highly dynamic processes essential for the fertilization, including cytoskeleton proteins in the sperm head. The study of the distribution of actin, spectrin and α-tubulin in the head of representative mammalian sperm indicates that before and after the AR there were changes in the distribution of the cytoskeleton structures in the sperm head of all representatives, mainly in the apical part of the acrosome, but also in the equatorial and postacrosome part of the sperm head. The particularly well-studied actin cytoskeleton plays an important role during the capacitation and before and after the AR. Environmental estrogens can interfere with the function of endogenous hormones in very low concentrations. They can interact with specific receptors affecting several signalling pathways leading to sperm capacitation and AR. The effect of 17β-estradiol, estrone, estriol and synthetic 17α-ethynylestradiol was evaluated by the ratio of tyrosine phosphorylation and the state of the acrosome during in vitro capacitation in the sperm head. This study has provided the evidence that estrogens significantly stimulate capacitation progress in a concentration-dependent manner. Estrogens decrease number of sperm after the induced AR too. The raising...
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Maize fertilizing in terms of selected agricultural company
HOVORKA, Jaroslav
My diploma thesis was focused on the corn nutrition in practical conditions of the agricultural company. The evaluation was made with the help of an experiment, which was made at an altitude of 508 metres, the experimental land belongs to the agricultural company Jasanka s.r.o. The experiment is based on the nutrition of corn with different fertilization doses. Various parameters characterizing plant development were observed during the vegetation season. The final results of this experiment showed the overall differences between the fertilized variants. For all the evaluated indicators, a decreasing tendency with a decreasing amount of nitrogen was confirmed. It is clear that all parameters of the best results were obtained for fertilizer variant 1 (58.5 kg N*ha-1). Similarly, for all parameters, the gradual decrease of values decreasing the fertilizer dose is evident.
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Proposal for a revitalization of greenery in the selected area
NEBESOVÁ, Kateřina
In its theoretical part deals with the importance of green areas in towns and settlements, the history of its inception, its different forms and the importance for a healthy way of life of the urban population. The work follows the influence of green areas on a person in terms of aesthetic and hygienic. Discusses the importance and development of the landscape as well and its effect on human activities and return to the influence of the landscape and the nature of the mental and physical health of a person. The practical part of the thesis, the revitalization of the city's historic park addresses located on the square, Husova. The goal of the revitalization of the Park is to improve the functionality of the reference territory from ecological, functional and aesthetic.
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Vliv teploty na udržení schopnosti oplození a líhnivosti při přechovávání neoplozených jiker u keříčkovce červenolemého
BORŮVKA, Vít
When hormonally induced artificial spawning of african catfish (Clarias gariepinus), was several female injected intraperitoneally in one dose preparation Ovopel at doses of 1.5 pellet × kg-1. Females were kept separately in the tanks at a temperature of 21.5 °C. All females were spawned at the same time latency 19.2 hours. Eggs from three spawned females were mixed and divided into 6 doses. Each batch was placed into thermoboxes at temperature 5 °C, 10 °C, 15 °C, 20 °C, 25 °C and 30 °C. These eggs were stored in thermoboxes and after times of storage 0.5 h, 1 h, 1.5 h, 2 h, 3 h, 4 h, 6 h, 8 h, 10h, part of the eggs (approximately 50 to 100 pieces) were taken out from each thermoboxes in three replications and was placed into individuals cups and fertilized by adding 5 drops of sperm and 20 ml of water. In these samples were subsequently observed fertilization, hatching rate and survival rate. When watching fertilization was in individual temperature the highest values and also statistically non-significant difference ( = 0.05) achieved: at 5 °C in times of fertilization 0.5 2 hrs. (61.6 +- 5.81 % - 47.7 +- 1.48 %), at 10 °C in times 0.5 - 1.5 hrs. (70 +- 6.7 % - 62.1 +- 8.9 %), at 15 °C in times 0.5 - 3 hrs. (59.6 +- 9.4 % - 59.6 +- 2.9 %), at 20 °C in times 0.5 - 3 hrs. (61.4 +- 3.6 % - 56.1 +- 2.5 %), at 25 °C in times 0.5 - 4 hrs. (55.5 +- 7.2 % - 49.7 +- 9.3 %) and at 30 °C in times 0,5 - 3 hrs. (61.6 +- 10.3 % - 51.8 +- 17.8 %). When watching hatching rate was in individual temperature the highest values and also statistically non-significant difference ( = 0.05) achieved: at 5 °C in times of fertilization 0.5 - 1 hrs. (28.4 +- 2.9 % - 21.1 +- 9.5 %), at 10 °C in times 0.5 - 1 hrs. (36.6 +- 17.3 % - 22.1 +- 7 %), at 15 °C in times 0.5 - 2 hrs. (34.1 +- 5.5 % - 26.9 +- 5.1 %), at 20 °C in times 0.5 - 2 hrs. (33 +- 8.2 % - 28.8 +- 1.6 %), at 25 °C in times 0.5 - 4 hrs. (31.4 +- 6.2 % - 15.3 +- 13.5 %) and at 30 °C in times 0.5 - 2 hrs. (33.1 +- 9.2 % - 21.2 +- 8 %). When watching survival rate was in individual temperature the highest values and also statistically non-significant difference ( = 0.05) achieved: at 5 °C in times of fertilization 0.5 - 1 hrs. (20.1 +- 6 % - 13 +- 3.3 %), at 10 °C in times 0.5 - 3 hrs. (19.8 +- 15.31 % - 3.1 +- 3 %), at 15 °C in times 0.5 - 6 hrs. (23.3 +- 9 % - 5 +- 2.8 %), at 20 °C in times 0.5 - 2 hrs. (22.4 +- 1.9 % - 15.1 +- 5.2 %), at 25 °C in times 0.5 - 4 hrs. (18.7 +- 4.4 % - 4.1 +- 1.9 %) and at 30 °C in times 0.5 - 1.5 hrs. (26.2 +- 5.5 % - 21.4 +- 6.8 %). Suitable temperatures for the storage of unfertilized eggs after spawning are two hours before fertilization at temperatures from 15 to 30 °C. Other suitable temperatures which are useful for storage are temperatures 15 to 25 °C, for preservation after 3 hrs. and longer after fertilization.
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The winter wheat growing in terms of selected agricultural company
ŽÁČEK, Josef
This bachelor's thesis deals with the comparsion of three variations of growing winter wheat in practical conditions of a chosen agricultural business. A pilot plant experiment was established in 2015 on the property in cadarstral office Dolní Svince 0802/2 of the total area of 12 ha. For the experiment as such the area of 6 ha was used, which was then divided into three parts, each of the area of 2 ha. During the vegetation period, the presence of weeds was monitored and the yield elements of the individual phases of winter wheat evaluated. After the harvest, the real yield of the grain was evaluated and the final economical appraisal was performed. The average yield of the grain of the first variation (treated by herbicides, fertilized by nitrogen) reached the total of 5,54 t.ha-1 which was a 1,56 t.ha-1 more than the second variation (not treated by herbicides, fertilized by nitrogen). The yield of the second variation was 3,98 t.ha-1. The last established was the third variation (not treated by herbicides, not fertilized by nitrogen) and its yield of the harvested grain was around 2,73 t.ha-1.
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Vliv stanoviště a agrotechniky na produkci fytomasy vybraného klonu topolu (Populus nigra L. X Populus maximowiczii Henry) pro energetické účely
NOVÁKOVÁ, Aneta
The thesis deals with theme of fast-growing species and with the impact of agricultural practices (like choice of location, care and possibility of fertilizers usage) which are the most important factors for successful cultivation of energy tree species. The introductory part of the thesis contains an explanation of the term biomass and its possible distribution by types and ways of obtaining the biomass. Following chapters deal with fast-growing tree species. The issues of cultivation are also described here in parts like basic characteristics, history of these energy species, legislative restrictions and procedure during founding of the plantation itself, selection and preparation of locality, the use of agricultural practices (cultivation, fertilization). The practical part deals with it´s own experiment with a clone of poplar J 105 (Populus nigra L. x Populus maximowiczii Henry). This clone of poplar was planted on four experimental plots. The growth depending on the conditions of habitat and selected agritechnical measures was observed in following years. There are descriptions of conditions on all localities, chosen ways of planting and aftercare about tree vegetation. Another experiment which contained adding different types of nitrogenous fertilizers was performed in the last period of observing. Results showed that the greatest impacts on the quality of the growth of fast-growing trees have properly chosen location and aftercare about tree vegetation, at least in the first vegetation period. Addition of fertilizer seemed as important only in case of planting on nutrient very poor sites.
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Aggregation of bull seminal plasma protein
Boháček, Hanuš ; Liberda, Jiří (advisor) ; Hýsková, Veronika (referee)
Mammalian fertilization is a sequence of unique and fascinating events, during which seminal proteins are of crucial role. In case of bull (Bos taurus), proteins of seminal plasma (BSP), especially its major component PDC-109, are known to be in aggregated forms, but little is known about mechanism of forming aggregates and their biological function. In present thesis we discovered some interesting properties of PDC-109 and BSP proteins. We found that concentration of these proteins influences their aggregation state significantly, which can be of great biological importance. Separation of seminal proteins by size exclusion chromatography revealed three main fractions denoted I, II and III, with apparent molecular weights of Mr > 150 000, Mr = 30 000 and Mr = 13 000, respectively. In case of PDC-109, molecular weights of theese fractions were retained even after purification procedure, which implies very stable interactions in forming of aggregates. In addition, there was a difference in distribution of PDC-109 glycoforms among fractions, which can be related to the fact, that theese fractions have different sperm membrane binding patterns as we determined by fluorescence microscopy. However, further experiments are needed for better understanding this issue.
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Effect of management on the diversity and establishment of plants in the Giant Mountains
Trejbalová, Lenka ; Krahulec, František (advisor) ; Březina, Stanislav (referee)
Meadows, as an integral part of the Giant Mountains, depend on regular management, without it they can get degraded. The aim of this thesis was to evaluate the impact of different types of management (mulching, mowing with or without fertilization) on change in coverage, biomass and establishment of plants. The experiment took place in years 1997-2011 on the site called Sněžné Domky in Giant Mountains on 3 sites which differ in humidity and trophy. The effect of management on vegetation change was statistically conclusive. Mulching was preffered the most by Geranium sylvaticum, mulching and fertilization was preffered especially by these species: Holcus mollis, Alopecurus pratensis and Crepis conyzifolia. Mulching and no fertilization: Achillea millefolium. Avenella flexuosa preferred particularly mowing. Mowing and fertilization was preffered by Festuca rubra, Luzula luzuloides, Anthoxanthum odoratum, Agrostis capillaris, Veronica officinalis and Hieracium lachenalii. Mowing without fertilizazion was preffered by Potentilla erecta. The effect of management on the amount of biomass appeared statistically inconclusive (except for amount of old biomass). Biomass is more correlated by the weather and the trophy of the site than the type of management. The effect of management on establishment of plant...
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